{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:03:28Z","timestamp":1785521008452,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T00:00:00Z","timestamp":1604016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004488","name":"Croatian Science Foundation","doi-asserted-by":"publisher","award":["IP-2016-06-3163"],"award-info":[{"award-number":["IP-2016-06-3163"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The objective of this study was to evaluate the influence of high-fat (HF) and cafeteria diet (CAF) diets and sex on the metabolism of important fatty acids in the liver and perirenal fat tissue. Dietary treatments induced changes in the fatty acid profile in comparison to the untreated group, but the characteristic differences between treated groups were also observable. The HF diet induced an increase in the content of C16:1n-7 and C18:1n-7 in the liver phospholipids (PL) and triglycerides (TG) and perirenal fat tissue compared to the control and CAF diet. The CAF diet induced a more drastic decrease in both n-3 and n-6 polyunsaturated fatty acids (PUFA), including depletion of eicosapentaenoic acid (EPA). The CAF diet also increased the content of n-6 docosapentaenoic acid (DPAn-6) in the liver and decreased it in the perirenal fat. Sex also had a significant influence on the fatty acid profile, but the variables with the highest differences between the CAF and HF treatments were identical in the male and female rats. In this study, we have established that two dietary models of non-alcoholic fatty liver disease (NAFLD) led to characteristic changes in the hepatic and perirenal fat fatty acid profile, in contrast to the control diet and in comparison with each other. These differences could play an important role in the interpretation of the experimental results of nutritional studies.<\/jats:p>","DOI":"10.3390\/nu12113339","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T09:29:32Z","timestamp":1604050172000},"page":"3339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Cafeteria Diet and High-Fructose Rodent Models of NAFLD Differ in the Metabolism of Important PUFA and Palmitoleic Acid without Additional Influence of Sex"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6103-4728","authenticated-orcid":false,"given":"Tomislav","family":"Ma\u0161ek","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josip","family":"Bari\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Biotechnology in Aquaculture, Division of Materials Chemistry, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka cesta 54, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vedran","family":"Micek","sequence":"additional","affiliation":[{"name":"Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2606-7807","authenticated-orcid":false,"given":"Kristina","family":"Star\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.4254\/wjh.v9.i16.715","article-title":"Non-alcoholic fatty liver disease: An expanded review","volume":"9","author":"Benedict","year":"2017","journal-title":"World J. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.cld.2015.10.001","article-title":"Epidemiology of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis in the United States and the Rest of the World","volume":"20","author":"Sayiner","year":"2016","journal-title":"Clin. Liver Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bertot, L.C., and Adams, L.A. (2016). The Natural Course of Non-Alcoholic Fatty Liver Disease. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17050774"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Van Herck, M.A., Vonghia, L., and Francque, S.M. (2017). Animal Models of Nonalcoholic Fatty Liver Disease-A Starter\u2019s Guide. Nutrients, 9.","DOI":"10.3390\/nu9101072"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1038\/oby.2011.18","article-title":"Cafeteria Diet Is a Robust Model of Human Metabolic Syndrome with Liver and Adipose Inflammation: Comparison to High-Fat Diet","volume":"19","author":"Sampey","year":"2011","journal-title":"Obesity"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1194\/jlr.M067801","article-title":"Differential effects of EPA versus DHA on postprandial vascular function and the plasma oxylipin profile in men","volume":"57","author":"McManus","year":"2016","journal-title":"J. Lipid Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1210\/jc.2017-02745","article-title":"High-Dose DHA Has More Profound Effects on LDL-Related Features Than High-Dose EPA: The ComparED Study","volume":"103","author":"Allaire","year":"2018","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Urli\u0107, M., Urli\u0107, I., Urli\u0107, H., Ma\u0161ek, T., Benzon, B., Vitlov Uljevi\u0107, M., Vukojevi\u0107, K., and Filipovi\u0107, N. (2020). Effects of Different n6\/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy. Nutrients, 12.","DOI":"10.3390\/nu12092761"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"173S","DOI":"10.3945\/an.115.011130","article-title":"The Role of the Novel Lipokine Palmitoleic Acid in Health and Disease","volume":"8","author":"Frigolet","year":"2017","journal-title":"Adv. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Star\u010devi\u0107, K., Filipovi\u0107, N., Galan, A., Micek, V., Kurilj, A.G., and Ma\u0161ek, T. (2018). Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6\/n3 Ratios and DHA\/EPA Supplementation in Streptozotocin Treated Rats. Mol. Nutr. Food Res., 62.","DOI":"10.1002\/mnfr.201701007"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.cccn.2004.10.013","article-title":"Evaluation of Roche Accu-Chek Go and Medisense Optium blood glucose meters","volume":"353","author":"Hawkins","year":"2005","journal-title":"Clin. Chim. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/BF00280883","article-title":"Homeostasis model assessment: Insulin resistance and \u03b2-cell function from fasting plasma glucose and insulin concentrations in man","volume":"28","author":"Matthews","year":"1985","journal-title":"Diabetologia"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1210\/jcem.85.7.6661","article-title":"Quantitative Insulin Sensitivity Check Index: A Simple, Accurate Method for Assessing Insulin Sensitivity In Humans","volume":"85","author":"Katz","year":"2000","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Klopfleisch, R. (2013). Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology-a systematic review. BMC Veter Res., 9.","DOI":"10.1186\/1746-6148-9-123"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u2206\u2206 CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_17","first-page":"e54360","article-title":"Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils","volume":"10","author":"Quideau","year":"2016","journal-title":"J. Vis. Exp."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Jouihan, H. (2012). Measurement of Liver Triglyceride Content. Bio-Protocol, 2.","DOI":"10.21769\/BioProtoc.223"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"G13","DOI":"10.1530\/JOE-14-0182","article-title":"METABOLIC PHENOTYPING GUIDELINES: Assessing glucose homeostasis in rodent models","volume":"222","author":"Bowe","year":"2014","journal-title":"J. Endocrinol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plefa.2016.11.001","article-title":"Effects of treatment with sucrose in drinking water on liver histology, lipogenesis and lipogenic gene expression in rats fed high-fiber diet","volume":"116","author":"Vuica","year":"2017","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13098-018-0307-8","article-title":"Dietary fat stimulates development of NAFLD more potently than dietary fructose in Sprague\u2013Dawley rats","volume":"10","author":"Jensen","year":"2018","journal-title":"Diabetol. Metab. Syndr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1002\/hep.30626","article-title":"Sex Differences in Nonalcoholic Fatty Liver Disease: State of the Art and Identification of Research Gaps","volume":"70","author":"Lonardo","year":"2019","journal-title":"Hepatology"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Da Silva-Santi, L.G., Antunes, M.M., Caparroz-Assef, S.M., Carbonera, F., Masi, L.N., Curi, R., Visentainer, J.V., and Bazotte, R.B. (2016). Liver Fatty Acid Composition and Inflammation in Mice Fed with High-Carbohydrate Diet or High-Fat Diet. Nutrients, 8.","DOI":"10.3390\/nu8110682"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.biochi.2004.10.002","article-title":"Effects of streptozotocin and dietary fructose on delta-6 desaturation in spontaneously hypertensive rat liver","volume":"86","author":"Comte","year":"2004","journal-title":"Biochimie"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12263-017-0551-9","article-title":"Effect of high carbohydrate diet on elongase and desaturase activity and accompanying gene expression in rat\u2019s liver","volume":"12","author":"Gawedzka","year":"2017","journal-title":"Genes Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1248\/bpb.b16-00470","article-title":"Fatty Acid \u03b2-Oxidation Plays a Key Role in Regulating cis-Palmitoleic Acid Levels in the Liver","volume":"39","author":"Kawabata","year":"2016","journal-title":"Biol. Pharm. Bull."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1111\/jgh.14654","article-title":"Serum C16:1n7\/C16:0 ratio as a diagnostic marker for non-alcoholic steatohepatitis Diagnosis of NASH using serum fatty acid","volume":"34","author":"Yamada","year":"2019","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.plefa.2005.06.004","article-title":"Effects of fenofibrate and insulin on the biosynthesis of unsaturated fatty acids in streptozotocin diabetic rats","volume":"73","author":"Montanaro","year":"2005","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s11745-003-1121-x","article-title":"Desaturase activities in rat model of insulin resistance induced by a sucrose-rich diet","volume":"38","author":"Brenner","year":"2003","journal-title":"Lipids"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/S0955-2863(01)00154-1","article-title":"Effect of sucrose addition to drinking water, that induces hypertension in the rats, on liver microsomal \u22069 and \u03945-desaturase activities","volume":"12","year":"2001","journal-title":"J. Nutr. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00380-008-1098-x","article-title":"Castration modifies aortic vasoreactivity and serum fatty acids in a sucrose-fed rat model of metabolic syndrome","volume":"24","author":"Carvajal","year":"2009","journal-title":"Hear. Vessel."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1080\/10408398.2018.1425978","article-title":"How does high DHA fish oil affect health? A systematic review of evidence","volume":"59","author":"Fard","year":"2018","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"248","DOI":"10.5551\/jat.19976","article-title":"An Increase in the EPA\/AA Ratio is Associated with Improved Arterial Stiffness in Obese Patients with Dyslipidemia","volume":"21","author":"Ito","year":"2014","journal-title":"J. Atheroscler. Thromb."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.atherosclerosis.2016.04.026","article-title":"Eicosapentaenoic acid to arachidonic acid (EPA\/AA) ratio as an associated factor of high risk plaque on coronary computed tomography in patients without coronary artery disease","volume":"250","author":"Nagahara","year":"2016","journal-title":"Atherosclerosis"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12974-016-0714-4","article-title":"Reduced acute neuroinflammation and improved functional recovery after traumatic brain injury by \u03b1-linolenic acid supplementation in mice","volume":"13","author":"Desai","year":"2016","journal-title":"J. Neuroinflamm."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/11\/3339\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:26:54Z","timestamp":1760178414000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/12\/11\/3339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,30]]},"references-count":35,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["nu12113339"],"URL":"https:\/\/doi.org\/10.3390\/nu12113339","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,30]]}}}