{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:17:01Z","timestamp":1784035021302,"version":"3.55.0"},"reference-count":90,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T00:00:00Z","timestamp":1575244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/20421-8"],"award-info":[{"award-number":["2012\/20421-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nestl\u00e9 Institute of Health Sciences","award":["RDHS 000054"],"award-info":[{"award-number":["RDHS 000054"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Vitamins B2, B6, B12, and folate are essential for methylation reactions and possibly influence the transport of polyunsaturated fatty acids in plasma and red blood cells (RBC). Associations between B-vitamin biomarkers and fatty acid (FA) profile were analyzed in Brazilian children and adolescents. This cross-sectional study included 249 children and adolescents, aged 9\u201313 years old. Dietary intake was assessed by the food frequency questionnaire and the healthy eating index (HEI). Biomarkers for vitamins B2, B6, B12, and folate were measured in plasma. The FA profile and the metabolites of one-carbon metabolism were measured in RBC. Associations were tested with multiple linear regression models. An increase of 1 nmol\/L in vitamin B2 was associated with an increase of 0.19 mg\/dL of EPA, 0.20 mg\/dL of ARA, and 0.25 mg\/dL of DHA in RBC. An increase of 1 ng\/mL in plasma folate was associated with an increase of 0.14 mg\/dL of EPA, 0.22 mg\/dL of ARA, and 0.21 mg\/dL of DHA in RBC. These findings highlight the importance of an adequate intake of vitamin B2 and folate in childhood, since they may improve the FA profile in RBCs and may help prevent cardiovascular disease.<\/jats:p>","DOI":"10.3390\/nu11122918","type":"journal-article","created":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T04:58:39Z","timestamp":1575349119000},"page":"2918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Vitamin B2 and Folate Concentrations are Associated with ARA, EPA and DHA Fatty Acids in Red Blood Cells of Brazilian Children and Adolescents"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2184-4977","authenticated-orcid":false,"given":"F\u00e1bio V.","family":"Ued","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"},{"name":"Department of Health Sciences, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana G.","family":"Mathias","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roseli B. D.","family":"Toffano","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamiris T.","family":"Barros","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ol\u00edmpia R. V.","family":"Almada","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta G.","family":"Salom\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina A.","family":"Coelho-Landell","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-3181","authenticated-orcid":false,"given":"Elaine","family":"Hillesheim","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce M.","family":"Camarneiro","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Simon","family":"Camelo-Junior","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1019-3654","authenticated-orcid":false,"given":"Davi C.","family":"Aragon","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-8876","authenticated-orcid":false,"given":"Sofia","family":"Moco","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Institute of Health Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Campus, Innovation Square, 1015 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kussmann","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Institute of Health Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Campus, Innovation Square, 1015 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6147-3491","authenticated-orcid":false,"given":"Jim","family":"Kaput","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Institute of Health Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Campus, Innovation Square, 1015 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7141-7776","authenticated-orcid":false,"given":"Jacqueline P.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"},{"name":"Department of Health Sciences, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, Bandeirantes Avenue, 3900, Ribeir\u00e3o Preto 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/sj.ijo.0803581","article-title":"Metabolic risk-factor clustering estimation in children: To draw a line across pediatric metabolic syndrome","volume":"31","author":"Brambilla","year":"2007","journal-title":"Int. J. Obes."