{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T11:19:46Z","timestamp":1772018386187,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,8,25]],"date-time":"2019-08-25T00:00:00Z","timestamp":1566691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG Research Unit 2558 TraceAge, Scho 849\/6-1"],"award-info":[{"award-number":["DFG Research Unit 2558 TraceAge, Scho 849\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["STO 1099\/-2 to CS"],"award-info":[{"award-number":["STO 1099\/-2 to CS"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Selenium-binding protein 1 (SELENBP1) is an intracellular protein that has been detected in the circulation in response to myocardial infarction. Hypoxia and cardiac surgery affect selenoprotein expression and selenium (Se) status. For this reason, we decided to analyze circulating SELENBP1 concentrations in patients (n = 75) necessitating cardioplegia and a cardiopulmonary bypass (CPB) during the course of the cardiac surgery. Serum samples were collected at seven time-points spanning the full surgical process. SELENBP1 was quantified by a highly sensitive newly developed immunological assay. Serum concentrations of SELENBP1 increased markedly during the intervention and showed a positive association with the duration of ischemia (\u03c1 = 0.6, p &lt; 0.0001). Elevated serum SELENBP1 concentrations at 1 h after arrival at the intensive care unit (post-surgery) were predictive to identify patients at risk of adverse outcome (death, bradycardia or cerebral ischemia, \u201cendpoint 1\u201d; OR 29.9, CI 3.3\u2013268.8, p = 0.00027). Circulating SELENBP1 during intervention (2 min after reperfusion or 15 min after weaning from the CPB) correlated positively with an established marker of myocardial infarction (CK-MB) measured after the intervention (each with \u03c1 = 0.5, p &lt; 0.0001). We concluded that serum concentrations of SELENBP1 were strongly associated with cardiac arrest and the duration of myocardial ischemia already early during surgery, thereby constituting a novel and promising quantitative marker for myocardial hypoxia, with a high potential to improve diagnostics and prediction in combination with the established clinical parameters.<\/jats:p>","DOI":"10.3390\/nu11092005","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T04:38:23Z","timestamp":1566794303000},"page":"2005","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Selenium-Binding Protein 1 Indicates Myocardial Stress and Risk for Adverse Outcome in Cardiac Surgery"],"prefix":"10.3390","volume":"11","author":[{"given":"Ellen C. D.","family":"K\u00fchn-Heid","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Experimentelle Endokrinologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, D-13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0644-1715","authenticated-orcid":false,"given":"Eike C.","family":"K\u00fchn","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Endokrinologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, D-13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Ney","sequence":"additional","affiliation":[{"name":"Cardiovascular Critical Care &amp; Anesthesia Research and Evaluation (3CARE), RWTH-Aachen University, D-52074 Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Wendt","sequence":"additional","affiliation":[{"name":"Cardiovascular Critical Care &amp; Anesthesia Research and Evaluation (3CARE), RWTH-Aachen University, D-52074 Aachen, Germany"},{"name":"Department of Anesthesiology, Uniklinik RWTH-Aachen, D-52074 Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6081-2316","authenticated-orcid":false,"given":"Julian","family":"Seelig","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Endokrinologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, D-13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Schwiebert","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Endokrinologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, D-13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waldemar B.","family":"Minich","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Endokrinologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, D-13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-2039","authenticated-orcid":false,"given":"Christian","family":"Stoppe","sequence":"additional","affiliation":[{"name":"Cardiovascular Critical Care &amp; Anesthesia Research and Evaluation (3CARE), RWTH-Aachen University, D-52074 Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9445-1555","authenticated-orcid":false,"given":"Lutz","family":"Schomburg","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Endokrinologie, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, D-13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1089\/ars.2012.5013","article-title":"Selenocysteine in thiol\/disulfide-like exchange reactions","volume":"18","author":"Hondal","year":"2013","journal-title":"Antioxid. Redox Signal."