{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:50:56Z","timestamp":1788375056569,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T00:00:00Z","timestamp":1590019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>\u03b1-Casozepine (\u03b1-CZP) is an anxiolytic-like bioactive decapeptide derived from bovine \u03b1s1-casein. The N-terminal peptide YLGYL was previously identified after proteolysis of the original peptide in an in vitro digestion model. Its putative anxiolytic-like properties were evaluated in a Swiss mice model using a light\/dark box (LDB) after an intraperitoneal injection (0.5 mg\/kg). The effect of YLGYL on c-Fos expression in brain regions linked to anxiety regulation was afterwards evaluated via immunofluorescence and compared to those of \u03b1-CZP and diazepam, a reference anxiolytic benzodiazepine. YLGYL elicited some anxiolytic-like properties in the LDB, similar to \u03b1-CZP and diazepam. The two peptides displayed some strong differences compared with diazepam in terms of c-Fos expression modulation in the prefontal cortex, the amygdala, the nucleus of the tractus solitarius, the periaqueductal grey, and the raphe magnus nucleus, implying a potentially different mode of action. Additionally, YLGYL modulated c-Fos expression in the amygdala and in one of the raphe nuclei, displaying a somewhat similar pattern of activation as \u03b1-CZP. Nevertheless, some differences were also spotted between the two peptides, making it possible to formulate the hypothesis that these peptides could act differently on anxiety regulation. Taken together, these results showed that YLGYL could contribute to the in vivo overall action of \u03b1-CZP.<\/jats:p>","DOI":"10.3390\/nu12051497","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T11:31:18Z","timestamp":1590060678000},"page":"1497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Anxiolytic Activity and Brain Modulation Pattern of the \u03b1-Casozepine-Derived Pentapeptide YLGYL in Mice"],"prefix":"10.3390","volume":"12","author":[{"given":"Simon","family":"Benoit","sequence":"first","affiliation":[{"name":"INRAE, URAFPA, Universit\u00e9 de Lorraine, F-54000 Nancy, France"},{"name":"UMR PNCA, AgroParisTech, Inrae, Universit\u00e9 Paris-Saclay, F-75231 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Chaumontet","sequence":"additional","affiliation":[{"name":"UMR PNCA, AgroParisTech, Inrae, Universit\u00e9 Paris-Saclay, F-75231 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Ingredia, F-62000 Arras, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Cakir-Kiefer","sequence":"additional","affiliation":[{"name":"INRAE, URAFPA, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Boulier","sequence":"additional","affiliation":[{"name":"Ingredia, F-62000 Arras, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Tom\u00e9","sequence":"additional","affiliation":[{"name":"UMR PNCA, AgroParisTech, Inrae, Universit\u00e9 Paris-Saclay, F-75231 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6010-611X","authenticated-orcid":false,"given":"Laurent","family":"Miclo","sequence":"additional","affiliation":[{"name":"INRAE, URAFPA, Universit\u00e9 de Lorraine, F-54000 Nancy, France"},{"name":"CALBINOTOX, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1096\/fj.00-0685fje","article-title":"Characterization of alpha-casozepine, a tryptic peptide from bovine alpha(s1)-casein with benzodiazepine-like activity","volume":"15","author":"Miclo","year":"2001","journal-title":"FASEB J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/j.1472-8206.2009.00672.x","article-title":"Anxiolytic-like effects and safety profile of a tryptic hydrolysate from bovine alpha s1-casein in rats","volume":"23","author":"Messaoudi","year":"2009","journal-title":"Fundam. 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