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S2213-8587(15)00076-5","article-title":"Prevalence of behavioural risk factors for cardiovascular disease in adolescents in low-income and middle-income countries: An individual participant data meta-analysis","volume":"3","author":"Caleyachetty","year":"2015","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1542\/peds.108.3.712","article-title":"Relationship of childhood obesity to coronary heart disease risk factors in adulthood: The Bogalusa Heart Study","volume":"108","author":"Freedman","year":"2001","journal-title":"Pediatrics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1590\/S1519-38292005000100012","article-title":"Alterations in the feeding patterns of adolescents with weight-height adequacy and high body fat percentage","volume":"5","author":"Vieira","year":"2005","journal-title":"Rev. Bras. Sa\u00fade Matern. Infant."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1097\/MOL.0b013e32835a7ed4","article-title":"Main findings from the prospective Cardiovascular Risk in Young Finns Study","volume":"24","author":"Juonala","year":"2013","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.arcmed.2015.06.006","article-title":"Global Overview of the Epidemiology of Atherosclerotic Cardiovascular Disease","volume":"46","author":"Barquera","year":"2015","journal-title":"Arch. Med. Res."},{"key":"ref_7","first-page":"503","article-title":"Increased carotid intima-media thickness and associations with cardiovascular risk factors in obese and overweight children and adolescents","volume":"12","author":"Schiel","year":"2007","journal-title":"Eur. J. Med. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1056\/NEJMoa1010112","article-title":"Childhood Adiposity, Adult Adiposity, and Cardiovascular Risk Factors","volume":"365","author":"Juonala","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5935\/abc.20170121","article-title":"Atualiza\u00e7\u00e3o da Diretriz Brasileira de Dislipidemias e Preven\u00e7\u00e3o da Aterosclerose\u20142017","volume":"109","author":"Faludi","year":"2017","journal-title":"Arq. Bras. Cardiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Simopoulos, A.P. (2016). An Increase in the Omega-6\/Omega-3 Fatty Acid Ratio Increases the Risk for Obesity. Nutrients, 8.","DOI":"10.3390\/nu8030128"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1056\/NEJM199806043382302","article-title":"Association between Multiple Cardiovascular Risk Factors and Atherosclerosis in Children and Young Adults","volume":"338","author":"Berenson","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1542\/peds.2007-1472","article-title":"Usefulness of Childhood Non-High Density Lipoprotein Cholesterol Levels Versus Other Lipoprotein Measures in Predicting Adult Subclinical Atherosclerosis: The Bogalusa Heart Study","volume":"121","author":"Frontini","year":"2008","journal-title":"Pediatrics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1631\/jzus.B1400183","article-title":"Elevated homocysteine levels and risk of cardiovascular and all-cause mortality: A meta-analysis of prospective studies","volume":"16","author":"Peng","year":"2015","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1172\/JCI8342","article-title":"Endothelial dysfunction in a murine model of mild hyperhomocyst(e)inemia","volume":"106","author":"Eberhardt","year":"2000","journal-title":"J. Clin. Investig."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1161\/ATVBAHA.108.164830","article-title":"The Homocysteine Paradox","volume":"28","author":"Rodionov","year":"2008","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s10545-010-9151-1","article-title":"The homocysteine controversy","volume":"34","author":"Smulders","year":"2011","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_17","first-page":"CD006612","article-title":"Homocysteine-lowering interventions for preventing cardiovascular events","volume":"8","author":"Lathyris","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1017\/S0007114516004414","article-title":"Folate and vitamin B12 concentrations are associated with plasma DHA and EPA fatty acids in European adolescents: The Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) study","volume":"117","author":"Iglesia","year":"2017","journal-title":"Br. J. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1136\/bmj.38755.366331.2F","article-title":"Risks and benefits of omega 3 fats for mortality, cardiovascular disease, and cancer: Systematic review","volume":"332","author":"Hooper","year":"2006","journal-title":"Br. Med. J."