{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:28:47Z","timestamp":1761395327524,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,9,20]],"date-time":"2018-09-20T00:00:00Z","timestamp":1537401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit-2 237 leucine\/methionine (ND2-237 Leu\/Met) polymorphism has been shown to modify the association of coffee consumption with the risk of hypertension, dyslipidemia, and abnormal glucose tolerance, and low serum chloride levels have been shown to be associated with all-cause and cardiovascular disease mortality. Therefore, the purpose of the present study was to investigate whether ND2-237 Leu\/Met polymorphism influences the association of coffee consumption with serum chloride levels in male Japanese health checkup examinees. Methods: From among individuals visiting the hospital for a regular medical checkup, 402 men (mean age \u00b1 standard deviation, 53.9 \u00b1 7.8 years) were selected for inclusion in the study. After ND2-237 Leu\/Met genotyping, we conducted an exploratory cross-sectional study to examine the combined association of ND2-237 Leu\/Met polymorphism and coffee consumption with serum electrolyte levels. Results: After adjusting for age, body mass index, habitual smoking, alcohol consumption, green tea consumption, and antihypertensive medication, coffee consumption significantly increased serum chloride levels (p for trend = 0.001) in men with the ND2-237Leu genotype. After these adjustments, the odds ratios (ORs) for low levels of serum chloride, defined as &lt;100 mEq\/L, were found to be dependent on coffee consumption (p for trend = 0.001). In addition, the OR for low levels of serum chloride was significantly lower in men with the ND2-237Leu genotype who consumed \u22654 compared with &lt;1 cup of coffee per day (OR = 0.096, 95% confidence interval = 0.010\u20130.934; p = 0.044). However, neither serum chloride levels nor risk of low levels of serum chloride appeared to be dependent on coffee consumption. Conclusions: The results suggest that ND2-237 Leu\/Met polymorphism modifies the association of coffee consumption with serum chloride levels in middle-aged Japanese men.<\/jats:p>","DOI":"10.3390\/nu10101344","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T03:46:35Z","timestamp":1537501595000},"page":"1344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["NADH Dehydrogenase Subunit-2 237 Leu\/Met Polymorphism Influences the Association of Coffee Consumption with Serum Chloride Levels in Male Japanese Health Checkup Examinees: An Exploratory Cross-Sectional Analysis"],"prefix":"10.3390","volume":"10","author":[{"given":"Akatsuki","family":"Kokaze","sequence":"first","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamoru","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan"},{"name":"Mito Red Cross Hospital, 3-12-48 Sannomaru, Mito-shi, Ibaraki 310-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanae","family":"Karita","sequence":"additional","affiliation":[{"name":"Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotaka","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takako","family":"Shirasawa","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-6179","authenticated-orcid":false,"given":"Takahiko","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9805-508X","authenticated-orcid":false,"given":"Akira","family":"Minoura","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Oikawa","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Satoh","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Health, Faculty of Health and Medical Care, Saitama Medical University, 1397-1 Yamane, Hidaka-shi, Saitama 350-1241, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Hoshino","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Takashima","sequence":"additional","affiliation":[{"name":"Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.maturitas.2013.02.002","article-title":"The impact of coffee on health","volume":"75","author":"Tarin","year":"2013","journal-title":"Maturitas"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/B978-0-12-398397-8.00012-5","article-title":"Coffee intake","volume":"108","author":"Cornelis","year":"2012","journal-title":"Prog. 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