{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:09:52Z","timestamp":1788613792879,"version":"build-2803163510"},"reference-count":56,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,18]],"date-time":"2020-04-18T00:00:00Z","timestamp":1587168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The study investigated the protective effect of walnut oligopeptides (WOPs) against ethanol-induced gastric injury using Sprague-Dawley (SD) rats. Rats were randomly divided into seven groups based on body weight (10\/group), normal group, ethanol group, whey protein group (220 mg\/kg body weight), omeprazole group (20 mg\/kg body weight), and three WOPs groups (220, 440, 880 mg\/kg body weight). After 30 days of treatment with WOPs, rats were given 5 mL\/kg absolute ethanol by gavage to induce gastric mucosal injury. Gastric ulcer index (GUI) were determined and the following measured; gastric content pH, gastric mucin, endogenous pepsinogens (PG), prostaglandin E2 (PGE2), inflammatory cytokines, oxidative stress indicators, and the expression of apoptosis-related proteins were measured to evaluate the gastroprotective effect of WOPs. The results showed that the administration with WOPs markedly mitigated the hemorrhagic gastric lesions caused by ethanol in rats, and decreased the GUI, the gastric content pH, PG1, PG2, and NO levels, enhanced mucin and PGE2. Also, WOPs repressed gastric inflammation through the reduction of TNF-\u03b1, IL-6, IL-1\u03b2 and increase IL-10 levels, and revealed antioxidant properties with the enhancement of superoxide dismutase, glutathione, and catalase activity, while reduction of malondialdehyde. Moreover, WOPs treatment significantly down-regulated Bax, caspase-3 and nuclear factor-\u03baB p65 (NF-\u03baB p65) expression, while up-regulating the expression of Bcl-2 and inhibitor kappa B\u03b1 (I\u03baB\u03b1) protein. These results indicated that WOPs have protective effects against ethanol-induced gastric mucosal injury in rats through anti-inflammatory, anti-oxidation, and anti-apoptosis mechanisms.<\/jats:p>","DOI":"10.3390\/nu12041138","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T04:49:38Z","timestamp":1587444578000},"page":"1138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats"],"prefix":"10.3390","volume":"12","author":[{"given":"Rui","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Tao","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Ran","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Wei","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Xue","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3914-5840","authenticated-orcid":false,"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.path.2017.07.003","article-title":"Patterns of Gastric Injury: Beyond Helicobacter Pylori","volume":"10","author":"Choi","year":"2017","journal-title":"Surg. Pathol. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sidahmed, H.M., Hashim, N.M., Abdulla, M.A., Ali, H.M., Mohan, S., Abdelwahab, S.I., Taha, M.M., Fai, L.M., and Vadivelu, J. (2015). Antisecretory, gastroprotective, antioxidant and anti-Helicobcter pylori activity of zerumbone from Zingiber zerumbet (L.) Smith. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0121060"},{"key":"ref_3","unstructured":"(2018). Global Status Report on Alcohol and Health 2018, World Health Organization. Available online: https:\/\/www.who.int\/substance_abuse\/publications\/global_alcohol_report\/en\/."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Al-Wajeeh, N.S., Halabi, M.F., Hajrezaie, M., Dhiyaaldeen, S.M., Abdulaziz Bardi, D., Salama, S.M., Rouhollahi, E., Karimian, H., Abdolmalaki, R., and Azizan, A.H. (2016). The Gastroprotective Effect of Vitex pubescens Leaf Extract against Ethanol-Provoked Gastric Mucosal Damage in Sprague-Dawley Rats. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0157431"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"974185","DOI":"10.1155\/2013\/974185","article-title":"Acute toxicity and gastroprotective role of M. pruriens in ethanol-induced gastric mucosal injuries in rats","volume":"2013","author":"Golbabapour","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1152\/physrev.00040.2012","article-title":"Oxidative stress: An essential factor in the pathogenesis of gastrointestinal mucosal diseases","volume":"94","author":"Bhattacharyya","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Meyer, A.R., and Goldenring, J.R. (2018). Injury, repair, inflammation and metaplasia in the stomach. J. Physiol.","DOI":"10.1113\/JP275512"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ijbiomac.2018.01.008","article-title":"Momordica charantia polysaccharides ameliorate oxidative stress, inflammation, and apoptosis in ethanol-induced gastritis in mucosa through NF-kB signaling pathway inhibition","volume":"111","author":"Raish","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.2174\/1381612033454892","article-title":"Food-derived bioactive peptides\u2014Opportunities for designing future foods","volume":"9","author":"Korhonen","year":"2003","journal-title":"Curr. Pharm. Des."