{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:33:01Z","timestamp":1791923581733,"version":"4.3.4"},"reference-count":98,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T00:00:00Z","timestamp":1621036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This research was funded by KNOW (Leading National Research Centre) Scientific Consortium &quot;Healthy Animal - Safe Food&quot;, the decision of Ministry of Science and Higher Education No. 05-1\/KNOW2\/2015\u201d","award":["KNOW2015\/CB\/PRO1\/44."],"award-info":[{"award-number":["KNOW2015\/CB\/PRO1\/44."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n\u20136) to \u03b1-linolenic acid (ALA, C18:3n\u20133) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3\u201310 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF\/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (\u2193: ALB, APOA1, IVD, MAT1A, OAT and PHB; \u2191: ALDH1L1, UniProtKB\u2014Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (\u2193: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; \u2191: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (\u2193: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; \u2191: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets.<\/jats:p>","DOI":"10.3390\/nu13051678","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T02:31:34Z","timestamp":1621218694000},"page":"1678","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n\u20136) to \u03b1-Linolenic Acid (ALA, C18:3n\u20133) Ratio on the Mouse Liver Proteome"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2139-8241","authenticated-orcid":false,"given":"Kamila P.","family":"Liput","sequence":"first","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"},{"name":"Department of Molecular Biology, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Lepczy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology, K. Janickiego 32 Str., 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2806-6194","authenticated-orcid":false,"given":"Agata","family":"Nawrocka","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"},{"name":"Department of Experimental Genomics, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Po\u0142awska","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Og\u0142uszka","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Jo\u0144czy","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weronika","family":"Grzybek","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Liput","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Bioengineering, Mossakowski Medical Research Institute of the Polish Academy of Sciences, 02-106 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Szostak","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawe\u0142","family":"Urba\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Roszczyk","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0329-787X","authenticated-orcid":false,"given":"Chandra S.","family":"Pareek","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Toru\u0144, Poland"},{"name":"Division of Functional Genomics in Biological and Biomedical Research, Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 87-100 Torun, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariusz","family":"Pierzcha\u0142a","sequence":"additional","affiliation":[{"name":"Department of Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108460","DOI":"10.1016\/j.jnutbio.2020.108460","article-title":"A saturated fatty acid-rich diet enhances hepatic lipogenesis and tumorigenesis in HCV core gene transgenic mice","volume":"85","author":"Diao","year":"2020","journal-title":"J. Nutr. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17063","DOI":"10.1038\/cddiscovery.2017.63","article-title":"The lauric acid-activated signaling prompts apoptosis in cancer cells","volume":"3","author":"Lappano","year":"2017","journal-title":"Cell Death Discov."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/fqsafe\/fyz004","article-title":"Coconut oil: What do we really know about it so far?","volume":"3","author":"Lima","year":"2019","journal-title":"Food Qual. Saf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/0026-0495(87)90005-9","article-title":"Stimulation of thermogenesis and brown fat activity in rats fed medium chain triglyceride","volume":"36","author":"Rothwell","year":"1987","journal-title":"Metabolism"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1093\/ajcn\/74.5.620","article-title":"Substrate oxidation and control of food intake in men after a fat-substitute meal compared with meals supplemented with an isoenergetic load of carbohydrate, long-chain triacylglycerols, or medium-chain triacylglycerols","volume":"74","author":"Fantino","year":"2001","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1038\/ejcn.2017.86","article-title":"They say coconut oil can aid weight loss, but can it really?","volume":"71","author":"Clegg","year":"2017","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_7","unstructured":"Department of Health and Human Services and U.S. Department of Agriculture (HHS) and (USDA) (2021, May 13). Dietary Guidelines for Americans, Available online: http:\/\/health.gov\/dietaryguidelines\/2015\/guidelines\/."