{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:53:26Z","timestamp":1790744006761,"version":"4.1.0"},"reference-count":85,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T00:00:00Z","timestamp":1568246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Male obesity, which often co-presents with micronutrient deficiencies, is associated with sub-fertility. Here we investigate whether short-term dietary supplementation of micronutrients (zinc, selenium, lycopene, vitamins E and C, folic acid, and green tea extract) to obese mice for 12 days (designed to span the epididymal transit) could improve sperm quality and fetal outcomes. Five-week-old C57BL6 males were fed a control diet (CD, n = 24) or high fat diet (HFD, n = 24) for 10 weeks before allocation to the 12-day intervention of maintaining their original diets (CD, n = 12, HFD n = 12) or with micronutrient supplementation (CD + S, n = 12, HFD + S, n = 12). Measures of sperm quality (motility, morphology, capacitation, binding), sperm oxidative stress (DCFDA, MSR, and 8OHdG), early embryo development (2-cell cleavage, 8OHdG), and fetal outcomes were assessed. HFD + S males had reduced sperm intracellular reactive oxygen species (ROS) concentrations and 8OHdG lesions, which resulted in reduced 8OHdG lesions in the male pronucleus, increased 2-cell cleavage rates, and partial restoration of fetal weight similar to controls. Sub-fertility associated with male obesity may be restored with very short-term micronutrient supplementation that targets the timing of the transit of sperm through the epididymis, which is the developmental window where sperm are the most susceptible to oxidative damage.<\/jats:p>","DOI":"10.3390\/nu11092196","type":"journal-article","created":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T10:56:06Z","timestamp":1568285766000},"page":"2196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Dietary Micronutrient Supplementation for 12 Days in Obese Male Mice Restores Sperm Oxidative Stress"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3492-9403","authenticated-orcid":false,"given":"Nicole O.","family":"McPherson","sequence":"first","affiliation":[{"name":"Robinson Research Institute, School of Medicine, University of Adelaide, Adelaide 5005, Australia"},{"name":"Freemasons Foundation Centre for Men\u2019s Health, University of Adelaide, Adelaide 5005, Australia"},{"name":"Repromed, Dulwich 5065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helana","family":"Shehadeh","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Medicine, University of Adelaide, Adelaide 5005, Australia"},{"name":"Repromed, Dulwich 5065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tod","family":"Fullston","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Medicine, University of Adelaide, Adelaide 5005, Australia"},{"name":"Repromed, Dulwich 5065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deirdre L.","family":"Zander-Fox","sequence":"additional","affiliation":[{"name":"Repromed, Dulwich 5065, Australia"},{"name":"Monash IVF Group, Melbourne 3000, Australia"},{"name":"Future Industries Institute, University of South Australia, Adelaide 5095, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Lane","sequence":"additional","affiliation":[{"name":"Robinson Research Institute, School of Medicine, University of Adelaide, Adelaide 5005, Australia"},{"name":"Monash IVF Group, Melbourne 3000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,12]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2015). 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