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1039\/C7FO00957G","article-title":"Ginseng (Panax ginseng Meyer) oligopeptides regulate innate and adaptive immune responses in mice via increased macrophage phagocytosis capacity, NK cell activity and Th cells secretion","volume":"8","author":"He","year":"2017","journal-title":"Food Funct."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jnutbio.2016.09.019","article-title":"Suppression of TNF-alpha and free radicals reduces systematic inflammatory and metabolic disorders: Radioprotective effects of ginseng oligopeptides on intestinal barrier function and antioxidant defense","volume":"40","author":"He","year":"2017","journal-title":"J. Nutr. Biochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4266","DOI":"10.1039\/C7FO01658A","article-title":"Anti-fatigue effects of small-molecule oligopeptides isolated from Panax quinquefolium L. in mice","volume":"9","author":"Li","year":"2018","journal-title":"Food Funct."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Xu, M., Sun, B., Li, D., Mao, R., Li, H., Li, Y., and Wang, J. (2017). Beneficial Effects of Small Molecule Oligopeptides Isolated from Panax ginseng Meyer on Pancreatic Beta-Cell Dysfunction and Death in Diabetic Rats. Nutrients, 9.","DOI":"10.3390\/nu9101061"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1089\/jmf.2008.0277","article-title":"Anti-ulcerogenic effect of a whey protein isolate and collagen hydrolysates against ethanol ulcerative lesions on oral administration to rats","volume":"13","author":"Castro","year":"2010","journal-title":"J. Med. Food"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kan, J., Hood, M., Burns, C., Scholten, J., Chuang, J., Tian, F., Pan, X., Du, J., and Gui, M. (2017). A Novel Combination of Wheat Peptides and Fucoidan Attenuates Ethanol-Induced Gastric Mucosal Damage through Anti-Oxidant, Anti-Inflammatory, and Pro-Survival Mechanisms. Nutrients, 9.","DOI":"10.3390\/nu9090978"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"H1807","DOI":"10.1111\/1750-3841.13332","article-title":"Protective Effect of Cod (Gadus macrocephalus) Skin Collagen Peptides on Acetic Acid-Induced Gastric Ulcer in Rats","volume":"81","author":"Niu","year":"2016","journal-title":"J. Food Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.peptides.2012.09.017","article-title":"Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates","volume":"38","author":"Chen","year":"2012","journal-title":"Peptides"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ma, S., Huang, D., Zhai, M., Yang, L., Peng, S., Chen, C., Feng, X., Weng, Q., Zhang, B., and Xu, M. (2015). Isolation of a novel bio-peptide from walnut residual protein inducing apoptosis and autophagy on cancer cells. BMC Complement. Altern. Med., 15.","DOI":"10.1186\/s12906-015-0940-9"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1016\/j.jnutbio.2012.06.009","article-title":"Walnut diet reduces accumulation of polyubiquitinated proteins and inflammation in the brain of aged rats","volume":"24","author":"Poulose","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11767","DOI":"10.1021\/jf504672m","article-title":"Characterization of low molecular weight allergens from English walnut (Juglans regia)","volume":"62","author":"Downs","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.5625\/lar.2013.29.4.190","article-title":"Walnut extract exhibits anti-fatigue action via improvement of exercise tolerance in mice","volume":"29","author":"Kim","year":"2013","journal-title":"Lab. Anim. Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Liu, R., Wu, L., Du, Q., Ren, J.W., Chen, Q.H., Li, D., Mao, R.X., Liu, X.R., and Li, Y. (2018). Small Molecule Oligopeptides Isolated from Walnut (Juglans regia L.) and Their Anti-Fatigue Effects in Mice. Molecules, 24.","DOI":"10.3390\/molecules24010045"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhu, N., Liu, R., He, L.X., Mao, R.X., Liu, X.R., Zhang, T., Hao, Y.T., Fan, R., Xu, M.H., and Li, Y. (2019). Radioprotective Effect of Walnut Oligopeptides Against Gamma Radiation-Induced Splenocyte Apoptosis and Intestinal Injury in Mice. Molecules, 24.","DOI":"10.3390\/molecules24081582"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.ijbiomac.2017.08.175","article-title":"Protective effects of polysaccharide from Dendrobium nobile against ethanol-induced gastric damage in rats","volume":"107","author":"Zhang","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.alcohol.2015.08.004","article-title":"Therapeutic potential and mechanism of thymol action against ethanol-induced gastric mucosal injury in rat model","volume":"49","author":"Chauhan","year":"2015","journal-title":"Alcohol (Fayettev N.Y.)"