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S76","DOI":"10.1161\/01.cir.0000437740.48606.d1","article-title":"2013 AHA\/ACC guideline on lifestyle management to reduce cardiovascular risk: A report of the American College of Cardiology\/American Heart Association Task Force on Practice Guidelines","volume":"129","author":"Eckel","year":"2014","journal-title":"Circulation"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1006\/pmed.1995.1049","article-title":"Dietary saturated and trans fatty acids and cholesterol and 25-year mortality from coronary heart disease: The Seven Countries Study","volume":"24","author":"Kromhout","year":"1995","journal-title":"Prev. Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Billingsley, H.E., Carbone, S., and Lavie, C.J. (2018). Dietary Fats and Chronic Noncommunicable Diseases. Nutrients, 10.","DOI":"10.3390\/nu10101385"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1001\/jamainternmed.2016.2417","article-title":"Association of Specific Dietary Fats With Total and Cause-Specific Mortality","volume":"176","author":"Wang","year":"2016","journal-title":"JAMA Intern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Gouaref, I., Bouazza, A., Abderrhmane, S.A., and Koceir, E.-A. (2020). Lipid Profile Modulates Cardiometabolic Risk Biomarkers Including Hypertension in People with Type-2 Diabetes: A Focus on Unbalanced Ratio of Plasma Polyunsaturated\/Saturated Fatty Acids. Molecules, 25.","DOI":"10.3390\/molecules25184315"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1080\/10408398.2012.724481","article-title":"Dietary fat and breast cancer mortality: A systematic review and meta-analysis","volume":"57","author":"Brennan","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.pcad.2020.08.004","article-title":"The role of diet and nutrition in heart failure: A state-of-the-art narrative review","volume":"63","author":"Billingsley","year":"2020","journal-title":"Prog. Cardiovasc. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3945\/ajcn.2009.27725","article-title":"Meta-analysis of prospective cohort studies evaluating the association of saturated fat with cardiovascular disease","volume":"91","author":"Sun","year":"2010","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Unger, A.L., Torres-Gonzalez, M., and Kraft, J. (2019). Dairy Fat Consumption and the Risk of Metabolic Syndrome: An Examination of the Saturated Fatty Acids in Dairy. Nutrients, 11.","DOI":"10.3390\/nu11092200"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Lepczy\u0144ski, A., O\u017cgo, M., Micha\u0142ek, K., Dratwa-Cha\u0142upnik, A., Grabowska, M., Herosimczyk, A., Liput, K.P., Po\u0142awska, E., Kram, A., and Pierzcha\u0142a, M. (2021). Effects of Three-Month Feeding High Fat Diets with Different Fatty Acid Composition on Myocardial Proteome in Mice. Nutrients, 13.","DOI":"10.3390\/nu13020330"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/elps.200900481","article-title":"CBB staining protocol with higher sensitivity and mass spectrometric compatibility","volume":"31","author":"Pink","year":"2010","journal-title":"Electrophoresis"},{"key":"ref_19","first-page":"67","article-title":"Analysis of the efficiency of post-electrophoretic protein staining using Colloidal Coomassie Blue G-250","volume":"14","author":"Herosimczyk","year":"2015","journal-title":"Acta Sci. Pol. Zootechnica"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"STRING v11: Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_21","first-page":"D506","article-title":"UniProt: A worldwide hub of protein knowledge","volume":"47","author":"UniProt","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_23","first-page":"D330","article-title":"The Gene Ontology Resource: 20 years and still GOing strong","volume":"47","author":"Carbon","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ygeno.2017.08.005","article-title":"pLoc-mEuk: Predict subcellular localization of multi-label eukaryotic proteins by extracting the key GO information into general PseAAC","volume":"110","author":"Cheng","year":"2018","journal-title":"Genomics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"38513","DOI":"10.1038\/srep38513","article-title":"Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restriction","volume":"6","author":"Gong","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Hruz, T., Wyss, M., Docquier, M., Pfaffl, M.W., Masanetz, S., Borghi, L., Verbrugghe, P., Kalaydjieva, L., Bleuler, S., and