{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:10:19Z","timestamp":1791360619023,"version":"4.3.1"},"reference-count":51,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T00:00:00Z","timestamp":1537228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HE-7032\/1-1"],"award-info":[{"award-number":["HE-7032\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>While the impact of dietary cholesterol on the progression of atherosclerosis has probably been overestimated, increasing evidence suggests that dietary cholesterol might favor the transition from blunt steatosis to non-alcoholic steatohepatitis (NASH), especially in combination with high fat diets. It is poorly understood how cholesterol alone or in combination with other dietary lipid components contributes to the development of lipotoxicity. The current study demonstrated that liver damage caused by dietary cholesterol in mice was strongly enhanced by a high fat diet containing soybean oil-derived \u03c96-poly-unsaturated fatty acids (\u03c96-PUFA), but not by a lard-based high fat diet containing mainly saturated fatty acids. In contrast to the lard-based diet the soybean oil-based diet augmented cholesterol accumulation in hepatocytes, presumably by impairing cholesterol-eliminating pathways. The soybean oil-based diet enhanced cholesterol-induced mitochondrial damage and amplified the ensuing oxidative stress, probably by peroxidation of poly-unsaturated fatty acids. This resulted in hepatocyte death, recruitment of inflammatory cells, and fibrosis, and caused a transition from steatosis to NASH, doubling the NASH activity score. Thus, the recommendation to reduce cholesterol intake, in particular in diets rich in \u03c96-PUFA, although not necessary to reduce the risk of atherosclerosis, might be sensible for patients suffering from non-alcoholic fatty liver disease.<\/jats:p>","DOI":"10.3390\/nu10091326","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T10:50:31Z","timestamp":1537354231000},"page":"1326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Soybean Oil-Derived Poly-Unsaturated Fatty Acids Enhance Liver Damage in NAFLD Induced by Dietary Cholesterol"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1450-5838","authenticated-orcid":false,"given":"Janin","family":"Henkel","sequence":"first","affiliation":[{"name":"Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Alfine","sequence":"additional","affiliation":[{"name":"German Institute of Human Nutrition, Junior Research Group Central Regulation of Metabolism; D-14558 Nuthetal, Germany"},{"name":"German Center for Diabetes Research (DZD), D-85764 M\u00fcnchen-Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4026-5367","authenticated-orcid":false,"given":"Juliana","family":"Sa\u00edn","sequence":"additional","affiliation":[{"name":"Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, D-14558 Nuthetal, Germany"},{"name":"Department of Biological Sciences, Food Science and Nutrition, Faculty of Biochemistry and Biological Sciences, National University of the Litoral (UNL), Santa Fe S3000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korinna","family":"J\u00f6hrens","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Carl Gustav Carus University Hospital Dresden; D-01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, German Institute of Human Nutrition; D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, German Institute of Human Nutrition; D-14558 Nuthetal, Germany"},{"name":"Department of Medicine, Division of Genetics, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3217-573X","authenticated-orcid":false,"given":"Jeannette","family":"K\u00f6nig","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, German Institute of Human Nutrition; D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin","family":"Stuhlmann","sequence":"additional","affiliation":[{"name":"Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madita","family":"Vahrenbrink","sequence":"additional","affiliation":[{"name":"Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenke","family":"Jonas","sequence":"additional","affiliation":[{"name":"German Center for Diabetes Research (DZD), D-85764 M\u00fcnchen-Neuherberg, Germany"},{"name":"Department of Experimental Diabetology, German Institute of Human Nutrition; D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Kleinridders","sequence":"additional","affiliation":[{"name":"German Institute of Human Nutrition, Junior Research Group Central Regulation of Metabolism; D-14558 Nuthetal, Germany"},{"name":"German Center for Diabetes Research (DZD), D-85764 M\u00fcnchen-Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"P\u00fcschel","sequence":"additional","affiliation":[{"name":"Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, D-14558 Nuthetal, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.3892\/br.2017.843","article-title":"Animal models of atherosclerosis","volume":"6","author":"Lee","year":"2017","journal-title":"Biomed. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1017\/S0029665113003844","article-title":"Dietary cholesterol, heart disease risk and cognitive dissonance","volume":"73","author":"McNamara","year":"2014","journal-title":"Pro. Nut. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"276","DOI":"10.3945\/ajcn.114.100305","article-title":"Dietary cholesterol and cardiovascular disease: A systematic review and meta-analysis","volume":"102","author":"Berger","year":"2015","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1753-4887.2010.00294.x","article-title":"Cholesterol: Where science and public health policy intersect","volume":"68","author":"Brownawell","year":"2010","journal-title":"Nutr. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Vinu\u00e9, \u00c1., Herrero-Cervera, A., and Gonz\u00e1lez-Navarro, H. (2018). Understanding the impact of dietary cholesterol on chronic metabolic diseases through studies in rodent models. Nutrients, 10.","DOI":"10.3390\/nu10070939"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Jahn, D., Kircher, S., Hermanns, H.M., and Geier, A. (2018). Animal models of NAFLD from a hepatologist\u2019s point of view. BBA-Mol. Basis. Dis.","DOI":"10.1016\/j.bbadis.2018.06.023"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/hep.29466","article-title":"Modeling the epidemic of nonalcoholic fatty liver disease demonstrates an exponential increase in burden of disease","volume":"67","author":"Estes","year":"2018","journal-title":"Hepatology"},{"key":"ref_8","first-page":"20","article-title":"Nonalcoholic fatty liver disease: Epidemiology, pathogenesis and therapeutic implications","volume":"11","author":"Andronescu","year":"2018","journal-title":"J. Med. Life"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15080","DOI":"10.1038\/nrdp.2015.80","article-title":"Nonalcoholic fatty liver disease","volume":"1","author":"Brunt","year":"2015","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1194\/jlr.M016246","article-title":"Dietary cholesterol exacerbates hepatic steatosis and inflammation in obese LDL receptor-deficient mice","volume":"52","author":"Subramanian","year":"2011","journal-title":"J. Lipid Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"70","DOI":"10.2119\/molmed.2016.00203","article-title":"Induction of steatohepatitis (NASH) with insulin resistance in wildtype B6 mice by a western-type diet containing soybean oil and cholesterol","volume":"23","author":"Henkel","year":"2017","journal-title":"Mol. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1194\/jlr.M072454","article-title":"Cholesterol crystallization within hepatocyte lipid droplets and its role in murine NASH","volume":"58","author":"Ioannou","year":"2017","journal-title":"J. Lipid Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1055\/s-0033-1353209","article-title":"Olive oil attenuates the cholesterol-induced development of nonalcoholic steatohepatitis despite increased insulin resistance in a rodent model","volume":"45","author":"Buettner","year":"2013","journal-title":"Horm. Metab. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1016\/j.drudis.2017.06.007","article-title":"Mouse models of nonalcoholic steatohepatitis in preclinical drug development","volume":"22","author":"Hansen","year":"2017","journal-title":"Drug Discov. Today"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.3892\/mmr.2014.2623","article-title":"Ezetimibe prevents the development of non-alcoholic fatty liver disease induced by high-fat diet in C57BL\/6J mice","volume":"10","author":"Wang","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1111\/hepr.12887","article-title":"Ezetimibe for the treatment of non-alcoholic fatty liver disease: A meta-analysis","volume":"47","author":"Nakade","year":"2017","journal-title":"Hepatol. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2018\/2548154","article-title":"Hypoxic signaling and cholesterol lipotoxicity in fatty liver disease progression","volume":"7","author":"Tirosh","year":"2018","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.tem.2015.11.008","article-title":"The Role of Cholesterol in the Pathogenesis of NASH","volume":"27","author":"Ioannou","year":"2016","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_19","unstructured":"Siedel, J., Rollinger, W., R\u00f6schlau, P., Ziegenhorn, J., and Bergmeyer, H.U. (1984). Total cholesterol, end-point and kinetic method. Methods of Enzymatic Analysis, Verlag Chemie GmbH."