},{"key":"ref_20","first-page":"39","article-title":"Folate, vitamin B12 and vitamin B6 and one carbon metabolism","volume":"6","author":"Selhub","year":"2002","journal-title":"J. Nutr. Health Aging"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1194\/jlr.D011916","article-title":"Specificity and rate of human and mouse liver and plasma phosphatidylcholine synthesis analyzed in vivo","volume":"52","author":"Pynn","year":"2011","journal-title":"J. Lipid Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/1743-7075-9-49","article-title":"Combined dietary folate, vitamin B-12, and vitamin B-6 intake influences plasma docosahexaenoic acid concentration in rats","volume":"9","author":"Watkins","year":"2012","journal-title":"Nutr. Metab."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1055\/s-0037-1614038","article-title":"Folate administration increases n-3 polyunsaturated fatty acids in rat plasma and tissue lipids","volume":"84","author":"Pita","year":"2000","journal-title":"Thromb. Haemost."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3177\/jnsv.51.129","article-title":"Polyunsaturated fatty acid (PUFA) changes in serum and liver of undernourished rats given dietary vitamin B6 supplementation","volume":"51","author":"Bertrandt","year":"2005","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jchromb.2018.05.013","article-title":"Targeted metabolomics analysis reveals the association between maternal folic acid supplementation and fatty acids and amino acids profiles in rat pups","volume":"1090","author":"Liu","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s11745-006-5074-x","article-title":"A significant inverse relationship between concentrations of plasma homocysteine and phospholipid docosahexaenoic acid in healthy male subjects","volume":"41","author":"Li","year":"2006","journal-title":"Lipids"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/sj.ejcn.1602321","article-title":"The relationship between folate and docosahexaenoic acid in men","volume":"60","author":"Umhau","year":"2006","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/S0140-6736(07)61115-5","article-title":"Erythrocyte folate and plasma DHA in the FACIT study","volume":"370","author":"Dullemeijer","year":"2007","journal-title":"Lancet"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/jn\/138.3.551","article-title":"Lowering plasma homocysteine concentrations of older men and women with folate, vitamin B-12, and vitamin B-6 does not affect the proportion of (n-3) long chain polyunsaturated fatty acids in plasma phosphatidylcholine","volume":"138","author":"Crowe","year":"2008","journal-title":"J. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3945\/jn.112.163246","article-title":"Marginal vitamin B-6 deficiency decreases plasma (n-3) and (n-6) PUFA concentrations in healthy men and women","volume":"142","author":"Zhao","year":"2012","journal-title":"J. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1700613","DOI":"10.1002\/mnfr.201700613","article-title":"Clinical and Vitamin Response to a Short-Term Multi-Micronutrient Intervention in Brazilian Children and Teens: From Population Data to Interindividual Responses","volume":"62","author":"Mathias","year":"2018","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_32","unstructured":"Tanner, J. (1962). Growth at Adolescence, Blackwell Scientific Publications. [2nd ed.]."},{"key":"ref_33","unstructured":"Associa\u00e7\u00e3o Brasileira de Empresas de Pesquisa (2011). Crit\u00e9rio de Classifica\u00e7\u00e3o Econ\u00f4mica Brasil, Associa\u00e7\u00e3o Brasileira de Empresas de Pesquisa."},{"key":"ref_34","unstructured":"Jellife, D. (1968). Evaluacion del Estado de Nutricion de la Comunidad, Organizacion Mundial de la Salud."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"660","DOI":"10.2471\/BLT.07.043497","article-title":"Development of a WHO growth reference for school-aged children and adolescents","volume":"85","author":"Onyango","year":"2007","journal-title":"Bull. World Health Organ."},{"key":"ref_36","unstructured":"Andre, D., Pelletier, R., Farringdon, J., Safier, S., Talbott, W., Stone, R., Vyas, N., Trimble, J., Wolf, D., and Vishnubhatla, S. (2019, March 11). The Development of the SenseWear \u00ae Armband, a Revolutionary Energy Assessment Device to Assess Physical Activity and Lifestyle. Available online: https:\/\/pdfs.semanticscholar.org\/e9e1\/15cb6f381a706687982906d45ed28d40bbac.pdf."