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.phymed.2014.10.002","article-title":"Protective role of ellagitannins from Eucalyptus citriodora against ethanol-induced gastric ulcer in rats: Impact on oxidative stress, inflammation and calcitonin-gene related peptide","volume":"22","year":"2015","journal-title":"Phytomedicine"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Jeon, W.Y., Shin, I.S., Shin, H.K., and Lee, M.Y. (2014). Gastroprotective effect of the traditional herbal medicine, Sipjeondaebo-tang water extract, against ethanol-induced gastric mucosal injury. BMC Complement. Altern. Med., 14.","DOI":"10.1186\/1472-6882-14-373"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"361","DOI":"10.3892\/mmr.2016.5269","article-title":"Syzygium aromaticum water extract attenuates ethanolinduced gastric injury through antioxidant effects in rats","volume":"14","author":"Jin","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S0016-5085(79)80133-X","article-title":"Topical aspirin plus HCl gastric lesions in the rat. Cytoprotective effect of prostaglandin, cimetidine, and probanthine","volume":"76","author":"Guth","year":"1979","journal-title":"Gastroenterology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cbi.2015.01.015","article-title":"Gastroprotective effect of crocin in ethanol-induced gastric injury in rats","volume":"229","author":"Rizk","year":"2015","journal-title":"Chem. Biol. Interact."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, R., Chen, Q.H., Ren, J.W., Sun, B., Cai, X.X., Li, D., Mao, R.X., Wu, X., and Li, Y. (2018). Ginseng (Panax ginseng Meyer) Oligopeptides Protect Against Binge Drinking-Induced Liver Damage through Inhibiting Oxidative Stress and Inflammation in Rats. Nutrients, 10.","DOI":"10.3390\/nu10111665"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"13916","DOI":"10.1038\/s41598-018-32188-6","article-title":"Ginseng oligopeptides protect against irradiation-induced immune dysfunction and intestinal injury","volume":"8","author":"He","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.drudis.2014.10.003","article-title":"Peptide therapeutics: Current status and future directions","volume":"20","author":"Fosgerau","year":"2015","journal-title":"Drug Discov. Today"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1097\/MED.0b013e3283202555","article-title":"Peptide processing and biology in human disease","volume":"16","author":"Kovac","year":"2009","journal-title":"Curr. Opin. Endocrinol. Diabetes Obes."},{"key":"ref_35","first-page":"93","article-title":"The gastroprotective effects of hydroalcoholic extract of Monolluma quadrangula against ethanol-induced gastric mucosal injuries in Sprague Dawley rats","volume":"10","author":"Ibrahim","year":"2016","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_36","first-page":"879","article-title":"Expression of nerve growth factor in rat stomach. Implications for interactions between endothelial, neural and epithelial cells","volume":"67","author":"Tarnawski","year":"2016","journal-title":"J. Physiol. Pharmacol. Off. J. Pol. Physiol. Soc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1097\/MOG.0000000000000316","article-title":"Gastrointestinal defense mechanisms","volume":"32","author":"Said","year":"2016","journal-title":"Curr. Opin. Gastroenterol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1159\/000478255","article-title":"The Effects of Switching to Vonoprazan, a Novel Potassium-Competitive Acid Blocker, on Gastric Acidity and Reflux Patterns in Patients with Erosive Esophagitis Refractory to Proton Pump Inhibitors","volume":"96","author":"Yamashita","year":"2017","journal-title":"Digestion"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1053\/j.gastro.2008.05.030","article-title":"Gastric mucosal defense and cytoprotection: Bench to bedside","volume":"135","author":"Laine","year":"2008","journal-title":"Gastroenterology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1158\/1078-0432.CCR-06-1404","article-title":"Alpha1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer","volume":"13","author":"Hsu","year":"2007","journal-title":"Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1136\/gutjnl-2011-300420","article-title":"Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage","volume":"61","author":"Demitrack","year":"2012","journal-title":"Gut"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1034\/j.1600-079x.2002.1o811.x","article-title":"Role of prostaglandins, nitric oxide, sensory nerves and gastrin in acceleration of ulcer healing by melatonin and its precursor, L-tryptophan","volume":"32","author":"Brzozowska","year":"2002","journal-title":"J. Pineal Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1177\/0192623316678696","article-title":"Evaluation of Pepsinogen I as a Biomarker of Drug-induced Gastric Mucosal Injury in Cynomolgus Monkeys","volume":"45","author":"Ennulat","year":"2017","journal-title":"Toxicol. Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"458","DOI":"10.4062\/biomolther.2015.052","article-title":"Gastroprotective Activities of Sennoside A and Sennoside B via the Up-Regulation of Prostaglandin E2 and the Inhibition of H(+)\/K(+)-ATPase","volume":"23","author":"Hwang","year":"2015","journal-title":"Biomol. Ther. (Seoul)"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1053\/gast.2002.34178","article-title":"Gastric nitric oxide synthase expression during endotoxemia: Implications in mucosal defense in rats","volume":"123","author":"Helmer","year":"2002","journal-title":"Gastroenterology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s10620-016-4377-x","article-title":"Nitric Oxide and Hydrogen Sulfide Interact When Modulating Gastric Physiological Functions in Rodents","volume":"62","author":"Lucetti","year":"2017","journal-title":"Dig. Dis. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1007\/s10620-017-4681-0","article-title":"Gaseous Mediators in Gastrointestinal Mucosal Defense and Injury","volume":"62","author":"Wallace","year":"2017","journal-title":"Dig. Dis. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Paulrayer, A., Adithan, A., Lee, J.H., Moon, K.H., Kim, D.G., Im, S.Y., Kang, C.W., Kim, N.S., and Kim, J.H. (2017). Aronia melanocarpa (Black Chokeberry) Reduces Ethanol-Induced Gastric Damage via Regulation of HSP-70, NF-kappaB, and MCP-1 Signaling. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061195"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.freeradbiomed.2016.12.048","article-title":"Redox signaling in the gastrointestinal tract","volume":"104","author":"Perez","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1177\/0148607108327046","article-title":"Effect of sesame oil on acidified ethanol-induced gastric mucosal injury in rats","volume":"33","author":"Hsu","year":"2009","journal-title":"JPEN J. Parenter. Enter. Nutr."},{"key":"ref_51","first-page":"1","article-title":"Protective effect of chamomile (Matricaria recutita L.) decoction extract against alcohol-induced injury in rat gastric mucosa","volume":"24","author":"Jabri","year":"2017","journal-title":"Pathophysiol. Off. J. Int. Soc. Pathophysiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4932","DOI":"10.1021\/acs.jafc.7b01135","article-title":"Protective Effect of Camellia Oil (Camellia oleifera Abel.) against Ethanol-Induced Acute Oxidative Injury of the Gastric Mucosa in Mice","volume":"65","author":"Tu","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jep.2015.03.054","article-title":"Possible mechanisms of action of Caesalpinia pyramidalis against ethanol-induced gastric damage","volume":"168","author":"Diniz","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1053\/j.gastro.2009.11.054","article-title":"Conditional deletion of IkappaB-kinase-beta accelerates helicobacter-dependent gastric apoptosis, proliferation, and preneoplasia","volume":"138","author":"Shibata","year":"2010","journal-title":"Gastroenterology"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tang, X., Wei, R., Deng, A., and Lei, T. (2017). Protective Effects of Ethanolic Extracts from Artichoke, an Edible Herbal Medicine, against Acute Alcohol-Induced Liver Injury in Mice. Nutrients, 9.","DOI":"10.3390\/nu9091000"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2898","DOI":"10.1039\/C5FO01580D","article-title":"Dietary nucleotides protect against alcoholic liver injury by attenuating inflammation and regulating gut microbiota in rats","volume":"7","author":"Cai","year":"2016","journal-title":"Food Funct."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/4\/1138\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:31:31Z","timestamp":1760362291000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/4\/1138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,18]]},"references-count":56,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["nu12041138"],"URL":"https:\/\/doi.org\/10.3390\/nu12041138","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,18]]}}}