{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:28:42Z","timestamp":1791646122552,"version":"4.3.4"},"reference-count":44,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T00:00:00Z","timestamp":1576454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Researchers have a range of animal models in which to study Nonalcoholic fatty liver disease (NAFLD). Induction of NAFLD by a high-fat diet in the C57BL\/6 strain is the most widely used among mice. In this study, we review works that performed NAFLD induction by a high-fat diet using the C57BL\/6 strain, focusing on experiments on the effects of lipid ingestion. Studies are initially distinguished into researches in which mice received lipids by oral gavage and studies in which lipid was added to the diet, and each of these designs has peculiarities that must be considered. Oral gavage can be stressful for animals and needs trained handlers but allows accurate control of the dose administered. The addition of oils to the diet can prevent stress caused to mice by gavage, but possible changes in the consistency, taste, and smell of the diet should be considered. Regarding the experimental design, some variables, such as animal sex, treatment time, and diet-related variables, appear to have a definite pattern. However, no pattern was found regarding the number of animals per group, age at the beginning of the experiment, time of adaptation, the substance used as a vehicle, and substance used as a control.<\/jats:p>","DOI":"10.3390\/nu11123067","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T02:59:01Z","timestamp":1576551541000},"page":"3067","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":153,"title":["Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in C57bl\/6 Models"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9883-5094","authenticated-orcid":false,"given":"Leonardo","family":"Recena Aydos","sequence":"first","affiliation":[{"name":"Postgraduate Program in Health and Development in the Midwest Region, Medical School, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"},{"name":"Protein Purification Laboratory and its Biological Functions (LPPFB) of the Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luane","family":"Aparecida do Amaral","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health and Development in the Midwest Region, Medical School, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Serafim de Souza","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health and Development in the Midwest Region, Medical School, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"},{"name":"Protein Purification Laboratory and its Biological Functions (LPPFB) of the Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina","family":"Jacobowski","sequence":"additional","affiliation":[{"name":"Protein Purification Laboratory and its Biological Functions (LPPFB) of the Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1528-6035","authenticated-orcid":false,"given":"Elisv\u00e2nia","family":"Freitas dos Santos","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health and Development in the Midwest Region, Medical School, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"},{"name":"Faculty of Pharmaceutical Sciences, Food, and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-6307","authenticated-orcid":false,"given":"Maria L\u00edgia","family":"Rodrigues Macedo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health and Development in the Midwest Region, Medical School, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"},{"name":"Protein Purification Laboratory and its Biological Functions (LPPFB) of the Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"},{"name":"Faculty of Pharmaceutical Sciences, Food, and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/hep.28431","article-title":"Global Epidemiology of Nonalcoholic Fatty Liver Disease\u2014Meta-Analytic Assessment of Prevalence, Incidence, and Outcomes","volume":"64","author":"Younossi","year":"2016","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/liv.13643","article-title":"Global epidemiology of non-alcoholic fatty liver disease\/non-alcoholic steatohepatitis: What we need in the future","volume":"38","author":"Rosso","year":"2018","journal-title":"Liver Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7006","DOI":"10.3748\/wjg.v22.i31.7006","article-title":"Metabolic aspects of adult patients with nonalcoholic fatty liver disease","volume":"22","author":"Abenavoli","year":"2016","journal-title":"World J. 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