{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:49:13Z","timestamp":1764784153432,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL 2011-29644-C02-02 and AGL 2014-53207-C2-2-R"],"award-info":[{"award-number":["AGL 2011-29644-C02-02 and AGL 2014-53207-C2-2-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Restructuring pork (RP) by adding new functional ingredients, like Chia oil (one of the richest natural source of \u03b1-linolenic acid) or hydroxytyrosol (HxT) (potent antioxidant), both with hypolipidemic activities, is one of the strategies that may help to reduce the potential negative effects of high meat products consumption. The aim of this study was to evaluate the Chia oil- or HxT-enriched-RP effect on the lipoprotein profile of aged rats fed high-fat, high-energy, and cholesterol-enriched diets. RP samples were prepared by mixing lean pork and lard with or without Chia oil (152.2 g\/kg fresh matter) or HxT (3.6 g\/kg fresh matter). Diets were prepared by mixing a semisynthetic diet with freeze-dried RP. Groups of 1-year male Wistar rats were fed the following experimental diets for 8 weeks: C, control-RP diet; HC, cholesterol-enriched-RP diet; and Chia oil-RP (CHIA) and HxT, Chia oil- or hydroxytyrosol-RP, cholesterol-enriched diet. Plasma lipid, lipoprotein profile, SREBP-1c protein, and low-density lipoproteins (LDL) receptor gene (Ldlr) expressions were evaluated. Compared to C diet, the HC diet increased plasma cholesterol, triglycerides, free fatty acids, total lipids, and SREBP-1c expression, but reduced Ldlr expression and significantly modified the lipoprotein profile, giving rise to the presence of high levels of atherogenic cholesterol-enriched very low-density lipoproteins (VLDL) particles. Compared to the HC diet, the HxT diet did not produce significant changes in feed intake but it reduced the body weight. Chia oil and HxT partially arrested the negative effects of the high-fat, high-energy, and cholesterol-enriched meat-based diets on lipemia and lipoproteinemia, mostly by reducing the amount of cholesterol content in VLDL (60% and 74% less in CHIA and HxT vs. HC, respectively) and the VLDL total mass (59% and 63% less in CHIA and HxT vs. HC, respectively). Free fatty acids (FFA) significantly correlated with adipose tissue weight and VLDL total mass (both p &lt; 0.05), and plasma triglycerides, phospholipids, total lipids, and SREBP-1c (all p &lt; 0.001), suggesting the important role of FFA in lipoprotein metabolism. Results support the recommendation to include these ingredients in pork products addressed to reduce the presence of increased atherogenic particles in aged people at CVD risk consuming large amounts of pork.<\/jats:p>","DOI":"10.3390\/nu10121830","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T03:31:33Z","timestamp":1543289493000},"page":"1830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Lipoprotein Profile in Aged Rats Fed Chia Oil- or Hydroxytyrosol-Enriched Pork in High Cholesterol\/High Saturated Fat Diets"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4924-0809","authenticated-orcid":false,"given":"Jorge Arturo","family":"Santos-L\u00f3pez","sequence":"first","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Farmacognosia y Bot\u00e1nica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"Garcimart\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Farmacognosia y Bot\u00e1nica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Bastida","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Ciencia de los Alimentos, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6725-270X","authenticated-orcid":false,"given":"Mirandeli","family":"Bautista-\u00c1vila","sequence":"additional","affiliation":[{"name":"\u00c1rea Acad\u00e9mica de Farmacia, Instituto de Ciencias de la Salud, Universidad Aut\u00f3noma del Estado de Hidalgo, Ex Hacienda la Concepci\u00f3n s\/n, Ctra. Pachuca-Tilcuautla, Hidalgo 42060, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2383-3788","authenticated-orcid":false,"given":"Mar\u00eda Jos\u00e9","family":"Gonz\u00e1lez-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Biom\u00e9dicas, Unidad Docente de Toxicolog\u00eda, Facultad de Farmacia, Universidad de Alcal\u00e1, Ctra. Madrid-Barcelona km, 33,600, 28805 Alcal\u00e1 de Henares, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juana","family":"Bened\u00ed","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Farmacognosia y Bot\u00e1nica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2660-5126","authenticated-orcid":false,"given":"Francisco Jos\u00e9","family":"S\u00e1nchez-Muniz","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Ciencia de los Alimentos, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.20960\/nh.29","article-title":"To eat or not to eat meat. That is the question","volume":"33","author":"Celada","year":"2016","journal-title":"Nutr. Hosp."