{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:03:24Z","timestamp":1790168604100,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T00:00:00Z","timestamp":1587600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI13\/01562"],"award-info":[{"award-number":["PI13\/01562"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006280","name":"Ministerio de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["RTI2018-095166-B-I00"],"award-info":[{"award-number":["RTI2018-095166-B-I00"]}],"id":[{"id":"10.13039\/501100006280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Very preterm infants (VPI, born at or before 32 weeks of gestation) are at risk of adverse health outcomes, from which they might be partially protected with appropriate postnatal nutrition and growth. Metabolic processes or biochemical markers associated to extrauterine growth restriction (EUGR) have not been identified. We applied untargeted metabolomics to plasma samples of VPI with adequate weight for gestational age at birth and with different growth trajectories (29 well-grown, 22 EUGR) at the time of hospital discharge. A multivariate analysis showed significantly higher levels of amino-acids in well-grown patients. Other metabolites were also identified as statistically significant in the comparison between groups. Relevant differences (with corrections for multiple comparison) were found in levels of glycerophospholipids, sphingolipids and other lipids. Levels of many of the biochemical species decreased progressively as the level of growth restriction increased in severity. In conclusion, an untargeted metabolomic approach uncovered previously unknown differences in the levels of a range of plasma metabolites between well grown and EUGR infants at the time of discharge. Our findings open speculation about pathways involved in growth failure in preterm infants and the long-term relevance of this metabolic differences, as well as helping in the definition of potential biomarkers.<\/jats:p>","DOI":"10.3390\/nu12041188","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T10:46:22Z","timestamp":1587638782000},"page":"1188","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Plasma Metabolome Alterations Associated with Extrauterine Growth Restriction"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9833-7618","authenticated-orcid":false,"given":"Danuta","family":"Dudzik","sequence":"first","affiliation":[{"name":"Centro deMetabol\u00f3mica y Bioan\u00e1lisis, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, Boadilla del Monte, 28660 Madrid, Spain"},{"name":"Department of Biopharmaceutics and Pharmacodynamics, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Iglesias Platas","sequence":"additional","affiliation":[{"name":"Neonatal Unit, BCNatal, Hospital Sant Joan de D\u00e9u i Cl\u00ednic, Barcelona University, 08950 Barcelona, Spain"},{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4415-8099","authenticated-orcid":false,"given":"Montserrat","family":"Izquierdo Renau","sequence":"additional","affiliation":[{"name":"Neonatal Unit, BCNatal, Hospital Sant Joan de D\u00e9u i Cl\u00ednic, Barcelona University, 08950 Barcelona, Spain"},{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Balcells Esponera","sequence":"additional","affiliation":[{"name":"Neonatal Unit, BCNatal, Hospital Sant Joan de D\u00e9u i Cl\u00ednic, Barcelona University, 08950 Barcelona, Spain"},{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"del Rey Hurtado de Mendoza","sequence":"additional","affiliation":[{"name":"Neonatal Unit, BCNatal, Hospital Sant Joan de D\u00e9u i Cl\u00ednic, Barcelona University, 08950 Barcelona, Spain"},{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0950-4363","authenticated-orcid":false,"given":"Carles","family":"Lerin","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Barcelona, Spain"},{"name":"Endocrinology Department, Hospital Sant Joan de D\u00e9u, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3027-9105","authenticated-orcid":false,"given":"Marta","family":"Ram\u00f3n-Krauel","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Barcelona, Spain"},{"name":"Endocrinology Department, Hospital Sant Joan de D\u00e9u, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4722-491X","authenticated-orcid":false,"given":"Coral","family":"Barbas","sequence":"additional","affiliation":[{"name":"Centro deMetabol\u00f3mica y Bioan\u00e1lisis, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci\u00f3n Montepr\u00edncipe, Boadilla del Monte, 28660 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Izquierdo Renau, M., Aldecoa-bilbao, V., Balcells Esponera, C., del Rey Hurtado de Mendoza, B., Iriondo Sanz, M., and Iglesias Platas, I. (2019). Applying Methods for Postnatal Growth Assessment in the Clinical Setting: Evaluation in a Longitudinal Cohort of Very Preterm Infants. Nutrients, 11.","DOI":"10.3390\/nu11112772"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1203\/PDR.0b013e318217f4f1","article-title":"Early nutrition mediates the influence of severity of illness on extremely LBW infants","volume":"69","author":"Ehrenkranz","year":"2011","journal-title":"Pediatr. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1542\/peds.2005-1368","article-title":"Growth in the Neonatal Intensive Care Unit Influences Neurodevelopmental and Growth Outcomes of Extremely Low Birth Weight Infants","volume":"117","author":"Ehrenkranz","year":"2006","journal-title":"Pediatrics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1111\/apa.12319","article-title":"Poor postnatal head growth in very preterm infants is associated with impaired neurodevelopment outcome","volume":"102","author":"Neubauer","year":"2013","journal-title":"Acta Paediatr. Int. J. Paediatr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e20172169","DOI":"10.1542\/peds.2017-2169","article-title":"Nutrient intake in the first two weeks of life and brain growth in preterm neonates","volume":"141","author":"Schneider","year":"2018","journal-title":"Pediatrics"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1136\/archdischild-2012-302868","article-title":"Increased protein intake decreases postnatal growth faltering in ELBW babies","volume":"98","author":"Cormack","year":"2013","journal-title":"Arch. Dis. Child. Fetal Neonatal Ed."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"F235","DOI":"10.1136\/archdischild-2018-315082","article-title":"Early postnatal growth failure in preterm infants is not inevitable","volume":"104","author":"Andrews","year":"2019","journal-title":"Arch Dis Child Fetal Neonatal Ed"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1177\/0884533616649582","article-title":"Changes in Parenteral Nutrition during the First Week of Life Influence Early but Not Late Postnatal Growth in Very Low-Birth-Weight Infants","volume":"31","author":"Izquierdo","year":"2016","journal-title":"Nutr. Clin. Pract."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1055\/s-0035-1554794","article-title":"Variation in Enteral Feeding Practices and Growth Outcomes among Very Premature Infants: A Report from the New York State Perinatal Quality Collaborative","volume":"33","author":"Stevens","year":"2015","journal-title":"Am. J. Perinatol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pereira-da-silva, L., Virella, D., and Fusch, C. (2019). Nutritional Assessment in Preterm Infants: A Practical Approach in the NICU. Nutrients, 11.","DOI":"10.3390\/nu11091999"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1152\/physrev.00035.2018","article-title":"Metabolomics for investigating physiological and pathophysiological processes","volume":"99","author":"Wishart","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1002\/hep4.1417","article-title":"Development of a Plasma Screening Panel for Pediatric Nonalcoholic Fatty Liver Disease Using Metabolomics","volume":"3","author":"Khusial","year":"2019","journal-title":"Hepatol. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.jpba.2017.02.056","article-title":"GC\u2013MS based Gestational Diabetes Mellitus longitudinal study: Identification of 2-and 3-hydroxybutyrate as potential prognostic biomarkers","volume":"144","author":"Dudzik","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1021\/pr501087x","article-title":"LC - MS-based metabolomics identification of novel biomarkers of chorioamnionitis and its associated perinatal neurological damage","volume":"14","author":"Dudzik","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/ijo.2016.92","article-title":"Insulin resistance in prepubertal obese children correlates with sex-dependent early onset metabolomic alterations","volume":"40","author":"Mastrangelo","year":"2016","journal-title":"Int. J. Obes."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1159\/000365037","article-title":"Rapid growth and childhood obesity are strongly associated with LysoPC(14:0)","volume":"64","author":"Rzehak","year":"2014","journal-title":"Ann. Nutr. Metab."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/ijo.2017.201","article-title":"Untargeted metabolomics identifies a plasma sphingolipid-related signature associated with lifestyle intervention in prepubertal children with obesity","volume":"42","author":"Samino","year":"2018","journal-title":"Int. J. Obes."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/j.clnu.2014.01.002","article-title":"Adaptive regulation of amino acid metabolism on early parenteral lipid and high-dose amino acid administration in VLBW infants - A randomized, controlled trial","volume":"33","author":"Vlaardingerbroek","year":"2014","journal-title":"Clin. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s40168-017-0295-1","article-title":"Longitudinal development of the gut microbiome and metabolome in preterm neonates with late onset sepsis and healthy controls","volume":"5","author":"Stewart","year":"2017","journal-title":"Microbiome"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s40168-016-0216-8","article-title":"Temporal bacterial and metabolic development of the preterm gut reveals specific signatures in health and disease","volume":"4","author":"Stewart","year":"2016","journal-title":"Microbiome"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/pr.2015.235","article-title":"Metabolomic and proteomic analysis of serum from preterm infants with necrotising entercolitis and late-onset sepsis","volume":"79","author":"Stewart","year":"2016","journal-title":"Pediatr. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2310","DOI":"10.1080\/14767058.2018.1432040","article-title":"Is the body composition development in premature infants associated with a distinctive nuclear magnetic resonance metabolomic profiling of urine?","volume":"32","author":"Morniroli","year":"2019","journal-title":"J. Matern. Neonatal Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8167","DOI":"10.1038\/s41598-019-44547-y","article-title":"Disrupted Maturation of the Microbiota and Metabolome among Extremely Preterm Infants with Postnatal Growth Failure","volume":"9","author":"Younge","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_24","unstructured":"Programa de Salut Maternoinfantil, Direcci\u00f3 General de Salut P\u00fablica, D. de S (2008). Corbes de refer\u00e8ncia de pes, per\u00edmetre cranial i longitud en n\u00e9ixer de nounats d \u2019 embarassos \u00fanics, de bessons i de trig\u00e8mins a Catalunya, Prous Science, S. A."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1651-2227.2006.tb02378.x","article-title":"WHO Child Growth Standards based on length\/height, weight and age","volume":"95","author":"Martorell","year":"2006","journal-title":"Acta Paediatr."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Metz, T. (2011). Gas Chromatography-Mass Spectrometry (GC-MS)-Based Metabolomics. Metabolic Profiling. Methods in Molecular Biology, Springer.","DOI":"10.1007\/978-1-61737-985-7"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4849","DOI":"10.1007\/s00216-013-6882-5","article-title":"Method development and validation for rat serum fingerprinting with CE-MS: Application to ventilator-induced-lung-injury study","volume":"405","author":"Naz","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8245","DOI":"10.1021\/ac501853d","article-title":"phase extrac-tion, multiplatform analytical approach Breast milk metabolome characterization in a single phase extrac- tion, multiplatform analytical approach","volume":"86","author":"Posma","year":"2014","journal-title":"Anal Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jpba.2017.07.044","article-title":"Quality assurance procedures for mass spectrometry untargeted metabolomics. a review","volume":"147","author":"Dudzik","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7810","DOI":"10.1039\/C5AN01638J","article-title":"Intra-batch effect correction in liquid chromatography-mass spectrometry using quality control samples and support vector regression (QC-SVRC)","volume":"140","author":"Kuligowski","year":"2015","journal-title":"Analyst"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"W486","DOI":"10.1093\/nar\/gky310","article-title":"MetaboAnalyst 4.0: Towards more transparent and integrative metabolomics analysis","volume":"46","author":"Chong","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1021\/acs.jproteome.8b00720","article-title":"CEU Mass Mediator 3.0: A Metabolite Annotation Tool","volume":"18","author":"Godzien","year":"2019","journal-title":"J. Proteome Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1093\/bioinformatics\/btr661","article-title":"Metscape 2 bioinformatics tool for the analysis and visualization of metabolomics and gene expression data","volume":"28","author":"Karnovsky","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.cmet.2009.02.002","article-title":"A BCAA Related Metabolic Signature that differentiates obese and lean humans contributes to insulin resistance","volume":"9","author":"Newgard","year":"2009","journal-title":"Cell Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.earlhumdev.2015.08.011","article-title":"The potential risks and benefits of insulin treatment in hyperglycaemic preterm neonates","volume":"91","author":"Morgan","year":"2015","journal-title":"Early Hum. Dev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1016\/j.jpeds.2013.12.046","article-title":"Relationship between insulin-like growth factor I levels, early insulin treatment, and clinical outcomes of very low birth weight infants","volume":"164","author":"Beardsall","year":"2014","journal-title":"J. Pediatr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1055\/s-0036-1586109","article-title":"Role of Insulinlike Growth Factor 1 in Fetal Development and in the Early Postnatal Life of Premature Infants","volume":"33","author":"Ley","year":"2016","journal-title":"Am. J. Perinatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1017\/S0007114518001307","article-title":"Reduced plasma concentration of branched-chain amino acids in sarcopenic older subjects: A cross-sectional study","volume":"120","author":"Ottestad","year":"2018","journal-title":"Br. J. