{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T19:10:43Z","timestamp":1770837043896,"version":"3.50.1"},"reference-count":66,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T00:00:00Z","timestamp":1615852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Union for the promotion of Oil and Protein Plants (UFOP) e.V.","award":["N.A."],"award-info":[{"award-number":["N.A."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The addition of plant oils such as soybean oil (S) to a diet rich in saturated fatty acids is discussed as a possible route to prevent or diminish the development of metabolic disease. Here, we assessed whether a butterfat-rich diet fortified with S affects the development of early non-alcoholic steatohepatitis (NASH) and glucose intolerance. Female C57BL\/6J mice were fed a standard-control diet (C); a fat-, fructose-, and cholesterol-rich diet (FFC, 25E% butterfat, 50% (wt.\/wt.) fructose, 0.16% (wt.\/wt.) cholesterol); or FFC supplemented with S (FFC + S, 21E% butterfat + 4E% S) for 13 weeks. Indicators of liver damage, inflammation, intestinal barrier function, and glucose metabolism were measured. Lipopolysaccharide (LPS)-challenged J774A.1 cells were incubated with linolenic and linoleic acids (ratio 1:7.1, equivalent to S). The development of early NASH and glucose intolerance was significantly attenuated in FFC + S\u2013fed mice compared to FFC-fed mice associated with lower hepatic toll-like receptor-4 mRNA expression, while markers of intestinal barrier function were significantly higher than in C-fed mice. Linolenic and linoleic acid significantly attenuated LPS-induced formation of reactive nitrogen species and interleukin-1 beta mRNA expression in J774A.1 cells. Our results indicate that fortifying butterfat with S may attenuate the development of NASH and glucose intolerance in mice.<\/jats:p>","DOI":"10.3390\/nu13030959","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T12:01:02Z","timestamp":1615896062000},"page":"959","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Fortifying Butterfat with Soybean Oil Attenuates the Onset of Diet-Induced Non-Alcoholic Steatohepatitis and Glucose Intolerance"],"prefix":"10.3390","volume":"13","author":[{"given":"Victor","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Brandt","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7235-0382","authenticated-orcid":false,"given":"Cheng Jun","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Nutrition, SD Model Systems of Molecular Nutrition, Friedrich-Schiller University of Jena, Dornburger Stra\u00dfe 25-29, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6844-8878","authenticated-orcid":false,"given":"Dragana","family":"Rajcic","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Janina","family":"Engstler","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8513-140X","authenticated-orcid":false,"given":"Finn","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anika","family":"Nier","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Baumann","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ina","family":"Bergheim","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstra\u00dfe 14\/UZAII, A-1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1053\/jhep.2002.30690","article-title":"Nonalcoholic steatohepatitis, insulin resistance, and metabolic syndrome: Further evidence for an etiologic association","volume":"35","author":"Pagano","year":"2002","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.2174\/1570161115666170621075619","article-title":"Is nonalcoholic fatty liver disease indeed the hepatic manifestation of metabolic syndrome?","volume":"16","author":"Katsiki","year":"2018","journal-title":"Curr. Vasc. 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