{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:23:41Z","timestamp":1780068221633,"version":"3.54.0"},"reference-count":67,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T00:00:00Z","timestamp":1578528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Turku University Foundation","award":["080525"],"award-info":[{"award-number":["080525"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 \u00b0C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.<\/jats:p>","DOI":"10.3390\/nu12010182","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T04:06:51Z","timestamp":1578629211000},"page":"182","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8485-2224","authenticated-orcid":false,"given":"Anastasia","family":"Mantziari","sequence":"first","affiliation":[{"name":"Functional Foods Forum, Faculty of Medicine, University of Turku, It\u00e4inen Pitk\u00e4katu 4A, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satu","family":"T\u00f6lkk\u00f6","sequence":"additional","affiliation":[{"name":"Functional Foods Forum, Faculty of Medicine, University of Turku, It\u00e4inen Pitk\u00e4katu 4A, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8348-0633","authenticated-orcid":false,"given":"Artur C.","family":"Ouwehand","sequence":"additional","affiliation":[{"name":"DuPont Nutrition and Biosciences, Sokeritehtaantie 20, 02460 Kantvik, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7278-6511","authenticated-orcid":false,"given":"Eliisa","family":"L\u00f6yttyniemi","sequence":"additional","affiliation":[{"name":"Unit of Biostatistics, Department of Clinical Medicine University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Isolauri","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Turku and Turku University Hospital, Kiinamyllynkatu 4-8, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seppo","family":"Salminen","sequence":"additional","affiliation":[{"name":"Functional Foods Forum, Faculty of Medicine, University of Turku, It\u00e4inen Pitk\u00e4katu 4A, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuli","family":"Rautava","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Turku and Turku University Hospital, Kiinamyllynkatu 4-8, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,9]]},"reference":[{"key":"ref_1","unstructured":"United Nations Inter-Agency Group for Child Mortality Estimation (UN IGME) (2019). 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