{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:48:13Z","timestamp":1760237293906,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T00:00:00Z","timestamp":1585008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Food ingestion has been shown to affect thermoregulation during exercise, while the impact of protein degradant consumption remains unclear. We investigated the effects of casein hydrolysate ingestion on thermoregulatory responses during exercise in the heat. In a randomized, placebo-controlled, double-blind, crossover trial, five men and five women consumed either 5 g of casein hydrolysate or placebo. Thirty minutes after ingestion, participants cycled at 60% VO2max until voluntary exhaustion wearing a hot-water (43 \u00b0C) circulation suit. Exercise time to exhaustion, body core temperature, forearm sweat rate, and forearm cutaneous vascular conductance did not differ different between the conditions. However, chest sweat rate and mean skin temperature increased upon casein hydrolysate ingestion compared with placebo during exercise. Increased chest sweat rate upon casein hydrolysate ingestion was associated with elevated sudomotor sensitivity to increasing body core temperature, but not the temperature threshold for initiating sweating. A positive correlation was found between chest sweat rate and plasma total amino acid concentration during exercise. These results suggest that casein hydrolysate ingestion enhances sweating heterogeneously by increasing peripheral sensitivity of the chest\u2019s sweating mechanism and elevating skin temperature during exercise in the heat. However, the physiological link between plasma amino acid concentration and sweat rate remains unclear.<\/jats:p>","DOI":"10.3390\/nu12030867","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T13:04:04Z","timestamp":1585055044000},"page":"867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Casein Hydrolysate Ingestion on Thermoregulatory Responses in Healthy Adults during Exercise in Heated Conditions: A Randomized Crossover Trial"],"prefix":"10.3390","volume":"12","author":[{"given":"Yasuyuki","family":"Sakata","sequence":"first","affiliation":[{"name":"Wellness &amp; Nutrition Science Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa-Pref. 252-8583, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikako","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Laboratory for Exercise and Environmental Physiology, Faculty of Education, Niigata University, Niigata, Niigata-Pref. 950-2181, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Fujiki","sequence":"additional","affiliation":[{"name":"Laboratory for Exercise and Environmental Physiology, Faculty of Education, Niigata University, Niigata, Niigata-Pref. 950-2181, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Wellness &amp; Nutrition Science Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa-Pref. 252-8583, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohiko","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Wellness &amp; Nutrition Science Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa-Pref. 252-8583, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Wellness &amp; Nutrition Science Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa-Pref. 252-8583, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Takeda","sequence":"additional","affiliation":[{"name":"Wellness &amp; Nutrition Science Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa-Pref. 252-8583, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuro","family":"Amano","sequence":"additional","affiliation":[{"name":"Laboratory for Exercise and Environmental Physiology, Faculty of Education, Niigata University, Niigata, Niigata-Pref. 950-2181, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1002\/cphy.c130012","article-title":"Performance in the heat-physiological factors of importance for hyperthermia-induced fatigue","volume":"4","author":"Nybo","year":"2014","journal-title":"Compr. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1002\/cphy.c100082","article-title":"Integrated physiological mechanisms of exercise performance, adaptation, and maladaptation to heat stress","volume":"1","author":"Sawka","year":"2011","journal-title":"Compr. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1152\/jappl.1999.86.3.1032","article-title":"Influence of body temperature on the development of fatigue during prolonged exercise in the heat","volume":"86","author":"Teller","year":"1999","journal-title":"J. Appl. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1113\/jphysiol.1993.sp019482","article-title":"Human circulatory and thermoregulatory adaptations with heat acclimation and exercise in a hot, dry environment","volume":"460","author":"Nielsen","year":"1993","journal-title":"J. Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1249\/MSS.0b013e3181adb9fb","article-title":"Aerobic performance is degraded, despite modest hyperthermia, in hot environments","volume":"42","author":"Ely","year":"2010","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1113\/expphysiol.2011.061002","article-title":"High skin temperature and hypohydration impair aerobic performance","volume":"97","author":"Sawka","year":"2012","journal-title":"Exp. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1152\/jappl.1999.86.2.479","article-title":"Respiratory gas-exchange ratios during graded exercise in fed and fasted trained and untrained men","volume":"86","author":"Bergman","year":"1999","journal-title":"J. Appl. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1139\/apnm-2018-0069","article-title":"Effect of food intake on the ventilatory response to increasing core temperature during exercise","volume":"44","author":"Hayashi","year":"2019","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1139\/apnm-2013-0506","article-title":"Effect of postprandial thermogenesis on the cutaneous vasodilatory response during exercise","volume":"39","author":"Hayashi","year":"2014","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_10","unstructured":"GA, B. (1978). The biochemistry of energy expenditure. Recent Advances in Obesity Research, Newman Publishing."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/s41586-019-1503-x","article-title":"BCAA catabolism in brown fat controls energy homeostasis through SLC25A44","volume":"572","author":"Yoneshiro","year":"2019","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"525","DOI":"10.3945\/ajcn.110.005850","article-title":"Protein choices targeting thermogenesis and metabolism","volume":"93","author":"Acheson","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1136\/gut.9.4.425","article-title":"Absorption and malabsorption of glycine and glycine peptides in man","volume":"9","author":"Craft","year":"1968","journal-title":"Gut"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Matsunaga, Y., Sakata, Y., Yago, T., Nakamura, H., Shimizu, T., and Takeda, Y. (2018). Effects of Glucose with Casein Peptide Supplementation on Post-Exercise Muscle Glycogen Resynthesis in C57BL\/6J Mice. Nutrients, 10.","DOI":"10.3390\/nu10060753"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"377","DOI":"10.7600\/jpfsm.4.377","article-title":"Pre-exercise casein peptide supplementation enhances endurance training-induced mitochondrial enzyme activity in slow twitch muscle, but not fast twitch muscle of high fat diet-fed mice","volume":"4","author":"Matsunaga","year":"2015","journal-title":"J. Phys. Fit. Sports Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1152\/japplphysiol.00357.2004","article-title":"Branched-chain amino acid supplementation and human performance when hypohydrated in the heat","volume":"97","author":"Cheuvront","year":"2004","journal-title":"J. Appl. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1249\/MSS.0000000000000243","article-title":"Failure of oral tyrosine supplementation to improve exercise performance in the heat","volume":"46","author":"Tumilty","year":"2014","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s00421-015-3295-5","article-title":"Acute L-arginine supplementation has no effect on cardiovascular or thermoregulatory responses to rest, exercise, and recovery in the heat","volume":"116","author":"Tyler","year":"2016","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1097\/00000542-200403000-00025","article-title":"Effect of amino acid infusion on central thermoregulatory control in humans","volume":"100","author":"Nakajima","year":"2004","journal-title":"Anesthesiology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1152\/jappl.1971.31.6.828","article-title":"Peripheral modifications to the central drive for sweating","volume":"31","author":"Nadel","year":"1971","journal-title":"J. Appl. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1292\/jvms.16-0450","article-title":"Elephant\u2019s breast milk contains large amounts of glucosamine","volume":"79","author":"Takatsu","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.5432\/ijshs.201223","article-title":"Milk Peptide Intake May Decrease Muscle Damage after Eccentric Exercise","volume":"11","author":"Hirose","year":"2013","journal-title":"Int. J. Sport Health Sci."},{"key":"ref_23","first-page":"377","article-title":"American College of Sports Medicine position stand. Exercise and fluid replacement","volume":"39","author":"Sawka","year":"2007","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1007\/s00421-005-1329-0","article-title":"Effects of physical training on heat loss responses of young women to passive heating in relation to menstrual cycle","volume":"94","author":"Kuwahara","year":"2005","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1113\/EP086752","article-title":"Fitness-related differences in the rate of whole-body total heat loss in exercising young healthy women are heat-load dependent","volume":"103","author":"Lamarche","year":"2018","journal-title":"Exp. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1046\/j.1365-201x.2002.00921.x","article-title":"Sweating response in physically trained men to sustained handgrip exercise in mildly hyperthermic conditions","volume":"174","author":"Yanagimoto","year":"2002","journal-title":"Acta. Physiol. Scand."