{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:07:37Z","timestamp":1791644857505,"version":"4.3.4"},"reference-count":85,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T00:00:00Z","timestamp":1567382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Scarce data exist on the body composition of lean women with polycystic ovary syndrome (PCOS) on treatment with metformin and oral contraceptives (OCs). Thirty-four lean (body mass index 18.5\u201324.9 kg\/m2) women (17 with PCOS on metformin and OCs treatment for six months and 17 controls) aged 18\u201340 years were assessed for body composition parameters (fat, muscle, glycogen, protein masses, bone masses, and body water compartments) and phase angles. PCOS patients demonstrated lower muscle, glycogen and protein masses (U = 60, p = 0.003), along with a lower bone mineral content and mass (U = 78, p = 0.021; U = 74, p = 0.014) than their healthy counterparts, while total and abdominal fat masses were similar between the two groups. PCOS patients also exhibited increased extracellular body water (U = 10, p &lt; 0.001) and decreased intracellular water, compatible with low-grade inflammation and cellular dehydration. Key differences in body composition between women with PCOS and controls demonstrated an osteosarcopenic body composition phenotype in PCOS patients. A confirmation of these findings in larger studies may render osteosarcopenia management a targeted adjunct therapy in women with PCOS.<\/jats:p>","DOI":"10.3390\/nu11092055","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T03:06:14Z","timestamp":1567479974000},"page":"2055","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Lean Women on Metformin and Oral Contraceptives for Polycystic Ovary Syndrome Demonstrate a Dehydrated Osteosarcopenic Phenotype: A Pilot Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1634-701X","authenticated-orcid":false,"given":"Charikleia","family":"Stefanaki","sequence":"first","affiliation":[{"name":"Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, First Department of Pediatrics, School of Medicine, National and Kapodistrian University of Athens, Aghia Sophia Children\u2019s Hospital, 11527 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1001-0926","authenticated-orcid":false,"given":"Flora","family":"Bacopoulou","sequence":"additional","affiliation":[{"name":"Center for Adolescent Medicine and UNESCO Chair on Adolescent Health Care, First Department of Pediatrics, School of Medicine, National and Kapodistrian University of Athens, Aghia Sophia Children\u2019s Hospital, 11527 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-5369","authenticated-orcid":false,"given":"Eleni","family":"Kandaraki","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism, Hygeia Hospital, 15123 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Boschiero","sequence":"additional","affiliation":[{"name":"BIOTEKNA Biomedical Technologies, 30020 Venice, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evanthia","family":"Diamandi-Kandarakis","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism, Hygeia Hospital, 15123 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.fertnstert.2011.09.024","article-title":"Consensus on women\u2019s health aspects of polycystic ovary syndrome (PCOS): The Amsterdam ESHRE\/ASRM-Sponsored 3rd PCOS Consensus Workshop Group","volume":"97","author":"Fauser","year":"2012","journal-title":"Fertil. Steril."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4006","DOI":"10.1210\/jcem.84.11.6148","article-title":"A survey of the polycystic ovary syndrome in the Greek island of Lesbos: Hormonal and metabolic profile","volume":"84","author":"Kouli","year":"1999","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1017\/S0954422416000287","article-title":"Metabolic consequences of obesity and insulin resistance in polycystic ovary syndrome: Diagnostic and methodological challenges","volume":"30","author":"Jeanes","year":"2017","journal-title":"Nutr. Res. Rev."},{"key":"ref_4","first-page":"669","article-title":"Differences in the psychological and hormonal presentation of lean and obese patients with polycystic ovary syndrome","volume":"34","author":"Komarowska","year":"2013","journal-title":"Neuro Endocrinol. Lett."