{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:16:42Z","timestamp":1778822202997,"version":"3.51.4"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T00:00:00Z","timestamp":1615680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2018\/31\/N\/NZ7\/03680"],"award-info":[{"award-number":["2018\/31\/N\/NZ7\/03680"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>(1) The study aimed to investigate whether vitamin D3 supplementation would positively affect rats with glucocorticoids-induced muscle atrophy as measured by skeletal muscle mass in two experimental conditions: chronic dexamethasone (DEX) administration and a model of the chronic stress response. (2) The study lasted 28 consecutive days and was performed on 45 male Wistar rats randomly divided into six groups. These included two groups treated by abdominal injection of DEX at a dose of 2 mg\/kg\/day supplemented with vegetable oil (DEX PL; n = 7) or with vitamin D3 600 IU\/kg\/day (DEX SUP; n = 8), respectively, and a control group treated with an abdominal injection of saline (CON; n = 6). In addition, there were two groups of rats chronically stressed by cold water immersion (1 hour\/day in a glass box with 1-cm-deep ice\/water mixture; temperature ~4 \u00b0C), which were supplemented with vegetable oil as a placebo (STR PL; n = 9) or vitamin D3 at 600 IU\/kg\/day (STR SUP; n = 9). The last group was of sham-stressed rats (SHM; n = 6). Blood, soleus, extensor digitorum longus, gastrocnemius, tibialis anterior, and quadriceps femoris muscles were collected and weighed. The heart, liver, spleen, and thymus were removed and weighed immediately after sacrifice. The plasma corticosterone (CORT) and vitamin D3 metabolites were measured. (3) We found elevated CORT levels in both cold water-immersed groups; however, they did not alter body and muscle weight. Body weight and muscle loss occurred in groups with exogenously administered DEX, with the exception of the soleus muscle in rats supplemented with vitamin D3. Decreased serum 25(OH)D3 concentrations in DEX-treated rats were observed, and the cold water immersion did not affect vitamin D3 levels. (4) Our results indicate that DEX-induced muscle loss was abolished in rats supplemented with vitamin D3, especially in the soleus muscle.<\/jats:p>","DOI":"10.3390\/nu13030936","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T23:52:06Z","timestamp":1615765926000},"page":"936","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The Positive Impact of Vitamin D on Glucocorticoid-Dependent Skeletal Muscle Atrophy"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3301-0446","authenticated-orcid":false,"given":"Mateusz Jakub","family":"Karnia","sequence":"first","affiliation":[{"name":"Department of Physiology and Biochemistry, Gdansk University of Physical Education and Sport, Kazimierza G\u00f3rskiego 1, 80-336 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1298-4177","authenticated-orcid":false,"given":"Daria","family":"Korewo","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biochemistry, Gdansk University of Physical Education and Sport, Kazimierza G\u00f3rskiego 1, 80-336 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"My\u015bli\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziemowit Maciej","family":"Ciepielewski","sequence":"additional","affiliation":[{"name":"Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Puchalska","sequence":"additional","affiliation":[{"name":"Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaudia","family":"Konieczna-Wolska","sequence":"additional","affiliation":[{"name":"Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konrad","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Masdiag-Diagnostic Mass Spectrometry Laboratory, Stefana \u017beromskiego 33, 01-882 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan Jacek","family":"Kaczor","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biochemistry, Gdansk University of Physical Education and Sport, Kazimierza G\u00f3rskiego 1, 80-336 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.mce.2013.03.003","article-title":"Metabolic functions of glucocorticoid receptor in skeletal muscle","volume":"380","author":"Kuo","year":"2013","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_2","first-page":"55","article-title":"How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions","volume":"21","author":"Sapolsky","year":"2000","journal-title":"Endocr. Rev."},{"key":"ref_3","first-page":"447","article-title":"A meta-analysis of glucocorticoids as modulators of oxidative stress in vertebrates","volume":"181","author":"Costantini","year":"2011","journal-title":"J. Comp. Physiol. Biochem. Syst. Environ. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"12242","DOI":"10.1038\/s41598-020-69021-y","article-title":"Vitamin D protects against immobilization-induced muscle atrophy via neural crest-derived cells in mice","volume":"10","author":"Nakamura","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.nut.2018.09.031","article-title":"Vitamin D, muscle recovery, sarcopenia, cachexia, and muscle atrophy","volume":"60","author":"Garcia","year":"2019","journal-title":"Nutrition"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.jocd.2015.04.015","article-title":"Vitamin D and Sarcopenia\/Falls","volume":"18","author":"Lappe","year":"2015","journal-title":"J. Clin. Densitom. Off. J. Int. Soc. Clin. Densitom."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3177\/jnsv.64.229","article-title":"Vitamin D Attenuates FOXO1-Target Atrophy Gene Expression in C2C12 Muscle Cells","volume":"64","author":"Hirose","year":"2018","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1080\/1028415X.2017.1348436","article-title":"Oxidative stress-induced cognitive impairment in obesity can be reversed by vitamin D administration in rats","volume":"21","author":"Hajiluian","year":"2018","journal-title":"Nutr. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jsbmb.2015.05.012","article-title":"Vitamin D treatment protects against and reverses oxidative stress induced muscle proteolysis","volume":"152","author":"Bhat","year":"2015","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6835341","DOI":"10.1155\/2019\/6835341","article-title":"Vitamin D Deficiency Is Associated with Muscle Atrophy and Reduced Mitochondrial Function in Patients with Chronic Low Back Pain","volume":"2019","author":"Dzik","year":"2019","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s00421-017-3755-1","article-title":"Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain","volume":"118","author":"Dzik","year":"2018","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4336","DOI":"10.1210\/jc.2014-1742","article-title":"The effects of vitamin D on skeletal muscle strength, muscle mass, and muscle power: A systematic review and meta-analysis of randomized controlled trials","volume":"99","author":"Beaudart","year":"2014","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_13","first-page":"859","article-title":"Effect of vitamin D supplementation on muscle strength: A systematic review and meta-analysis","volume":"22","author":"Stockton","year":"2011","journal-title":"Osteoporos. Int."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Giustina, A., and Bilezikian, J.P. (2018). Vitamin D in Clinical Medicine; Front Hor Res, Karger.","DOI":"10.1159\/isbn.978-3-318-06339-4"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00774-015-0664-4","article-title":"Effects of eldecalcitol on bone and skeletal muscles in glucocorticoid-treated rats","volume":"34","author":"Kinoshita","year":"2016","journal-title":"J. Bone Miner. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"329","DOI":"10.2220\/biomedres.31.329","article-title":"Effects of a vitamin D analog, alfacalcidol, on bone and skeletal muscle in glucocorticoid-treated rats","volume":"31","author":"Miyakoshi","year":"2010","journal-title":"Biomed Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1038\/oby.2005.75","article-title":"1,25-dihydroxyvitamin D3 modulation of adipocyte glucocorticoid function","volume":"13","author":"Morris","year":"2005","journal-title":"Obes. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1038\/sj.ijo.0803294","article-title":"Dietary calcium regulates ROS production in aP2-agouti transgenic mice on high-fat\/high-sucrose diets","volume":"30","author":"Sun","year":"2006","journal-title":"Int. J. Obes."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1007\/s10495-016-1261-2","article-title":"Calcitriol-copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular carcinoma","volume":"21","author":"Rizvi","year":"2016","journal-title":"Apoptosis Int. J. Program. Cell Death"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.1210\/jc.2011-1600","article-title":"Association of glucocorticoid use and low 25-hydroxyvitamin D levels: Results from the National Health and Nutrition Examination Survey (NHANES): 2001-2006","volume":"96","author":"Skversky","year":"2011","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/nyas.12432","article-title":"Vitamin D, steroid hormones, and autoimmunity","volume":"1317","author":"Cutolo","year":"2014","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1186\/s13075-018-1706-9","article-title":"1,25(OH)2D3 and dexamethasone additively suppress synovial fibroblast activation by CCR6(+) T helper memory cells and enhance the effect of tumor necrosis factor alpha blockade","volume":"20","author":"Dankers","year":"2018","journal-title":"Arthritis Res. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"113529","DOI":"10.1016\/j.jpba.2020.113529","article-title":"Improved sample preparation method for fast LC-MS\/MS analysis of vitamin D metabolites in serum","volume":"190","author":"Rola","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s00421-019-04104-x","article-title":"Mechanisms of vitamin D on skeletal muscle function: Oxidative stress, energy metabolism and anabolic state","volume":"119","author":"Dzik","year":"2019","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Karnia, M.J., Myslinska, D., Dzik, K.P., Flis, D.J., Podlacha, M., and Kaczor, J.J. (2020). BST Stimulation Induces Atrophy and Changes in Aerobic Energy Metabolism in Rat Skeletal Muscles-The Biphasic Action of Endogenous Glucocorticoids. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082787"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4671213","DOI":"10.1155\/2018\/4671213","article-title":"The Electrical Stimulation of the Bed Nucleus of the Stria Terminalis Causes Oxidative Stress in Skeletal Muscle of Rats","volume":"2018","author":"Karnia","year":"2018","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1210\/EN.2015-1018","article-title":"Effects of Acupuncture, RU-486 on the Hypothalamic-Pituitary-Adrenal Axis in Chronically Stressed Adult Male Rats","volume":"156","author":"Eshkevari","year":"2015","journal-title":"Endocrinology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3109\/19396368.2015.1008071","article-title":"Oxidative status in testis and epididymal sperm parameters after acute and chronic stress by cold-water immersion in the adult rat","volume":"61","year":"2015","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Nishida, H., Ikegami, A., Kaneko, C., Kakuma, H., Nishi, H., Tanaka, N., Aoyama, M., Usami, M., and Okimura, Y. (2015). Dexamethasone and BCAA Failed to Modulate Muscle Mass and mTOR Signaling in GH-Deficient Rats. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0128805"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1002\/jbmr.2979","article-title":"Vitamin D Storage in Adipose Tissue of Obese and Normal Weight Women","volume":"32","author":"Carrelli","year":"2017","journal-title":"J. Bone Miner. Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, X., Wei, D., Song, Z., Jiao, H., and Lin, H. (2012). Effects of fatty acid treatments on the dexamethasone-induced intramuscular lipid accumulation in chickens. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036663"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.cmet.2017.12.004","article-title":"An Adipose Tissue Atlas: An Image-Guided Identification of Human-like BAT and Beige Depots in Rodents","volume":"27","author":"Zhang","year":"2018","journal-title":"Cell Metab."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Selye, H. (1936). A Syndrome produced by Diverse Nocuous Agents. Nature, 138.","DOI":"10.1038\/138032a0"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1152\/japplphysiol.00704.2009","article-title":"Time course expression of Foxo transcription factors in skeletal muscle following corticosteroid administration","volume":"108","author":"Cho","year":"2010","journal-title":"J. Appl. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1152\/jappl.1990.69.3.1058","article-title":"Glucocorticoid-induced muscle atrophy prevention by exercise in fast-twitch fibers","volume":"69","author":"Falduto","year":"1990","journal-title":"J. Appl. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1002\/mus.24906","article-title":"High-intensity resistance training attenuates dexamethasone-induced muscle atrophy","volume":"53","author":"Krug","year":"2016","journal-title":"Muscle Nerve"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"527","DOI":"10.3389\/fphar.2019.00527","article-title":"The Preoperative Supplementation With Vitamin D Attenuated Pain Intensity and Reduced the Level of Pro-inflammatory Markers in Patients After Posterior Lumbar Interbody Fusion","volume":"10","author":"Krasowska","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Manoy, P., Yuktanandana, P., Tanavalee, A., Anomasiri, W., Ngarmukos, S., Tanpowpong, T., and Honsawek, S. (2017). Vitamin D Supplementation Improves Quality of Life and Physical Performance in Osteoarthritis Patients. Nutrients, 