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1007\/s12603-018-1014-8","article-title":"Plasma Amino Acid Concentrations Are Associated with Muscle Function in Older Japanese Women","volume":"22","author":"Yamada","year":"2018","journal-title":"J. Nutr. Heal. Aging"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"E640","DOI":"10.1542\/peds.2011-3379","article-title":"Preterm Birth and Body Composition at Term Equivalent Age: A Systematic Review and Meta-analysis","volume":"130","author":"Johnson","year":"2012","journal-title":"Pediatrics"},{"key":"ref_41","first-page":"1","article-title":"Fat trajectory after birth in very preterm infants mimics healthy term infants","volume":"14","author":"Donovan","year":"2019","journal-title":"Pediatr. Obes."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.clnesp.2017.01.003","article-title":"Enhanced nutrient supply to very low birth weight infants is associated with higher blood amino acid concentrations and improved growth","volume":"18","author":"Haag","year":"2017","journal-title":"Clin. Nutr. ESPEN"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1203\/00006450-198505000-00008","article-title":"Growth velocity and plasma amino acids in the newborn","volume":"19","author":"Scott","year":"1985","journal-title":"Pediatr. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.jpeds.2014.10.021","article-title":"Lipidomic analyses, breast- and formula-feeding, and growth in infants","volume":"166","author":"Prentice","year":"2015","journal-title":"J. Pediatr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11306-014-0628-z","article-title":"The development and validation of a fast and robust dried blood spot based lipid profiling method to study infant metabolism","volume":"10","author":"Koulman","year":"2014","journal-title":"Metabolomics"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.1080\/10408398.2013.803951","article-title":"Phospholipids in Human Milk and Infant Formulas: Benefits and Needs for Correct Infant Nutrition","volume":"56","author":"Cilla","year":"2016","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"pii: 170069","DOI":"10.1098\/rsob.170069","article-title":"Sphingolipids: Membrane microdomains in brain development, function and neurological diseases","volume":"7","author":"Olsen","year":"2017","journal-title":"Open Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.3945\/jn.114.199828","article-title":"Early Supplementation of Phospholipids and Gangliosides Affects Brain and Cognitive Development in Neonatal Piglets Differences in cognitive development: Breast fed ( BF ) vs Formula fed ( FF ) BF children show higher cognitive development scores compa","volume":"2014,","author":"Liu","year":"2014","journal-title":"J. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jpeds.2013.06.032","article-title":"Preterm infant linear growth and adiposity gain: Tradeoffs for later weight status, and IQ","volume":"163","author":"Belfort","year":"2013","journal-title":"J. Pediatr."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.earlhumdev.2008.01.012","article-title":"Postnatal growth failure in preterm infants: Recovery of growth and body composition after term","volume":"84","author":"Roggero","year":"2008","journal-title":"Early Hum. Dev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/nrendo.2015.129","article-title":"Acylcarnitines-old actors auditioning for new roles in metabolic physiology","volume":"11","author":"McCoin","year":"2015","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Luo, L., Aubrecht, J., Li, D., Warner, R.L., Johnson, K.J., Kenny, J., and Colangelo, J.L. (2018). Assessment of serum bile acid profiles as biomarkers of liver injury and liver disease in humans. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193824"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.clp.2016.01.012","article-title":"Cholestasis in Preterm Infants","volume":"43","author":"Satrom","year":"2016","journal-title":"Clin. Perinatol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1097\/MOG.0000000000000057","article-title":"Bile acids and the gut microbiome","volume":"30","author":"Ridlon","year":"2014","journal-title":"Curr. Opin. Gastroenterol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/4\/1188\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:23:36Z","timestamp":1760361816000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/4\/1188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,23]]},"references-count":54,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["nu12041188"],"URL":"https:\/\/doi.org\/10.3390\/nu12041188","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,23]]}}}