{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:00:40Z","timestamp":1791907240464,"version":"4.3.4"},"reference-count":70,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACYT)","award":["Basic Science Projects, Convocation 2011-02, Project No. 167769"],"award-info":[{"award-number":["Basic Science Projects, Convocation 2011-02, Project No. 167769"]}]},{"name":"Fund for Research Strengthening of the Autonomous University of Queretaro","award":["Project FCQ-2018-08 FOFI-UAQ-2018"],"award-info":[{"award-number":["Project FCQ-2018-08 FOFI-UAQ-2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Osteoporosis is a disease of the skeletal system characterized by low bone mass and bone weakening, which increase the risk of fracture. This disease is associated with menopause because hypoestrogenism induces the maturation and activation of osteoclasts. In addition, a low dietary intake of calcium leads to low bone mineral density and postmenopausal osteoporosis. The objectives of this work were to determine calcium bioavailability of Opuntia ficus-indica cladodes at a late maturity stage and to assess its contribution in improving bone health in an ovariectomized rat model. Two-month-old Wistar female rats (n = 35) were used and distributed in seven experimental groups: (i) control group (Crtl), (ii) sham group (SH), (iii) ovariectomized group (OVX), (iv) ovariectomized group supplemented with calcium citrate (CCa), (v) ovariectomized group supplemented with O. ficus-indica powder (NI), (vi) ovariectomized group supplemented with soluble fiber from O. ficus-indica (FS) and (vii) ovariectomized group supplemented with insoluble fiber from O. ficus-indica (FI). Our results showed that calcium in the soluble fiber of O. ficus-indica is bioavailable and contributes to improve the physical, densitometric, biomechanical and microstructural properties of bones in ovariectomized rats. These findings indicated that O. ficus-indica cladodes at a late maturity stage represent a good source of bioavailable calcium and consumption of these cladodes might be beneficial for the prevention of osteoporosis and other bone diseases.<\/jats:p>","DOI":"10.3390\/nu12051431","type":"journal-article","created":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T10:53:59Z","timestamp":1589540039000},"page":"1431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Calcium Bioavailability of Opuntia ficus-indica Cladodes in an Ovariectomized Rat Model of Postmenopausal Bone Loss"],"prefix":"10.3390","volume":"12","author":[{"given":"Michelle","family":"Quintero-Garc\u00eda","sequence":"first","affiliation":[{"name":"Programa de Maestr\u00eda en Ciencias Qu\u00edmico Biol\u00f3gicas, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Cerro de las Campanas S\/N, Quer\u00e9taro C.P. 76010, Mexico"},{"name":"Laboratorio de Qu\u00edmica Medicinal, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Cerro de las Campanas S\/N, Quer\u00e9taro C.P. 76010, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Guti\u00e9rrez-Cortez","sequence":"additional","affiliation":[{"name":"Laboratorio de procesos de transformaci\u00f3n y tecnolog\u00edas emergentes en alimentos, Facultad de Estudios Superiores-Cuautitl\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Km 2.5 Carretera Cuautitl\u00e1n\u2013Teoloyucan, San Sebasti\u00e1n Xhala, Cuautitl\u00e1n-Izcalli C.P. 54714, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6131-9013","authenticated-orcid":false,"given":"Alejandra","family":"Rojas-Molina","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica Medicinal, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Cerro de las Campanas S\/N, Quer\u00e9taro C.P. 76010, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monsserrat","family":"Mendoza-\u00c1vila","sequence":"additional","affiliation":[{"name":"Programa de Maestr\u00eda en Ciencias de la Nutrici\u00f3n Humana, Facultad de Ciencias Naturales, Universidad Aut\u00f3noma de Quer\u00e9taro, Av. de las Ciencias S\/N, Juriquilla C.P. 76230, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8647-5474","authenticated-orcid":false,"given":"Alicia","family":"Del Real","sequence":"additional","affiliation":[{"name":"Centro de F\u00edsica Aplicada y Tecnolog\u00eda Avanzada, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Juriquilla C.P. 7600, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2300-8529","authenticated-orcid":false,"given":"Efra\u00edn","family":"Rubio","sequence":"additional","affiliation":[{"name":"Centro Universitario de Vinculaci\u00f3n y Transferencia de Tecnolog\u00eda, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla, Centro Universitario, Col. San