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.1210\/jc.2013-2350","article-title":"Diagnosis and treatment of polycystic ovary syndrome: An Endocrine Society clinical practice guideline","volume":"98","author":"Legro","year":"2013","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s40200-018-0371-5","article-title":"Lean polycystic ovary syndrome (PCOS): An evidence-based practical approach","volume":"17","author":"Toosy","year":"2018","journal-title":"J. Diabetes Metab. Disord."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"154","DOI":"10.4103\/jhrs.JHRS_77_17","article-title":"Debates Regarding Lean Patients with Polycystic Ovary Syndrome: A Narrative Review","volume":"10","author":"Goyal","year":"2017","journal-title":"J. Hum. Reprod. Sci."},{"key":"ref_8","unstructured":"Health, N.I.O. (2015, May 15). Bioelectrical Impedance Analysis in Body Composition Measurement, Available online: http:\/\/consensus.nih.gov\/1994\/1994BioelectricImpedanceBodyta015html.htm."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1093\/ajcn\/80.6.1634","article-title":"Bioelectrical impedance phase angle in clinical practice: Implications for prognosis in advanced colorectal cancer","volume":"80","author":"Gupta","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_10","first-page":"181","article-title":"Techniques for the diagnosis of sarcopenia","volume":"11","author":"Rubbieri","year":"2014","journal-title":"Clin. Cases Miner. Bone Metab. Off. J. Ital. Soc. Osteoporos. Miner. Metab. Skelet. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1038\/sj.ijo.0803475","article-title":"Validity of the leg-to-leg bioimpedance to estimate changes in body fat during weight loss and regain in overweight women: A comparison with multi-compartment models","volume":"31","author":"Jebb","year":"2007","journal-title":"Int. J. Obes."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4103\/jhrs.JHRS_78_17","article-title":"Possible Link Between Stress-related Factors and Altered Body Composition in Women with Polycystic Ovarian Syndrome","volume":"11","author":"Basu","year":"2018","journal-title":"J. Hum. Reprod. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s42000-018-0023-7","article-title":"Chronic stress and body composition disorders: Implications for health and disease","volume":"17","author":"Stefanaki","year":"2018","journal-title":"Hormones"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1161\/CIRCRESAHA.116.308445","article-title":"Anti-Inflammatory Effects of Metformin Irrespective of Diabetes Status","volume":"119","author":"Cameron","year":"2016","journal-title":"Circ. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/toxsci\/kfw158","article-title":"Editor\u2019s Highlight: Metformin Protects Against Acetaminophen Hepatotoxicity by Attenuation of Mitochondrial Oxidant Stress and Dysfunction","volume":"154","author":"Du","year":"2016","journal-title":"Toxicol. Sci. Off. J. Soc. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12937-016-0186-5","article-title":"The importance of antioxidants which play the role in cellular response against oxidative\/nitrosative stress: Current state","volume":"15","author":"Kurutas","year":"2016","journal-title":"Nutr. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4692197","DOI":"10.1155\/2018\/4692197","article-title":"Comparative Biochemical and Histopathological Studies on the Efficacy of Metformin and Virgin Olive Oil against Streptozotocin-Induced Diabetes in Sprague-Dawley Rats","volume":"2018","author":"Balamash","year":"2018","journal-title":"J. Diabetes Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9150807","DOI":"10.1155\/2018\/9150807","article-title":"Antioxidant and Hypoglycemic Effects of Acidic-Extractable Polysaccharides from Cordyceps militaris on Type 2 Diabetes Mice","volume":"2018","author":"Zhao","year":"2018","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3109\/10253890.2014.974030","article-title":"Impact of a mindfulness stress management program on stress, anxiety, depression and quality of life in women with polycystic ovary syndrome: A randomized controlled trial","volume":"18","author":"Stefanaki","year":"2015","journal-title":"Stress"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1530\/JOE-13-0175","article-title":"Dietary glycotoxins affect scavenger receptor expression and the hormonal profile of female rats","volume":"218","author":"Chatzigeorgiou","year":"2013","journal-title":"J. Endocrinol."