{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:00:30Z","timestamp":1787029230329,"version":"build-2736575974"},"reference-count":49,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T00:00:00Z","timestamp":1580342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014189","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry","doi-asserted-by":"publisher","award":["117041-03-3-SB010"],"award-info":[{"award-number":["117041-03-3-SB010"]}],"id":[{"id":"10.13039\/501100014189","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and ICT grant","award":["2019A000022"],"award-info":[{"award-number":["2019A000022"]}]},{"name":"INNOPOLIS Foundation, Ministry of Science and ICT grant","award":["2019-DD-RD-0088"],"award-info":[{"award-number":["2019-DD-RD-0088"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Natural herbal medicines have been developed for the treatment and prevention of women\u2019s menopausal symptoms. In this study, we investigated the anti-menopausal effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) extracts in 3T3-L1 preadipocytes, MC3T3-E1 preosteoblasts, and COV434 granulosa cells in vitro and ovariectomized (OVX) ddY mice in vivo. Combination treatment of CO and RF extract at 7:3 ratio inhibited lipid accumulation via Plin1 and Adipoq downregulation in a cocktail of dexamethasone, 3-isobutyl-1-methylxanthine, and insulin (DMI)-induced differentiated 3T3-L1 cells. In addition, CO + RF treatment significantly enhanced osteoblastic differentiation, with mineralized nodule formation occurring through the upregulation of osteoblast-inducing markers in osteoblastic MC3T3-E1 cells. Increased production of estradiol and mRNA expression of ER\u03b1 (ESR1) were observed in androstenedione-induced COV434 granulosa cells treated with the CO + RF extract. In CO + RF-treated mice, fatty hepatocyte deposition and abdominal visceral fat tissues reduced with OVX-induced uterine atrophy. Furthermore, bone mineral density and bone mineral content were significantly enhanced by CO + RF in mouse models of ovariectomy-induced femoral bone loss. Taken together, our findings suggested that CO + RF promoted estrogenic activity and had anti-obesity and anti-osteoporotic effects in vitro and in vivo. Thus, a combination of CO and RF extracts may be a good therapeutic strategy for managing women\u2019s menopausal syndromes.<\/jats:p>","DOI":"10.3390\/nu12020369","type":"journal-article","created":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T05:55:46Z","timestamp":1580450146000},"page":"369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Anti-Menopausal Effects of Cornus officinalis and Ribes fasciculatum Extract In Vitro and In Vivo"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1928-5531","authenticated-orcid":false,"given":"Eunkuk","family":"Park","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea"},{"name":"Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunguk","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea"},{"name":"Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subin","family":"Yeo","sequence":"additional","affiliation":[{"name":"Nine B Co. Ltd., Daejeon 34121, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonjoong","family":"Yong","sequence":"additional","affiliation":[{"name":"Nine B Co. Ltd., Daejeon 34121, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junga","family":"Yang","sequence":"additional","affiliation":[{"name":"Nine B Co. Ltd., Daejeon 34121, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0625-3530","authenticated-orcid":false,"given":"Seon-Yong","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea"},{"name":"Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea"},{"name":"Nine B Co. Ltd., Daejeon 34121, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,30]]},"reference":[{"key":"ref_1","first-page":"64","article-title":"The menopause and sexual functioning: A review of the population-based studies","volume":"14","author":"Dennerstein","year":"2003","journal-title":"Annu. Rev. Sex. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1464-5491.2006.01858.x","article-title":"Metabolic syndrome\u2014A new world-wide definition. A consensus statement from the international diabetes federation","volume":"23","author":"Alberti","year":"2006","journal-title":"Diabet. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/S0002-9343(99)00063-7","article-title":"Postmenopausal hormone therapy and the risk of colorectal cancer: A review and meta-analysis","volume":"106","author":"Grodstein","year":"1999","journal-title":"Am. J. 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