{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:59:24Z","timestamp":1787029164332,"version":"build-2736575974"},"reference-count":85,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T00:00:00Z","timestamp":1598227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The inclusion of pulses in traditional wheat-based food products is increasing as the food industry and consumers are recognizing the nutritional benefits due to the high protein, antioxidant activity, and good source of dietary fiber of pulses. Iron deficiency is a significant global health challenge, affecting approximately 30% of the world\u2019s population. Dietary iron deficiency is the foremost cause of anemia, a condition that harms cognitive development and increases maternal and infant mortality. This study intended to demonstrate the potential efficacy of low-phytate biofortified pea varieties on dietary iron (Fe) bioavailability, as well as on intestinal microbiome, energetic status, and brush border membrane (BBM) functionality in vivo (Gallus gallus). We hypothesized that the low-phytate biofortified peas would significantly improve Fe bioavailability, BBM functionality, and the prevalence of beneficial bacterial populations. A six-week efficacy feeding (n = 12) was conducted to compare four low-phytate biofortified pea diets with control pea diet (CDC Bronco), as well as a no-pea diet. During the feeding trial, hemoglobin (Hb), body-Hb Fe, feed intake, and body weight were monitored. Upon the completion of the study, hepatic Fe and ferritin, pectoral glycogen, duodenal gene expression, and cecum bacterial population analyses were conducted. The results indicated that certain low-phytate pea varieties provided greater Fe bioavailability and moderately improved Fe status, while they also had significant effects on gut microbiota and duodenal brush border membrane functionality. Our findings provide further evidence that the low-phytate pea varieties appear to improve Fe physiological status and gut microbiota in vivo, and they highlight the likelihood that this strategy can further improve the efficacy and safety of the crop biofortification and mineral bioavailability approach.<\/jats:p>","DOI":"10.3390\/nu12092563","type":"journal-article","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T10:37:32Z","timestamp":1598265452000},"page":"2563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Low Phytate Peas (Pisum sativum L.) Improve Iron Status, Gut Microbiome, and Brush Border Membrane Functionality In Vivo (Gallus gallus)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3059-0606","authenticated-orcid":false,"given":"Tom","family":"Warkentin","sequence":"first","affiliation":[{"name":"Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9193-317X","authenticated-orcid":false,"given":"Nikolai","family":"Kolba","sequence":"additional","affiliation":[{"name":"USDA-ARS, Robert W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7856-5197","authenticated-orcid":false,"given":"Elad","family":"Tako","sequence":"additional","affiliation":[{"name":"USDA-ARS, Robert W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S31","DOI":"10.1177\/15648265110321S105","article-title":"Biofortification: A New Tool to Reduce Micronutrient Malnutrition","volume":"32","author":"Bouis","year":"2011","journal-title":"Food Nutr. Bull."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1177\/15648265050264S309","article-title":"Biotechnology, biofortification, and global health","volume":"26","author":"Welch","year":"2005","journal-title":"Food Nutr. Bull."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.pbi.2008.01.007","article-title":"Biofortified crops to alleviate micronutrient malnutrition","volume":"11","author":"Mayer","year":"2008","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/BF02162964","article-title":"Multielement determination in wheat and bran","volume":"105","author":"Iskander","year":"1986","journal-title":"J. Radioanal. Nucl. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2135\/cropsci2004.4180","article-title":"Identification and characterization of low phytic acid wheat","volume":"44","author":"Guttieri","year":"2004","journal-title":"Crop Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.anifeedsci.2006.04.007","article-title":"Distribution of phytase activity, total phosphorus and phytate phosphorus in legume seeds, cereals and cereal by-products as influenced by harvest year and cultivar","volume":"133","author":"Steiner","year":"2007","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_7","unstructured":"FAOSTAT (2019, January 15). Production. Crops. Available online: http:\/\/www.fao.org\/faostat\/en\/#data\/QC."},{"key":"ref_8","first-page":"141","article-title":"Effect of genotype and environment on the concentrations of starch and protein in, and the physicochemical properties of starch from, field pea and fababean","volume":"92","author":"Warkentin","year":"2011","journal-title":"J. Sci. Food Agric."