{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T21:24:42Z","timestamp":1785360282627,"version":"3.55.0"},"reference-count":58,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1C1B6002722"],"award-info":[{"award-number":["2018R1C1B6002722"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1D1A3B07040871"],"award-info":[{"award-number":["2018R1D1A3B07040871"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1I1A3A01060998"],"award-info":[{"award-number":["2019R1I1A3A01060998"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Cachexia induced by cancer is a systemic wasting syndrome and it accompanies continuous body weight loss with the exhaustion of skeletal muscle and adipose tissue. Cancer cachexia is not only a problem in itself, but it also reduces the effectiveness of treatments and deteriorates quality of life. However, effective treatments have not been found yet. Although Arctii Fructus (AF) has been studied about several pharmacological effects, there were no reports on its use in cancer cachexia. Methods: To induce cancer cachexia in mice, we inoculated CT-26 cells to BALB\/c mice through subcutaneous injection and intraperitoneal injection. To mimic cancer cachexia in vitro, we used conditioned media (CM), which was CT-26 colon cancer cells cultured medium. Results: In in vivo experiments, AF suppressed expression of interleukin (IL)-6 and atrophy of skeletal muscle and adipose tissue. As a result, the administration of AF decreased mortality by preventing weight loss. In adipose tissue, AF decreased expression of uncoupling protein 1 (UCP1) by restoring AMP-activated protein kinase (AMPK) activation. In in vitro model, CM increased muscle degradation factors and decreased adipocytes differentiation factors. However, these tendencies were ameliorated by AF treatment in C2C12 myoblasts and 3T3-L1 cells. Conclusion: Taken together, our study demonstrated that AF could be a therapeutic supplement for patients suffering from cancer cachexia.<\/jats:p>","DOI":"10.3390\/nu12103195","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T09:36:28Z","timestamp":1603100188000},"page":"3195","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Extract of Arctium lappa L. Fruit (Arctii Fructus) Improves Cancer-Induced Cachexia by Inhibiting Weight Loss of Skeletal Muscle and Adipose Tissue"],"prefix":"10.3390","volume":"12","author":[{"given":"Yo-Han","family":"Han","sequence":"first","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea"},{"name":"Department of Clinical and Administrative Pharmacy, College of Pharmacy, University of Georgia, Augusta, GA 30901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong-Geon","family":"Mun","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Dong","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae Hwan","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung-Min","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9836-2127","authenticated-orcid":false,"given":"Ji-Ye","family":"Kee","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Heon","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17105","DOI":"10.1038\/nrdp.2017.105","article-title":"Cancer-associated cachexia","volume":"4","author":"Baracos","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1079\/PNS19920036","article-title":"The Sir David Cuthbertson Medal Lecture 1991. The mechanisms and treatment of weight loss in cancer","volume":"51","author":"Fearon","year":"1992","journal-title":"Proc. Nutr. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s13539-012-0087-1","article-title":"Cancer cachexia: Impact, mechanisms and emerging treatments","volume":"4","author":"Vaughan","year":"2013","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.17987\/jcsm-cr.v3i2.65","article-title":"The Role of Systemic Inflammation in Cancer-Associated Muscle Wasting and Rationale for Exercise as a Therapeutic Intervention","volume":"3","author":"Cole","year":"2018","journal-title":"JCSM Clin. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.lungcan.2015.07.001","article-title":"Low muscle mass is associated with chemotherapy-induced haematological toxicity in advanced non-small cell lung cancer","volume":"90","author":"Sjoblom","year":"2015","journal-title":"Lung Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.lungcan.2019.05.021","article-title":"The prognostic value of early onset, CT derived loss of muscle and adipose tissue during chemotherapy in metastatic non-small cell lung cancer","volume":"133","author":"Degens","year":"2019","journal-title":"Lung Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1093\/annonc\/mdu086","article-title":"Validation of the Consensus-Definition for Cancer Cachexia and evaluation of a classification model\u2014A study based on data from an international multicentre project (EPCRC-CSA)","volume":"25","author":"Blum","year":"2014","journal-title":"Ann. Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1093\/gerona\/61.7.663","article-title":"Atrogin-1\/MAFbx and MuRF1 are downregulated in aging-related loss of skeletal muscle","volume":"61","author":"Edstrom","year":"2006","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.3892\/or.2015.3845","article-title":"Muscle-specific E3 ubiquitin ligases are involved in muscle atrophy of cancer cachexia: An in vitro and in vivo study","volume":"33","author":"Yuan","year":"2015","journal-title":"Oncol. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"E469","DOI":"10.1152\/ajpendo.00204.2014","article-title":"Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx\/atrogin-1","volume":"307","author":"Bodine","year":"2014","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1186\/s40064-016-2197-2","article-title":"Skeletal muscle wasting and renewal: A pivotal role of myokine IL-6","volume":"5","author":"Belizario","year":"2016","journal-title":"Springerplus"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.1016\/j.ejca.2015.07.042","article-title":"High subcutaneous adipose tissue predicts the prognosis in metastatic castration-resistant prostate cancer patients in post chemotherapy setting","volume":"51","author":"Antoun","year":"2015","journal-title":"Eur. J. Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.cmet.2014.06.011","article-title":"A switch from white to brown fat increases energy expenditure in cancer-associated cachexia","volume":"20","author":"Petruzzelli","year":"2014","journal-title":"Cell Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1101\/gad.1709008","article-title":"PPARgamma and C\/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale","volume":"22","author":"Lefterova","year":"2008","journal-title":"Genes. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5280","DOI":"10.3390\/nu6115280","article-title":"Evidence and mechanisms of fat depletion in cancer","volume":"6","author":"Ebadi","year":"2014","journal-title":"Nutrients"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1093\/annonc\/mdy114","article-title":"Systematic review and meta-analysis of the evidence for oral nutritional intervention on nutritional and clinical outcomes during chemo(radio)therapy: Current evidence and guidance for design of future trials","volume":"29","author":"Laviano","year":"2018","journal-title":"Ann. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.trecan.2016.07.005","article-title":"Cachexia & Brown Fat: A Burning Issue in Cancer","volume":"2","author":"Kir","year":"2016","journal-title":"Trends Cancer"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s12944-018-0657-0","article-title":"Interleukin-6 induces fat loss in cancer cachexia by promoting white adipose tissue lipolysis and browning","volume":"17","author":"Han","year":"2018","journal-title":"Lipids Health Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1142\/S0192415X17500720","article-title":"Arctii Fructus Inhibits Colorectal Cancer Cell Proliferation and MMPs Mediated Invasion via AMPK","volume":"45","author":"Han","year":"2017","journal-title":"Am. J. Chin. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5025","DOI":"10.1039\/C6FO01170E","article-title":"Anti-obesity effects of Arctii Fructus (Arctium lappa) in white\/brown adipocytes and high-fat diet-induced obese mice","volume":"7","author":"Han","year":"2016","journal-title":"Food Funct."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9443","DOI":"10.1021\/acs.jafc.7b02965","article-title":"Inhibition of Mast Cell-Mediated Allergic Responses by Arctii Fructus Extracts and Its Main Compound Arctigenin","volume":"65","author":"Kee","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Hall, D.T., Griss, T., Ma, J.F., Sanchez, B.J., Sadek, J., Tremblay, A.M.K., Mubaid, S., Omer, A., Ford, R.J., and Bedard, N. (2018). The AMPK agonist 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), but not metformin, prevents inflammation-associated cachectic muscle wasting. EMBO Mol. Med., 10.","DOI":"10.15252\/emmm.201708307"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"24214","DOI":"10.1038\/srep24214","article-title":"Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma","volume":"6","author":"Kim","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"152952","DOI":"10.1016\/j.phymed.2019.152952","article-title":"Rubrofusarin-6-beta-gentiobioside