{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T11:05:08Z","timestamp":1768647908348,"version":"3.49.0"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,6,8]],"date-time":"2019-06-08T00:00:00Z","timestamp":1559952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Economy and Competitivity","award":["SAF2014-53209-R"],"award-info":[{"award-number":["SAF2014-53209-R"]}]},{"name":"RIRAAF Network","award":["RD16\/0006\/0014"],"award-info":[{"award-number":["RD16\/0006\/0014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC\u2013MS and LC\u2013IT\/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NF\u03baB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy.<\/jats:p>","DOI":"10.3390\/nu11061303","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T03:16:51Z","timestamp":1560136611000},"page":"1303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function"],"prefix":"10.3390","volume":"11","author":[{"given":"Sara","family":"Bened\u00e9","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Ciencias Qu\u00edmicas, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Gradillas","sequence":"additional","affiliation":[{"name":"Cembio (Centro de Metabol\u00f3mica y Bioan\u00e1lisis), Departamento de Qu\u00edmica y Bioqu\u00edmica, Facultad de Farmacia, Universidad CEU San Pablo, 28668 Monteprincipe, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayte","family":"Villalba","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Ciencias Qu\u00edmicas, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Batanero","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Ciencias Qu\u00edmicas, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,8]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (1999). 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