{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T18:31:59Z","timestamp":1784831519234,"version":"3.55.0"},"reference-count":56,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T00:00:00Z","timestamp":1594425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>This study aimed at investigating the anti-obesity and anti-dyslipidemic effects of Stachys sieboldii Miq. root (SS) powder in rats following a high-fat and high-cholesterol (HFC) diet for 6 weeks. Thirty-two Sprague\u2013Dawley rats were fed one of the following diets: a regular diet (RD), HFC, HFC supplemented with 3% SS (HFC + 3SS) or HFC supplemented with 5% SS (HFC + 5SS). Following an HFC diet increased body weight (BW) gain (p &lt; 0.001) and the food efficiency ratio (FER; p &lt; 0.001); however, SS consumption gradually prevented the HFC-induced BW gain (p &lt; 0.001) and increase in FER (p &lt; 0.01). The HFC diet resulted in increased liver size (p &lt; 0.001) and total adipose tissue weight (p &lt; 0.001), whereas the SS supplementation decreased hepatomegaly (p &lt; 0.05) and body fat mass (p &lt; 0.001). SS consumption prevented the increased activities of serum alanine aminotransferase (ALT; p &lt; 0.001), aspartate aminotransferase (AST; p &lt; 0.001), alkaline phosphatase (ALP; p &lt; 0.01 in HFC + 5SS) and lactate dehydrogenase (LDH; p &lt; 0.001 in HFC + 5SS) induced by the HFC diet (p &lt; 0.001). The SS supplementation improved lipid profiles in the circulation by lowering triglyceride (TG; p &lt; 0.01), total cholesterol (TC; p &lt; 0.001) and non-HDL cholesterol (non-HDL-C; p &lt; 0.001) levels, as well as the atherogenic index (p &lt; 0.01) and cardiac risk factor (p &lt; 0.01). The lipid distribution in the liver (p &lt; 0.05) and white adipose tissues (WAT; p &lt; 0.001) of the HFC + SS diet-consuming rats was remarkably lower than that of the HFC diet-consuming rats. The average size of the epididymal adipose tissue (p &lt; 0.001) was significantly lower in the HFC + SS diet-fed rats than in the HFC diet-fed rats. The fecal lipid (&gt;3% SS; p &lt; 0.001) and cholesterol (5% SS; p &lt; 0.001) efflux levels were significantly elevated by the SS supplementation compared to those measured in the RD or HFC diet-fed groups. In addition, the hepatic lipid and cholesterol metabolism-related gene expressions were affected by SS consumption, as the hepatic anabolic gene expression (Acc; p &lt; 0.001, Fas; p &lt; 0.001 and G6pdh; p &lt; 0.01) was significantly attenuated. The HFC + 5SS diet-fed rats exhibited elevated hepatic Cyp7a1 (p &lt; 0.001), Hmgcr (p &lt; 0.001) and Ldlr (p &lt; 0.001) mRNA expression levels compared to the HFC diet-fed rats. These results suggest that SS may possess anti-adipogenic and lipid-lowering effects by enhancing lipid and cholesterol efflux in mammals.<\/jats:p>","DOI":"10.3390\/nu12072063","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T04:46:01Z","timestamp":1594701961000},"page":"2063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Stachys sieboldii Miq. Root Attenuates Weight Gain and Dyslipidemia in Rats on a High-Fat and High-Cholesterol Diet"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2282-2131","authenticated-orcid":false,"given":"Jennifer K.","family":"Lee","sequence":"first","affiliation":[{"name":"Food Science &amp; Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5737-612X","authenticated-orcid":false,"given":"Jae-Joon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon-Kyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8542-8724","authenticated-orcid":false,"given":"Youngseung","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Center for Industrialization of Natural Neutralization, Dankook University, Cheonan 31116, Korea"},{"name":"Department of Food Science and Nutrition, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5282-531X","authenticated-orcid":false,"given":"Jung-Heun","family":"Ha","sequence":"additional","affiliation":[{"name":"Research Center for Industrialization of Natural Neutralization, Dankook University, Cheonan 31116, Korea"},{"name":"Department of Food Science and Nutrition, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,11]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (2020, June 11). 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