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1542\/peds.2003-1088-L","article-title":"Physical Activity among Children Attending Preschools","volume":"114","author":"Pate","year":"2004","journal-title":"Pediatrics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1590\/S0034-89102011000400021","article-title":"A revised version of the Healthy Eating Index for the Brazilian population","volume":"45","author":"Previdelli","year":"2011","journal-title":"Rev. Saude Publica"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Toffano, R., Hillesheim, E., Mathias, M., Coelho-Landell, C., Salom\u00e3o, R., Almada, M., Camarneiro, J., Barros, T., Camelo-Junior, J., and Rezzi, S. (2018). Validation of the Brazilian Healthy Eating Index-Revised Using Biomarkers in Children and Adolescents. Nutrients, 10.","DOI":"10.3390\/nu10020154"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.nut.2008.01.008","article-title":"Validation of a food frequency questionnaire for assessing dietary nutrients in Brazilian children 5 to 10 years of age","volume":"24","author":"Fumagalli","year":"2008","journal-title":"Nutrition"},{"key":"ref_41","unstructured":"Monteiro, J., Pfrimer, K., Tremeschin, M., Molina, M., and Chiarello, P. (2007). Consumo Alimentar: Visualizando por\u00e7\u00f5es, Guanabara Koogan. [1st ed.]."},{"key":"ref_42","unstructured":"Institute of Medicine (1998). Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline, National Academies Press (US)."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1016\/S0002-8223(02)90346-9","article-title":"The National Academies Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein and amino acids","volume":"102","author":"Trumbo","year":"2002","journal-title":"J. Am. Diet. Assoc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/j.jada.2010.05.008","article-title":"Validation and Calibration of a Semiquantitative Food Frequency Questionnaire Designed for Adolescents","volume":"110","author":"Araujo","year":"2010","journal-title":"J. Am. Diet. Assoc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1194\/jlr.D500022-JLR200","article-title":"A simplified and efficient method for the analysis of fatty acid methyl esters suitable for large clinical studies","volume":"46","author":"Masood","year":"2005","journal-title":"J. Lipid Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.chroma.2007.08.073","article-title":"Fast analysis by gas\u2013liquid chromatography","volume":"1169","author":"Destaillats","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.4155\/bio-2016-0112","article-title":"High-throughput method for the quantitation of metabolites and co-factors from homocysteine\u2013methionine cycle for nutritional status assessment","volume":"8","author":"Collino","year":"2016","journal-title":"Bioanalysis"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s12263-014-0403-9","article-title":"Methylation potential associated with diet, genotype, protein, and metabolite levels in the Delta Obesity Vitamin Study","volume":"9","author":"Monteiro","year":"2014","journal-title":"Genes Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0378-4347(97)00213-2","article-title":"Measuring S-adenosylmethionine in whole blood, red blood cells and cultured cells using a fast preparation method and high-performance liquid chromatography","volume":"696","author":"Wise","year":"1997","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_50","first-page":"649","article-title":"Blood determinations of S-adenosylmethionine, S-adenosylhomocysteine, and homocysteine: Correlations with diet","volume":"10","author":"Poirier","year":"2001","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.jnutbio.2006.11.010","article-title":"Red blood cell folate vitamer distribution in healthy subjects is determined by the methylenetetrahydrofolate reductase C677T polymorphism and by the total folate status","volume":"18","author":"Smulders","year":"2007","journal-title":"J. Nutr. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.nut.2008.05.018","article-title":"Methylation status in healthy subjects with normal and high serum folate concentration","volume":"24","author":"Hirsch","year":"2008","journal-title":"Nutrition"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.chroma.2015.09.049","article-title":"Simultaneous quantification of 21 water soluble vitamin circulating forms in human plasma by liquid chromatography-mass spectrometry","volume":"1422","author":"Longet","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"ref_54","unstructured":"Cohen, J. (1988). Statistical Power Analysis for the Behavioral Sciences, Academic Press. [2nd ed.]