{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:07:14Z","timestamp":1782742034699,"version":"3.54.5"},"reference-count":57,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T00:00:00Z","timestamp":1590019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Serum high-density lipoprotein cholesterol (HDL-C) levels and cholesterol excretion are closely associated with the risk of cardiovascular complications. The specific aim of the present study was to investigate the cholesterol lowering effect of mulberry fruit in rats fed a high cholesterol\/cholic acid diet. Four-week supplementation with mulberry fruit extract significantly decreased serum and hepatic cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-C), and fecal bile acid levels without changes in body weight and food intake (p &lt; 0.05). Mulberry fruit extract significantly inhibited hepatic sterol-regulatory element binding protein (Srebp) 2 gene expression and upregulated hepatic mRNA levels of liver X receptor alpha (Lxr-\u03b1), ATP-binding cassette transporter 5 (Abcg5), and cholesterol 7 alpha-hydroxylase (Cyp7a1), which are involved in hepatic bile acid synthesis and cholesterol metabolism (p &lt; 0.05). In addition, hepatic microRNA-33 expression was significantly inhibited by supplementation of mulberry fruit extract (p &lt; 0.05). These results suggest the involvement of miR-33, its associated hepatic bile acid synthesis, HDL formation, and cholesterol metabolism in mulberry fruit-mediated beneficial effects on serum and hepatic lipid abnormalities.<\/jats:p>","DOI":"10.3390\/nu12051499","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T11:31:18Z","timestamp":1590060678000},"page":"1499","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Mulberry Fruit Extract Promotes Serum HDL-Cholesterol Levels and Suppresses Hepatic microRNA-33 Expression in Rats Fed High Cholesterol\/Cholic Acid Diet"],"prefix":"10.3390","volume":"12","author":[{"given":"Soojin","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mak-Soon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6949-7666","authenticated-orcid":false,"given":"Eugene","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonjin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaerin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6266-5460","authenticated-orcid":false,"given":"Chong-Tai","family":"Kim","sequence":"additional","affiliation":[{"name":"R&amp;D Center, EastHill Corporation, Gwonseon-gu, Suwon-si, Gyeonggi-do 16642, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In-Hwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Integrated Biomedical and Life Sciences, Korea University, Seoul 02841, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7280-7597","authenticated-orcid":false,"given":"Yangha","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.arcmed.2015.06.006","article-title":"Global Overview of the Epidemiology of Atherosclerotic Cardiovascular Disease","volume":"46","author":"Barquera","year":"2015","journal-title":"Arch. Med. Res."},{"key":"ref_2","unstructured":"World Health Organization (2016). Hearts: Technical Package for Cardiovascular Disease Management in Primary Health Care, WHO."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1016\/j.jacc.2013.11.002","article-title":"2013 ACC\/AHA guideline on the treatment of blood cholesterol to reduce atherosclerotic cardiovascular risk in adults: A report of the American College of Cardiology\/American Heart Association Task Force on Practice Guidelines","volume":"63","author":"Stone","year":"2014","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1056\/NEJM198911093211907","article-title":"High-density lipoprotein\u2014The clinical implications of recent studies","volume":"321","author":"Gordon","year":"1989","journal-title":"N. Engl. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1056\/NEJMoa064278","article-title":"HDL cholesterol, very low levels of LDL cholesterol, and cardiovascular events","volume":"357","author":"Barter","year":"2007","journal-title":"N. Engl. J. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1161\/CIRCRESAHA.114.300632","article-title":"Regulation of high-density lipoprotein metabolism","volume":"114","author":"Rye","year":"2014","journal-title":"Circ. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/nrcardio.2015.124","article-title":"Dysfunctional HDL and atherosclerotic cardiovascular disease","volume":"13","author":"Rosenson","year":"2016","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.mam.2014.02.001","article-title":"Cross-talk between liver and intestine in control of cholesterol and energy homeostasis","volume":"37","author":"Groen","year":"2014","journal-title":"Mol. Asp. Med."},{"key":"ref_9","first-page":"333","article-title":"Srebp2: A master regulator of sterol and fatty acid synthesis","volume":"57","author":"Madison","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"18793","DOI":"10.1074\/jbc.M109927200","article-title":"Regulation of ATP-binding cassette sterol transporters ABCG5 and ABCG8 by the liver X receptors alpha and beta","volume":"277","author":"Repa","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1126\/science.1168974","article-title":"LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor","volume":"325","author":"Zelcer","year":"2009","journal-title":"Science"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/S0092-8674(00)81432-4","article-title":"Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR alpha","volume":"93","author":"Peet","year":"1998","journal-title":"Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"939","DOI":"10.3389\/fphar.2018.00939","article-title":"Bile Acid Synthesis: From