{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T10:40:22Z","timestamp":1787308822717,"version":"build-2736575974"},"reference-count":56,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC98-2321-B-019-002"],"award-info":[{"award-number":["NSC98-2321-B-019-002"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Hot water was used to obtain Chlorella sorokiniana hot water extract (HWE). Subsequently, this byproduct was freeze-dried, hydrolysed at 50 \u00b0C using Protease N to obtain C. sorokiniana protein hydrolysates (PN-1), and then digested with a gastrointestinal enzyme (PN-1G). The inhibitory effects of the HWE and hydrolysates against angiotensin I-converting enzyme (ACE) were investigated. The soluble protein and peptide contents were 379.9 and 179.7 mg\/g, respectively, for HWE and 574.8 and 332.8 mg\/g, respectively, for PN-1. The IC50 values of the HWE, PN-1, and PN-1G on ACE were 1.070, 0.035, and 0.044 mg\/mL, respectively. PN-1G was separated into seven fractions through size exclusion chromatography. The sixth fraction of the hydrolysate had a molecular weight between 270 and 340 Da, and the lowest IC50 value on ACE was 0.015 mg\/mL. The amino acid sequences of the ACE-inhibitory peptides were Trp-Val, Val-Trp, Ile-Trp, and Leu-Trp, of which the IC50 values were 307.61, 0.58, 0.50, and 1.11 \u00b5\u039c, respectively. Systolic blood pressure and diastolic blood pressure were reduced 20 and 21 mm Hg, respectively, in spontaneously hypertensive rats after 6 h of oral administration with a dose of 171.4 mg PN-1 powder\/kg body weight.<\/jats:p>","DOI":"10.3390\/nu10101397","type":"journal-article","created":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T08:23:50Z","timestamp":1538468630000},"page":"1397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides and the Antihypertensive Effect of Chlorella sorokiniana Protein Hydrolysates"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9908-6304","authenticated-orcid":false,"given":"Yu-Hsin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Food Technology and Marketing, Taipei University of Marine Technology, No. 212, Section 9, Yan Ping North Road, Taipei 111, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan-Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Food Science, National Taiwan Ocean University, No. 2 Pei-Ning Road, Keelung 202, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin","family":"Yeh","sequence":"additional","affiliation":[{"name":"Taiwan Chlorella Manufacturing Co., Ltd., 5 F, No. 71, Section 2, Nan-King East Road, Taipei 104, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Song","sequence":"additional","affiliation":[{"name":"Taiwan Chlorella Manufacturing Co., Ltd., 5 F, No. 71, Section 2, Nan-King East Road, Taipei 104, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenn-Shou","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Food Science, National Taiwan Ocean University, No. 2 Pei-Ning Road, Keelung 202, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.foodchem.2008.12.019","article-title":"Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste","volume":"115","author":"Sheih","year":"2009","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.foodchem.2008.03.059","article-title":"Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the antihypertensive effect of hydrolysate in spontaneously hypertensive rats","volume":"111","author":"Wang","year":"2008","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1126\/science.191908","article-title":"Design of specific inhibitors of angiotensin-converting enzyme: New class of orally active antihypertensive agents","volume":"196","author":"Ondetti","year":"1977","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1248\/cpb.38.529","article-title":"Synthesis of 1-[(S)-3-acetylthio-2-methylpropanoyl]-l-phenylalanine (Alacepril) and one of its active metabolites, the desacetyl derivative (DU-1227)","volume":"38","author":"Sawayama","year":"1990","journal-title":"Chem. 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