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1152\/japplphysiol.00646.2010","article-title":"Skin blood flow and local temperature independently modify sweat rate during passive heat stress in humans","volume":"109","author":"Wingo","year":"2010","journal-title":"J. Appl. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1152\/japplphysiol.00370.2018","article-title":"Cutaneous adrenergic nerve blockade attenuates sweating during incremental exercise in habitually trained men","volume":"125","author":"Amano","year":"2018","journal-title":"J. Appl. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"R1332","DOI":"10.1152\/ajpregu.00545.2015","article-title":"Influence of forearm muscle metaboreceptor activation on sweating and cutaneous vascular responses during dynamic exercise","volume":"310","author":"Amano","year":"2016","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_30","first-page":"313","article-title":"Assessment of man\u2019s thermal comfort in practice","volume":"30","author":"Fanger","year":"1973","journal-title":"Br. J. Ind. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1249\/mss.0b013e318050ca3e","article-title":"Temporal Thermometry Fails to Track Body Core Temperature during Heat Stress","volume":"39","author":"Low","year":"2007","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1152\/jappl.1964.19.3.531","article-title":"A new weighting system for mean surface temperature of the hunan body","volume":"19","author":"Ramanathan","year":"1964","journal-title":"J. Appl. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1152\/jappl.1966.21.3.967","article-title":"Partitional calorimetric studies of responses of man to thermal transients","volume":"21","author":"Stolwijk","year":"1966","journal-title":"J. Appl. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1152\/japplphysiol.00250.2009","article-title":"A simple and valid method to determine thermoregulatory sweating threshold and sensitivity","volume":"107","author":"Cheuvront","year":"2009","journal-title":"J. Appl. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s00394-004-0448-4","article-title":"Gastric emptying, gastric secretion and enterogastrone response after administration of milk proteins or their peptide hydrolysates in humans","volume":"43","author":"Calbet","year":"2004","journal-title":"Eur. J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1038\/ijo.2016.124","article-title":"Brown adipose tissue is involved in diet-induced thermogenesis and whole-body fat utilization in healthy humans","volume":"40","author":"Hibi","year":"2016","journal-title":"Int. J. Obes."},{"key":"ref_37","unstructured":"Newsholme, E.A., and Leech, T.R. (2010). Functional Biochemistry in Health and Disease, Wiley-Blackwell."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1152\/japplphysiol.01124.2005","article-title":"Neural control and mechanisms of eccrine sweating during heat stress and exercise","volume":"100","author":"Shibasaki","year":"2006","journal-title":"J. Appl. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1126\/science.147.3664.1465","article-title":"Sweating: Direct influence of skin temperature","volume":"147","author":"Bullard","year":"1965","journal-title":"Science"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s00380-010-0096-y","article-title":"Milk casein-derived tripeptides, VPP and IPP induced NO production in cultured endothelial cells and endothelium-dependent relaxation of isolated aortic rings","volume":"26","author":"Hirota","year":"2011","journal-title":"Heart Vessels"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.foodchem.2014.09.098","article-title":"Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro","volume":"172","author":"Yamada","year":"2015","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/sms.12349","article-title":"Human behavioral thermoregulation during exercise in the heat","volume":"25","author":"Flouris","year":"2015","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.physbeh.2011.02.002","article-title":"The independent roles of temperature and thermal perception in the control of human thermoregulatory behavior","volume":"103","author":"Schlader","year":"2011","journal-title":"Physiol. Behav."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.jtherbio.2011.07.002","article-title":"Evidence for thermoregulatory behavior during self-paced exercise in the heat","volume":"36","author":"Schlader","year":"2011","journal-title":"J. Therm. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"R990","DOI":"10.1152\/ajpregu.00005.2015","article-title":"Modulation of muscle metaboreceptor activation upon sweating and cutaneous vascular responses to rising core temperature in humans","volume":"308","author":"Amano","year":"2015","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/3\/867\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:11:03Z","timestamp":1760173863000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/3\/867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,24]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["nu12030867"],"URL":"https:\/\/doi.org\/10.3390\/nu12030867","relation":{},"ISSN":["2072-6643"],"issn-type":[{"type":"electronic","value":"2072-6643"}],"subject":[],"published":{"date-parts":[[2020,3,24]]}}}