{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T02:07:01Z","timestamp":1773972421165,"version":"3.50.1"},"reference-count":67,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:00:00Z","timestamp":1611964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Vitamin E (VitE) is essential for vertebrate embryogenesis, but the mechanisms involved remain unknown. To study embryonic development, we fed zebrafish adults (&gt;55 days) either VitE sufficient (E+) or deficient (E\u2013) diets for &gt;80 days, then the fish were spawned to generate E+ and E\u2013 embryos. To evaluate the transcriptional basis of the metabolic and phenotypic outcomes, E+ and E\u2013 embryos at 12, 18 and 24 h post-fertilization (hpf) were subjected to gene expression profiling by RNASeq. Hierarchical clustering, over-representation analyses and gene set enrichment analyses were performed with differentially expressed genes. E\u2013 embryos experienced overall disruption to gene expression associated with gene transcription, carbohydrate and energy metabolism, intracellular signaling and the formation of embryonic structures. mTOR was apparently a major controller of these changes. Thus, embryonic VitE deficiency results in genetic and transcriptional dysregulation as early as 12 hpf, leading to metabolic dysfunction and ultimately lethal outcomes.<\/jats:p>","DOI":"10.3390\/nu13020468","type":"journal-article","created":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T08:56:01Z","timestamp":1611996961000},"page":"468","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Vitamin E Deficiency Disrupts Gene Expression Networks during Zebrafish Development"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-2227","authenticated-orcid":false,"given":"Brian","family":"Head","sequence":"first","affiliation":[{"name":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA"},{"name":"Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR 97331, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Ramsey","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA"},{"name":"School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, OR 97331, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8226-9908","authenticated-orcid":false,"given":"Chrissa","family":"Kioussi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6190-3682","authenticated-orcid":false,"given":"Robyn L.","family":"Tanguay","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2892-4024","authenticated-orcid":false,"given":"Maret G.","family":"Traber","sequence":"additional","affiliation":[{"name":"Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA"},{"name":"School of Biological and Population Health Sciences, College of Public Health, Oregon State University, Corvallis, OR 97331, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.freeradbiomed.2007.03.024","article-title":"Vitamin E, antioxidant and nothing more","volume":"43","author":"Traber","year":"2007","journal-title":"Free Radic. Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/iub.1986","article-title":"Vitamin E: Regulatory Role on Signal Transduction","volume":"71","author":"Zingg","year":"2019","journal-title":"IUBMB Life"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.plaphy.2009.11.004","article-title":"Biosynthesis, regulation and functions of tocochromanols in plants","volume":"48","author":"DellaPenna","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1126\/science.56.1458.650","article-title":"On the Existence of a Hitherto Unrecognized Dietary Factor Essential for Reproduction","volume":"56","author":"Evans","year":"1922","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1530\/jrf.0.0380115","article-title":"The effect of vitamin E and synthetic antioxidants on the growth in vitro of explanted rat embryos","volume":"38","author":"Steele","year":"1974","journal-title":"J. Reprod. Fertil."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1095\/biolreprod.105.043018","article-title":"Vitamin E is essential for mouse placentation but not for embryonic development itself","volume":"73","author":"Jishage","year":"2005","journal-title":"Biol. Reprod."},{"key":"ref_7","unstructured":"Altman, P.L., and Dittmer, D.S. (1962). Growth, including reproduction and morphological development, Federation of American Societies for Experimental Biology."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/aja.1002030302","article-title":"Stages of embryonic development of the zebrafish","volume":"203","author":"Kimmel","year":"1995","journal-title":"Dev. Dyn."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Miller, G.W., Ulatowski, L., Labut, E.M., Lebold, K.M., Manor, D., Atkinson, J., Barton, C.L., Tanguay, R.L., and Traber, M.G. (2012). The alpha-tocopherol transfer protein is essential for vertebrate embryogenesis. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047402"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.jnutbio.2011.02.002","article-title":"Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality","volume":"23","author":"Miller","year":"2012","journal-title":"J. Nutr. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.freeradbiomed.2017.01.020","article-title":"Lethal dysregulation of energy metabolism during embryonic vitamin E deficiency","volume":"104","author":"McDougall","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"15028","DOI":"10.1038\/s41598-020-71760-x","article-title":"Vitamin E is necessary for zebrafish nervous system development","volume":"10","author":"Head","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Santander, N., Lizama, C., Parga, M.J., Quiroz, A., Perez, D., Echeverria, G., Ulloa, L., Palma, V., Rigotti, A., and Busso, D. (2017). Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI. Sci. Rep.-Uk, 7.","DOI":"10.1038\/s41598-017-05422-w"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/tera.1420510102","article-title":"Exencephaly and hydrocephaly in mice with targeted modification of the apolipoprotein B (Apob) gene","volume":"51","author":"Homanics","year":"1995","journal-title":"Teratology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.neuroscience.2013.12.001","article-title":"Vitamin E is essential for Purkinje neuron integrity","volume":"260","author":"Ulatowski","year":"2014","journal-title":"Neuroscience"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"15185","DOI":"10.1073\/pnas.261456098","article-title":"Delayed-onset ataxia in mice lacking alpha -tocopherol transfer protein: Model for neuronal degeneration caused by chronic oxidative stress","volume":"98","author":"Yokota","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"18982","DOI":"10.1073\/pnas.1301262110","article-title":"Glycolysis supports embryonic muscle growth by promoting myoblast fusion","volume":"110","author":"Tixier","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.dib.2017.02.046","article-title":"Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization","volume":"11","author":"McDougall","year":"2017","journal-title":"Data Brief."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101784","DOI":"10.1016\/j.redox.2020.101784","article-title":"Vitamin E deficiency dysregulates thiols, amino acids and related molecules during zebrafish embryogenesis","volume":"38","author":"Zhang","year":"2020","journal-title":"Redox. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lee, M.S., Bonner, J.R., Bernard, D.J., Sanchez, E.L., Sause, E.T., Prentice, R.R., Burgess, S.M., and Brody, L.C. (2012). Disruption of the folate pathway in zebrafish causes developmental defects. BMC Dev. Biol., 12.","DOI":"10.1186\/1471-213X-12-12"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"White, R.J., Collins, J.E., Sealy, I.M., Wali, N., Dooley, C.M., Digby, Z., Stemple, D.L., Murphy, D.N., Billis, K., and Hourlier, T. (2017). A high-resolution mRNA expression time course of embryonic development in zebrafish. Elife, 6.","DOI":"10.7554\/eLife.30860"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/cr.2015.146","article-title":"Multiple amino acid sensing inputs to mTORC1","volume":"26","author":"Shimobayashi","year":"2016","journal-title":"Cell Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.neuint.2018.10.007","article-title":"Systemic L-buthionine-S-R-sulfoximine administration modulates glutathione homeostasis via NGF\/TrkA and mTOR signaling in the cerebellum","volume":"121","author":"Petrosyan","year":"2018","journal-title":"Neurochem. Int."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"14861","DOI":"10.1523\/JNEUROSCI.4968-14.2015","article-title":"Fbxw7 Limits Myelination by Inhibiting mTOR Signaling","volume":"35","author":"Kearns","year":"2015","journal-title":"J. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4887","DOI":"10.1038\/s41467-019-12640-5","article-title":"DNA damage and transcription stress cause ATP-mediated redesign of metabolism and potentiation of anti-oxidant buffering","volume":"10","author":"Milanese","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0022-2275(20)37587-8","article-title":"Simultaneous determination of tissue tocopherols, tocotrienols, ubiquinols, and ubiquinones","volume":"37","author":"Podda","year":"1996","journal-title":"J. Lipid Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"W199","DOI":"10.1093\/nar\/gkz401","article-title":"WebGestalt 2019: Gene set analysis toolkit with revamped UIs and APIs","volume":"47","author":"Liao","year":"2019","journal-title":"Nucleic. Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D867","DOI":"10.1093\/nar\/gky1090","article-title":"The Zebrafish Information Network: New support for non-coding genes, richer Gene Ontology annotations and the Alliance of Genome Resources","volume":"47","author":"Ruzicka","year":"2019","journal-title":"Nucleic. Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Pang, Z., Chong, J., Li, S., and Xia, J. (2020). MetaboAnalystR 3.0: Toward an Optimized Workflow for Global Metabolomics. Metabolites, 10.","DOI":"10.3390\/metabo10050186"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.nbd.2017.09.004","article-title":"mTOR-related neuropathology in mutant tsc2 zebrafish: Phenotypic, transcriptomic and pharmacological analysis","volume":"108","author":"Scheldeman","year":"2017","journal-title":"Neurobiol. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1002\/dvdy.23838","article-title":"Expression and retinoic acid regulation of the zebrafish nr2f orphan nuclear receptor genes","volume":"241","author":"Love","year":"2012","journal-title":"Dev. Dyn."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nprot.2007.514","article-title":"High-resolution in situ hybridization to whole-mount zebrafish embryos","volume":"3","author":"Thisse","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3181\/00379727-135-34985","article-title":"Glucose metabolism by rat embryos in vitro","volume":"135","author":"Tanimura","year":"1970","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.cbd.2014.02.001","article-title":"The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome","volume":"10","author":"Miller","year":"2014","journal-title":"Comp. Biochem. Physiol. Part D Genomics. Proteomics."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1021\/pr401108d","article-title":"Proteome-driven elucidation of adaptive responses to combined vitamin E and C deficiency in zebrafish","volume":"13","author":"Motorykin","year":"2014","journal-title":"J. Proteome. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1002\/nbm.1615","article-title":"Changes in the metabolic profile of rat liver after alpha-tocopherol deficiency as revealed by metabolomics analysis","volume":"24","author":"Moazzami","year":"2011","journal-title":"NMR Biomed."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.3945\/jn.111.144279","article-title":"Vitamin E deficiency decreases long-chain PUFA in zebrafish (Danio rerio)","volume":"141","author":"Lebold","year":"2011","journal-title":"J. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.cbpc.2013.03.007","article-title":"Chronic vitamin E deficiency promotes vitamin C deficiency in zebrafish leading to degenerative myopathy and impaired swimming behavior","volume":"157","author":"Lebold","year":"2013","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1016\/j.celrep.2017.10.098","article-title":"Biological Significance of the Suppression of Oxidative Phosphorylation in Induced Pluripotent Stem Cells","volume":"21","author":"Zhang","year":"2017","journal-title":"Cell Rep"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Kraus, P., V, S., Yu, H.B., Xing, X., Lim, S.L., Adler, T., Pimentel, J.A., Becker, L., Bohla, A., and Garrett, L. (2014). Pleiotropic functions for transcription factor zscan10. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104568"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.ydbio.2009.12.001","article-title":"Initiation of neuronal differentiation requires PI3-kinase\/TOR signalling in the vertebrate neural tube","volume":"338","author":"Fishwick","year":"2010","journal-title":"Dev. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.3389\/fnins.2019.01022","article-title":"Negative Regulation of mTOR Signaling Restricts Cell Proliferation in the Floor Plate","volume":"13","author":"Kadoya","year":"2019","journal-title":"Front Neurosci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.ydbio.2006.11.030","article-title":"Target of rapamycin (TOR) signaling controls epithelial morphogenesis in the vertebrate intestine","volume":"303","author":"Makky","year":"2007","journal-title":"Dev. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"7628","DOI":"10.1523\/JNEUROSCI.0726-16.2016","article-title":"Cholesterol Biosynthesis Supports Myelin Gene Expression and Axon Ensheathment through Modulation of P13K\/Akt\/mTor Signaling","volume":"36","author":"Mathews","year":"2016","journal-title":"J. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S1937-6448(10)83003-5","article-title":"Axonal ensheathment and intercellular barrier formation in Drosophila","volume":"283","author":"Blauth","year":"2010","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1002\/glia.20723","article-title":"Development of the Schwann cell lineage: From the neural crest to the myelinated nerve","volume":"56","author":"Woodhoo","year":"2008","journal-title":"Glia"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"21040","DOI":"10.1073\/pnas.1012248107","article-title":"Neural crest origin of olfactory ensheathing glia","volume":"107","author":"Barraud","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1749-8104-7-23","article-title":"Postembryonic neuronal addition in zebrafish dorsal root ganglia is regulated by Notch signaling","volume":"7","author":"McGraw","year":"2012","journal-title":"Neural. Dev."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Marchi, D., Santhakumar, K., Markham, E., Li, N., Storbeck, K.H., Krone, N., Cunliffe, V.T., and van Eeden, F.J.M. (2020). Bidirectional crosstalk between Hypoxia-Inducible Factor and glucocorticoid signalling in zebrafish larvae. PLoS Genet., 16.","DOI":"10.1371\/journal.pgen.1008757"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1042\/BJ20042121","article-title":"Identification of a functional hypoxia-responsive element that regulates the expression of the egl nine homologue 3 (egln3\/phd3) gene","volume":"390","author":"Pescador","year":"2005","journal-title":"Biochem. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/278737a0","article-title":"Direct observation of a free radical interaction between vitamin E and vitamin C","volume":"278","author":"Packer","year":"1979","journal-title":"Nature"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2174\/138920211796429736","article-title":"The genetics of vitamin C loss in vertebrates","volume":"12","author":"Drouin","year":"2011","journal-title":"Curr. Genom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1203S","DOI":"10.1093\/ajcn\/54.6.1203s","article-title":"Molecular basis for the deficiency in humans of gulonolactone oxidase, a key enzyme for ascorbic acid biosynthesis","volume":"54","author":"Nishikimi","year":"1991","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1017\/S0007114515000690","article-title":"Distribution of vitamin C is tissue specific with early saturation of the brain and adrenal glands following differential oral dose regimens in guinea pigs","volume":"113","author":"Hasselholt","year":"2015","journal-title":"Br. J. Nutr."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tveden-Nyborg, P., Vogt, L., Schjoldager, J.G., Jeannet, N., Hasselholt, S., Paidi, M.D., Christen, S., and Lykkesfeldt, J. (2012). Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0048488"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.bbrc.2011.09.146","article-title":"Differential regulation of the ascorbic acid transporter SVCT2 during development and in response to ascorbic acid depletion","volume":"414","author":"Meredith","year":"2011","journal-title":"Biochem. Biophys Res. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1158\/0008-5472.CAN-18-2070","article-title":"Collagen Prolyl Hydroxylation-Dependent Metabolic Perturbation Governs Epigenetic Remodeling and Mesenchymal Transition in Pluripotent and Cancer Cells","volume":"79","author":"Cermola","year":"2019","journal-title":"Cancer Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.semcdb.2018.10.002","article-title":"Fishing for collagen function: About development, regeneration and disease","volume":"89","author":"Bretaud","year":"2019","journal-title":"Sem. Cell Dev. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1006\/dbio.2001.0467","article-title":"Roles for zebrafish focal adhesion kinase in notochord and somite morphogenesis","volume":"240","author":"Henry","year":"2001","journal-title":"Dev. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s12929-015-0209-0","article-title":"Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development","volume":"22","author":"Li","year":"2015","journal-title":"J. Biomed. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1093\/toxsci\/kfy115","article-title":"Ancestral TCDD Exposure Induces Multigenerational Histologic and Transcriptomic Alterations in Gonads of Male Zebrafish","volume":"164","author":"Meyer","year":"2018","journal-title":"Toxicol. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1080\/01677063.2017.1358268","article-title":"Neurotoxicity of cGMP in the vertebrate retina: From the initial research on rd mutant mice to zebrafish genetic approaches","volume":"31","author":"Iribarne","year":"2017","journal-title":"J. Neurogenet"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jns.2014.07.001","article-title":"Retinitis pigmentosa and macular degeneration in a patient with ataxia with isolated vitamin E deficiency with a novel c.717 del C mutation in the TTPA gene","volume":"345","author":"Iwasa","year":"2014","journal-title":"J. Neurol. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.freeradbiomed.2018.02.037","article-title":"An innate immune response and altered nuclear receptor activation defines the spinal cord transcriptome during alpha-tocopherol deficiency in Ttpa-null mice","volume":"120","author":"Finno","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.isci.2019.10.064","article-title":"Single-Cell RNA-seq Reveals Profound Alterations in Mechanosensitive Dorsal Root Ganglion Neurons with Vitamin E Deficiency","volume":"21","author":"Finno","year":"2019","journal-title":"iScience"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1006\/bbrc.2001.5171","article-title":"Effect of selenium and vitamin E deficiency on differential gene expression in rat liver","volume":"285","author":"Fischer","year":"2001","journal-title":"Biochem. Biophys Res. Commun."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1016\/S0891-5849(03)00509-4","article-title":"Gene expression profile of oxidant stress and neurodegeneration in transgenic mice deficient in alpha-tocopherol transfer protein","volume":"35","author":"Gohil","year":"2003","journal-title":"Free Radic. Biol. Med."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/2\/468\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:17:36Z","timestamp":1760159856000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/2\/468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,30]]},"references-count":67,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["nu13020468"],"URL":"https:\/\/doi.org\/10.3390\/nu13020468","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,30]]}}}