{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:24:47Z","timestamp":1790742287468,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Finance Forestry Technology Promotion Demonstration Project","award":["SLTG[2021]04"],"award-info":[{"award-number":["SLTG[2021]04"]}]},{"name":"Shaanxi Province Agricultural Industrialization Development Project in 2020","award":["None"],"award-info":[{"award-number":["None"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>As a natural active substance that can effectively improve blood lipid balance in the body, hypolipidemic active peptides have attracted the attention of scholars. In this study, the effect of walnut meal peptides (WMP) on lipid metabolism was investigated in rats fed a high-fat diet (HFD). The experimental results show that feeding walnut meal peptides counteracted the high-fat diet-induced increase in body, liver and epididymal fat weight, and reduce the serum concentrations of total cholesterol, triglycerides, and LDL-cholesterol and hepatic cholesterol and triglyceride content. Walnut meal peptides also resulted in increased HDL-cholesterol while reducing the atherosclerosis index (AI). Additionally, the stained pathological sections of the liver showed that the walnut meal peptides reduced hepatic steatosis and damage caused by HFD. Furthermore, walnut meal peptide supplementation was associated with normalization of elevated apolipoprotein (Apo)-B and reduced Apo-A1 induced by the high-fat diet and with favorable changes in the expression of genes related to lipid metabolism (LCAT, CYP7A1, HMGR, FAS). The results indicate that walnut meal peptides can effectively prevent the harmful effects of a high-fat diet on body weight, lipid metabolism and liver fat content in rats, and provide, and provide a reference for the further development of walnut meal functional foods.<\/jats:p>","DOI":"10.3390\/nu13051410","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T13:59:14Z","timestamp":1619099954000},"page":"1410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Effect of Walnut Meal Peptides on Hyperlipidemia and Hepatic Lipid Metabolism in Rats Fed a High-Fat Diet"],"prefix":"10.3390","volume":"13","author":[{"given":"Xiao-Yue","family":"Yang","sequence":"first","affiliation":[{"name":"College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi\u2019an 710119, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di-Ying","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi\u2019an 710119, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Liang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi\u2019an 710119, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run-Guang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi\u2019an 710119, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5881-2018","authenticated-orcid":false,"given":"You-Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi\u2019an 710119, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/eurheartj\/ehz569","article-title":"Obesity causes cardiovascular diseases: Adding to the weight of evidence","volume":"41","author":"Hingorani","year":"2020","journal-title":"Eur. Hear. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"s9","DOI":"10.1210\/jc.2008-1595","article-title":"Obesity and the Metabolic Syndrome in Developing Countries","volume":"93","author":"Misra","year":"2008","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.beem.2015.04.003","article-title":"Childhood obesity: Current and novel approaches","volume":"29","author":"Sabin","year":"2015","journal-title":"Best Pr. Res. Clin. Endocrinol. Metab."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1056\/NEJMra1514009","article-title":"Mechanisms, Pathophysiology, and Management of Obesity","volume":"376","author":"Heymsfield","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2459","DOI":"10.1093\/eurheartj\/ehx144","article-title":"Low-density lipoproteins cause atherosclerotic cardiovascular disease. 1. Evidence from genetic, epidemiologic, and clinical studies. A consensus statement from the European Atherosclerosis Society Consensus Panel","volume":"38","author":"Ference","year":"2017","journal-title":"Eur. Heart J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.ejim.2015.01.005","article-title":"Drug treatment of obesity: Current status and future prospects","volume":"26","author":"Kakkar","year":"2015","journal-title":"Eur. J. Intern. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1001\/jama.2016.7602","article-title":"Association of Pharmacological Treatments for Obesity With Weight Loss and Adverse Events","volume":"315","author":"Khera","year":"2016","journal-title":"JAMA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/clpt.2013.204","article-title":"Antiobesity Pharmacotherapy: New Drugs and Emerging Targets","volume":"95","author":"Kim","year":"2013","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1111\/obr.12409","article-title":"Effects of dietary polyphenols on metabolic syndrome features in humans: A systematic review","volume":"17","author":"Amiot","year":"2016","journal-title":"Obes. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/07315724.2010.10719814","article-title":"Green Tea Supplementation Affects Body Weight, Lipids, and Lipid Peroxidation in Obese Subjects with Metabolic Syndrome","volume":"29","author":"Basu","year":"2010","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev-nutr-071715-050718","article-title":"Citrus Flavonoids as Regulators of Lipoprotein Metabolism and Atherosclerosis","volume":"36","author":"Mulvihill","year":"2016","journal-title":"Annu. Rev. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1194\/jlr.M087387","article-title":"Intervention with citrus flavonoids reverses obesity and improves metabolic syndrome and atherosclerosis in obese Ldlr\u2212\/\u2212 mice","volume":"59","author":"Burke","year":"2018","journal-title":"J. Lipid Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1039\/C4FO00970C","article-title":"The anti-obesity effect of green tea polysaccharides, polyphenols and caffeine in rats fed with a high-fat diet","volume":"6","author":"Xu","year":"2015","journal-title":"Food Funct."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Friedman, M. (2016). Mushroom Polysaccharides: Chemistry and Antiobesity, Antidiabetes, Anticancer, and Antibiotic Properties in Cells, Rodents, and Humans. Foods, 5.","DOI":"10.3390\/foods5040080"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/1541-4337.12402","article-title":"Meta-Analysis for Correlating Structure of Bioactive Peptides in Foods of Animal Origin with Regard to Effect and Stability","volume":"18","author":"Maestri","year":"2019","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Chatterjee, C., Gleddie, S., and Xiao, C.-W. (2018). Soybean Bioactive Peptides and Their Functional Properties. Nutrients, 10.","DOI":"10.3390\/nu10091211"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/biof.5520120122","article-title":"Bioactive peptides derived from food proteins preventing lifestyle-related diseases","volume":"12","author":"Yoshikawa","year":"2000","journal-title":"BioFactors"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.foodres.2006.11.011","article-title":"Preparation of hypocholesterol peptides from soy protein and their hypocholesterolemic effect in mice","volume":"40","author":"Zhong","year":"2007","journal-title":"Food Res. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10927","DOI":"10.1021\/jf202721z","article-title":"Lower Weight Gain and Hepatic Lipid Content in Hamsters Fed High Fat Diets Supplemented with White Rice Protein, Brown Rice Protein, Soy Protein, and their Hydrolysates","volume":"59","author":"Zhang","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11351","DOI":"10.1021\/jf303471f","article-title":"Evaluation of Antihypertensive and Antihyperlipidemic Effects of Bamboo Shoot Angiotensin Converting Enzyme Inhibitory Peptide in Vivo","volume":"60","author":"Liu","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.foodchem.2014.07.049","article-title":"Peptides from cowpea present antioxidant activity, inhibit cholesterol synthesis and its solubilisation into micelles","volume":"168","author":"Marques","year":"2015","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1016\/j.jacc.2020.07.071","article-title":"Effects of 2-Year Walnut-Supplemented Diet on Inflammatory Biomarkers","volume":"76","author":"Rajaram","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.2337\/dc09-1156","article-title":"Effects of Walnut Consumption on Endothelial Function in Type 2 Diabetic Subjects: A randomized controlled crossover trial","volume":"33","author":"Ma","year":"2009","journal-title":"Diabetes Care"},{"key":"ref_24","first-page":"67","article-title":"Dietary walnuts inhibit colorectal cancer growth in mice by suppressing angiogenesis","volume":"28","author":"Nagel","year":"2012","journal-title":"Nutrients"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1093\/nutrit\/nuz042","article-title":"Nut consumption and incidence of cardiovascular diseases and cardiovascular disease mortality: A meta-analysis of prospective cohort studies","volume":"77","author":"Kendall","year":"2019","journal-title":"Nutr. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1093\/ajcn\/nqy091","article-title":"Effects of walnut consumption on blood lipids and other cardiovascular risk factors: An updated meta-analysis and systematic review of controlled trials","volume":"108","author":"Li","year":"2018","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.foodchem.2009.12.027","article-title":"Influence of degree of hydrolysis on functional properties, antioxidant activity and ACE inhibitory activity of peanut protein hydrolysate","volume":"121","author":"Jamdar","year":"2010","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4097","DOI":"10.1021\/jf4001378","article-title":"Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein","volume":"61","author":"Liu","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1080\/10942912.2012.716476","article-title":"Separation, Purification, and Identification of Angiotensin I\u2013Converting Enzyme Inhibitory Peptides from Walnut (Juglans regia L.) Hydrolyzate","volume":"18","author":"Gu","year":"2015","journal-title":"Int. J. Food Prop."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"575","DOI":"10.4012\/dmj.2011-209","article-title":"Effects of different luting cements and light curing units on the sealing ability and bond strength of fiber posts","volume":"31","author":"Beriat","year":"2012","journal-title":"Dent. Mater. J."},{"key":"ref_31","unstructured":"Mao, X.Y. (2012). Studies on the Structure Characterization and PRODUCTS\u2019 modification of Walnut Protein, University of Jiang Nan."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1006\/bbrc.2001.4298","article-title":"Identification of Novel Hypocholesterolemic Peptides Derived from Bovine Milk \u03b2-Lactoglobulin","volume":"281","author":"Nagaoka","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.foodres.2018.05.009","article-title":"Isolation and identification by high resolution liquid chromatography tandem mass spectrometry of novel peptides with multifunctional lipid-lowering capacity","volume":"111","author":"Prados","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s10534-010-9298-0","article-title":"Probiotic Lactobacillus casei Expressing Human Lactoferrin Elevates Antibacterial Activity in the Gastrointestinal Tract","volume":"23","author":"Chen","year":"2010","journal-title":"BioMetals"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1515\/bchm2.1951.286.1-6.270","article-title":"\u00dcber die Einwirkung von naszierender Salpetriger S\u00e4ure auf verschiedene stickstoffhaltige organische Verbindungen. (Zur van Slykeschen Aminostickstoffbestimmung)","volume":"286","author":"Schenck","year":"1951","journal-title":"Hoppe-Seyler\u00b4s Zeitschrift f\u00fcr Physiologische Chemie"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0271-5317(95)00036-I","article-title":"Hypocholesterolemic effect of buckwheat protein extract in rats fed cholesterol enriched diets","volume":"15","author":"Kayashita","year":"1995","journal-title":"Nutr. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e9757","DOI":"10.7717\/peerj.9757","article-title":"Gender-specific associations between apolipoprotein A1 and arterial stiffness in patients with nonalcoholic fatty liver disease","volume":"8","author":"Sun","year":"2020","journal-title":"PeerJ"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"145","DOI":"10.14740\/jocmr2030w","article-title":"Effects of Dietary Fat Intake on HDL Metabolism","volume":"7","author":"Yanai","year":"2015","journal-title":"J. Clin. Med. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1111\/jgh.12077","article-title":"Apolipoprotein B\/AI ratio is independently associated with non-alcoholic fatty liver disease in nondiabetic subjects","volume":"28","author":"Jin","year":"2013","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1161\/ATVBAHA.111.241471","article-title":"Intracellular Trafficking and Secretion of VLDL","volume":"32","author":"Tiwari","year":"2012","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8015","DOI":"10.1021\/acs.jafc.8b02979","article-title":"Pectin Alleviates High Fat (Lard) Diet-Induced Nonalcoholic Fatty Liver Disease in Mice: Possible Role of Short-Chain Fatty Acids and Gut Microbiota Regulated by Pectin","volume":"66","author":"Li","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ijbiomac.2020.06.241","article-title":"Dietary quinoa (Chenopodium quinoa Willd.) polysaccharides ameliorate high-fat diet-induced hyperlipidemia and modulate gut microbiota","volume":"163","author":"Cao","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3661","DOI":"10.1021\/jf021156g","article-title":"Antioxidant Activity of Peptides Obtained from Porcine Myofibrillar Proteins by Protease Treatment","volume":"51","author":"Saiga","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.foodchem.2007.11.010","article-title":"Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry","volume":"108","author":"Ren","year":"2008","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/j.foodchem.2017.04.071","article-title":"Fractionation and identification of novel antioxidant peptides from buffalo and bovine casein hydrolysates","volume":"232","author":"Shazly","year":"2017","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1021\/jf00013a003","article-title":"Sequence-dependent reactivity of histidine-containing peptides with copper(II)\/ascorbate","volume":"40","author":"Uchida","year":"1992","journal-title":"J. Agric. Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.wem.2020.06.010","article-title":"L-Arginine Supplementation Improves Vascular Endothelial Dysfunction Induced by High-Fat Diet in Rats Exposed to Hypoxia","volume":"31","author":"Zhao","year":"2020","journal-title":"Wilderness Environ. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0021-9258(18)70949-6","article-title":"The digestion in vitro of triglycerides by pancreatic lipase","volume":"214","author":"Mattson","year":"1955","journal-title":"J. Biol. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1097\/FJC.0b013e3181feb90a","article-title":"High-carbohydrate, High-fat Diet\u2013induced Metabolic Syndrome and Cardiovascular Remodeling in Rats: Erratum","volume":"57","author":"Panchal","year":"2011","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ijbiomac.2020.06.055","article-title":"The protective effects of walnut green husk polysaccharide on liver injury, vascular endothelial dysfunction and disorder of gut microbiota in high fructose-induced mice","volume":"162","author":"Wang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6971","DOI":"10.1039\/D0FO01282C","article-title":"Fubrick tea attenuates high-fat diet induced fat deposition and metabolic disorder by regulating gut microbiota and caffeine metabolism","volume":"11","author":"Jing","year":"2020","journal-title":"Food Funct."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.1039\/C9FO00075E","article-title":"Grifola frondosapolysaccharides ameliorate lipid metabolic disorders and gut microbiota dysbiosis in high-fat diet fed rats","volume":"10","author":"Li","year":"2019","journal-title":"Food Funct."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.plipres.2012.11.002","article-title":"Cholesterol metabolism and the pathogenesis of non-alcoholic steatohepatitis","volume":"52","author":"Musso","year":"2013","journal-title":"Prog. Lipid Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jff.2019.04.049","article-title":"Spirulina active substance mediated gut microbes improve lipid metabolism in high-fat diet fed rats","volume":"59","author":"Chen","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1161\/01.ATV.16.2.294","article-title":"Two Novel Molecular Defects in theLCATGene Are Associated With Fish Eye Disease","volume":"16","author":"Kuivenhoven","year":"1996","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"967","DOI":"10.2174\/092986706776361012","article-title":"Inhibition of Fatty Acid Synthase by Polyphenols","volume":"13","author":"Tian","year":"2006","journal-title":"Curr. Med. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jff.2016.12.023","article-title":"Ginger extract increases muscle mitochondrial biogenesis and serum HDL-cholesterol level in high-fat diet-fed rats","volume":"29","author":"Oh","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"108816","DOI":"10.1016\/j.foodres.2019.108816","article-title":"Effect of embryo-remaining oat rice on the lipid profile and intestinal microbiota in high-fat diet fed rats","volume":"129","author":"Huang","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1039\/C8FO02502A","article-title":"The beneficial effects of purple yam (Dioscorea alata L.) resistant starch on hyperlipidemia in high-fat-fed hamsters","volume":"10","author":"Li","year":"2019","journal-title":"Food Funct."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1002\/em.20546","article-title":"DNA damage and toxicogenomic analyses of hydrogen sulfide in human intestinal epithelial FHs 74 Int cells","volume":"51","author":"Nava","year":"2010","journal-title":"Environ. Mol. Mutagen."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S1095-6433(02)00121-6","article-title":"Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto","volume":"133","author":"Samanya","year":"2002","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_62","first-page":"154","article-title":"Relationship between hyperlipidemia and the gut microbiome of rats, characterized using high-throughput sequencing","volume":"7","author":"Li","year":"2020","journal-title":"J. Tradit. Chin. Med Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.carbpol.2016.10.042","article-title":"Enhanced anti-obesity effects of complex of resistant starch and chitosan in high fat diet fed rats","volume":"157","author":"Si","year":"2017","journal-title":"Carbohydr. Polym."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/5\/1410\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:51:28Z","timestamp":1760161888000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/5\/1410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,22]]},"references-count":63,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["nu13051410"],"URL":"https:\/\/doi.org\/10.3390\/nu13051410","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,22]]}}}