{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:57:48Z","timestamp":1789495068917,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Lupine flour is a valuable food due to its favorable nutritional properties. In spite of its allergenic potential, its use is increasing. Three lupine species, Lupinus angustifolius, L. luteus, and L. albus are relevant for human nutrition. The aim of this study is to clarify whether the species differ with regard to their allergen composition and whether anaphylaxis marker allergens could be identified in lupine. Patients with the following characteristics were included: lupine allergy, suspected lupine allergy, lupine sensitization only, and peanut allergy. Lupine sensitization was detected via CAP-FEIA (ImmunoCAP) and skin prick test. Protein, DNA and expressed sequence tag (EST) databases were queried for lupine proteins homologous to already known legume allergens. Different extraction methods applied on seeds from all species were examined by SDS-PAGE and screened by immunoblotting for IgE-binding proteins. The extracts underwent different and successive chromatography methods. Low-molecular-weight components were purified and investigated for IgE-reactivity. Proteomics revealed a molecular diversity of the three species, which was confirmed when investigated for IgE-reactivity. Three new allergens, L. albus profilin, L. angustifolius and L. luteus lipid transfer protein (LTP), were identified. LTP as a potential marker allergen for severity is a valuable additional candidate for molecular allergy diagnostic tests.<\/jats:p>","DOI":"10.3390\/nu13020409","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T05:23:05Z","timestamp":1611811385000},"page":"409","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Identification and Purification of Novel Low-Molecular-Weight Lupine Allergens as Components for Personalized Diagnostics"],"prefix":"10.3390","volume":"13","author":[{"given":"Uta","family":"Jappe","sequence":"first","affiliation":[{"name":"Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany"},{"name":"Interdisciplinary Allergy Outpatient Clinic, Department of Internal Medicine and Pneumology, University of L\u00fcbeck, 23538 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arabella","family":"Karstedt","sequence":"additional","affiliation":[{"name":"Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Warneke","sequence":"additional","affiliation":[{"name":"Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Hellmig","sequence":"additional","affiliation":[{"name":"Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"B\u00f6ttger","sequence":"additional","affiliation":[{"name":"Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich W.","family":"Riffelmann","sequence":"additional","affiliation":[{"name":"Department of Allergology, Fachkrankenhaus Kloster Grafschaft, 57392 Schmallenberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9642-4742","authenticated-orcid":false,"given":"Regina","family":"Treudler","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venerology and Allergology, Universit\u00e4t Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Lange","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, St. Marien-Hospital, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7457-6481","authenticated-orcid":false,"given":"Susanne","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Allergy Center, Medical Faculty Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3339-0709","authenticated-orcid":false,"given":"Sabine","family":"D\u00f6lle-Bierke","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology, Department of Dermatology, Venerology and Allergology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3449-1245","authenticated-orcid":false,"given":"Margitta","family":"Worm","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology, Department of Dermatology, Venerology and Allergology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Ru\u00ebff","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology, Klinikum der Universit\u00e4t M\u00fcnchen, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Reese","sequence":"additional","affiliation":[{"name":"Allergopharma, 21465 Reinbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 C.","family":"Knulst","sequence":"additional","affiliation":[{"name":"Department of Dermatology\/Allergology, University Medical Center Utrecht, Utrecht University, 3508 GA Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolf-Meinhard","family":"Becker","sequence":"additional","affiliation":[{"name":"Division of Clinical and Molecular Allergology, Research Center Borstel, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/j.1398-9995.2008.01818.x","article-title":"Clinical relevance of sensitization to lupine in peanut-sensitized adults","volume":"64","author":"Peeters","year":"2009","journal-title":"Allergy"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1093\/ajcn\/84.5.975","article-title":"Lupin-enriched bread increases satiety and reduces energy intake acutely","volume":"84","author":"Lee","year":"2006","journal-title":"Am. 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