Laule, O. (2011). RefGenes: Identification of reliable and condition specific reference genes for RT-qPCR data normalization. BMC Genom., 12.","DOI":"10.1186\/1471-2164-12-156"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets","volume":"64","author":"Andersen","year":"2004","journal-title":"Cancer Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/gkp045","article-title":"Amplification efficiency: Linking baseline and bias in the analysis of quantitative PCR data","volume":"37","author":"Ruijter","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref_30","first-page":"86","article-title":"PCR w czasie rzeczywistym. Metody analizy danych","volume":"1","author":"Tyburski","year":"2008","journal-title":"Biotechnologia"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e11","DOI":"10.1038\/nutd.2011.7","article-title":"Differential effects of saturated versus unsaturated dietary fatty acids on weight gain and myocellular lipid profiles in mice","volume":"1","author":"Timmers","year":"2011","journal-title":"Nutr. Diabetes"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Beulen, Y., Mart\u00ednez-Gonz\u00e1lez, M.A., van de Rest, O., Salas-Salvad\u00f3, J., Sorl\u00ed, J.V., G\u00f3mez-Gracia, E., Fiol, M., Estruch, R., Santos-Lozano, J.M., and Schr\u00f6der, H. (2018). Quality of Dietary Fat Intake and Body Weight and Obesity in a Mediterranean Population: Secondary Analyses within the PREDIMED Trial. Nutrients, 10.","DOI":"10.3390\/nu10122011"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.2337\/db13-1622","article-title":"Overfeeding polyunsaturated and saturated fat causes distinct effects on liver and visceral fat accumulation in humans","volume":"63","author":"Rosqvist","year":"2014","journal-title":"Diabetes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12986-020-00456-w","article-title":"High fat diet significantly changed the global gene expression profile involved in hepatic drug metabolism and pharmacokinetic system in mice","volume":"17","author":"He","year":"2020","journal-title":"Nutr. Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21248","DOI":"10.1039\/D0RA00215A","article-title":"Sinapine-enriched rapeseed oils reduced fatty liver formation in high-fat diet-fed C57BL\/6J mice","volume":"10","author":"Li","year":"2020","journal-title":"RSC Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2414","DOI":"10.1194\/jlr.M800271-JLR200","article-title":"Replacement of dietary saturated FAs by PUFAs in diet and reverse cholesterol transport","volume":"49","author":"Suchanek","year":"2008","journal-title":"J. Lipid Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1017\/S0007114520003402","article-title":"Beneficial effect on serum cholesterol levels, but not glycaemic regulation, after replacing SFA with PUFA for 3 d: A randomised crossover trial","volume":"125","author":"Gaundal","year":"2021","journal-title":"Br. J. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.nut.2003.10.012","article-title":"Diets rich in saturated and polyunsaturated fatty acids: Metabolic shifting and cardiac health","volume":"20","author":"Diniz","year":"2004","journal-title":"Nutrition"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1016\/j.celrep.2017.11.059","article-title":"Hepatic Dysfunction Caused by Consumption of a High-Fat Diet","volume":"21","author":"Soltis","year":"2017","journal-title":"Cell Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.bbrc.2018.11.026","article-title":"Short- and long-term effects of high-fat diet feeding and voluntary exercise on hepatic lipid metabolism in mice","volume":"507","author":"Yoshimura","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1038\/ncb3551","article-title":"Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation","volume":"19","author":"Wang","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1194\/jlr.M700109-JLR200","article-title":"ACAT2 stimulates cholesteryl ester secretion in apoB-containing lipoproteins","volume":"48","author":"Temel","year":"2007","journal-title":"J. Lipid Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1161\/ATVBAHA.107.142802","article-title":"Dietary Fat\u2013Induced Alterations in Atherosclerosis Are Abolished by ACAT2-Deficiency in ApoB100 Only, LDLr\u2212\/\u2212 Mice","volume":"27","author":"Bell","year":"2007","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1074\/mcp.M113.027441","article-title":"Quantitative proteomic and functional analysis of liver mitochondria from high fat diet (HFD) diabetic mice","volume":"12","author":"Guo","year":"2013","journal-title":"Mol. Cell Proteom."