{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T23:25:50Z","timestamp":1776381950691,"version":"3.51.2"},"reference-count":21,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T00:00:00Z","timestamp":1526515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Women's and Children's Hospital Foundation Grant","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The aim of this study was to assess the effect of feeding high protein human milk fortifier (HMF) on growth in preterm infants. In this single-centre randomised trial, 60 infants born 28\u201332 weeks\u2019 gestation were randomised to receive a higher protein HMF providing 1.8 g protein (n = 31) or standard HMF providing 1 g protein per 100 mL expressed breast milk (EBM) (n = 29). The primary outcome was rate of weight gain. Baseline characteristics were similar between groups. There was no difference between high and standard HMF groups for weight gain (mean difference (MD) \u221214 g\/week; 95% CI \u221232, 4; p = 0.12), length gain (MD \u22120.01 cm\/week; 95% CI \u22120.06, 0.03; p = 0.45) or head circumference gain (MD 0.007 cm\/week; 95% CI \u22120.05, 0.06; p = 0.79), despite achieving a 0.7 g\/kg\/day increase in protein intake in the high protein group. Infants in the high protein group had a higher proportion of lean body mass at trial entry; however, there was no group by time effect on lean mass gains over the study. Increasing HMF protein content to 1.8 g per 100 mL EBM does not improve growth in preterm infants born 28\u201332 weeks\u2019 gestation.<\/jats:p>","DOI":"10.3390\/nu10050634","type":"journal-article","created":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T11:47:45Z","timestamp":1526557665000},"page":"634","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Effect of Increasing the Protein Content of Human Milk Fortifier to 1.8 g\/100 mL on Growth in Preterm Infants: A Randomised Controlled Trial"],"prefix":"10.3390","volume":"10","author":[{"given":"Jessica","family":"Reid","sequence":"first","affiliation":[{"name":"Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, SA 5006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3832-541X","authenticated-orcid":false,"given":"Maria","family":"Makrides","sequence":"additional","affiliation":[{"name":"Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, SA 5006, Australia"},{"name":"Adelaide Medical School, Discipline of Paediatrics, The University of Adelaide, Adelaide, SA 5006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"McPhee","sequence":"additional","affiliation":[{"name":"Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, SA 5006, Australia"},{"name":"Neonatal Medicine, Women\u2019s and Children\u2019s Hospital, Adelaide, SA 5006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Stark","sequence":"additional","affiliation":[{"name":"Neonatal Medicine, Women\u2019s and Children\u2019s Hospital, Adelaide, SA 5006, Australia"},{"name":"The Robinson Research Institute, The University of Adelaide, Adelaide, SA 5006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6474-0505","authenticated-orcid":false,"given":"Jacqueline","family":"Miller","sequence":"additional","affiliation":[{"name":"Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, SA 5006, Australia"},{"name":"Nutrition and Dietetics, Flinders University, Adelaide, SA 5006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmel T.","family":"Collins","sequence":"additional","affiliation":[{"name":"Healthy Mothers, Babies and Children, South Australian Health and Medical Research Institute, Adelaide, SA 5006, Australia"},{"name":"Adelaide Medical School, Discipline of Paediatrics, The University of Adelaide, Adelaide, SA 5006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.clp.2014.02.010","article-title":"Fortification of human milk in very low birth weight infants (VLBW <1500 g birth weight)","volume":"41","author":"Adamkin","year":"2014","journal-title":"Clin. Perinatol."},{"key":"ref_2","first-page":"S16","article-title":"Human milk in feeding premature infants: Consensus statement","volume":"61","author":"Moro","year":"2015","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Brown, J.V.E., Embleton, N.D., Harding, J.E., and McGuire, W. (2016). Multi-nutrient fortification of human milk for preterm infants. Cochrane Database Syst. Rev.","DOI":"10.1002\/14651858.CD000343.pub3"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1097\/MPG.0b013e3181adaee0","article-title":"Enteral nutrient supply for preterm infants: Commentary from the European Society of Paediatric Gastroenterology, Hepatology and Nutrition committee on nutrition","volume":"50","author":"Agostoni","year":"2010","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Colaizy, T.T., Carlson, S., Saftlas, A.F., and Morriss, F.H. (2012). Growth in vlbw infants fed predominantly fortified maternal and donor human milk diets: A retrospective cohort study. BMC Pediatr., 