Nature to the Chemical Modification and Synthesis and Their Applications as Drugs and Nutrients","volume":"9","author":"Sarenac","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1007\/s10354-018-0626-2","article-title":"Cholesterol metabolism-physiological regulation and pathophysiological deregulation by the endoplasmic reticulum","volume":"168","author":"Rohrl","year":"2018","journal-title":"Wien. Med. Wochenschr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1152\/physrev.00005.2005","article-title":"ATP-binding cassette transporter A1: A cell cholesterol exporter that protects against cardiovascular disease","volume":"85","author":"Oram","year":"2005","journal-title":"Physiol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1161\/ATVBAHA.112.301195","article-title":"ABCA1 mediates unfolding of apolipoprotein AI N terminus on the cell surface before lipidation and release of nascent high-density lipoprotein","volume":"33","author":"Wang","year":"2013","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1074\/jbc.271.4.2023","article-title":"Regulation of cellular cholesterol efflux by lecithin:cholesterol acyltransferase reaction through nonspecific lipid exchange","volume":"271","author":"Czarnecka","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"10383","DOI":"10.1021\/acs.jafc.7b03614","article-title":"The Mulberry (Morus alba L.) Fruit\u2014A Review of Characteristic Components and Health Benefits","volume":"65","author":"Yuan","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1594963","DOI":"10.1155\/2017\/1594963","article-title":"Mulberry Fruit Extract Affords Protection against Ethyl Carbamate-Induced Cytotoxicity and Oxidative Stress","volume":"2017","author":"Chen","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1089\/jmf.2016.3665","article-title":"Mulberry (Morus alba L.) Fruit Extract Containing Anthocyanins Improves Glycemic Control and Insulin Sensitivity via Activation of AMP-Activated Protein Kinase in Diabetic C57BL\/Ksj-db\/db Mice","volume":"19","author":"Choi","year":"2016","journal-title":"J. Med. Food"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Jung, S., Lee, M.S., Choi, A.J., Kim, C.T., and Kim, Y. (2019). Anti-Inflammatory Effects of High Hydrostatic Pressure Extract of Mulberry (Morus alba) Fruit on LPS-Stimulated RAW264.7 Cells. Molecules, 24.","DOI":"10.3390\/molecules24071425"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"249","DOI":"10.5338\/KJEA.2017.36.4.39","article-title":"Quali-quantitative analysis of flavonoids for mulberry leaf and fruit of \u2018Suhyang\u2019","volume":"36","author":"Ju","year":"2017","journal-title":"Korean J. Environ. Agric."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1080\/09168451.2017.1281723","article-title":"Food processing by high hydrostatic pressure","volume":"81","author":"Yamamoto","year":"2017","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1177\/1082013216659610","article-title":"Effects of high hydrostatic pressure and thermal processing on bioactive compounds, antioxidant activity, and volatile profile of mulberry juice","volume":"23","author":"Wang","year":"2017","journal-title":"Food Sci. Technol. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1093\/clinchem\/18.6.499","article-title":"Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge","volume":"18","author":"Friedewald","year":"1972","journal-title":"Clin. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1373\/clinchem.2016.257725","article-title":"The Changing Face of HDL and the Best Way to Measure It","volume":"63","author":"Karathanasis","year":"2017","journal-title":"Clin. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.foodchem.2017.09.106","article-title":"Comparison of the protective effect of black and white mulberry against ethyl carbamate-induced cytotoxicity and oxidative damage","volume":"243","author":"Li","year":"2018","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.intimp.2013.03.032","article-title":"Anti-inflammatory effects of phenolic extracts from strawberry and mulberry fruits on cytokine secretion profiles using mouse primary splenocytes and peritoneal macrophages","volume":"16","author":"Liu","year":"2013","journal-title":"Int. Immunopharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"498","DOI":"10.3746\/jkfn.2014.43.4.498","article-title":"Effect of mulberry (Morus alba L.) extract on blood flow improvement","volume":"43","author":"Park","year":"2014","journal-title":"J. Korean Soc. Food Sci. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jep.2017.02.037","article-title":"Effects of the ethanol extract of black mulberry (Morus nigra L.) fruit on experimental atherosclerosis in rats","volume":"200","author":"Jiang","year":"2017","journal-title":"J. Ethnopharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7605","DOI":"10.1021\/jf9014697","article-title":"Effects of mulberry (Morus alba L.) extracts on lipid homeostasis in vitro and in vivo","volume":"57","author":"Liu","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jnutbio.2016.07.004","article-title":"Mulberry anthocyanin extract ameliorates insulin resistance by regulating PI3K\/AKT pathway in HepG2 cells and db\/db mice","volume":"36","author":"Yan","year":"2016","journal-title":"J. Nutr. Biochem."},{"key":"ref_35","first-page":"133","article-title":"Effect of pomegranate flower extract on cisplatin-induced nephrotoxicity in rats","volume":"3","author":"Motamedi","year":"2014","journal-title":"J. Nephropathol."},{"key":"ref_36","first-page":"373","article-title":"Hypolipidemic and antioxidant effects of black tea extract and quercetin in atherosclerotic rats","volume":"7","author":"Widowati","year":"2013","journal-title":"Int. J. Med. Pharm. Sci. Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1111\/bcp.12923","article-title":"Antioxidant and antiplatelet activity by polyphenol-rich nutrients: Focus on extra virgin olive oil and cocoa","volume":"83","author":"Loffredo","year":"2017","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5177","DOI":"10.2174\/1381612811319290003","article-title":"Cardiovascular and systemic risk in nonalcoholic fatty liver disease\u2014Atherosclerosis as a major player in the natural course of NAFLD","volume":"19","author":"Lonardo","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/S1097-2765(00)00050-2","article-title":"Molecular basis for feedback regulation of bile acid synthesis by nuclear receptors","volume":"6","author":"Lu","year":"2000","journal-title":"Mol. Cell."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"12526","DOI":"10.1074\/jbc.273.20.12526","article-title":"Differential transcriptional regulation of the human squalene synthase gene by sterol regulatory element-binding proteins (SREBP) 1a and 2 and involvement of 5\u2032 DNA sequence elements in the regulation","volume":"273","author":"Guan","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"16237","DOI":"10.1073\/pnas.252582399","article-title":"Disruption of Abcg5 and Abcg8 in mice reveals their crucial role in biliary cholesterol secretion","volume":"99","author":"Yu","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1172\/JCI0215387","article-title":"Human cholesterol 7alpha-hydroxylase (CYP7A1) deficiency has a hypercholesterolemic phenotype","volume":"110","author":"Pullinger","year":"2002","journal-title":"J. Clin. Investig."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1002\/hep.24107","article-title":"Overexpression of cholesterol 7alpha-hydroxylase promotes hepatic bile acid synthesis and secretion and maintains cholesterol homeostasis","volume":"53","author":"Li","year":"2011","journal-title":"Hepatology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2790","DOI":"10.1074\/jbc.M307363200","article-title":"Hepatic overexpression of murine Abcb11 increases hepatobiliary lipid secretion and reduces hepatic steatosis","volume":"279","author":"Figge","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1002\/hep.21212","article-title":"Atp8b1 deficiency in mice reduces resistance of the canalicular membrane to hydrophobic bile salts and impairs bile salt transport","volume":"44","author":"Paulusma","year":"2006","journal-title":"Hepatology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"G1119","DOI":"10.1152\/ajpgi.90371.2008","article-title":"Knockdown of ATP8B1 expression leads to specific downregulation of the bile acid sensor FXR in HepG2 cells: Effect of the FXR agonist GW4064","volume":"296","author":"Hierro","year":"2009","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1056\/NEJMoa1409065","article-title":"HDL cholesterol efflux capacity and incident cardiovascular events","volume":"371","author":"Rohatgi","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/S2213-8587(15)00126-6","article-title":"Association of HDL cholesterol efflux capacity with incident coronary heart disease events: A prospective case-control study","volume":"3","author":"Saleheen","year":"2015","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.cca.2009.12.027","article-title":"Regulation of cholesterol homeostasis by liver X receptors","volume":"411","author":"Hu","year":"2010","journal-title":"Clinica Chimica Acta"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1172\/JCI0215748","article-title":"Increased ABCA1 activity protects against atherosclerosis","volume":"110","author":"Singaraja","year":"2002","journal-title":"J. Clin. Investig."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1161\/ATVBAHA.108.182303","article-title":"Tissue-specific roles of ABCA1 influence susceptibility to atherosclerosis","volume":"29","author":"Brunham","year":"2009","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1172\/JCI117412","article-title":"Apolipoprotein AI transgene corrects apolipoprotein E deficiency-induced atherosclerosis in mice","volume":"94","author":"Paszty","year":"1994","journal-title":"J. Clin. Investig."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.bbalip.2011.08.006","article-title":"Increased risk of coronary artery disease in Caucasians with extremely low HDL cholesterol due to mutations in ABCA1, APOA1, and LCAT","volume":"1821","author":"Tietjen","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs: Genomics, biogenesis, mechanism, and function","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"12228","DOI":"10.1073\/pnas.1005191107","article-title":"miR-33 links SREBP-2 induction to repression of sterol transporters","volume":"107","author":"Marquart","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1126\/science.1189123","article-title":"MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis","volume":"328","author":"Kristo","year":"2010","journal-title":"Science"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1126\/science.1189862","article-title":"MiR-33 contributes to the regulation of cholesterol homeostasis","volume":"328","author":"Rayner","year":"2010","journal-title":"Science"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/5\/1499\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:31:12Z","timestamp":1760175072000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/5\/1499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,21]]},"references-count":57,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["nu12051499"],"URL":"https:\/\/doi.org\/10.3390\/nu12051499","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,21]]}}}