Manuel S\/N, Puebla C.P. 72540, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6275-0072","authenticated-orcid":false,"given":"Daniel","family":"Jim\u00e9nez-Mendoza","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda F\u00edsica, Divisi\u00f3n de Ciencias e Ingenier\u00edas, Universidad de Guanajuato, Campus Le\u00f3n, Lomas del Bosque 103, Col. Lomas del Campestre, Le\u00f3n C.P. 37150, Guanajuato, Mexico"},{"name":"Departamento de Ingenier\u00eda Electromec\u00e1nica, Tecnol\u00f3gico Nacional de M\u00e9xico\/ITS de Pur\u00edsima del Rinc\u00f3n. Blvd. Del Valle 2301, Col. Guardarrayas, Pur\u00edsima del Rinc\u00f3n C.P. 36413, Guanajuato, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isela","family":"Rojas-Molina","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica Medicinal, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Cerro de las Campanas S\/N, Quer\u00e9taro C.P. 76010, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hodges, J.K., Cao, S., Cladis, D.P., and Weaver, C.M. (2019). Lactose intolerance and Bone Health: The Challenge of Ensuring Adequate Calcium Intake. Nutrients, 11.","DOI":"10.3390\/nu11040718"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.bone.2019.04.005","article-title":"The genetic architecture of osteoporosis and fracture risk","volume":"126","author":"Trajanoska","year":"2019","journal-title":"Bone"},{"key":"ref_3","first-page":"135","article-title":"Consideraciones epidemiol\u00f3gicas de las fracturas del f\u00e9mur proximal","volume":"8","author":"Nieto","year":"2012","journal-title":"Mediagraphic"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1002\/jbmr.262","article-title":"The unitary model for estrogen deficiency and the pathogenesis osteoporosis: Is a revision needed?","volume":"26","author":"Khosla","year":"2011","journal-title":"J. Bone Miner. Res."},{"key":"ref_5","first-page":"4","article-title":"American Association of Clinical Endocrinologists Medical Guidelines for Clinical Practice for the Diagnosis and Treatment of Postmenopausal Osteoporosis","volume":"16","author":"Watts","year":"2010","journal-title":"Endocr. Pract."},{"key":"ref_6","first-page":"162","article-title":"An overview on the treatment of postmenopausal osteoporosis","volume":"58","year":"2014","journal-title":"J. Braz. Soc. Endocr. Metabolism."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1016\/j.clinthera.2015.06.006","article-title":"The pathophysiology and treatment of Osteoporosis","volume":"37","author":"Drake","year":"2015","journal-title":"Clin. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Becerra, E., Mendoza-\u00c1vila, M., Jim\u00e9nez-Mendoza, D., Guti\u00e9rrez-Cortez, E., Rodr\u00edguez-Garc\u00eda, M.E., and Rojas-Molina, J.I. (2019). Effect of Nopal (Opuntia ficus indica) consumption at different maturity stages as an only calcium source on bone mineral metabolism in growing rats. Biol. Trace Elem. Res.","DOI":"10.1007\/s12011-019-01752-0"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Becerra, E., Guti\u00e9rrez-Cortez, E., Del Real, A., Rojas-Molina, A., Rodr\u00edguez-Garc\u00eda, M., Rubio, E., Quintero-Garc\u00eda, M., and Rojas-Molina, I. (2017). Bone mineral density, mechanical, microstructural properties and mineral content of the femur in growing rats fed with Opuntia ficus indica as calcium source in diet. Nutrients, 9.","DOI":"10.3390\/nu9020108"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rojas-Molina, I., Guti\u00e9rrez-Cortez, E., Bah, M., Rojas-Molina, A., Ibarra-Alvarado, C., Rivera-Mu\u00f1oz, E., Del Real, A., and Aguilera-Barreiro, M.A. (2015). Characterization of Calcium Compounds in Opuntia ficus indica as a Source of Calcium for Human Diet. J. Chem.","DOI":"10.1155\/2015\/710328"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jfca.2010.03.028","article-title":"Evaluation of oxalates and calcium in nopal pads (Opuntia ficus-indica var. redonda) at different maturity stages","volume":"24","year":"2011","journal-title":"J. Food Compos. Anal."},{"key":"ref_12","unstructured":"(2000). Official Methods of Analysis of the Association of Official Analytical Chemists, AOAC International. [17th ed.]."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1093\/jn\/123.11.1939","article-title":"AIN-93 Purified diets for laboratory rodents, final report of the American Institute of Nutrition ad hoc writing committee on reformulation of the AIN-76A rodent diet","volume":"123","author":"Reeves","year":"1993","journal-title":"J. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Pomeranz, Y., and Meloan, C. (1994). Carbohydrates. Food Analysis. Theory and Practice, Chapman & Hall Inc.. [3rd ed.].","DOI":"10.1007\/978-1-4615-6998-5_36"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1089\/jmf.2011.2047","article-title":"Ultrafine Angelica gigas powder normalizes ovarian hormone levels and has antiosteoporosis properties in ovariectomized rats: Particle size effect","volume":"15","author":"Choi","year":"2012","journal-title":"J. Med. Food."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1248\/bpb.29.291","article-title":"Effects of Fructus Ligustri Lucidi extract on bone turnover and calcium balance in ovariectomized rats","volume":"29","author":"Zhang","year":"2006","journal-title":"Biol. Pharm. Bull."},{"key":"ref_17","first-page":"339","article-title":"Nondigestible oligosaccharides increase calcium absorption and supress bone resorption in ovariectomized rats","volume":"17","author":"Zafar","year":"2003","journal-title":"Nutr. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"125105","DOI":"10.1063\/1.3666864","article-title":"Single x-ray transmission system for bone mineral density determination","volume":"82","year":"2011","journal-title":"Rev. Sci. Instrum."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1089\/jmf.2013.0137","article-title":"Agave fructans, their effect on mineral absorption and bone mineral content","volume":"17","year":"2014","journal-title":"J. Med. Food."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"292","DOI":"10.3390\/ijms10010292","article-title":"Determination of mineral content in methanolic safflower (Carthamus tinctorius L.) seed extract and its effect on osteoblast markers","volume":"10","author":"Lee","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"ref_21","unstructured":"National Institutes of Health (2018, September 24). Calcium: Dietary Supplement Fact Sheet. Office of Dietary Supplements, Available online: https:\/\/ods.od.nih.gov\/factsheets\/Calcium-HealthProfessional\/."},{"key":"ref_22","first-page":"7","article-title":"The aged rat model of ovarian hormone deficiency bone loss","volume":"124","author":"Dike","year":"1988","journal-title":"Endocrinology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1210\/endo.143.2.8614","article-title":"Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: Involvement of both PKA and PKC","volume":"2","author":"Liu","year":"2002","journal-title":"Endocrinology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1681\/ASN.2008121258","article-title":"Indirect regulation of PTH by estrogens may require FGF23","volume":"20","year":"2009","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"9215","DOI":"10.4238\/2015.August.10.1","article-title":"Opg\/Rankl mRNA Dynamic expression in the bone tissue of ovariectomized rats with osteoporosis","volume":"14","author":"Li","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s00223-017-0339-3","article-title":"Prebiotics, bone and mineral metabolism","volume":"102","author":"Whisner","year":"2018","journal-title":"Calcif. Tissue Int."},{"key":"ref_27","first-page":"333","article-title":"Prebiotics and their effect on calcium bioavailability","volume":"24","author":"Lavanda","year":"2011","journal-title":"Nutr. J."},{"key":"ref_28","first-page":"61","article-title":"La fibra diet\u00e9tica","volume":"21","author":"Escudero","year":"2006","journal-title":"Nutr. Hosp."},{"key":"ref_29","first-page":"21","article-title":"Prebiotics and calcium bioavailability","volume":"4","author":"Cashman","year":"2003","journal-title":"Curr. Issues Intest. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00394-017-1445-8","article-title":"Gut microbiota functions: Metabolism of nutrients and other food components","volume":"57","author":"Rowland","year":"2018","journal-title":"Eur. Eur. J. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Pacifici, R. (2018). Bone remodeling and the microbiome. Cold Spring Harb. Perspect. Med.","DOI":"10.1101\/cshperspect.a031203"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1211\/jpp.59.11.0004","article-title":"Determination of calcium salt solubility with changes in pH and P(CO2) simulating varying gastrointestinal environments","volume":"59","author":"Goss","year":"2007","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Wang, H., Bua, P., and Capodice, J. (2014). A comparative study of calcium absorption following a single serving administration of calcium carbonate powder versus calcium citrate tablets in healthy premenopausal women. Food. Nutr. Res.","DOI":"10.3402\/fnr.v58.23229"},{"key":"ref_34","first-page":"85","article-title":"Fisiopatolog\u00eda de la osteoporosis y mecanismo de acci\u00f3n de la PTH","volume":"2","year":"2010","journal-title":"Rev. Osteoporos. Metab. Miner."