{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:36:00Z","timestamp":1780720560663,"version":"3.54.1"},"reference-count":54,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T00:00:00Z","timestamp":1572912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Education, University and Research (MIUR)","award":["RBFR12QW4I_004"],"award-info":[{"award-number":["RBFR12QW4I_004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>To assess the effect of 4 weeks of high fat-high fructose feeding on whole body composition, energy balance, specific markers of oxidative stress and inflammation, and insulin sensitivity in the liver of middle-aged rats, rats (1 year) were fed a diet rich in saturated fatty acids and fructose (HFF rats), mimicking the \u201cWestern diet\u201d, and compared with rats of the same age that were fed a low fat diet (LF rats). HFF rats exhibited a significant increase in the gain of body weight, energy, and lipids compared to LF rats. HFF rats also showed hepatic insulin resistance, together with an increase in plasma triglycerides, cholesterol, and tumor necrosis factor alpha. Hepatic lipids, triglycerides and cholesterol were higher in HFF rats, while a significant decrease in Stearoyl-CoA desaturase activity was found in this tissue. A marked increase in the protein amount of complex I, concomitant to a decrease in its contribution to mitochondrial respiration, was found in HFF rats. Lipid peroxidation and Nitro-Tyrosine content, taken as markers of oxidative stress, as well as NADPH oxidase activity, were significantly higher in HFF rats, while the antioxidant enzyme catalase decreased in these rats. Myeloperoxidase activity and lipocalin content increased, while peroxisome proliferator activated receptor gamma decreased in HFF rats. The present results provide evidence that middle-aged rats show susceptibility to a short-term \u201cWestern diet\u201d, exhibiting altered redox homeostasis, insulin resistance, and early mitochondrial alterations in the liver. Therefore, this type of dietary habits should be drastically limited to pursue a \u201chealthy aging\u201d.<\/jats:p>","DOI":"10.3390\/nu11112670","type":"journal-article","created":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T06:47:57Z","timestamp":1572936477000},"page":"2670","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Early Hepatic Oxidative Stress and Mitochondrial Changes Following Western Diet in Middle Aged Rats"],"prefix":"10.3390","volume":"11","author":[{"given":"Arianna","family":"Mazzoli","sequence":"first","affiliation":[{"name":"Department of Biology, Federico II University, Via Cintia,80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6138-6961","authenticated-orcid":false,"given":"Raffaella","family":"Crescenzo","sequence":"additional","affiliation":[{"name":"Department of Biology, Federico II University, Via Cintia,80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5491-9659","authenticated-orcid":false,"given":"Luisa","family":"Cigliano","sequence":"additional","affiliation":[{"name":"Department of Biology, Federico II University, Via Cintia,80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6980-0661","authenticated-orcid":false,"given":"Maria Stefania","family":"Spagnuolo","sequence":"additional","affiliation":[{"name":"Department of Bio-Agrofood Science, Institute for the Animal Production System in Mediterranean Environment, National Research Council Naples (CNR-ISPAAM), 80147 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Cancelliere","sequence":"additional","affiliation":[{"name":"Department of Biology, Federico II University, Via Cintia,80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Gatto","sequence":"additional","affiliation":[{"name":"Department of Biology, Federico II University, Via Cintia,80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Iossa","sequence":"additional","affiliation":[{"name":"Department of Biology, Federico II University, Via Cintia,80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.cmet.2008.06.012","article-title":"Diet and aging","volume":"8","author":"Piper","year":"2008","journal-title":"Cell Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.mad.2010.03.011","article-title":"Nutrition and aging","volume":"131","author":"Cheng","year":"2010","journal-title":"Mech. 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