{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T20:32:38Z","timestamp":1781641958319,"version":"3.54.5"},"reference-count":35,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T00:00:00Z","timestamp":1608249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002284","name":"Asthma Australia","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100002284","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001771","name":"University of Newcastle Australia","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Measurement of vitamin D status has significant use in clinical and research settings, including during pregnancy. We aimed to assess the agreement of total 25-hydroxyvitamin D (25(OH)D) concentration, and its three analytes (25-hydroxyvitamin D3 (25(OH)D3), 25-hydroxyvitamin D2 (25(OH)D2) and Epi-25-hydroxyvitamin D3 (Epi-25(OH)D3)), in plasma and serum samples collected during pregnancy, and to examine the proportion of women who change vitamin D status category based on sample type. Matching samples were collected from n = 114 non-fasting women between 12\u201325 weeks gestation in a clinical trial in Newcastle, Australia. Samples were analysed by liquid chromatography-tandem mass-spectrometry (LC-MS\/MS) to quantify total 25(OH)D and its analytes and examined using Bland-Altman plots, Pearson correlation (r), intraclass correlation coefficient and Cohen\u2019s Kappa test. Serum total 25(OH)D ranged from 33.8\u2013169.8 nmol\/L and plasma ranged from 28.6\u2013211.2 nmol\/L. There was a significant difference for total 25(OH)D based on sample type (measurement bias 7.63 nmol\/L for serum vs plasma (95% Confidence Interval (CI) 5.36, 9.90, p \u2264 0.001). The mean difference between serum and plasma concentrations was statistically significant for 25(OH)D3 (7.38 nmol\/L; 95% CI 5.28, 9.48, p \u2264 0.001) and Epi-25(OH)D3 (0.39 nmol\/L; 95% CI 0.14, 0.64, p = 0.014). Of 114 participants, 28% were classified as vitamin D deficient (&lt;50 nmol\/L) or insufficient (&lt;75 nmol\/L) based on plasma sample and 36% based on serum sample. Nineteen (16.7%) participants changed vitamin D status category based on sample type. 25-hydroxyvitamin D quantification using LC-MS\/MS methodology differed significantly between serum and plasma, yielding a higher value in plasma; this influenced vitamin D status based on accepted cut-points, which may have implications in clinical and research settings.<\/jats:p>","DOI":"10.3390\/nu12123872","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T11:42:09Z","timestamp":1608291729000},"page":"3872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Impact of Sample Type on Vitamin D Quantification and Clinical Classification during Pregnancy"],"prefix":"10.3390","volume":"12","author":[{"given":"Soriah M.","family":"Harvey","sequence":"first","affiliation":[{"name":"Priority Research Centre Grow Up Well, Hunter Medical Research Institute, School of Medicine and Public Health, University of Newcastle, Callaghan, NSW 2305, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa E.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Priority Research Centre Grow Up Well, Hunter Medical Research Institute, School of Medicine and Public Health, University of Newcastle, Callaghan, NSW 2305, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter G.","family":"Gibson","sequence":"additional","affiliation":[{"name":"Priority Research Centre for Healthy Lungs, University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW 2305, Australia"},{"name":"Department of Respiratory and Sleep Medicine, John Hunter Hospital, New Lambton Heights, NSW 2305, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Clarke","sequence":"additional","affiliation":[{"name":"Metabolomics Australia, Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA 6000, Australia"},{"name":"School of Molecular Sciences, The University of Western Australia, Perth, WA 6000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8653-4801","authenticated-orcid":false,"given":"Megan E.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Priority Research Centre Grow Up Well, Hunter Medical Research Institute, School of Medicine and Public Health, University of Newcastle, Callaghan, NSW 2305, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,18]]},"reference":[{"key":"ref_1","unstructured":"National Institutes of Health (2020, October 01). 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