."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1542\/peds.2009-2107C","article-title":"Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents: Summary Report","volume":"128","year":"2011","journal-title":"Pediatrics"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1373\/clinchem.2003.021634","article-title":"Facts and Recommendations about Total Homocysteine Determinations: An Expert Opinion","volume":"50","author":"Refsum","year":"2004","journal-title":"Clin. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1093\/ajcn\/71.4.978","article-title":"Concentrations of riboflavin and related organic acids in children with protein-energy malnutrition","volume":"71","author":"Feillet","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Albersen, M., Bosma, M., Jans, J.J.M., Hofstede, F.C., van Hasselt, P.M., de Sain-van der Velden, M.G.M., Visser, G., and Verhoeven-Duif, N.M. (2015). Vitamin B6 in Plasma and Cerebrospinal Fluid of Children. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0120972"},{"key":"ref_59","unstructured":"Centers for Disease Control and Prevention & National Center for Environmental Health (2014). Second National Report on Biochemical Indicators of Diet and Nutrition in the U.S. Population, 2012, Createspace Independent."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ymgme.2005.08.011","article-title":"Analysis of 5-methyltetrahydrofolate in serum of healthy children","volume":"87","author":"Opladen","year":"2006","journal-title":"Mol. Genet. Metab."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1007\/s11745-014-3935-2","article-title":"Dietary Lipid Intake only Partially Influences Variance in Serum Phospholipid Fatty Acid Composition in Adolescents: Impact of Other Dietary Factors","volume":"49","author":"Vyncke","year":"2014","journal-title":"Lipids"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.plefa.2016.06.004","article-title":"Red blood cell PUFAs reflect the phospholipid PUFA composition of major organs","volume":"112","author":"Fenton","year":"2016","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4828","DOI":"10.1073\/pnas.82.14.4828","article-title":"Phosphatidylcholine produced in rat synaptosomes by N-methylation is enriched in polyunsaturated fatty acids","volume":"82","author":"Tacconi","year":"1985","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1093\/ajcn\/63.6.925","article-title":"Effect of n-3 polyunsaturated fatty acid intake on phospholipid fatty acid composition in plasma and erythrocytes","volume":"63","author":"Prisco","year":"1996","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1271\/bbb.58.459","article-title":"Effect of Vitamin B6 Deficiency on Linoleic Acid Desaturation in the Arachidonic Acid Biosynthesis of Rat Liver Microsomes","volume":"58","author":"She","year":"1994","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0952-3278(98)90163-6","article-title":"Dual influence of aging and vitamin B6 deficiency on delta-6-desaturation of essential fatty acids in rat liver microsomes","volume":"58","author":"Bordoni","year":"1998","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"333S","DOI":"10.1093\/jn\/130.2.333S","article-title":"Effects of vitamin B-6 on (n-3) polyunsaturated fatty acid metabolism","volume":"130","author":"Tsuge","year":"2000","journal-title":"J. Nutr."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1046\/j.1365-2362.2003.01104.x","article-title":"S-adenosylhomocysteine and the ratio of S-adenosylmethionine to S-adenosylhomocysteine are not related to folate, cobalamin and vitamin B6 concentrations","volume":"33","author":"Becker","year":"2003","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1093\/jn\/138.2.311","article-title":"Plasma S-adenosylhomocysteine is a better biomarker of atherosclerosis than homocysteine in apolipoprotein E-deficient mice fed high dietary methionine","volume":"138","author":"Liu","year":"2008","journal-title":"J. Nutr."