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.foodchem.2010.05.104","article-title":"Design and development of meat-based functional foods with walnut: Technological, nutritional and health impact","volume":"123","year":"2010","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.meatsci.2013.03.030","article-title":"Development and assessment of healthy properties of meat and meat products designed as functional foods","volume":"95","year":"2013","journal-title":"Meat Sci."},{"key":"ref_4","first-page":"919","article-title":"Coagulation, thrombogenesis, and insulin resistance markers in increased-cardiovascular-risk subjects consuming improved-fat meat products","volume":"95","author":"Celada","year":"2018","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1089\/jmf.2013.0100","article-title":"Protective effects of sea spaghetti-enriched restructured pork against dietary cholesterol: Effects on arylesterase and lipoprotein profile and composition of growing rats","volume":"17","author":"Schultz","year":"2014","journal-title":"J. Med. Food"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00394-016-1299-5","article-title":"Dietary salba (Salvia hispanica L.) ameliorates the adipose tissue dysfunction of dyslipemic insulin-resistant rats through mechanisms involving oxidative stress, inflammatory cytokines and peroxisome proliferator-activated receptor \u03b3","volume":"57","author":"Ferreira","year":"2018","journal-title":"Eur. J. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Creus, A., Ferreira, M.R., Oliva, M.E., and Lombardo, Y.B. (2016). Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary alpha-linolenic acid rich Salvia hispanica L. (salba) seed in the heart of dyslipemic insulin-resistant rats. J. Clin. Med., 5.","DOI":"10.3390\/jcm5020018"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1039\/C3FO60688K","article-title":"Alpha linolenic acid (ALA) from rosa canina, sacha inchi and chia oils may increase ALA accretion and its conversion into n-3 LCPUFA in diverse tissues of the rat","volume":"5","author":"Valenzuela","year":"2014","journal-title":"Food Funct."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1159\/000100818","article-title":"Effect of dietary \u03b1-linolenic fatty acid derived from chia when fed as ground seed, whole seed and oil on lipid content and fatty acid composition of rat plasma","volume":"51","author":"Ayerza","year":"2007","journal-title":"Ann. Nutr. Metab."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1017\/S000711450899053X","article-title":"Dietary chia seed (Salvia hispanica L.) rich in \u03b1-linolenic acid improves adiposity and normalises hypertriacylglycerolaemia and insulin resistance in dyslipaemic rats","volume":"101","author":"Chicco","year":"2009","journal-title":"Br. J. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1017\/S0007114512003558","article-title":"Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats","volume":"109","author":"Rossi","year":"2013","journal-title":"Br. J. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3334","DOI":"10.1021\/jf073403s","article-title":"Hydroxytyrosol prevents oxidative deterioration in foodstuffs rich in fish lipids","volume":"56","author":"Pazos","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/j.lwt.2014.06.051","article-title":"Oxidative stability of meat systems made with W1\/O\/W2 emulsions prepared with hydroxytyrosol and chia oil as lipid phase","volume":"59","author":"Cofrades","year":"2014","journal-title":"LWT Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Santos-L\u00f3pez, J.A., Garcimart\u00edn, A., Merino, P., L\u00f3pez-Oliva, M.E., Bastida, S., Bened\u00ed, J., and S\u00e1nchez-Muniz, F.J. (2016). Effects of silicon vs. hydroxytyrosol-enriched restructured pork on liver oxidation status of aged rats fed high-saturated\/high-cholesterol diets. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0147469"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1021\/jf405820v","article-title":"Hydroxytyrosol and its potential therapeutic effects","volume":"62","author":"Hu","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.freeradbiomed.2013.11.029","article-title":"Hydroxytyrosol prevents diet-induced metabolic syndrome and attenuates mitochondrial abnormalities in obese mice","volume":"67","author":"Cao","year":"2014","journal-title":"Free Rad. Biol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s003940050042","article-title":"Small supplements of n-3 fatty acids change serum low density lipoprotein composition by decreasing phospholipid and apolipoprotein B concentrations in young adult women","volume":"38","author":"Bastida","year":"1999","journal-title":"Eur. J. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.3945\/jn.116.243204","article-title":"Silicon alleviates nonalcoholic steatohepatitis by reducing apoptosis in aged Wistar rats fed a high-saturated fat, high-cholesterol diet","volume":"147","author":"Garcimartin","year":"2017","journal-title":"J. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1089\/jmf.2016.0161","article-title":"Chia oil\u2013enriched restructured pork effects on oxidative and inflammatory status of aged rats fed high cholesterol\/high fat diets","volume":"20","author":"Bastida","year":"2017","journal-title":"J. Med. Food"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1081\/DCT-200039714","article-title":"The toxicity profile of hydrolyzed aqueous olive pulp extract","volume":"27","author":"Christian","year":"2004","journal-title":"Drug Chem. Toxicol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6107","DOI":"10.1016\/j.biortech.2009.06.059","article-title":"Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities","volume":"100","author":"Lee","year":"2009","journal-title":"Bioresour. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1007\/s11130-010-0179-z","article-title":"Wakame and Nori in restructured meats included in cholesterol-enriched diets affect the antioxidant enzyme gene expressions and activities in Wistar rats","volume":"65","author":"Schultz","year":"2010","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1093\/jn\/123.11.1939","article-title":"AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet","volume":"123","author":"Reeves","year":"1993","journal-title":"J. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1017\/S0007114510005015","article-title":"Effects of hydroxytyrosol-enriched sunflower oil consumption on CVD risk factors","volume":"105","author":"Lucas","year":"2011","journal-title":"Br. J. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/0305-0491(82)90479-5","article-title":"The density profile and cholesterol concentration of serum lipoproteins in domestic and laboratory animals","volume":"71","author":"Terpstra","year":"1982","journal-title":"Comp. Biochem. Physiol. B"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1017\/S000711451100198X","article-title":"Effects of Nori-and Wakame-enriched meats with or without supplementary cholesterol on arylesterase activity, lipaemia and lipoproteinaemia in growing Wistar rats","volume":"106","author":"Bastida","year":"2011","journal-title":"Br. J. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1017\/S000711450999105X","article-title":"Effect of seaweed and cholesterol-enriched diets on postprandial lipoproteinaemia in rats","volume":"102","author":"Bocanegra","year":"2009","journal-title":"Br. J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212 \u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ejphar.2013.03.052","article-title":"Age-dependent modulation of central ghrelin effects on food intake and lipid metabolism in rats","volume":"710","author":"Nesic","year":"2013","journal-title":"Eur. J. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.cger.2007.06.001","article-title":"The anorexia of aging","volume":"23","author":"Chapman","year":"2007","journal-title":"Clin. Geriatr. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1093\/jn\/114.9.1640","article-title":"Cholesterol metabolism in two strains of rats with high or low response of serum cholesterol to a cholesterol-rich diet","volume":"114","author":"Beynen","year":"1984","journal-title":"J. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1159\/000343722","article-title":"Effects of restructured pork containing Himanthalia elongata on adipose tissue lipogenic and lipolytic enzyme expression of normo- and hypercholesterolemic rats","volume":"5","author":"Churruca","year":"2012","journal-title":"Lifestyle Genom."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0009-8981(97)00106-X","article-title":"Lipid peroxidation and antioxidant status in experimental animals: Effects of aging and hypercholesterolemic diet","volume":"265","author":"Seven","year":"1997","journal-title":"Clin. Chim. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/ejlt.200700280","article-title":"Do not use the Friedewald formula to calculate LDL-cholesterol in hypercholesterolaemic rats","volume":"110","author":"Bastida","year":"2008","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/BF01612780","article-title":"Olive oil-and sunflower oil-fried sardines in the prevention of rat hypercholesterolemia","volume":"34","author":"Cava","year":"1995","journal-title":"Z. Ern\u00e4hrungswiss"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.atherosclerosis.2007.11.008","article-title":"Omega-3 fatty acids and coronary heart disease risk: Clinical and mechanistic perspectives","volume":"197","author":"Harris","year":"2008","journal-title":"Atherosclerosis"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s11130-016-0543-8","article-title":"Chia seed shows good protein quality, hypoglycemic effect and improves the lipid profile and liver and intestinal morphology of Wistar rats","volume":"71","year":"2016","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"971890","DOI":"10.1155\/2014\/971890","article-title":"Olive leaf extract attenuates obesity in high-fat diet-fed mice by modulating the expression of molecules involved in adipogenesis and thermogenesis","volume":"2014","author":"Shen","year":"2014","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1002\/jcb.20951","article-title":"Modified HMG-CoA reductase and LDLr regulation is deeply involved in age-related hypercholesterolemia","volume":"98","author":"Pallottini","year":"2006","journal-title":"J. Cell. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"444S","DOI":"10.1093\/jn\/128.2.444S","article-title":"Dietary fatty acids and the regulation of plasma low density lipoprotein cholesterol concentrations","volume":"128","author":"Dietschy","year":"1998","journal-title":"J. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1177\/1082013203038860","article-title":"Effect of olive oil-fried sardine consumption on liver lipid composition and fatty acid cholesterol esterification in hypercholesterolemic rats","volume":"9","author":"Viejo","year":"2003","journal-title":"Food Sci. Technol. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1002\/ejlt.201300147","article-title":"Lipoproteinemia and arylesterase activity in Zucker fa\/fa rats fed glucomannan\/spirulina-enriched squid-surimi","volume":"115","author":"Bastida","year":"2013","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1093\/jn\/133.7.2302","article-title":"Fat and protein from olive-oil-fried sardines interact to normalize serum lipoproteins and reduce liver lipids in hypercholesterolemic rats","volume":"133","author":"Bastida","year":"2003","journal-title":"J. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0025-7125(16)31426-2","article-title":"Atherogenic hyperlipoproteinemia: The cellular and molecular biology of plasma lipoproteins altered by dietary fat and cholesterol","volume":"66","author":"Mahley","year":"1982","journal-title":"Med. Clin. North Am."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1016\/j.bbalip.2006.09.008","article-title":"Cholesteryl ester transfer protein (CETP) expression enhances HDL cholesteryl ester liver delivery, which is independent of scavenger receptor BI, LDL receptor related protein and possibly LDL receptor","volume":"1761","author":"Zhou","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4141\/A98-048","article-title":"An \u03c9-3 fatty acid enriched chia diet: Influence on egg fatty acid composition, cholesterol and oil content","volume":"79","author":"Ayerza","year":"1999","journal-title":"Can. J. Anim. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jnutbio.2010.11.011","article-title":"Lipid redistribution by \u03b1-linolenic acid-rich chia seed inhibits stearoyl-CoA desaturase-1 and induces cardiac and hepatic protection in diet-induced obese rats","volume":"23","author":"Poudyal","year":"2012","journal-title":"J. Nutr. Biochem."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/10\/12\/1830\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:32:12Z","timestamp":1760196732000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/10\/12\/1830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,26]]},"references-count":48,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["nu10121830"],"URL":"https:\/\/doi.org\/10.3390\/nu10121830","relation":{},"ISSN":["2072-6643"],"issn-type":[{"type":"electronic","value":"2072-6643"}],"subject":[],"published":{"date-parts":[[2018,11,26]]}}}