{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T14:52:21Z","timestamp":1782139941542,"version":"3.54.5"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T00:00:00Z","timestamp":1558915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Severe corneal inflammation produces opacity or even perforation, scarring, and angiogenesis, resulting in blindness. In this study, we used the cornea to examine the effect of new anti-angiogenic chemopreventive agents. We researched the anti-angiogenic effect of two extracts, methanol (Met) and hexane (Hex), from the seed of Cucurbita argyrosperma, on inflamed corneas. The corneas of Wistar rats were alkali-injured and treated intragastrically for seven successive days. We evaluated: opacity score, corneal neovascularization (CNV) area, re-epithelialization percentage, and histological changes. Also, we assessed the inflammatory (cyclooxigenase-2, nuclear factor-kappaB, and interleukin-1\u03b2) and angiogenic (vascular endothelial growth factor A, VEGF-A; -receptor 1, VEGFR1; and -receptor 2, VEGFR2) markers. Levels of Cox-2, Il-1\u03b2, and Vegf-a mRNA were also determined. After treatment, we observed a reduction in corneal edema, with lower opacity scores and cell infiltration compared to untreated rats. Treatment also accelerated wound healing and decreased the CNV area. The staining of inflammatory and angiogenic factors was significantly decreased and related to a down-expression of Cox-2, Il-1\u03b2, and Vegf. These results suggest that intake of C. argyrosperma seed has the potential to attenuate the angiogenesis secondary to inflammation in corneal chemical damage.<\/jats:p>","DOI":"10.3390\/nu11051184","type":"journal-article","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T11:19:27Z","timestamp":1558955967000},"page":"1184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model"],"prefix":"10.3390","volume":"11","author":[{"given":"Mar\u00eda Fernanda","family":"Estrella-Mendoza","sequence":"first","affiliation":[{"name":"Laboratorio de Investigac\u00edon de Productos Naturales, Escuela de Ingenier\u00eda Qu\u00edmica e Industrias Extractivas, Instituto Polit\u00e9cnico Nacional, Unidad Profesional Adolfo L\u00f3pez Mateos, Av. Instituto Polit\u00e9cnico Nacional S\/N, Ciudad de M\u00e9xico 07708, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Jim\u00e9nez-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Investigaci\u00f3n, Hospital Ju\u00e1rez de M\u00e9xico, Av. Instituto Polit\u00e9cnico Nacional 5160, Magdalena de las Salinas, Gustavo A. Madero, Ciudad de M\u00e9xico 07760, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo","family":"L\u00f3pez-Ornelas","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Investigaci\u00f3n, Hospital Ju\u00e1rez de M\u00e9xico, Av. Instituto Polit\u00e9cnico Nacional 5160, Magdalena de las Salinas, Gustavo A. Madero, Ciudad de M\u00e9xico 07760, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa Martha","family":"P\u00e9rez-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigac\u00edon de Productos Naturales, Escuela de Ingenier\u00eda Qu\u00edmica e Industrias Extractivas, Instituto Polit\u00e9cnico Nacional, Unidad Profesional Adolfo L\u00f3pez Mateos, Av. Instituto Polit\u00e9cnico Nacional S\/N, Ciudad de M\u00e9xico 07708, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7029-7036","authenticated-orcid":false,"given":"Javier","family":"Flores-Estrada","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Investigaci\u00f3n, Hospital Ju\u00e1rez de M\u00e9xico, Av. Instituto Polit\u00e9cnico Nacional 5160, Magdalena de las Salinas, Gustavo A. Madero, Ciudad de M\u00e9xico 07760, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,27]]},"reference":[{"key":"ref_1","first-page":"264","article-title":"Corneal angiogenic privilege: Angiogenic and antiangiogenic factors in corneal avascularity, vasculogenesis, and wound healing (an American Ophthalmological Society thesis)","volume":"104","author":"Azar","year":"2006","journal-title":"Trans. Am. Ophthalmol. 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