12.","DOI":"10.1186\/1471-2431-12-124"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1542\/peds.107.2.270","article-title":"Postnatal malnutrition and growth retardation: An inevitable consequence of current recommendations in preterm infants?","volume":"107","author":"Embleton","year":"2001","journal-title":"Pediatrics"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Maas, C., Wiechers, C., Bernhard, W., Poets, C.F., and Franz, A.R. (2013). Early feeding of fortified breast milk and in-hospital-growth in very premature infants: A retrospective cohort analysis. BMC Pediatr., 13.","DOI":"10.1186\/1471-2431-13-178"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/jp.2014.143","article-title":"Association of metabolic acidosis with bovine milk-based human milk fortifiers","volume":"35","author":"Cibulskis","year":"2015","journal-title":"J. Perinatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/sj.jp.7211571","article-title":"Adjustable fortification of human milk fed to preterm infants: Does it make a difference?","volume":"26","author":"Arslanoglu","year":"2006","journal-title":"J. Perinatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.earlhumdev.2013.08.015","article-title":"An intention to achieve better postnatal in-hospital-growth for preterm infants: Adjustable protein fortification of human milk","volume":"89","author":"Alan","year":"2013","journal-title":"Early Hum. Dev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"72","DOI":"10.3109\/14767058.2012.715032","article-title":"Growth and neurological outcome in ELBW preterms fed with human milk and extra-protein supplementation as routine practice: Do we need further evidence?","volume":"25","author":"Biasini","year":"2012","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.jpeds.2013.04.052","article-title":"Target fortification of breast milk with fat, protein, and carbohydrates for preterm infants","volume":"163","author":"Rochow","year":"2013","journal-title":"J. Pediatr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1017\/S0007114515004614","article-title":"Comparing different methods of human breast milk fortification using measured v. Assumed macronutrient composition to target reference growth: A randomised controlled trial","volume":"115","author":"McLeod","year":"2016","journal-title":"Br. J. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"648","DOI":"10.3945\/ajcn.111.026351","article-title":"Effect of increasing protein content of human milk fortifier on growth in preterm infants born at <31 wk gestation: A randomized controlled trial","volume":"95","author":"Miller","year":"2012","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1111\/j.1440-1754.1996.tb00965.x","article-title":"New South Wales population-based birthweight percentile charts","volume":"32","author":"Beeby","year":"1996","journal-title":"J. Paediatr. Child Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1001\/jamapediatrics.2016.2681","article-title":"Effect of increased enteral protein intake on growth in human milk\u2013fed preterm infants: A randomized clinical trial","volume":"171","author":"Maas","year":"2017","journal-title":"JAMA Pediatr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e928","DOI":"10.1542\/peds.2011-3120","article-title":"A new liquid human milk fortifier and linear growth in preterm infants","volume":"130","author":"Moya","year":"2012","journal-title":"Pediatrics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1097\/MPG.0000000000001010","article-title":"Growth and tolerance of preterm infants fed a new extensively hydrolyzed liquid human milk fortifier","volume":"61","author":"Kim","year":"2015","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e83","DOI":"10.1097\/MPG.0000000000001686","article-title":"Growth and nutritional biomarkers of preterm infants fed a new powdered human milk fortifier: A randomized trial","volume":"65","author":"Rigo","year":"2017","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1111\/j.1651-2227.1990.tb11548.x","article-title":"Urinary and serum urea as indicators of protein metabolism in very low birthweight infants fed varying human milk protein intakes","volume":"79","author":"Polberger","year":"1990","journal-title":"Acta Paediatr. Scand."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"S47","DOI":"10.1038\/ejcn.2012.164","article-title":"Prediction of body water compartments in preterm infants by bioelectrical impedance spectroscopy","volume":"67","author":"Collins","year":"2013","journal-title":"Eur. J. Clin. Nutr."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/10\/5\/634\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:04:46Z","timestamp":1760195086000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/10\/5\/634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,17]]},"references-count":21,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2018,5]]}},"alternative-id":["nu10050634"],"URL":"https:\/\/doi.org\/10.3390\/nu10050634","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,17]]}}}