{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:38:41Z","timestamp":1780706321464,"version":"3.54.1"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T00:00:00Z","timestamp":1621036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Introduction: The study of intraepithelial lymphocytes (IEL) by flow cytometry is a useful tool in the diagnosis of coeliac disease (CD). Previous data showed that an increase in %TCR\u03b3\u03b4+ and decrease of %CD3\u2212 IEL constitute a typical CD cytometric pattern with a specificity of 100%. However, there are no data regarding whether there are differences in the %TCR\u03b3\u03b4+ related to sex, age, titers of serology, and degree of histological lesion. Study aims: To confirm the high diagnostic accuracy of the coeliac cytometric patterns. To determine if there are differences between sex, age, serology titers, and histological lesion grade. Results: We selected all patients who fulfilled \u201c4 of 5\u201d rule for CD diagnosis (n = 169). There were no differences in %TCR\u03b3\u03b4+ between sexes (p = 0.909), age groups (p = 0.986), serology titers (p = 0.53) and histological lesion grades (p = 0.41). The diagnostic accuracy of complete CD cytometric pattern was: specificity 100%, sensitivity 82%, PPV 100%, NPV 47%. Conclusion: We confirmed, in a validation cohort, the high diagnostic accuracy of complete CD pattern irrespective of sex, age, serology titers, and grade of mucosal lesion.<\/jats:p>","DOI":"10.3390\/nu13051684","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T02:31:34Z","timestamp":1621218694000},"page":"1684","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Intraepithelial Lymphocyte Cytometric Pattern Is a Useful Diagnostic Tool for Coeliac Disease Diagnosis Irrespective of Degree of Mucosal Damage and Age\u2014A Validation Cohort"],"prefix":"10.3390","volume":"13","author":[{"given":"Pablo","family":"Ruiz-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Carreras","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Fajardo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Trist\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Salvador","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4023-8868","authenticated-orcid":false,"given":"Yamile","family":"Zabana","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"And\u00fajar","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carme","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Horta","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carme","family":"Loras","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Garc\u00eda-Puig","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1489-504X","authenticated-orcid":false,"given":"Fernando","family":"Fern\u00e1ndez-Ba\u00f1ares","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Esteve","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Universitari M\u00fatua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.amjmed.2010.02.019","article-title":"Celiac disease diagnosis: Simple rules are better than complicated algorithms","volume":"123","author":"Catassi","year":"2010","journal-title":"Am. 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