},{"key":"ref_21","first-page":"636","article-title":"Hypothalamic-pituitary-adrenocortical axis hypersensitivity and glucocorticoid receptor expression and function in women with polycystic ovary syndrome","volume":"119","author":"Milutinovic","year":"2011","journal-title":"Exp. Clin. Endocrinol. Diabetes Off. J. Ger. Soc. Endocrinol. Ger. Diabetes Assoc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1111\/j.1365-2265.2008.03247.x","article-title":"Increased serum advanced glycation end-products is a distinct finding in lean women with polycystic ovary syndrome (PCOS)","volume":"69","author":"Katsikis","year":"2008","journal-title":"Clin. Endocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"P1","DOI":"10.1530\/EJE-14-0253","article-title":"The polycystic ovary syndrome: A position statement from the European Society of Endocrinology","volume":"171","author":"Conway","year":"2014","journal-title":"Eur. J. Endocrinol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.1210\/jcem.85.8.6738","article-title":"Effect of long-term treatment with metformin added to hypocaloric diet on body composition, fat distribution, and androgen and insulin levels in abdominally obese women with and without the polycystic ovary syndrome","volume":"85","author":"Pasquali","year":"2000","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s40200-014-0088-z","article-title":"Body composition in adults with newly diagnosed type 2 diabetes: Effects of metformin","volume":"13","author":"Aghili","year":"2014","journal-title":"J. Diabetes Metab. Disord."},{"key":"ref_26","first-page":"725","article-title":"The effect of orlistat versus metformin on body composition and insulin resistance in obese premenopausal women: 3-month randomized prospective open-label study","volume":"13","author":"Musialik","year":"2017","journal-title":"Arch. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.contraception.2012.07.005","article-title":"Body composition in lean women with polycystic ovary syndrome: Effect of ethinyl estradiol and drospirenone combination","volume":"87","author":"Aydin","year":"2013","journal-title":"Contraception"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1093\/humrep\/der070","article-title":"Comparison of MRI-assessed body fat content between lean women with polycystic ovary syndrome (PCOS) and matched controls: Less visceral fat with PCOS","volume":"26","author":"Dolfing","year":"2011","journal-title":"Hum. Reprod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"623","DOI":"10.29252\/ijrm.16.10.623","article-title":"Body fat percentage is a better marker than body mass index for determining inflammation status in polycystic ovary syndrome","volume":"16","author":"Hestiantoro","year":"2018","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1519\/JSC.0000000000002714","article-title":"Hyperandrogenism Enhances Muscle Strength After Progressive Resistance Training, Independent of Body Composition, in Women With Polycystic Ovary Syndrome","volume":"32","author":"Kogure","year":"2018","journal-title":"J. Strength Cond. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ejogrb.2016.06.022","article-title":"Evaluation of hs-CRP and visseral adiposity index in patients with policystic ovary syndrome by clinical and laboratory findings","volume":"204","author":"Un","year":"2016","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/eci.12388","article-title":"Stress and inflammatory biomarkers and symptoms are associated with bioimpedance measures","volume":"45","author":"Tsigos","year":"2015","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_33","first-page":"181","article-title":"Bioimpedance analysis vs. DEXA as a screening tool for osteosarcopenia in lean, overweight and obese Caucasian postmenopausal females","volume":"16","author":"Peppa","year":"2017","journal-title":"Hormones"},{"key":"ref_34","first-page":"65","article-title":"A simple sequential rejective method procedure","volume":"6","author":"Holm","year":"1979","journal-title":"Scand. J. Stat."},{"key":"ref_35","unstructured":"Gaetano, J. (2015, May 12). Dataset-Holm-Bonferroni Sequential Correction: An EXCEL Calculator\u2014Ver. 1.2. Available online: https:\/\/www.researchgate.net\/publication\/242331583_Holm-Bonferroni_Sequential_Correction_An_EXCEL_Calculator_-_Ver._1.2."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/pst.185","article-title":"Sample size of 12 per group rule of thumb for a pilot study","volume":"4","author":"Julious","year":"2005","journal-title":"Pharmaceut. Stat."