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.abb.2016.08.003","article-title":"Selenium and redox signaling","volume":"617","year":"2017","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.3390\/nu7053621","article-title":"Selenium and chronic diseases: A nutritional genomics perspective","volume":"7","year":"2015","journal-title":"Nutrients"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1146\/annurev-nutr-071714-034250","article-title":"Regulation of Selenium Metabolism and Transport","volume":"35","author":"Burk","year":"2015","journal-title":"Ann. Rev. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1016\/j.bbagen.2009.03.015","article-title":"Hierarchical regulation of selenoprotein expression and sex-specific effects of selenium","volume":"1790","author":"Schomburg","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1515\/BC.2007.128","article-title":"Effect of age on sexually dimorphic selenoprotein expression in mice","volume":"388","author":"Schomburg","year":"2007","journal-title":"Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1017\/S0007114508939830","article-title":"Food-chain selenium and human health: Emphasis on intake","volume":"100","author":"Rayman","year":"2008","journal-title":"Br. J. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1152\/physrev.00039.2013","article-title":"Selenoproteins: Molecular pathways and physiological roles","volume":"94","author":"Labunskyy","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1093\/carcin\/10.3.541","article-title":"Evidence for two selenium-binding proteins distinct from glutathione peroxidase in mouse liver","volume":"10","author":"Bansal","year":"1989","journal-title":"Carcinogenesis"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Elhodaky, M., and Diamond, A.M. (2018). Selenium-Binding Protein 1 in Human Health and Disease. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113437"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/(SICI)1097-4644(199702)64:2<217::AID-JCB5>3.0.CO;2-#","article-title":"Isolation, characterization, and chromosomal mapping of a novel cDNA clone encoding human selenium binding protein","volume":"64","author":"Chang","year":"1997","journal-title":"J. Cell Biochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1093\/carcin\/14.3.335","article-title":"Different patterns of regulation of the genes encoding the closely related 56 kDa selenium- and acetaminophen-binding proteins in normal tissues and during carcinogenesis","volume":"14","author":"Lanfear","year":"1993","journal-title":"Carcinogenesis"},{"key":"ref_13","first-page":"3150","article-title":"Differential expression and androgen regulation of the human selenium-binding protein gene hSP56 in prostate cancer cells","volume":"58","author":"Yang","year":"1998","journal-title":"Cancer Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1093\/carcin\/bgq114","article-title":"Functional and physical interaction between the selenium-binding protein 1 (SBP1) and the glutathione peroxidase 1 selenoprotein","volume":"31","author":"Fang","year":"2010","journal-title":"Carcinogenesis"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1016\/j.humpath.2011.01.008","article-title":"Suppression of selenium-binding protein 1 in gastric cancer is associated with poor survival","volume":"42","author":"Xia","year":"2011","journal-title":"Hum. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zhang, S., Li, F., Younes, M., Liu, H., Chen, C., and Yao, Q. (2013). Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0063702"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1186\/1477-7819-12-288","article-title":"Decreased selenium-binding protein 1 mRNA expression is associated with poor prognosis in renal cell carcinoma","volume":"12","author":"Ha","year":"2014","journal-title":"World J. Surg. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1002\/biof.5520140115","article-title":"Plasma selenium in specific and non-specific forms","volume":"14","author":"Burk","year":"2001","journal-title":"BioFactors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"26839","DOI":"10.1074\/jbc.M401907200","article-title":"Determinants of human plasma glutathione peroxidase (GPx-3) expression","volume":"279","author":"Bierl","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"G32","DOI":"10.1152\/ajpgi.00064.2011","article-title":"Glutathione peroxidase-3 produced by the kidney binds to a population of basement membranes in the gastrointestinal tract and in other tissues","volume":"301","author":"Burk","year":"2011","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1042\/bj20021853","article-title":"Gene disruption discloses role of selenoprotein P in selenium delivery to target tissues","volume":"370","author":"Schomburg","year":"2003","journal-title":"Biochem. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13640","DOI":"10.1074\/jbc.M300755200","article-title":"Deletion of selenoprotein P alters distribution of selenium in the mouse","volume":"278","author":"Hill","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jtemb.2007.11.003","article-title":"New assay for the measurement of selenoprotein P as a sepsis biomarker from serum","volume":"22","author":"Hollenbach","year":"2008","journal-title":"J. Trace Elem. Med. Biol. Organ Soc. Miner. Trace Elem. (GMS)"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1007\/s00018-016-2339-2","article-title":"Selenoproteins and oxidative stress-induced inflammatory tumorigenesis in the gut","volume":"74","author":"Barrett","year":"2017","journal-title":"Cell. Mol. Life Sci. CMLS"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Polyzos, S.A., Kountouras, J., Mavrouli, M., Katsinelos, P., Doulberis, M., Gavana, E., and Duntas, L. (2019). Selenoprotein P in Patients with Nonalcoholic Fatty Liver Disease. Exp. Clin. Endocrinol. Diabetes Off. J. Ger. Soc. Endocrinol. Ger. Diabetes Assoc.","DOI":"10.1055\/a-0811-9136"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Meyer, H.A., Endermann, T., Stephan, C., Stoedter, M., Behrends, T., Wolff, I., Jung, K., and Schomburg, L. (2012). Selenoprotein P status correlates to cancer-specific mortality in renal cancer patients. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046644"},{"key":"ref_27","first-page":"338","article-title":"Selenoprotein P, rather than glutathione peroxidase, as a potential marker of septic shock and related syndromes","volume":"43","author":"Forceville","year":"2009","journal-title":"Eur. Surg. Res. Eur. Chir. Forsch. Rech. Chir. Eur."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Braunstein, M., Kusmenkov, T., Zuck, C., Angstwurm, M., Becker, N.P., Bocker, W., Schomburg, L., and Bogner-Flatz, V. (2019). Selenium and Selenoprotein P Deficiency Correlates with Complications and Adverse Outcome After Major Trauma. Shock (AugustaGa).","DOI":"10.1097\/SHK.0000000000001344"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1080\/15287394.2017.1299655","article-title":"Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury","volume":"80","author":"Kim","year":"2017","journal-title":"J. Toxicol. Environ. Health Part A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.jtemb.2019.01.005","article-title":"Circulating levels of selenium-binding protein 1 (SELENBP1) are associated with risk for major adverse cardiac events and death","volume":"52","author":"Slagman","year":"2019","journal-title":"J. Trace Elem. Med. Biol. Organ Soc. Miner. Trace Elem. (GMS)"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0021-9150(92)90035-F","article-title":"Serum selenium concentration and risk of ischaemic heart disease in a prospective cohort study of 3000 males","volume":"96","author":"Suadicani","year":"1992","journal-title":"Atherosclerosis"},{"key":"ref_32","first-page":"528","article-title":"Selenium in Cardiac Surgery","volume":"34","author":"Wendt","year":"2019","journal-title":"Nutr. Clin. Pract. Off. Publ. Am. Soc. Parenter. Enter. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S1010-7940(99)00134-7","article-title":"European system for cardiac operative risk evaluation (EuroSCORE)","volume":"16","author":"Nashef","year":"1999","journal-title":"Eur. J. Cardio Thorac. Surg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"27599","DOI":"10.3390\/ijms161126043","article-title":"A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1","volume":"16","author":"Ying","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.1242\/jeb.01109","article-title":"A unique pathway of cardiac myocyte death caused by hypoxia-acidosis","volume":"207","author":"Graham","year":"2004","journal-title":"J. Exp. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1007\/s00204-016-1832-6","article-title":"Selenium-binding protein 1: A sensitive urinary biomarker to detect heavy metal-induced nephrotoxicity","volume":"91","author":"Lee","year":"2017","journal-title":"Arch. Toxicol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1556\/APhysiol.95.2008.2.3","article-title":"Does selenium exert cardioprotective effects against oxidative stress in myocardial ischemia?","volume":"95","author":"Boucher","year":"2008","journal-title":"Acta Physiol. Hung."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Alehagen, U., Aaseth, J., and Johansson, P. (2015). Reduced Cardiovascular Mortality 10 Years after Supplementation with Selenium and Coenzyme Q10 for Four Years: Follow-Up Results of a Prospective Randomized Double-Blind Placebo-Controlled Trial in Elderly Citizens. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0141641"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1001\/jama.2012.199","article-title":"Association of age and sex with myocardial infarction symptom presentation and in-hospital mortality","volume":"307","author":"Canto","year":"2012","journal-title":"JAMA"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/11\/9\/2005\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:13:51Z","timestamp":1760188431000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/11\/9\/2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,25]]},"references-count":39,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["nu11092005"],"URL":"https:\/\/doi.org\/10.3390\/nu11092005","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,25]]}}}