9.","DOI":"10.3390\/nu9080799"},{"key":"ref_39","first-page":"CD010899","article-title":"Interventions to prevent and treat corticosteroid-induced osteoporosis and prevent osteoporotic fractures in Duchenne muscular dystrophy","volume":"1","author":"Bell","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_40","first-page":"CD008422","article-title":"Vitamin D for the management of multiple sclerosis","volume":"9","author":"Jagannath","year":"2018","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"L941","DOI":"10.1152\/ajplung.00186.2020","article-title":"Vitamin D supplementation as a rational pharmacological approach in the COVID-19 pandemic","volume":"319","author":"Ferder","year":"2020","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S2213-2600(19)30417-5","article-title":"Dexamethasone treatment for the acute respiratory distress syndrome: A multicentre, randomised controlled trial","volume":"8","author":"Villar","year":"2020","journal-title":"Lancet. Respir. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1002\/phar.1037","article-title":"Vitamin D supplementation: What\u2019s known, what to do, and what\u2019s needed","volume":"32","author":"Haines","year":"2012","journal-title":"Pharmacotherapy"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"550","DOI":"10.3389\/fendo.2018.00550","article-title":"Vitamin D Toxicity-A Clinical Perspective","volume":"9","author":"Lukaszkiewicz","year":"2018","journal-title":"Front. Endocrinol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e039935","DOI":"10.1136\/bmjopen-2020-039935","article-title":"Protocol for a case-control study of vitamin D status, adult multidrug-resistant tuberculosis disease and tuberculosis infection in Mumbai, India","volume":"10","author":"Mistry","year":"2020","journal-title":"BMJ Open"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"14985","DOI":"10.1038\/s41598-018-33248-7","article-title":"Comparative transcriptomic profile of tolerogenic dendritic cells differentiated with vitamin D3, dexamethasone and rapamycin","volume":"8","author":"Mansilla","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1007\/s00432-016-2325-y","article-title":"Influence of vitamin D signaling on hormone receptor status and HER2 expression in breast cancer","volume":"143","author":"Zhang","year":"2017","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.jsbmb.2010.03.081","article-title":"Glucocorticoid regulation of the vitamin D receptor","volume":"121","author":"Hidalgo","year":"2010","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1007\/s00394-014-0778-9","article-title":"1,25-Dihydroxyvitamin D3\/vitamin D receptor suppresses brown adipocyte differentiation and mitochondrial respiration","volume":"54","author":"Ricciardi","year":"2015","journal-title":"Eur. J. Nutr."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1111\/exd.12761","article-title":"Mitochondrial and lipogenic effects of vitamin D on differentiating and proliferating human keratinocytes","volume":"24","author":"Consiglio","year":"2015","journal-title":"Exp. Dermatol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"C536","DOI":"10.1152\/ajpcell.00568.2019","article-title":"The vitamin D receptor regulates mitochondrial function in C2C12 myoblasts","volume":"318","author":"Ashcroft","year":"2020","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"14544","DOI":"10.1074\/jbc.M112.427054","article-title":"Vitamin D enhances glucocorticoid action in human monocytes: Involvement of granulocyte-macrophage colony-stimulating factor and mediator complex subunit 14","volume":"288","author":"Zhang","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8239","DOI":"10.1038\/s41598-020-65067-0","article-title":"Regulation of vitamin D system in skeletal muscle and resident myogenic stem cell during development, maturation, and ageing","volume":"10","author":"Srikuea","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"101059","DOI":"10.1016\/j.molmet.2020.101059","article-title":"Overexpression of the vitamin D receptor (VDR) induces skeletal muscle hypertrophy","volume":"42","author":"Bass","year":"2020","journal-title":"Mol. Metab."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/3\/936\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:35:30Z","timestamp":1760160930000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/3\/936"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,14]]},"references-count":54,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["nu13030936"],"URL":"https:\/\/doi.org\/10.3390\/nu13030936","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,14]]}}}