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1002\/hep.20701","article-title":"Design and validation of a histological scoring system for nonalcoholic fatty liver disease","volume":"41","author":"Kleiner","year":"2005","journal-title":"Hepatology"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liang, W., Menke, A.L., Driessen, A., Koek, G.H., Lindeman, J.H., Stoop, R., Havekes, L.M., Kleemann, R., and van den Hoek, A.M. (2014). Establishment of a general NAFLD scoring system for rodent models and comparison to human liver pathology. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115922"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"E938","DOI":"10.1152\/ajpendo.00427.2015","article-title":"Insulin-induced cytokine production in macrophages causes insulin resistance in hepatocytes","volume":"310","author":"Manowsky","year":"2016","journal-title":"Am. J. Physiol. Endocrin. Met."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/hep.23064","article-title":"Aggravation by prostaglandin E2 of interleukin-6-dependent insulin resistance in hepatocytes","volume":"50","author":"Henkel","year":"2009","journal-title":"Hepatology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1007\/s10620-009-0815-3","article-title":"Characterization of high-fat, diet-induced, non-alcoholic steatohepatitis with fibrosis in rats","volume":"55","author":"Xu","year":"2010","journal-title":"Digest. Dis. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1002\/hep.21874","article-title":"Lipid-induced oxidative stress causes steatohepatitis in mice fed an atherogenic diet","volume":"46","author":"Matsuzawa","year":"2007","journal-title":"Hepatology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0021-9150(77)90020-X","article-title":"Type and amount of dietary fat affect relative concentration of cholesterol in blood and other tissues of rats","volume":"27","author":"Wiggers","year":"1977","journal-title":"Atherosclerosis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.atherosclerosis.2008.08.021","article-title":"Impact of feeding polyunsaturated fatty acids on cholesterol metabolism of dyslipidemic obese rats of WNIN\/GR-Ob strain","volume":"204","author":"Sheril","year":"2009","journal-title":"Atherosclerosis"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Schumacher, T., and Benndorf, R.A. (2017). ABC transport proteins in cardiovascular disease\u2014A brief summary. Molecules, 22.","DOI":"10.3390\/molecules22040589"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Pizzini, A., Lunger, L., Demetz, E., Hilbe, R., Weiss, G., Ebenbichler, C., and Tancevski, I. (2017). The role of omega-3 fatty acids in reverse cholesterol transport: A review. Nutrients, 9.","DOI":"10.3390\/nu9101099"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1093\/jn\/126.7.1759","article-title":"A high cholesterol, (n-3) polyunsaturated fatty acid diet induces hypercholesterolemia more than a high cholesterol (n-6) polyunsaturated fatty acid diet in hamsters","volume":"126","author":"Lu","year":"1996","journal-title":"J. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.clnu.2013.04.009","article-title":"Lipid content in hepatic and gonadal adipose tissue parallel aortic cholesterol accumulation in mice fed diets with different omega-6 PUFA to EPA plus DHA ratios","volume":"33","author":"Wang","year":"2014","journal-title":"Clin. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S002966511500244X","article-title":"Impact of dietary fat on the development of non-alcoholic fatty liver disease in Ldlr\u2212\/\u2212 mice","volume":"75","author":"Jump","year":"2016","journal-title":"Proc. Nutr. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/s12944-017-0461-2","article-title":"Protective effects of various ratios of DHA\/EPA supplementation on high-fat diet-induced liver damage in mice","volume":"16","author":"Shang","year":"2017","journal-title":"Lipids Health Dis."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pachikian, B.D., Essaghir, A., Demoulin, J.B., Neyrinck, A.M., Catry, E., de Backer, F.C., Dejeans, N., Dewulf, E.M., Sohet, F.M., and Portois, L. (2011). Hepatic n-3 polyunsaturated fatty acid depletion promotes steatosis and insulin resistance in mice: Genomic analysis of cellular targets. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0023365"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Schwarz, J., Tom\u00e9, D., Baars, A., Hooiveld, G.J., and M\u00fcller, M. (2012). Dietary protein affects gene expression and prevents lipid accumulation in the liver in mice. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047303"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1042\/CS20140026","article-title":"Endoplasmic reticulum stress does not contribute to steatohepatitis in obese and insulin-resistant high-fat-diet-fed foz\/foz mice","volume":"127","author":"Legry","year":"2014","journal-title":"Clin. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3389\/fphar.2014.00151","article-title":"Glutathione and mitochondria","volume":"5","author":"Ribas","year":"2014","journal-title":"Front. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.cmet.2006.07.006","article-title":"Mitochondrial free cholesterol loading sensitizes to TNF- and Fas-mediated steatohepatitis","volume":"4","author":"Caballero","year":"2006","journal-title":"Cell Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.bbalip.2009.09.011","article-title":"Mitochondrial dysfunction and lipotoxicity","volume":"1801","author":"Schrauwen","year":"2010","journal-title":"BBA-Mol. Cell Biol. Lipids"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.1016\/j.freeradbiomed.2014.10.594","article-title":"Oxysterols induce mitochondrial impairment and hepatocellular toxicity in non-alcoholic fatty liver disease","volume":"75","author":"Bellanti","year":"2014","journal-title":"Free Radic. Biol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jnutbio.2007.12.001","article-title":"Phospholipidosis and down-regulation of the PI3-K\/PDK-1\/Akt signalling pathway are vitamin E inhibitable events associated with 7-ketocholesterol-induced apoptosis","volume":"20","author":"Vejux","year":"2009","journal-title":"J. Nutr. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1007\/s00535-012-0585-0","article-title":"Increased serum liver X receptor ligand oxysterols in patients with non-alcoholic fatty liver disease","volume":"47","author":"Ikegami","year":"2012","journal-title":"J. Gastroenterol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3109\/10715762.2013.835048","article-title":"Oxysterols and redox signaling in the pathogenesis of non-alcoholic fatty liver disease","volume":"47","author":"Serviddio","year":"2013","journal-title":"Free Radic. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1194\/jlr.M034876","article-title":"Hepatic cholesterol crystals and crown-like structures distinguish NASH from simple steatosis","volume":"54","author":"Ioannou","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nrgastro.2017.38","article-title":"Mechanisms of hepatic stellate cell activation","volume":"14","author":"Tsuchida","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepat."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"G553","DOI":"10.1152\/ajpgi.00547.2007","article-title":"Increased susceptibility to exacerbated liver injury in hypercholesterolemic ApoE-deficient mice: Potential involvement of oxysterols","volume":"296","author":"Horrillo","year":"2009","journal-title":"Am. J. Physiol. Gastr. Liver Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/hep.22941","article-title":"Association between dietary nutrient composition and the incidence of cirrhosis or liver cancer in the United States population","volume":"50","author":"Ioannou","year":"2009","journal-title":"Hepatology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1053\/jhep.2003.50132","article-title":"Dietary habits and their relations to insulin resistance and postprandial lipemia in nonalcoholic steatohepatitis","volume":"37","author":"Musso","year":"2003","journal-title":"Hepatology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1080\/00365520802588133","article-title":"Nutritional investigation of non-obese patients with non-alcoholic fatty liver disease: The significance of dietary cholesterol","volume":"44","author":"Yasutake","year":"2009","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lee, H.Y., Jun, D.W., Kim, H.J., Oh, H., Saeed, W.K., Ahn, H., Cheung, R.C., and Nguyen, M.H. (2018). Ezetimibe decreased nonalcoholic fatty liver disease activity score but not hepatic steatosis. Korean J. Int. Med.","DOI":"10.3904\/kjim.2017.194"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"He, X.X., Wu, X.L., Chen, R.P., Chen, C., Liu, X.G., Wu, B.J., and Huang, Z.M. (2016). Effectiveness of omega-3 polyunsaturated fatty acids in non-alcoholic fatty liver disease: A meta-analysis of randomized controlled trials. 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