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1002\/jsfa.7461","article-title":"Chemical composition, nutritional value and in vitro starch digestibility of roasted chickpeas","volume":"96","author":"Simsek","year":"2016","journal-title":"J. Sci. Food Agric."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.1016\/j.foodres.2011.07.006","article-title":"Structure of faba bean, black bean and pinto bean starches at different levels of granule organization and their physicochemical properties","volume":"44","author":"Ambigaipalan","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.foodres.2009.09.004","article-title":"Minor components of pulses and their potential impact on human health","volume":"43","author":"Oomah","year":"2010","journal-title":"Food Res. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.foodres.2009.09.035","article-title":"Carbohydrate composition of raw and extruded pulse flours","volume":"43","author":"Berrios","year":"2010","journal-title":"Food Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1094\/CCHEM-05-10-0077","article-title":"Chemical Composition and Antinutritional Factors of Field Peas (Pisum sativum), Chickpeas (Cicer arietinum), and Faba Beans (Vicia faba) as Affected by Extrusion Preconditioning and Drying Temperatures","volume":"88","author":"Adamidou","year":"2011","journal-title":"Cereal Chem. J."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Pacifici, S., Song, J., Zhang, C., Wang, Q., Glahn, R.P., Kolba, N., and Tako, E. (2017). Intra Amniotic Administration of Raffinose and Stachyose Affects the Intestinal Brush Border Functionality and Alters Gut Microflora Populations. Nutrients, 9.","DOI":"10.3390\/nu9030304"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/tpj.12716","article-title":"Improvement of pea biomass and seed productivity by simultaneous increase of phloem and embryo loading with amino acids","volume":"81","author":"Zhang","year":"2014","journal-title":"Plant J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1093\/mp\/ssp106","article-title":"Phenylpropanoid Biosynthesis","volume":"3","author":"Vogt","year":"2010","journal-title":"Mol. Plant"},{"key":"ref_17","first-page":"15","article-title":"Phenolic compounds of seed coats of white and coloured varieties of pea (Pisum sativum L.) and their total antioxidant activity","volume":"20","author":"Ciska","year":"2011","journal-title":"Czech J. Food Sci."},{"key":"ref_18","first-page":"116","article-title":"Occurrence of phenolic compounds in the seed coat and the cotyledon of peas (Pisum sativum L.)","volume":"219","author":"Estrella","year":"2004","journal-title":"Eur. Food Res. Technol."},{"key":"ref_19","first-page":"27","article-title":"Chemical characteristics and biological functions of phenolic acids of buckwheat and lentil seeds","volume":"12","author":"Zadernowski","year":"1992","journal-title":"Fagopyrum"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.foodchem.2013.11.093","article-title":"Bound phenolics in foods, a review","volume":"152","year":"2014","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1385\/ABAB:133:2:87","article-title":"Feruloyl esterase","volume":"133","author":"Wong","year":"2006","journal-title":"Appl. Biochem. Biotech."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3275S","DOI":"10.1093\/jn\/133.10.3275S","article-title":"Antioxidant Activity of Tea Polyphenols In Vivo: Evidence from Animal Studies","volume":"133","author":"Frei","year":"2003","journal-title":"J. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7981","DOI":"10.1021\/jf071704w","article-title":"Phytochemicals for Health, the Role of Pulses","volume":"55","author":"Rochfort","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1079\/BJN2002721","article-title":"Non-nutrient bioactive substances of pulses","volume":"88","author":"Champ","year":"2002","journal-title":"Brit. J. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/S0031-9422(03)00446-1","article-title":"myo-Inositol-1,2,3,4,5,6-hexakisphosphate","volume":"64","author":"Raboy","year":"2003","journal-title":"Phytochemistry"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1002\/cche.10103","article-title":"Blending studies using wheat and lentil cotyledon flour-Effects on rheology and bread quality","volume":"95","author":"Portman","year":"2018","journal-title":"Cereal Chem. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.foodchem.2017.04.158","article-title":"Effects of chemical composition and baking on in vitro digestibility of proteins in breads made from selected gluten-containing and gluten-free flours","volume":"233","author":"Wu","year":"2017","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1016\/j.clnu.2016.05.002","article-title":"Gluten free diet and nutrient deficiencies: A review","volume":"35","author":"Vici","year":"2016","journal-title":"Clin. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"320","DOI":"10.2135\/cropsci2014.06.0412","article-title":"Iron Bioavailability in Low Phytate Pea","volume":"55","author":"Liu","year":"2015","journal-title":"Crop. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"891","DOI":"10.2135\/cropsci2016.08.0682","article-title":"Effect of iron, phytate and carotenoid concentration on iron bioavailability in field pea seeds","volume":"57","author":"Bangar","year":"2017","journal-title":"Crop Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s00122-010-1305-x","article-title":"Genetic diversity, inter-gene pool introgression and nutritional quality of common beans (Phaseolus vulgaris L.) from Central Africa","volume":"121","author":"Blair","year":"2010","journal-title":"Theor. Appl. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8287","DOI":"10.1021\/jf400774y","article-title":"Mineral Biofortification Strategies for Food Staples: The Example of Common Bean","volume":"61","author":"Blair","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/1475-2891-10-113","article-title":"Biofortified red mottled beans (Phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: Studies in poultry (Gallus gallus) and an in vitro digestion\/Caco-2 model","volume":"10","author":"Tako","year":"2011","journal-title":"Nutr. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1475-2891-12-3","article-title":"High bioavailablilty iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro (Caco-2 model) and in vivo (Gallus gallus)","volume":"12","author":"Tako","year":"2013","journal-title":"Nutr. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"514","DOI":"10.3382\/ps.2009-00326","article-title":"Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability","volume":"89","author":"Tako","year":"2010","journal-title":"Poult. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Dias, D.M., Kolba, N., Binyamin, D., Ziv, O., Regini Nutti, M., Martino, H.S.D., Koren, O., and Tako, E. (2018). Iron Biofortified Carioca Bean (Phaseolus vulgaris L.)-Based Brazilian Diet Delivers More Absorbable Iron and Affects the Gut Microbiota In Vivo (Gallus gallus). Nutrients, 13.","DOI":"10.3390\/nu10121970"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6291","DOI":"10.1021\/acs.jafc.8b01481","article-title":"Alterations in the Gut (Gallus gallus) Microbiota Following the Consumption of Zinc Biofortified Wheat (Triticum aestivum)-Based Diet","volume":"66","author":"Reed","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1021\/acs.jafc.7b04905","article-title":"Linoleic Acid: Dihomo-\u03b3-Linolenic Acid Ratio Predicts the Efficacy of Zn-Biofortified Wheat in Chicken (Gallus gallus)","volume":"66","author":"Knez","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Wiesinger, J.A., Glahn, R., Cichy, K.A., Kolba, N., Hart, J., and Tako, E. (2019). An In Vivo (Gallus gallus) Feeding Trial Demonstrating the Enhanced Iron Bioavailability Properties of the Fast Cooking Manteca Yellow Bean (Phaseolus vulgaris L.). Nutrients, 11.","DOI":"10.3390\/nu11081768"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"649","DOI":"10.4141\/P04-160","article-title":"CDC Bronco field pea","volume":"85","author":"Warkentin","year":"2005","journal-title":"Can. J. Plant Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"74","DOI":"10.2135\/cropsci2011.05.0285","article-title":"Development and Characterization of Low-Phytate Pea","volume":"52","author":"Warkentin","year":"2012","journal-title":"Crop. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.4141\/cjps-2014-203","article-title":"CDC Limerick green field pea","volume":"94","author":"Warkentin","year":"2014","journal-title":"Can. J. Plant Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.4141\/cjps-2014-202","article-title":"CDC Raezer green field pea","volume":"94","author":"Warkentin","year":"2014","journal-title":"Can. J. Plant Sci."},{"key":"ref_44","unstructured":"AOAC (2012). Appendix J: AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces, AOAC Off. Methods Anal."},{"key":"ref_45","unstructured":"Jones, D.B. (1941). Factors for Converting Percentages of Nitrogen in Foods and Feeds into Percentages of Protein, Department of Agriculture-circ."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Tako, E., Bar, H., and Glahn, R.P. (2016). The Combined Application of the Caco-2 Cell Bioassay Coupled with In Vivo (Gallus gallus) Feeding Trial Represents an Effective Approach to Predicting Fe Bioavailability in Humans. Nutrients, 8.","DOI":"10.3390\/nu8110732"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1080\/03079450500267908","article-title":"Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahs","volume":"34","author":"Mete","year":"2005","journal-title":"Avian Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1042\/bj2580413","article-title":"Purification of chicken liver ferritin by two novel methods and structural comparison with horse spleen ferritin","volume":"258","author":"Passaniti","year":"1989","journal-title":"Biochem. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3134","DOI":"10.1021\/jf803647m","article-title":"Iron and Zinc Bioavailabilities to Pigs from Red and White Beans (Phaseolus vulgaris L.) Are Similar","volume":"57","author":"Tako","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.3382\/ps.2010-01080","article-title":"Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves, muscle growth, and body weight","volume":"90","author":"Kornasio","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0309-1740(87)90009-X","article-title":"Muscle glycogen: Composition of iodine binding and enzyme digestion assays and application to meat samples","volume":"20","author":"Dreiling","year":"1987","journal-title":"Meat Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1093\/ps\/84.5.764","article-title":"In ovo feeding improves energy status of late-term chicken embryos","volume":"84","author":"Uni","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jnutbio.2005.01.002","article-title":"Changes in chicken intestinal zinc exporter mRNA expression and small intestinal functionality following intra-amniotic zinc-methionine administration","volume":"16","author":"Tako","year":"2005","journal-title":"J. Nutr. Biochem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1093\/jn\/136.4.1064","article-title":"Symposium: Food Fortification in Developing Countries. Biofortification of Staple Food Crops","volume":"136","author":"Nestel","year":"2006","journal-title":"J. Nutr."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tako, E., Reed, S., Anandaraman, A., Beebe, S.E., Hart, J.J., and Glahn, R.P. (2015). Studies of Cream Seeded Carioca Beans (Phaseolus vulgaris L.) from a Rwandan Efficacy Trial: In Vitro and In Vivo Screening Tools Reflect Human Studies and Predict Beneficial Results from Iron Biofortified Beans. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0138479"},{"key":"ref_56","unstructured":"IBGE (2011). Instituto Brasileiro de Geografia e Estat\u00edstica, Coordena\u00e7\u00e3o de Trabalho e Rendimento, Pesquisa de Or\u00e7amentos Familiares: 2008\u20132009. An\u00e1lise Do Consumo Alimentar Pessoal No Brasil."},{"key":"ref_57","unstructured":"FAOSTAT (2019, April 04). Food and Agriculture Organization of the United Nations, Statistics Division. Forestry Production and Trade. Available online: http:\/\/www.fao.org\/faostat\/en\/#data\/FO."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1002\/jsfa.1742","article-title":"Effect of variety and crude protein content on nutrients and certain antinutrients in field peas (Pisum sativum)","volume":"84","author":"Wang","year":"2004","journal-title":"J. Sci. Food Agric."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/1745039X.2012.755326","article-title":"Nutritional evaluation of low-phytate peas (Pisum sativum L.) for young broiler chicks","volume":"67","author":"Thacker","year":"2013","journal-title":"Arch. Anim. Nutr."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1007\/s13197-013-0978-y","article-title":"Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains","volume":"52","author":"Gupta","year":"2013","journal-title":"J. Food Sci. Technol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1017\/S0960258500000039","article-title":"Phytic acid and phosphorus in crop seeds and fruits: A global estimate","volume":"10","author":"Lott","year":"2000","journal-title":"Seed Sci. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s001220050878","article-title":"Linkage mapping of two mutations that reduce phytic acid content of barley grain","volume":"97","author":"Larson","year":"1998","journal-title":"Theor. Appl. Genet."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1104\/pp.124.1.355","article-title":"Origin and Seed Phenotype of Maizelow phytic acid 1-1andlow phytic acid 2-1","volume":"124","author":"Raboy","year":"2000","journal-title":"Plant Physiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.2135\/cropsci2000.4061601x","article-title":"Isolation of High Seed Inorganic P, Low-Phytate Soybean Mutants","volume":"40","author":"Wilcox","year":"2000","journal-title":"Crop. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/j.1601-5223.1998.00107.x","article-title":"Identification of two Low-Phytate Barley (Hordeum Vulgare L.) Grain Mutants by TLC and Genetic Analysis","volume":"129","author":"Rasmussen","year":"2004","journal-title":"Hereditas"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.2135\/cropsci2000.4051397x","article-title":"Isolation and Genetic Mapping of a Non-Lethal Rice (Oryza sativa L.) low phytic acid 1 Mutation","volume":"40","author":"Larson","year":"2000","journal-title":"Crop. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1094\/CCHEM.2004.81.2.261","article-title":"Application of Wheat Meal Solvent Retention Capacity Tests Within Soft Wheat Breeding Populations","volume":"81","author":"Guttieri","year":"2004","journal-title":"Cereal Chem. J."