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.bbrc.2013.09.026","article-title":"Metformin-induced inhibition of the mitochondrial respiratory chain increases FGF21 expression via ATF4 activation","volume":"440","author":"Kim","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_38","first-page":"337","article-title":"Serum androgen levels and muscle mass in women with polycystic ovary syndrome","volume":"94","author":"Douchi","year":"1999","journal-title":"Obstet. Gynecol."},{"key":"ref_39","first-page":"511","article-title":"Lean muscle mass in classic or ovulatory PCOS: Association with central obesity and insulin resistance","volume":"120","author":"Mario","year":"2012","journal-title":"Exp. Clin. Endocrinol. Diabetes Off. J. Ger. Soc. Endocrinol. Ger. Diabetes Assoc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1210\/jc.2017-02068","article-title":"Progesterone Suppression of Luteinizing Hormone Pulse Frequency in Adolescent Girls With Hyperandrogenism: Effects of Metformin","volume":"103","author":"Lundgren","year":"2018","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1038\/oby.2001.81","article-title":"Obesity and sarcopenia after menopause are reversed by sex hormone replacement therapy","volume":"9","author":"Sorensen","year":"2001","journal-title":"Obes. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.metabol.2006.04.002","article-title":"Plasma interleukin 6 levels are elevated in polycystic ovary syndrome independently of obesity or sleep apnea","volume":"55","author":"Vgontzas","year":"2006","journal-title":"Metab. Clin. Exp."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1016\/j.metabol.2013.07.004","article-title":"Inflammatory cytokines and chemokines, skeletal muscle and polycystic ovary syndrome: Effects of pioglitazone and metformin treatment","volume":"62","author":"Ciaraldi","year":"2013","journal-title":"Metab. Clin. Exp."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.maturitas.2012.07.015","article-title":"Factors contributing to the variability in muscle ageing","volume":"73","author":"Degens","year":"2012","journal-title":"Maturitas"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00424-014-1499-x","article-title":"Exercise-induced AMPK and pyruvate dehydrogenase regulation is maintained during short-term low-grade inflammation","volume":"467","author":"Bienso","year":"2015","journal-title":"Pflug. Arch. Eur. J. Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"295478","DOI":"10.1155\/2014\/295478","article-title":"The acute effects of low-dose TNF-alpha on glucose metabolism and beta-cell function in humans","volume":"2014","author":"Ibfelt","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1111\/eci.12659","article-title":"Healthy overweight\/obese youth: Early osteosarcopenic obesity features","volume":"46","author":"Stefanaki","year":"2016","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_48","first-page":"422","article-title":"Mitochondrial Quality Control and Muscle Mass Maintenance","volume":"6","author":"Romanello","year":"2015","journal-title":"Front. Physiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Carreau, A.M., Pyle, L., Garcia-Reyes, Y., Rahat, H., Vigers, T., Jensen, T., Scherzinger, A., Nadeau, K.J., and Cree-Green, M. (2019). Clinical prediction score of non-alcoholic fatty liver disease in adolescent girls with polycystic ovary syndrome (PCOS-HS index). Clin. Endocrinol.","DOI":"10.1111\/cen.14062"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lonardo, A., Mantovani, A., Lugari, S., and Targher, G. (2019). NAFLD in Some Common Endocrine Diseases: Prevalence, Pathophysiology, and Principles of Diagnosis and Management. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20112841"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1074\/jbc.275.1.223","article-title":"Dimethylbiguanide inhibits cell respiration via an indirect effect targeted on the respiratory chain complex I","volume":"275","author":"Nogueira","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/nature11808","article-title":"Biguanides suppress hepatic glucagon signalling by decreasing production of cyclic AMP","volume":"494","author":"Miller","year":"2013","journal-title":"Nature"},{"key":"ref_53","first-page":"23","article-title":"Diet, metformin and inositol in overweight and obese women with polycystic ovary syndrome: Effects on body composition","volume":"64","author":"Alibrandi","year":"2012","journal-title":"Minerva Ginecol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"465282","DOI":"10.1155\/2012\/465282","article-title":"Metformin stimulates FGF21 expression in primary hepatocytes","volume":"2012","author":"Nygaard","year":"2012","journal-title":"Exp. Diabetes Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1530\/JOE-15-0160","article-title":"FGF21 as a mediator of adaptive responses to stress and metabolic benefits of anti-diabetic drugs","volume":"226","author":"Kim","year":"2015","journal-title":"J. Endocrinol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Wessels, B., Ciapaite, J., van den Broek, N.M., Nicolay, K., and Prompers, J.J. (2014). Metformin impairs mitochondrial function in skeletal muscle of both lean and diabetic rats in a dose-dependent manner. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0100525"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"231","DOI":"10.2174\/1573399810666140918121022","article-title":"Effects of the antidiabetic drugs on the age-related atrophy and sarcopenia associated with diabetes type II","volume":"10","author":"Cetrone","year":"2014","journal-title":"Curr. Diabetes Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"R387","DOI":"10.1152\/ajpregu.00197.2009","article-title":"Low-frequency electroacupuncture and physical exercise decrease high muscle sympathetic nerve activity in polycystic ovary syndrome","volume":"297","author":"Jedel","year":"2009","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1152\/physiol.00041.2007","article-title":"Signaling in muscle atrophy and hypertrophy","volume":"23","author":"Sandri","year":"2008","journal-title":"Physiology"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Banting, L.K., Gibson-Helm, M., Polman, R., Teede, H.J., and Stepto, N.K. (2014). Physical activity and mental health in women with polycystic ovary syndrome. BMC Women Health, 14.","DOI":"10.1186\/1472-6874-14-51"},{"key":"ref_61","first-page":"517","article-title":"Polycystic ovary syndrome is associated with obstructive sleep apnea and daytime sleepiness: Role of insulin resistance","volume":"86","author":"Vgontzas","year":"2001","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s40618-016-0582-x","article-title":"Visceral adiposity index levels in overweight and\/or obese, and non-obese patients with polycystic ovary syndrome and its relationship with metabolic and inflammatory parameters","volume":"40","author":"Durmus","year":"2017","journal-title":"J. Endocrinol. Investig."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"672","DOI":"10.4103\/2230-8210.113759","article-title":"Anthropometric characteristics and dietary pattern of women with polycystic ovary syndrome","volume":"17","author":"Ahmadi","year":"2013","journal-title":"Indian J. Endocrinol. Metab."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2584","DOI":"10.1210\/jc.2014-1135","article-title":"Body composition is improved during 12 months\u2019 treatment with metformin alone or combined with oral contraceptives compared with treatment with oral contraceptives in polycystic ovary syndrome","volume":"99","author":"Glintborg","year":"2014","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.fertnstert.2010.06.058","article-title":"Effect of the insulin sensitizers metformin and pioglitazone on endothelial function in young women with polycystic ovary syndrome: A prospective randomized study","volume":"95","author":"Naka","year":"2011","journal-title":"Fertil. Steril."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1111\/cen.13456","article-title":"Bone markers in polycystic ovary syndrome: A multicentre study","volume":"87","author":"Lingaiah","year":"2017","journal-title":"Clin. Endocrinol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s00223-016-0203-x","article-title":"Diabetes Drug Effects on the Skeleton","volume":"100","author":"Chandran","year":"2017","journal-title":"Calcif. Tissue Int."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1016\/j.pharep.2017.05.008","article-title":"Effect of anti-diabetic drugs on bone metabolism: Evidence from preclinical and clinical studies","volume":"69","author":"Adil","year":"2017","journal-title":"Pharmacol. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s00774-010-0211-2","article-title":"Serum concentrations of carboxylated osteocalcin are increased and associated with several components of the polycystic ovarian syndrome","volume":"29","author":"Livadas","year":"2011","journal-title":"J. Bone Miner. Metab."