{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T19:48:40Z","timestamp":1770061720302,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T00:00:00Z","timestamp":1603929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Royal Adelaide Hospital Endocrine Unit Gum Bequest Grant","award":["Royal Adelaide Hospital Endocrine Unit Gum Bequest Grant"],"award-info":[{"award-number":["Royal Adelaide Hospital Endocrine Unit Gum Bequest Grant"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Ageing is associated with changes in feeding behavior. We have reported that there is suppression of energy intake three hours after whey protein drink ingestion in young, but not older, men. This study aimed to determine these effects over a time period of 9 h. Fifteen younger (27 \u00b1 1 years, 25.8 \u00b1 0.7 kg\/m2) and 15 older (75 \u00b1 2 years, 26.6 \u00b1 0.8 kg\/m2) healthy men were studied on three occasions on which they received, in a randomized order, a 30 g\/120 kcal, 70 g\/280 kcal whey-protein, or control (~2 kcal) drink. Ad-libitum energy intake (sum of breakfast, lunch, and dinner) was suppressed in a protein load responsive fashion (P = 0.001). Suppression was minimal at breakfast, substantial at lunch (~\u221216%, P = 0.001), no longer present by dinner, and was less in older than younger men (\u22123 \u00b1 4% vs. \u22128 \u00b1 4%, P = 0.027). Cumulative protein intake was increased in the younger and older men (+20% and +42%, P &lt; 0.001). Visual analogue scale ratings of fullness were higher and desire to eat and prospective food consumption were lower after protein vs. control, and these effects were smaller in older vs. younger men (interaction effect P &lt; 0.05). These findings support the use of whey-protein drink supplements in older people who aim to increase their protein intake without decreasing their overall energy intake.<\/jats:p>","DOI":"10.3390\/nu12113318","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T09:44:53Z","timestamp":1603964693000},"page":"3318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Whey Protein Drink Ingestion before Breakfast Suppressed Energy Intake at Breakfast and Lunch, but Not during Dinner, and Was Less Suppressed in Healthy Older than Younger Men"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0517-8257","authenticated-orcid":false,"given":"Avneet","family":"Oberoi","sequence":"first","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5612-1918","authenticated-orcid":false,"given":"Caroline","family":"Giezenaar","sequence":"additional","affiliation":[{"name":"Riddet Institute, Massey University, Palmerston North 9430, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina","family":"Clames","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristine","family":"B\u00f8hler","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kylie","family":"Lange","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Horowitz","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1155-5816","authenticated-orcid":false,"given":"Karen L.","family":"Jones","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Chapman","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stijn","family":"Soenen","sequence":"additional","affiliation":[{"name":"Adelaide Medical School and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Royal Adelaide Hospital, Adelaide, SA 5000, South-Australia, Australia"},{"name":"Faculty of Health Sciences &amp; Medicine, Bond University, Gold Coast 4229, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"648","DOI":"10.3390\/healthcare3030648","article-title":"Aging, nutritional status and health","volume":"3","author":"Leslie","year":"2015","journal-title":"Healthcare"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.jamda.2013.02.004","article-title":"Body weight, anorexia, and undernutrition in older people","volume":"14","author":"Soenen","year":"2013","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Giezenaar, C., Chapman, I., Luscombe-Marsh, N., Feinle-Bisset, C., Horowitz, M., and Soenen, S. (2016). Ageing is associated with decreases in appetite and energy intake\u2014A meta-analysis in healthy adults. Nutrients, 8.","DOI":"10.3390\/nu8010028"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1177\/1941738113502296","article-title":"Muscle changes in aging: Understanding sarcopenia","volume":"6","author":"Siparsky","year":"2014","journal-title":"Sports Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.cger.2015.04.003","article-title":"Gastric emptying in the elderly","volume":"31","author":"Soenen","year":"2015","journal-title":"Clin. Geriatr. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1097\/MCO.0000000000000238","article-title":"The ageing gastrointestinal tract","volume":"19","author":"Soenen","year":"2016","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.arr.2011.12.008","article-title":"Systematic review and meta-analysis of the effects of high protein oral nutritional supplements","volume":"11","author":"Cawood","year":"2012","journal-title":"Ageing Res. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jamda.2012.08.001","article-title":"Effectiveness of nutritional supplementation on muscle mass in treatment of sarcopenia in old age: A systematic review","volume":"14","author":"Malafarina","year":"2013","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.3945\/ajcn.114.087981","article-title":"Effects of intraduodenal protein on appetite, energy intake, and antropyloroduodenal motility in healthy older compared with young men in a randomized trial","volume":"100","author":"Soenen","year":"2014","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"898.e9","DOI":"10.1016\/j.jamda.2017.06.027","article-title":"Effects of timing of whey protein intake on appetite and energy intake in healthy older men","volume":"18","author":"Giezenaar","year":"2017","journal-title":"J. Am. Med. Dir. Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"R845","DOI":"10.1152\/ajpregu.00213.2015","article-title":"Lesser suppression of energy intake by orally ingested whey protein in healthy older men compared with young controls","volume":"309","author":"Giezenaar","year":"2015","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_12","first-page":"136","article-title":"Therapeutic applications of whey protein","volume":"9","author":"Keri","year":"2004","journal-title":"Altern. Med. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.idairyj.2008.03.008","article-title":"Whey and whey proteins\u2014From \u2018gutter-to-gold\u2019","volume":"18","author":"Smithers","year":"2008","journal-title":"Int. Dairy J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1136\/gut.38.6.816","article-title":"Scintigraphic measurement of gastric emptying and ultrasonographic assessment of antral area: Relation to appetite","volume":"38","author":"Hveem","year":"1996","journal-title":"Gut"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/sj.ejcn.1601768","article-title":"Relation between food intake and visual analogue scale ratings of appetite and other sensations in healthy older and young subjects","volume":"58","author":"Parker","year":"2004","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1080\/003655200451153","article-title":"Effects of age on proximal gastric motor and sensory function","volume":"35","author":"Rayner","year":"2000","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Giezenaar, C., Lange, K., Hausken, T., Jones, K.L., Horowitz, M., Chapman, I., and Soenen, S. (2020). Effects of Age on Acute Appetite-Related Responses to Whey-Protein Drinks, Including Energy Intake, Gastric Emptying, Blood Glucose, and Plasma Gut Hormone Concentrations\u2014A Randomized Controlled Trial. Nutrients, 12.","DOI":"10.3390\/nu12041008"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"865","DOI":"10.3945\/ajcn.117.154377","article-title":"Effects of randomized whey-protein loads on energy intake, appetite, gastric emptying, and plasma gut-hormone concentrations in older men and women","volume":"106","author":"Giezenaar","year":"2017","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Giezenaar, C., Van Der Burgh, Y., Lange, K., Hatzinikolas, S., Hausken, T., Jones, K.L., Horowitz, M., Chapman, I., and Soenen, S. (2018). Effects of Substitution, and Adding of Carbohydrate and Fat to Whey-Protein on Energy Intake, Appetite, Gastric Emptying, Glucose, Insulin, Ghrelin, CCK and GLP-1 in Healthy Older Men\u2014A Randomized Controlled Trial. Nutrients, 10.","DOI":"10.3390\/nu10020113"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.3945\/ajcn.115.117556","article-title":"Acute load-dependent effects of oral whey protein on gastric emptying, gut hormone release, glycemia, appetite, and energy intake in healthy men","volume":"102","author":"Hutchison","year":"2015","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1093\/ajcn\/80.3.656","article-title":"Energy intake and appetite are related to antral area in healthy young and older subjects","volume":"80","author":"Sturm","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Giezenaar, C., Hutchison, A.T., Luscombe-Marsh, N.D., Chapman, I., Horowitz, M., and Soenen, S. (2017). Effect of age on blood glucose and plasma insulin, glucagon, ghrelin, CCK, GIP, and GLP-1 responses to whey protein ingestion. Nutrients, 10.","DOI":"10.3390\/nu10010002"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1111\/j.1365-2036.2011.04609.x","article-title":"The role of gastric motility in the control of food intake","volume":"33","author":"Janssen","year":"2011","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/ajcn\/66.1.127","article-title":"Relation between postprandial satiation and antral area in normal subjects","volume":"66","author":"Jones","year":"1997","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"E Rigamonti, A., Leoncini, R., Casnici, C., Marelli, O., De Col, A., Tamini, S., Lucchetti, E., Cicolini, S., Abbruzzese, L., and Cella, S. (2019). Whey Proteins Reduce Appetite, Stimulate Anorexigenic Gastrointestinal Peptides and Improve Glucometabolic Homeostasis in Young Obese Women. Nutrients, 11.","DOI":"10.3390\/nu11020247"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1097\/MCO.0000000000000011","article-title":"Dietary protein and muscle in older persons","volume":"17","author":"Paddon","year":"2014","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/ajcn\/62.5.923","article-title":"Age-related impairments in the regulation of food intake","volume":"62","author":"Rolls","year":"1995","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/s41387-018-0048-7","article-title":"Effect of gender on the acute effects of whey protein ingestion on energy intake, appetite, gastric emptying and gut hormone responses in healthy young adults","volume":"8","author":"Giezenaar","year":"2018","journal-title":"Nutr. Diabetes"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"B174","DOI":"10.1093\/geronj\/43.6.B174","article-title":"Calorie and nutrient intakes of elderly and young subjects measured under identical conditions","volume":"43","author":"Wurtman","year":"1988","journal-title":"J. Gerontol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"R243","DOI":"10.1152\/ajpregu.1997.272.1.R243","article-title":"Evidence for the anorexia of aging: Gastrointestinal transit and hunger in healthy elderly vs. young adults","volume":"272","author":"Clarkston","year":"1997","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3747","DOI":"10.1210\/jc.2002-021656","article-title":"Appetite, food intake, and plasma concentrations of cholecystokinin, ghrelin, and other gastrointestinal hormones in undernourished older women and well-nourished young and older women","volume":"88","author":"Sturm","year":"2003","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1001\/jama.1994.03520200057036","article-title":"Control of food intake in older men","volume":"272","author":"Roberts","year":"1994","journal-title":"JAMA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1111\/dom.12056","article-title":"Higher protein diet for weight management in young overweight women: A 12-month randomized controlled trial","volume":"15","author":"Griffin","year":"2013","journal-title":"Diabetes. Obes. Metab."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/oby.2007.531","article-title":"Higher protein intake preserves lean mass and satiety with weight loss in pre-obese and obese women","volume":"15","author":"Leidy","year":"2007","journal-title":"Obesity"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/11\/3318\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:26:19Z","timestamp":1760178379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/11\/3318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,29]]},"references-count":34,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["nu12113318"],"URL":"https:\/\/doi.org\/10.3390\/nu12113318","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,29]]}}}