{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T22:31:27Z","timestamp":1768775487999,"version":"3.49.0"},"reference-count":42,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T00:00:00Z","timestamp":1567555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"Narodowym Centrum Nauki","doi-asserted-by":"publisher","award":["KNOW Consortium \"Healthy Animal-Safe Food\", Ministry of Science and Higher Education, Decision, no 05-1\/KNOW2\/2015"],"award-info":[{"award-number":["KNOW Consortium \"Healthy Animal-Safe Food\", Ministry of Science and Higher Education, Decision, no 05-1\/KNOW2\/2015"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The mechanism of food allergy may vary. This study aimed to compare the effects of milk, yogurt, or beef meat supplementation on humoral and cellular immune responses in a mice model. Mice were divided into four groups: The \u201cMilk group\u201d was sensitized with a \u03b2-lactoglobulin (\u03b2-lg)\/\u03b1-casein (\u03b1-CN) mixture and supplemented cow milk; the \u201cYogurt group\u201d was sensitized with \u03b2-lg\/\u03b1-CN and supplemented yogurt; the \u201cBeef group\u201d was immunized with bovine serum albumin (BSA) and supplemented beef meat; and the \u201cPBS group\u201d received PBS in all procedures. ELISA was used to measure humoral response, including: Total IgE, specific IgG, and IgA. Cellular response was determined by phenotyping lymphocyte from lymphoid tissue and measuring the Th1\/Th2 cytokine concentration with flow cytometry. The qPCR method was used for quantification of the fecal microbiota. The results obtained revealed a lower IgE level for the Yogurt group than for the Milk one. In the Yogurt group, the contribution of regulatory T cells to MLN and PP was higher compared to the other groups. We confirmed that diet supplementation with yogurt modulates the immune response to the prime allergen, and changes the activity of serum antibodies to milk proteins and BSA. Based on a specific antibodies level, we cannot exclude the possibility of CMA mice reaction against BSA.<\/jats:p>","DOI":"10.3390\/nu11092095","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T03:22:36Z","timestamp":1567653756000},"page":"2095","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Milk and Meat Allergens from Bos taurus \u03b2-Lactoglobulin, \u03b1-Casein, and Bovine Serum Albumin: An In-Vivo Study of the Immune Response in Mice"],"prefix":"10.3390","volume":"11","author":[{"given":"Ewa","family":"Fuc","sequence":"first","affiliation":[{"name":"Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10 Str., 10-748 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5624-5707","authenticated-orcid":false,"given":"Dagmara","family":"Z\u0142otkowska","sequence":"additional","affiliation":[{"name":"Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10 Str., 10-748 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Wr\u00f3blewska","sequence":"additional","affiliation":[{"name":"Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10 Str., 10-748 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2332\/allergolint.09-RAI-0138","article-title":"Food Allergy: Review, classification and diagnosis","volume":"58","author":"Cianferoni","year":"2009","journal-title":"Allergol. 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