inhibits lipid accumulation and weight gain by regulating AMPK\/mTOR signaling","volume":"62","author":"Han","year":"2019","journal-title":"Phytomedicine"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"541","DOI":"10.4103\/0973-1296.141806","article-title":"Chemical analysis of raw and processed Fructus arctii by high-performance liquid chromatography\/diode array detection-electrospray ionization-mass spectrometry","volume":"10","author":"Qin","year":"2014","journal-title":"Pharmacogn. Mag."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1021\/jf010038a","article-title":"In vitro metabolism of plant lignans: New precursors of mammalian lignans enterolactone and enterodiol","volume":"49","author":"Heinonen","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1080\/10408360701612942","article-title":"Lignans and human health","volume":"44","author":"Adlercreutz","year":"2007","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1111\/j.1753-4887.2010.00319.x","article-title":"Dietary lignans: Physiology and potential for cardiovascular disease risk reduction","volume":"68","author":"Peterson","year":"2010","journal-title":"Nutr. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/aps.2018.32","article-title":"Overview of the anti-inflammatory effects, pharmacokinetic properties and clinical efficacies of arctigenin and arctiin from Arctium lappa L.","volume":"39","author":"Gao","year":"2018","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1476-9255-8-16","article-title":"Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-kappaB pathways","volume":"8","author":"Lee","year":"2011","journal-title":"J. Inflamm."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.jep.2009.01.038","article-title":"In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway","volume":"122","author":"Zhao","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Han, Y.H., Kee, J.Y., Kim, D.S., Mun, J.G., Jeong, M.Y., Park, S.H., Choi, B.M., Park, S.J., Kim, H.J., and Um, J.Y. (2016). Arctigenin Inhibits Lung Metastasis of Colorectal Cancer by Regulating Cell Viability and Metastatic Phenotypes. Molecules, 21.","DOI":"10.3390\/molecules21091135"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1007\/s005200050282","article-title":"Cachexia and anorexia: Cancer\u2019s covert killer","volume":"8","author":"Davis","year":"2000","journal-title":"Support. Care Cancer"},{"key":"ref_34","unstructured":"Rehman, I., and Rehman, A. (2020). Anatomy, Thorax, Heart, StatPearls."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1101\/gad.276733.115","article-title":"Mechanisms of metabolic dysfunction in cancer-associated cachexia","volume":"30","author":"Petruzzelli","year":"2016","journal-title":"Genes. Dev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1038\/nm.4171","article-title":"An AMP-activated protein kinase-stabilizing peptide ameliorates adipose tissue wasting in cancer cachexia in mice","volume":"22","author":"Rohm","year":"2016","journal-title":"Nat. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s13679-017-0283-6","article-title":"Brown Adipose Tissue: An Update on Recent Findings","volume":"6","author":"Marlatt","year":"2017","journal-title":"Curr. Obes. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1097\/MAJ.0000000000000511","article-title":"Mechanisms of Cachexia in Chronic Disease States","volume":"350","author":"Yoshida","year":"2015","journal-title":"Am. J. Med. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.3892\/ijmm.2015.2311","article-title":"Folic acid promotes the myogenic differentiation of C2C12 murine myoblasts through the Akt signaling pathway","volume":"36","author":"Hwang","year":"2015","journal-title":"Int. J. Mol. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1128\/CVI.00494-09","article-title":"Interactive changes between macrophages and adipocytes","volume":"17","author":"Xie","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"716","DOI":"10.7150\/jca.28246","article-title":"New Prospect for Cancer Cachexia: Medical Cannabinoid","volume":"10","author":"Wang","year":"2019","journal-title":"J. Cancer"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Advani, S.M., Advani, P.G., VonVille, H.M., and Jafri, S.H. (2018). Pharmacological management of cachexia in adult cancer patients: A systematic review of clinical trials. BMC Cancer, 18.","DOI":"10.1186\/s12885-018-5080-4"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1159\/000346446","article-title":"Clinical studies on the treatment of cancer cachexia with megestrol acetate plus thalidomide","volume":"58","author":"Wen","year":"2012","journal-title":"Chemotherapy"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4251\/wjgo.v7.i4.17","article-title":"Cancer cachexia, mechanism and treatment","volume":"7","author":"Aoyagi","year":"2015","journal-title":"World J. Gastrointest. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Yakabe, M., Ogawa, S., Ota, H., Iijima, K., Eto, M., Ouchi, Y., and Akishita, M. (2018). Inhibition of interleukin-6 decreases atrogene expression and ameliorates tail suspension-induced skeletal muscle atrophy. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191318"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s00424-008-0574-6","article-title":"Muscle wasting and interleukin-6-induced atrogin-I expression in the cachectic Apc (Min\/+) mouse","volume":"457","author":"Baltgalvis","year":"2009","journal-title":"Pflug. Arch."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"736563","DOI":"10.1155\/2014\/736563","article-title":"Effect of Sipjeondaebo-tang on cancer-induced anorexia and cachexia in CT-26 tumor-bearing mice","volume":"2014","author":"Choi","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.clnu.2009.11.006","article-title":"Loss of adipose tissue and plasma phospholipids: Relationship to survival in advanced cancer patients","volume":"29","author":"Murphy","year":"2010","journal-title":"Clin. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1152\/physrev.1998.78.3.783","article-title":"Understanding adipocyte differentiation","volume":"78","author":"Gregoire","year":"1998","journal-title":"Physiol. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/nm.3159","article-title":"PPARgamma signaling and metabolism: The good, the bad and the future","volume":"19","author":"Ahmadian","year":"2013","journal-title":"Nat. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1002\/jcb.25509","article-title":"Arctigenin Inhibits Adipogenesis by Inducing AMPK Activation and Reduces Weight Gain in High-Fat Diet-Induced Obese Mice","volume":"117","author":"Han","year":"2016","journal-title":"J. Cell Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.1194\/jlr.M600248-JLR200","article-title":"The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: Effects of obesity, insulin resistance, and TZD treatment","volume":"47","author":"Ranganathan","year":"2006","journal-title":"J. Lipid Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1194\/jlr.M039925","article-title":"Rohitukine inhibits in vitro adipogenesis arresting mitotic clonal expansion and improves dyslipidemia in vivo","volume":"55","author":"Varshney","year":"2014","journal-title":"J. Lipid Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1080\/09168451.2016.1217150","article-title":"Anti-adipogenic effect of Glycoside St-E2 and Glycoside St-C1 isolated from the leaves of Acanthopanax henryi (Oliv.) Harms in 3T3-L1 cells","volume":"80","author":"Han","year":"2016","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1142\/S0192415X16500312","article-title":"Lipin1-Mediated Repression of Adipogenesis by Rutin","volume":"44","author":"Han","year":"2016","journal-title":"Am. J. Chin. Med."},{"key":"ref_56","first-page":"842085","article-title":"Malnutrition and nutrition-therapy: Our neglected responsibility","volume":"2011","author":"Chermesh","year":"2011","journal-title":"Gastroenterol. Res. Pr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1177\/1535370216683282","article-title":"The role of adipose tissue in cancer-associated cachexia","volume":"242","author":"Vaitkus","year":"2017","journal-title":"Exp. Biol. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1002\/jmri.24053","article-title":"Comparison of brown and white adipose tissues in infants and children with chemical-shift-encoded water-fat MRI","volume":"38","author":"Hu","year":"2013","journal-title":"J. Magn. Reson. Imaging"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/10\/3195\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:23:58Z","timestamp":1760178238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/10\/3195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,19]]},"references-count":58,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["nu12103195"],"URL":"https:\/\/doi.org\/10.3390\/nu12103195","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,19]]}}}