{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:04:07Z","timestamp":1790528647231,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:00:00Z","timestamp":1624924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"TargEDys SA","award":["TargEDys SA"],"award-info":[{"award-number":["TargEDys SA"]}]},{"name":"European Regional Development Fund (ERDF)","award":["European Regional Development Fund (ERDF)"],"award-info":[{"award-number":["European Regional Development Fund (ERDF)"]}]},{"name":"Ministry of Industry and Technology","award":["Ministry of Industry and Technology"],"award-info":[{"award-number":["Ministry of Industry and Technology"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>CLPB (Caseinolytic peptidase B) protein is a conformational mimetic of \u03b1-MSH, an anorectic hormone. Previous in vivo studies have already shown the potential effect of CLPB protein on food intake and on the production of peptide YY (PYY) by injection of E. coli wild type (WT) or E. coli \u0394ClpB. However, until now, no study has shown its direct effect on food intake. Furthermore, this protein can fragment naturally. Therefore, the aim of this study was (i) to evaluate the in vitro effects of CLPB fragments on PYY production; and (ii) to test the in vivo effects of a CLPB fragment sharing molecular mimicry with \u03b1-MSH (CLPB25) compared to natural fragments of the CLPB protein (CLPB96). To do that, a primary culture of intestinal mucosal cells from male Sprague\u2013Dawley rats was incubated with proteins extracted from E. coli WT and \u0394CLPB after fragmentation with trypsin or after a heat treatment of the CLPB protein. PYY secretion was measured by ELISA. CLPB fragments were analyzed by Western Blot using anti-\u03b1-MSH antibodies. In vivo effects of the CLPB protein on food intake were evaluated by intraperitoneal injections in male C57Bl\/6 and ob\/ob mice using the BioDAQ\u00ae system. The natural CLPB96 fragmentation increased PYY production in vitro and significantly decreased cumulative food intake from 2 h in C57Bl\/6 and ob\/ob mice on the contrary to CLPB25. Therefore, the anorexigenic effect of CLPB is likely the consequence of enhanced PYY secretion.<\/jats:p>","DOI":"10.3390\/nu13072223","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T04:31:07Z","timestamp":1624941067000},"page":"2223","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Effects of Bacterial CLPB Protein Fragments on Food Intake and PYY Secretion"],"prefix":"10.3390","volume":"13","author":[{"given":"Manon","family":"Dominique","sequence":"first","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"},{"name":"Inserm UMR1073, Nutrition, Gut and Brain Laboratory, University of Rouen Normandy, 76183 Rouen, France"},{"name":"Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Lucas","sequence":"additional","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-0537","authenticated-orcid":false,"given":"Romain","family":"Legrand","sequence":"additional","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Illona-Marie","family":"Boulet\u00e9","sequence":"additional","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"B\u00f4le-Feysot","sequence":"additional","affiliation":[{"name":"Inserm UMR1073, Nutrition, Gut and Brain Laboratory, University of Rouen Normandy, 76183 Rouen, France"},{"name":"Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Deroissart","sequence":"additional","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"L\u00e9on","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France"},{"name":"Animal Behavior Platform, Service Commun d\u2019Analyse Comportementale (SCAC), University of Rouen Normandy, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Nobis","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France"},{"name":"Animal Behavior Platform, Service Commun d\u2019Analyse Comportementale (SCAC), University of Rouen Normandy, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Claude","family":"do Rego","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France"},{"name":"Animal Behavior Platform, Service Commun d\u2019Analyse Comportementale (SCAC), University of Rouen Normandy, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3840-3935","authenticated-orcid":false,"given":"Gr\u00e9gory","family":"Lambert","sequence":"additional","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5224-6166","authenticated-orcid":false,"given":"Pierre","family":"D\u00e9chelotte","sequence":"additional","affiliation":[{"name":"TargEDys SA, 76183 Rouen, France"},{"name":"Inserm UMR1073, Nutrition, Gut and Brain Laboratory, University of Rouen Normandy, 76183 Rouen, France"},{"name":"Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen Normandy, 76183 Rouen, France"},{"name":"Rouen University Hospital, CHU Charles Nicolle, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2019, February 16). 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