{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:38:34Z","timestamp":1761745114927,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T00:00:00Z","timestamp":1577404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011262","name":"FP7 Food, Agriculture and Fisheries, Biotechnology","doi-asserted-by":"publisher","award":["311876"],"award-info":[{"award-number":["311876"]}],"id":[{"id":"10.13039\/100011262","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Docosahexaenoic acid (DHA) has been reported to have a positive impact on many diet-related disease risks, including metabolic syndrome. Although many DHA-enriched foods have been marketed, the impact of different food matrices on the effect of DHA is unknown. As well, the possibility to enhance DHA effectiveness through the co-administration of other bioactives has seldom been considered. We evaluated DHA effects on the serum metabolome administered to volunteers at risk of metabolic syndrome as an ingredient of three different foods. Foods were enriched with DHA alone or in combination with oat beta-glucan or anthocyanins and were administered to volunteers for 4 weeks. Serum samples collected at the beginning and end of the trial were analysed by NMR-based metabolomics. Multivariate and univariate statistical analyses were used to characterize modifications in the serum metabolome and to evaluate bioactive-bioactive and bioactive-food matrix interactions. DHA administration induces metabolome perturbation that is influenced by the food matrix and the co-presence of other bioactives. In particular, when co-administered with oat beta-glucan, DHA induces a strong rearrangement in the lipoprotein profile of the subjects. The observed modifications are consistent with clinical results and indicate that metabolomics represents a possible strategy to choose the most appropriate food matrices for bioactive enrichment.<\/jats:p>","DOI":"10.3390\/nu12010086","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T11:42:47Z","timestamp":1577446967000},"page":"86","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["DHA-Induced Perturbation of Human Serum Metabolome. Role of the Food Matrix and Co-Administration of Oat \u03b2-glucan and Anthocyanins"],"prefix":"10.3390","volume":"12","author":[{"given":"Veronica","family":"Ghini","sequence":"first","affiliation":[{"name":"Center of Magnetic Resonance (CERM), University of Florence, via Luigi Sacconi, 6-50019 Sesto Fiorentino (FI), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6438-059X","authenticated-orcid":false,"given":"Leonardo","family":"Tenori","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, Largo Brambilla, 3-50134 Florence (FI), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5543-2359","authenticated-orcid":false,"given":"Francesco","family":"Capozzi","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences, Alma Mater Studiorum University of Bologna, Piazza G. Goidanich, 60, 47521 Cesena (FC), Italy"},{"name":"Interdepartmental Centre for Industrial Agrofood Research, Alma Mater Studiorum University of Bologna, Via Q. Bucci 336, 47521 Cesena (FC), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2271-8921","authenticated-orcid":false,"given":"Claudio","family":"Luchinat","sequence":"additional","affiliation":[{"name":"Center of Magnetic Resonance (CERM), University of Florence, via Luigi Sacconi, 6-50019 Sesto Fiorentino (FI), Italy"},{"name":"Department of Chemistry, University of Florence, via della Lastruccia, 3-50019 Sesto Fiorentino (FI), Italy"},{"name":"GIOTTO Biotech S.r.l., Via Madonna del Piano, 6-50019 Sesto Fiorentino (FI), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6907-5519","authenticated-orcid":false,"given":"Achim","family":"Bub","sequence":"additional","affiliation":[{"name":"Max Rubner-Institute, Federal Research Centre for Nutrition and Food, Haid-und-NeuStrasse 9, DE-76131 Karlsruhe, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne","family":"Malpuech-Brugere","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, INRA, UNH, Unit\u00e9 de Nutrition Humaine, CRNH Auvergne, F63000 Clermont-Ferrand, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Orfila","sequence":"additional","affiliation":[{"name":"School of Food Science and Nutrition, University of Leeds, Woodhouse Ln, Leeds LS2 9JT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2568-6208","authenticated-orcid":false,"given":"Luigi","family":"Ricciardiello","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, via Massarenti 9, 40138 Bologna (BO), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4579-1662","authenticated-orcid":false,"given":"Alessandra","family":"Bordoni","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences, Alma Mater Studiorum University of Bologna, Piazza G. Goidanich, 60, 47521 Cesena (FC), Italy"},{"name":"Interdepartmental Centre for Industrial Agrofood Research, Alma Mater Studiorum University of Bologna, Via Q. Bucci 336, 47521 Cesena (FC), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"569486","DOI":"10.1155\/2012\/569486","article-title":"Functional foods and nutraceuticals in the primary prevention of cardiovascular diseases","volume":"2012","author":"Alissa","year":"2012","journal-title":"J. Nutr. Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/j.1753-4887.2012.00492.x","article-title":"Worldwide consumption of functional foods: A systematic review","volume":"70","author":"Ozen","year":"2012","journal-title":"Nutr. 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