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1093\/ajcn\/86.6.1581","article-title":"S-Adenosylhomocysteine: A better indicator of vascular disease than homocysteine?","volume":"86","author":"Wagner","year":"2007","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.3945\/ajcn.113.058727","article-title":"Plasma S-adenosylhomocysteine is associated with the risk of cardiovascular events in patients undergoing coronary angiography: A cohort study","volume":"98","author":"Xiao","year":"2013","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1016\/j.neurobiolaging.2006.08.003","article-title":"A metabolic link between S-adenosylhomocysteine and polyunsaturated fatty acid metabolism in Alzheimer\u2019s disease","volume":"28","author":"Selley","year":"2007","journal-title":"Neurobiol. Aging"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1136\/heart.83.2.127","article-title":"Homocysteine, B vitamins, and risk of cardiovascular disease","volume":"83","author":"Robinson","year":"2000","journal-title":"Heart"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1079\/PNS2004390","article-title":"B-vitamins, homocysteine metabolism and CVD","volume":"63","author":"Strain","year":"2004","journal-title":"Proc. Nutr. Soc."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0140-6736(02)07439-1","article-title":"Importance of both folic acid and vitamin B12 in reduction of risk of vascular disease","volume":"359","author":"Quinlivan","year":"2002","journal-title":"Lancet"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1017\/S0029665113003613","article-title":"MTHFR 677TT genotype and disease risk: Is there a modulating role for B-vitamins?","volume":"73","author":"Reilly","year":"2014","journal-title":"Proc. Nutr. Soc."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1093\/jn\/132.2.283","article-title":"The relationship between riboflavin and plasma total homocysteine in the Framingham Offspring cohort is influenced by folate status and the C677T transition in the methylenetetrahydrofolate reductase gene","volume":"132","author":"Jacques","year":"2002","journal-title":"J. Nutr."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1079\/BJN19820070","article-title":"Lipid metabolism in riboflavin-deficient rats. 2. Mitochondrial fatty acid oxidation and the microsomal desaturation pathway","volume":"47","author":"Olpin","year":"1982","journal-title":"Br. J. Nutr."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"185","DOI":"10.5604\/12321966.1228391","article-title":"Influence of protein deficient diet, vitamin B2 supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats","volume":"24","author":"Lewicka","year":"2016","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1007\/s00394-016-1221-1","article-title":"Foods contributing to vitamin B6, folate, and vitamin B12 intakes and biomarkers status in European adolescents: The HELENA study","volume":"56","author":"Iglesia","year":"2017","journal-title":"Eur. J. Nutr."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"674","DOI":"10.3181\/0711-MR-311","article-title":"The Importance of the Omega-6\/Omega-3 Fatty Acid Ratio in Cardiovascular Disease and Other Chronic Diseases","volume":"233","author":"Simopoulos","year":"2008","journal-title":"Exp. Biol. Med."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s00431-009-1035-8","article-title":"Significance of long-chain polyunsaturated fatty acids (PUFAs) for the development and behaviour of children","volume":"169","author":"Schuchardt","year":"2010","journal-title":"Eur. J. Pediatr."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1159\/000471813","article-title":"Folic Acid and Homocysteine in Chronic Kidney Disease and Cardiovascular Disease Progression: Which Comes First?","volume":"7","author":"Cianciolo","year":"2017","journal-title":"Cardiorenal Med."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nm.3762","article-title":"Defective fatty acid oxidation in renal tubular epithelial cells has a key role in kidney fibrosis development","volume":"21","author":"Kang","year":"2015","journal-title":"Nat. Med."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.plipres.2012.08.002","article-title":"Stearoyl-CoA desaturase: Rogue or innocent bystander?","volume":"52","author":"Hodson","year":"2013","journal-title":"Prog. Lipid Res."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"383","DOI":"10.3390\/biology4020383","article-title":"Stearoyl-CoA Desaturase-1: Is It the Link between Sulfur Amino Acids and Lipid Metabolism?","volume":"4","author":"Poloni","year":"2015","journal-title":"Biology"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"E28","DOI":"10.1152\/ajpendo.90897.2008","article-title":"Biochemical and physiological function of stearoyl-CoA desaturase","volume":"297","author":"Paton","year":"2009","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Muthayya, S., Rah, J.H., Sugimoto, J.D., Roos, F.F., Kraemer, K., and Black, R.E. (2013). The Global Hidden Hunger Indices and Maps: An Advocacy Tool for Action. 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