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.jpag.2011.12.073","article-title":"A comparison of bone mineral density in normal weight and obese adolescents with polycystic ovary syndrome","volume":"25","author":"To","year":"2012","journal-title":"J. Pediatr. Adolesc. Gynecol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Lingaiah, S., Morin-Papunen, L., Risteli, J., and Tapanainen, J.S. (2019). Metformin decreases bone turnover markers in polycystic ovary syndrome: A post hoc study. Fertil. Steril.","DOI":"10.1016\/j.fertnstert.2019.04.013"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1016\/j.fertnstert.2009.01.056","article-title":"Serologic markers of autoimmunity in women with polycystic ovary syndrome","volume":"93","author":"Walch","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"982","DOI":"10.4103\/2230-8210.103006","article-title":"Association of polycystic ovary syndrome and Graves\u2019 disease: Is autoimmunity the link between the two diseases","volume":"16","author":"Nisar","year":"2012","journal-title":"Indian J. Endocrinol. Metab."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/j.autrev.2013.07.003","article-title":"The role of miRNA in inflammation and autoimmunity","volume":"12","author":"Singh","year":"2013","journal-title":"Autoimmun. Rev."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Xu, X., Du, C., Zheng, Q., Peng, L., and Sun, Y. (2014). Effect of metformin on serum interleukin-6 levels in polycystic ovary syndrome: A systematic review. BMC Women Health, 14.","DOI":"10.1186\/1472-6874-14-93"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5775","DOI":"10.2174\/1381612811319320012","article-title":"Mediators of low-grade chronic inflammation in polycystic ovary syndrome (PCOS)","volume":"19","author":"Murri","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1080\/10739680600618918","article-title":"Effect of inflammation on the aging microcirculation: Impact on skeletal muscle blood flow control","volume":"13","author":"Payne","year":"2006","journal-title":"Microcirculation"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1152\/japplphysiol.01032.2003","article-title":"Effects of estrogen and progesterone administration on extracellular fluid","volume":"96","author":"Stachenfeld","year":"2004","journal-title":"J. Appl. Physiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.contraception.2005.09.014","article-title":"Effects of two different oral contraceptives on total body water: A randomized study","volume":"73","author":"Machado","year":"2006","journal-title":"Contraception"},{"key":"ref_80","first-page":"69","article-title":"Evaluation of body composition during low-dose estrogen oral contraceptives treatment","volume":"26","author":"Franchini","year":"1995","journal-title":"Acta Eur. Fertil."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1475-2891-7-21","article-title":"Effect of the ethinylestradiol\/norelgestromin contraceptive patch on body composition. Results of bioelectrical impedance analysis in a population of Italian women","volume":"7","author":"Piccoli","year":"2008","journal-title":"Nutr. J."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.nut.2013.08.017","article-title":"Longitudinal changes in bioimpedance phase angle reflect inverse changes in serum IL-6 levels in maintenance hemodialysis patients","volume":"30","author":"Beberashvili","year":"2014","journal-title":"Nutrition"},{"key":"ref_83","first-page":"711","article-title":"About the benefits and limitations of pilot studies","volume":"81","author":"Dworschak","year":"2015","journal-title":"Minerva Anestesiol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Pin, F., Bonetto, A., Bonewald, L.F., and Klein, G.L. (2019). Molecular Mechanisms Responsible for the Rescue Effects of Pamidronate on Muscle Atrophy in Pediatric Burn Patients. Front. Endocrinol., 10.","DOI":"10.3389\/fendo.2019.00543"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Han, Y., Li, Y., and He, B. (2019). GLP-1 receptor agonists versus metformin in PCOS: A systematic review and meta-analysis. Reprod. Biomed. Online.","DOI":"10.1016\/j.rbmo.2019.04.017"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/11\/9\/2055\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:16:03Z","timestamp":1760188563000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/11\/9\/2055"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,2]]},"references-count":85,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["nu11092055"],"URL":"https:\/\/doi.org\/10.3390\/nu11092055","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,2]]}}}