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Schlune, A., Riederer, A., Mayatepek, E., and Ensenauer, R. (2018). Aspects of Newborn Screening in Isovaleric Acidemia. Int. J. Neonatal Screen., 4.","DOI":"10.3390\/ijns4010007"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1080\/10715760802311179","article-title":"Induction of oxidative stress by the metabolites accumulating in isovaleric acidemia in brain cortex of young rats","volume":"42","author":"Solano","year":"2008","journal-title":"Free Radic. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0005-2760(97)00134-3","article-title":"The lipolysis of chylomicrons derived from different dietary fats by lipoprotein lipase in vitro","volume":"1349","author":"Botham","year":"1997","journal-title":"Biochim. Biophys. Acta Lipids Lipid Metab."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1582-4934.2010.01045.x","article-title":"Apolipoprotein A-I possesses an anti-obesity effect associated with increase of energy expenditure and up-regulation of UCP1 in brown fat","volume":"15","author":"Ruan","year":"2011","journal-title":"J. Cell Mol. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e108","DOI":"10.1038\/nutd.2014.4","article-title":"Modulation of adipose tissue lipolysis and body weight by high-density lipoproteins in mice","volume":"4","author":"Wei","year":"2014","journal-title":"Nutr. Diabetes"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1007\/s00125-007-0752-7","article-title":"Apolipoprotein A-I stimulates AMP-activated protein kinase and improves glucose metabolism","volume":"50","author":"Han","year":"2007","journal-title":"Diabetologia"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"25747","DOI":"10.1038\/srep25747","article-title":"Ces3\/TGH Deficiency Attenuates Steatohepatitis","volume":"6","author":"Lian","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1002\/hep.25881","article-title":"Liver specific inactivation of carboxylesterase 3\/triacylglycerol hydrolase decreases blood lipids without causing severe steatosis in mice","volume":"56","author":"Lian","year":"2012","journal-title":"Hepatology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1017\/S0029665118002604","article-title":"Dietary sugars, exercise and hepatic carbohydrate metabolism","volume":"78","author":"Gonzalez","year":"2018","journal-title":"Proc. Nutr. Soc."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4059","DOI":"10.1172\/JCI94585","article-title":"Divergent effects of glucose and fructose on hepatic lipogenesis and insulin signaling","volume":"127","author":"Softic","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.2337\/db12-1311","article-title":"Targeting pyruvate carboxylase reduces gluconeogenesis and adiposity and improves insulin resistance","volume":"62","author":"Kumashiro","year":"2013","journal-title":"Diabetes"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1007\/s00394-016-1232-y","article-title":"Epigenetic and SP1-mediated regulation is involved in the repression of galactokinase 1 gene in the liver of neonatal piglets born to betaine-supplemented sows","volume":"56","author":"Cai","year":"2017","journal-title":"Eur. J. Nutr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1097\/MCO.0000000000000189","article-title":"Galactose metabolism and health","volume":"18","author":"Coelho","year":"2015","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_58","unstructured":"Ramani, P., and Arya, K. (2021, May 13). Galactokinase Deficiency. StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK560683\/."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Bashir, S., Sharma, Y., Jairajpuri, D., Rashid, F., Nematullah, M., and Khan, F. (2019). Alteration of adipose tissue immune cell milieu towards the suppression of inflammation in high fat diet fed mice by flaxseed oil supplementation. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0223070"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s13105-017-0597-6","article-title":"Early obesity leads to increases in hepatic arginase I and related systemic changes in nitric oxide and l-arginine metabolism in mice","volume":"74","author":"Ito","year":"2018","journal-title":"J. Physiol. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1002\/oby.20969","article-title":"Arginase promotes endothelial dysfunction and hypertension in obese rats","volume":"23","author":"Johnson","year":"2015","journal-title":"Obesity"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Erdely, A., Kepka-Lenhart, D., Salmen-Muniz, R., Chapman, R., Hulderman, T., Kashon, M., Simeonova, P.P., and Morris, S.M. (2010). Arginase activities and global arginine bioavailability in wild-type and ApoE-deficient mice: Responses to high fat and high cholesterol diets. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015253"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1161\/CIRCRESAHA.107.155028","article-title":"Diabetes-induced coronary vascular dysfunction involves increased arginase activity","volume":"102","author":"Romero","year":"2008","journal-title":"Circ. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.bbrc.2014.07.083","article-title":"Arginase inhibition restores endothelial function in diet-induced obesity","volume":"451","author":"Chung","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Moon, J., Do, H.J., Cho, Y., and Shin, M.-J. (2014). Arginase inhibition ameliorates hepatic metabolic abnormalities in obese mice. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0103048"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1743-7075-11-6","article-title":"Novel regulatory roles of omega-3 fatty acids in metabolic pathways: A proteomics approach","volume":"11","author":"Ahmed","year":"2014","journal-title":"Nutr. Metab."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"60","DOI":"10.4172\/jpb.1000214","article-title":"High fat diet-induced changes in hepatic protein abundance in mice","volume":"5","author":"Luo","year":"2012","journal-title":"J. Proteom. Bioinform."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1017\/S0007114508043389","article-title":"Overexpression of ornithine aminotransferase: Consequences on amino acid homeostasis","volume":"101","author":"Ventura","year":"2008","journal-title":"Br. J. Nutr."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"612089","DOI":"10.1155\/2010\/612089","article-title":"Peroxisome Proliferator-Activated Receptor Alpha Target Genes","volume":"2010","author":"Rakhshandehroo","year":"2010","journal-title":"PPAR Res."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Z\u00fa\u00f1iga, J., Cancino, M., Medina, F., Varela, P., Vargas, R., Tapia, G., Videla, L.A., and Fern\u00e1ndez, V. (2011). N-3 PUFA supplementation triggers PPAR-\u03b1 activation and PPAR-\u03b1\/NF-\u03baB interaction: Anti-inflammatory implications in liver ischemia-reperfusion injury. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028502"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1021\/acsmedchemlett.5b00153","article-title":"Suppression of Hepatocellular Carcinoma by Inhibition of Overexpressed Ornithine Aminotransferase","volume":"6","author":"Zigmond","year":"2015","journal-title":"ACS Med. Chem. Lett."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jnutbio.2016.12.005","article-title":"Changes in liver proteins of rats fed standard and high-fat and sucrose diets induced by fish omega-3 PUFAs and their combination with grape polyphenols according to quantitative proteomics","volume":"41","author":"Ciordia","year":"2017","journal-title":"J. Nutr. Biochem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.bbalip.2011.05.005","article-title":"Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats","volume":"1811","author":"Ciapaite","year":"2011","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5080843","DOI":"10.1155\/2019\/5080843","article-title":"Reactive Oxygen Species-Induced Lipid Peroxidation in Apoptosis, Autophagy, and Ferroptosis","volume":"2019","author":"Su","year":"2019","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1089\/ars.2010.3412","article-title":"Peroxiredoxin 6: A bifunctional enzyme with glutathione peroxidase and phospholipase A\u2082 activities","volume":"15","author":"Fisher","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"9685607","DOI":"10.1155\/2019\/9685607","article-title":"Peroxiredoxin 6 Is a Key Antioxidant Enzyme in Modulating the Link between Glycemic and Lipogenic Metabolism","volume":"2019","author":"Arriga","year":"2019","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"753","DOI":"10.3892\/mmr.2015.4587","article-title":"Peroxiredoxin 6 attenuates ischemia- and hypoxia-induced liver damage of brain-dead donors","volume":"13","author":"Tu","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1089\/ars.2018.7544","article-title":"Peroxiredoxin 6 Confers Protection Against Nonalcoholic Fatty Liver Disease Through Maintaining Mitochondrial Function","volume":"31","author":"Lee","year":"2019","journal-title":"Antioxid. Redox Signal."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0167-4889(02)00264-1","article-title":"Molecular chaperones as essential mediators of mitochondrial biogenesis","volume":"1592","author":"Voos","year":"2002","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Renner, S.W., Walker, L.M., Forsberg, L.J., Sexton, J.Z., and Brenman, J.E. (2017). Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0176502"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1080\/1521-6540400000850","article-title":"Anti-Oxidative Response of Carbonic Anhydrase III in Skeletal Muscle","volume":"56","author":"Zimmerman","year":"2004","journal-title":"IUBMB Life"},{"key":"ref_82","first-page":"956.958","article-title":"Carbonic anhydrase mediates hepatic steatosis and vascular stiffness induced by a Western diet","volume":"30","author":"Malcolm","year":"2016","journal-title":"FASEB J. Off. Publ. Fed. of Am. Soc. Exp. Biol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1016\/j.aohep.2019.07.012","article-title":"Can Prohibitin 1 be a Safeguard against liver disease?","volume":"18","author":"Xia","year":"2019","journal-title":"Ann. Hepatol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1007\/s10495-015-1143-z","article-title":"Multifaceted role of prohibitin in cell survival and apoptosis","volume":"20","author":"Peng","year":"2015","journal-title":"Apoptosis"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1002\/hep.23919","article-title":"Liver-specific deletion of prohibitin 1 results in spontaneous liver injury, fibrosis, and hepatocellular carcinoma in mice","volume":"52","author":"Ko","year":"2010","journal-title":"Hepatology"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/bbrc.2000.3359","article-title":"The role of regucalcin in nuclear regulation of regenerating liver","volume":"276","author":"Yamaguchi","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"7","DOI":"10.15761\/IMM.1000102","article-title":"Regucalcin, a novel regulatory protein implicated in obesity and diabetes","volume":"1","author":"Yamaguchi","year":"2014","journal-title":"Integr. Mol. Med."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1023\/A:1020822610085","article-title":"Potential role of regucalcin as a specific biochemical marker of chronic liver injury with carbon tetrachloride administration in rats","volume":"241","author":"Yamaguchi","year":"2002","journal-title":"Mol. Cell Biochem."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1002\/hep.27068","article-title":"High-fat diet triggers Mallory-Denk body formation through misfolding and crosslinking of excess keratin 8","volume":"60","author":"Kucukoglu","year":"2014","journal-title":"Hepatology"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1002\/hep.25664","article-title":"Oxidative stress, Nrf2 and keratin up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria","volume":"56","author":"Singla","year":"2012","journal-title":"Hepatology"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1111\/liv.12608","article-title":"Keratins 8 and 18 are type II acute-phase responsive genes overexpressed in human liver disease","volume":"35","author":"Guldiken","year":"2015","journal-title":"Liver Int."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/hep.21471","article-title":"Keratin 18 overexpression but not phosphorylation or filament organization blocks mouse Mallory body formation","volume":"45","author":"Harada","year":"2007","journal-title":"Hepatology"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"73309","DOI":"10.18632\/oncotarget.12325","article-title":"Keratin 18-deficiency results in steatohepatitis and liver tumors in old mice: A model of steatohepatitis-associated liver carcinogenesis","volume":"7","author":"Bettermann","year":"2016","journal-title":"Oncotarget"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1002\/lt.23813","article-title":"Heat shock protein 70 is required for optimal liver regeneration after partial hepatectomy in mice","volume":"20","author":"Wolf","year":"2014","journal-title":"Liver Transpl."},{"key":"ref_95","first-page":"344","article-title":"Liver proteome changes induced by a short-term high-fat sucrose diet in wistar rats","volume":"4","author":"Paternain","year":"2011","journal-title":"J. Nutrigenet Nutrigenomics"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.tibs.2020.02.001","article-title":"Outlining the Complex Pathway of Mammalian Fe-S Cluster Biogenesis","volume":"45","author":"Maio","year":"2020","journal-title":"Trends Biochem. Sci."},{"key":"ref_97","first-page":"2161","article-title":"Characterization and prognostic significance of mortalin, Bcl-2 and Bax in intrahepatic cholangiocarcinoma","volume":"15","author":"Kang","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1074\/mcp.M700116-MCP200","article-title":"Association of Mortalin (HSPA9) with Liver Cancer Metastasis and Prediction for Early Tumor Recurrence","volume":"7","author":"Yi","year":"2008","journal-title":"Mol. Cell Proteomics"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/5\/1678\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:01:49Z","timestamp":1760162509000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/5\/1678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,15]]},"references-count":98,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["nu13051678"],"URL":"https:\/\/doi.org\/10.3390\/nu13051678","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,15]]}}}