{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:45:44Z","timestamp":1791395144718,"version":"4.3.3"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,5,30]],"date-time":"2020-05-30T00:00:00Z","timestamp":1590796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["National Natural Science Foundation of China (No.31571827 and No.31530056)."],"award-info":[{"award-number":["National Natural Science Foundation of China (No.31571827 and No.31530056)."]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Plan","award":["2017YFD0400200"],"award-info":[{"award-number":["2017YFD0400200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Previously, we showed the preventive effects of Lactobacillus plantarum ZS2058 (ZS2058) on Salmonella infection in murine models. In this work, we found that eugenol has a selective antibacterial effect, which inhibited Salmonella more than probiotics ZS2058 in vitro. It suggested a synergistic effect of them beyond their individual anti-Salmonella activity. We verified the conjecture in murine models. The results showed that the combination of ZS2058 and eugenol (CLPZE) significantly increased (p = 0.026) the survival rate of Salmonella-infected mice from 60% to 80% and the effect of CLPZE on preventing Salmonella-infection was 2-fold that of ZS2058 alone and 6-fold that of eugenol alone. CLPZE had a synergistic effect on inhibiting ST growth (the coefficient drug interaction ((CDI) = 0.829), reducing its invasiveness (CDI = 0.373) and downregulating virulence genes\u2019 expression in vitro. CLPZE helped the host form a healthier gut ecosystem. CLPZE also elicited a stronger and earlier immune response to systemic infection. In conclusion, these obtained results suggest that ZS2058 and eugenol have a synergistic effect on preventing Salmonella infection and open new perspectives in the strategies of controlling the prevalence of Salmonella by combination of probiotics and functional food components.<\/jats:p>","DOI":"10.3390\/nu12061611","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T11:49:21Z","timestamp":1591012161000},"page":"1611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Synergistic Effect of Eugenol and Probiotic Lactobacillus Plantarum Zs2058 against Salmonella Infection in C57bl\/6 Mice"],"prefix":"10.3390","volume":"12","author":[{"given":"Fanfen","family":"Song","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junsheng","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxuan","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diangeng","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqin","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1929-2202","authenticated-orcid":false,"given":"Zhennan","family":"Gu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"},{"name":"School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Antillon, M., Warren, J.L., Crawford, F.W., Weinberger, D.M., Kurum, E., Pak, G.D., Marks, F., and Pitzer, V.E. 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