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1439-0523.2008.01569.x","article-title":"Common bean (Phaseolus vulgaris L.) lines devoid of major lectin proteins","volume":"128","author":"Campion","year":"2009","journal-title":"Plant Breed."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1093\/ajcn\/56.3.573","article-title":"Soy protein, phytate, and iron absorption in humans","volume":"56","author":"Hurrell","year":"1992","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.lwt.2007.05.014","article-title":"Effect of pre-dehulling treatments on some nutritional and physical properties of navy and pinto beans (Phaseolus vulgaris L.)","volume":"41","author":"Anton","year":"2008","journal-title":"LWT"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.3945\/jn.113.175067","article-title":"Genetic Reduction of Phytate in Common Bean (Phaseolus vulgaris L.) Seeds Increases Iron Absorption in Young Women","volume":"143","author":"Petry","year":"2013","journal-title":"J. Nutr."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1475-2891-13-28","article-title":"Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgaris L.)","volume":"13","author":"Tako","year":"2014","journal-title":"Nutr. J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1475-2891-14-11","article-title":"Higher iron pearl millet (Pennisetum glaucum L.) provides more absorbable iron that is limited by increased polyphenolic content","volume":"14","author":"Tako","year":"2015","journal-title":"Nutr. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.3945\/jn.110.125369","article-title":"Polyphenols and Phytic Acid Contribute to the Low Iron Bioavailability from Common Beans in Young Women","volume":"140","author":"Petry","year":"2010","journal-title":"J. Nutr."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.3945\/jn.114.192989","article-title":"Phytic Acid Concentration Influences Iron Bioavailability from Biofortified Beans in Rwandese Women with Low Iron Status","volume":"144","author":"Petry","year":"2014","journal-title":"J. Nutr."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3285","DOI":"10.1021\/acs.jafc.6b05755","article-title":"Characterization of Polyphenol Effects on Inhibition and Promotion of Iron Uptake by Caco-2 Cells","volume":"65","author":"Hart","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s00394-015-0852-y","article-title":"Interaction of dietary compounds, especially polyphenols, with the intestinal microbiota: A review","volume":"54","author":"Tarko","year":"2015","journal-title":"Eur. J. Nutr."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/AEM.68.1.124-137.2002","article-title":"16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens","volume":"68","author":"Zhu","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.3382\/ps.2008-00091","article-title":"Factors Affecting Intestinal Health in Poultry","volume":"87","author":"Yegani","year":"2008","journal-title":"Poult. Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature08821","article-title":"A human gut microbial gene catalogue established by metagenomic sequencing","volume":"464","author":"Qin","year":"2010","journal-title":"Nature"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Adam, C.L., Williams, P.A., Garden, K.E., Thomson, L.M., and Ross, A.W. (2015). Dose-dependent effcts of soluble dietary fibre (pectin) on food intake, adiposity, guy hypertrophy and gut satiety hormone secretion in rats. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0115438"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Chen, X., Song, P., Fan, P., He, T., Jacobs, D., Levesque, C.L., Johnston, L.J., Ji, L., Ma, N., and Chen, Y. (2018). Moderate Dietary Protein Restriction Optimized Gut Microbiota and Mucosal Barrier in Growing Pig Model. Front. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00246"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/B978-0-12-800100-4.00003-9","article-title":"The role of short-chain fatty acids in health and disease","volume":"121","author":"Tan","year":"2014","journal-title":"Adv. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature11552","article-title":"Functional interactions between the gut microbiota and host metabolism","volume":"489","author":"Tremaroli","year":"2012","journal-title":"Nature"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"15718","DOI":"10.1073\/pnas.0407076101","article-title":"The gut microbiota as an environmental factor that regulates fat storage","volume":"101","author":"Backhed","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/9\/2563\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:05:48Z","timestamp":1760177148000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/9\/2563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,24]]},"references-count":85,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["nu12092563"],"URL":"https:\/\/doi.org\/10.3390\/nu12092563","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,24]]}}}