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1079\/NRR200479","article-title":"Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics","volume":"17","author":"Gibson","year":"2004","journal-title":"Nutr. Res. Rev."},{"key":"ref_36","first-page":"101","article-title":"The effects of dietary supplements of calcium, vitamin D and estrogen hormone on serum levels of OPG and RANKL cytokines and their relationship with increased bone density in rats","volume":"10","author":"Piri","year":"2016","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1681\/ASN.2007010068","article-title":"How fibroblast growth factor 23 works","volume":"18","author":"Liu","year":"2007","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wetmore, J.B. (2011). The link between estrogen and fibroblast growth factor 23. Am. J. Kidney Dis.","DOI":"10.1053\/j.ajkd.2011.08.012"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1021\/jf035332c","article-title":"Oxalate reduces calcium availability in the pads of the Prickly Pear cactus through formation of calcium oxalate crystals","volume":"52","author":"McConn","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_40","first-page":"53","article-title":"Bioavailability and solubility of different calcium-salts as a basis for calcium enrichment of beverages","volume":"1","author":"Kressel","year":"2010","journal-title":"J. Food Nutr. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"43","DOI":"10.4321\/S1889-836X2013000100008","article-title":"Biomecc\u00e1nica y hueso (I): Conceptos b\u00e1sicos y ensayos mec\u00e1nicos cl\u00e1sicos","volume":"5","author":"Guede","year":"2013","journal-title":"Rev. Osteoporos. Metab. Miner."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"131","DOI":"10.2215\/CJN.04151206","article-title":"Normal bone anatomy and phisiology","volume":"3","author":"Clarke","year":"2008","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_43","unstructured":"Martin, B.R., Mohammad, S., and Legette, L. (2009). Diet calcium level but not calcium supplement particle size affects bone density and mechanical properties in ovariectomized rats. Nutr. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0378-8741(02)00325-2","article-title":"Prevention of bone loss in calcium deficient ovariectomized rats by OST-6, a herbal preparation","volume":"84","author":"Reddy","year":"2003","journal-title":"J. Ethnopharmacol."},{"key":"ref_45","first-page":"128","article-title":"Effects of continuous alendronate treatment on bone mass and mechanical properties in ovariectomized rats: Comparison with pamidronate and etidronate in growing rats","volume":"286","author":"Azuma","year":"1998","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.1271\/bbb.69.1970","article-title":"Reduction in dietary calcium\/phosphorus ratio reduces bone mass and strength in ovariectomized rats enchancing bone turnover","volume":"69","author":"Koshihara","year":"2005","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/biof.5520220107","article-title":"Effect of dietary calcium: Phosphorus ratio on bone mineralization and intestinal calcium absorption in ovariectomized rats","volume":"22","author":"Koshihara","year":"2008","journal-title":"Biofactors"},{"key":"ref_48","first-page":"10893","article-title":"Pycnogenol treatment inhibits bone mineral density los and trabecular deterioration in ovariectomized rats","volume":"8","author":"Huang","year":"2015","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1111\/j.1365-2613.2009.00661.x","article-title":"Ovariectomy-associated changes in bone mineral density and bone marrow haematopoiesis in rats","volume":"90","author":"Zhu","year":"2009","journal-title":"Int. J. Exp. Pathol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/jfpe.12084","article-title":"Extraction and characterization of mucilage from wild species of Opuntia","volume":"37","year":"2014","journal-title":"J. Food Process Eng."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0008-6215(00)85543-3","article-title":"Methylation analysis of the mucilage of Opuntia ficus-indica","volume":"88","author":"McGarvie","year":"1981","journal-title":"Carbohydr. Res."},{"key":"ref_52","unstructured":"Coulston, A.M., Boushey, C.J., and Ferruzzi, M.G. (2013). Intestinal microflora and diet in health. Nutrition in the Prevention and Treatment of Disease, Elsevier, Inc.. [3rd ed.]."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s11914-015-0257-0","article-title":"Diet, gut microbiome, and bone health","volume":"2","author":"Weaver","year":"2015","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_54","first-page":"244","article-title":"The effect of 6\u2032-galactooligosaccharides on bone mineralization of rats adapted to different levels of dietary calcium","volume":"66","author":"Chonan","year":"1996","journal-title":"Int. J. Vitam. Nutr. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1007\/s00394-004-0535-6","article-title":"Diet composition and age determine the effects of inulin-type fructans on intestinal calcium absorption in rat","volume":"44","author":"Rashka","year":"2005","journal-title":"Eur. J. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1002\/jsfa.2618","article-title":"Bioavailability of calcium, magnesium and phosphorus in rats fed probiotic, prebiotic and symbiotic powder follow-up infant formulas and their effect on physiological and nutritional parameters","volume":"86","author":"Ros","year":"2006","journal-title":"J. Sci. Food Agric."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1002\/jsfa.2812","article-title":"Effects of probiotic, prebiotic and symbiotic follow-up infant formulas on large intestine morphology and bone mineralization in rats","volume":"87","author":"Ros","year":"2007","journal-title":"J. Sci. Food Agric."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"6501","DOI":"10.1021\/jf2009777","article-title":"Galactooligosaccharides improve mineral absorption and bone properties in growing rats through gut fermentation","volume":"59","author":"Weaver","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1093\/ajcn\/73.2.459s","article-title":"Effects of prebiotics on mineral metabolism","volume":"73","author":"Schaafsma","year":"2001","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1079\/BJN2002661","article-title":"Effect of oligofructose or dietary calcium on repeated calcium and phosphorus balances, bone mineralization and trabecular structure in ovariectomized rats","volume":"88","author":"Acil","year":"2002","journal-title":"Br. J. Nutr."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/jbmr.2388","article-title":"Trabecular and cortical microstructure and fragility of the distal radius women","volume":"30","author":"Bala","year":"2015","journal-title":"J. Bone. Miner. Res."},{"key":"ref_62","unstructured":"National Osteoporosis Foundation (2019, November 12). Balancing the Benefits and Risks of Osteoporosis Treatment. Available online: https:\/\/www.nof.org\/preventing-fractures\/nutrition-for-bone-health\/webinar-series\/."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7762\/cnr.2015.4.1.1","article-title":"The role of calcium in human aging","volume":"4","author":"Beto","year":"2015","journal-title":"Clin. Nutr. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1272\/jnms.77.4","article-title":"The mechanism of mineralization and the role of alkaline phosphatase in health and disease","volume":"77","author":"Orimo","year":"2010","journal-title":"J. Nippon. Med. Sch."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1515\/jbcr-2017-0006","article-title":"Changes of serum concentrations of alkaline phosphatase and metalloproteinase-9 in ovariectomized wistar rat model of osteoporosis","volume":"10","author":"Grigoryan","year":"2017","journal-title":"J. Biomed. Clin. Res."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Wheater, G., Elshahaly, M., Tuck, S.P., Datta, H.K., and Laar, J.M. (2013). The clinical utility of bone marker measurements in osteoporosis. J. Transl. Med.","DOI":"10.1186\/1479-5876-11-201"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Patti, A., Gennari, L., Merlotti, D., and Dotta, F. (2013). Endocrine actions of osteocalcin. Int. J. Endocrinol.","DOI":"10.1155\/2013\/846480"},{"key":"ref_68","first-page":"1","article-title":"Serum osteocalcin levels and bone mineral density in ovariectomized rats","volume":"5","author":"Ce","year":"2014","journal-title":"Int. J. Innov. Sci. Eng. Technol."},{"key":"ref_69","unstructured":"Usha, K., and Nandeesh, B.N. (2012). Physiology of Bone Formation Remodeling, and Metabolism, Springer."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1007\/s00264-015-2926-0","article-title":"Collagen type I coating stimulates bone regeneration and osteointegration of titanium implants in the osteopenic rat","volume":"39","author":"Sartori","year":"2015","journal-title":"Int. Orthop."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/5\/1431\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:29:04Z","timestamp":1760174944000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/5\/1431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,15]]},"references-count":70,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["nu12051431"],"URL":"https:\/\/doi.org\/10.3390\/nu12051431","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,15]]}}}