{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:59:28Z","timestamp":1787029168991,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T00:00:00Z","timestamp":1626480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Anxiety appears among the most frequent psychiatric disorders. During recent years, a growing incidence of anxiety disorders can be attributed, at least in part, to the modification of our eating habits. To treat anxiety disorders, clinicians use benzodiazepines, which unfortunately display many side effects. Herein, the anxiolytic-like properties of two natural products (\u03b1S1\u2013casein hydrolysate and Gabolysat\u00ae) were investigated in rats and compared to the efficacy of benzodiazepine (diazepam). Thus, the conditioned defensive burying test was performed after a unique oral dose of 15 mg\/kg, at two time-points (60 min and then 30 min post oral gavage) to show potential fast-onset of anxiolytic effect. Both natural products proved to be as efficient as diazepam to reduce the time rats spent burying the probe (anxiety level). Additionally, when investigated as early as 30 min post oral gavage, Gabolysat\u00ae also revealed a fast-anxiolytic activity. To date, identification of bioactive peptide, as well as how they interact with the gut\u2013brain axis to sustain such anxiolytic effect, still remains poorly understood. Regardless, this observational investigation argues for the consideration of natural compounds in care pathway.<\/jats:p>","DOI":"10.3390\/nu13072445","type":"journal-article","created":{"date-parts":[[2021,7,18]],"date-time":"2021-07-18T21:18:52Z","timestamp":1626643132000},"page":"2445","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Fast Anxiolytic-Like Effect Observed in the Rat Conditioned Defensive Burying Test, after a Single Oral Dose of Natural Protein Extract Products"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4446-4451","authenticated-orcid":false,"given":"Thomas","family":"Freret","sequence":"first","affiliation":[{"name":"UNICAEN, INSERM, COMETE, Cyceron, CHU Caen, Normandie University, 14000 Caen, France"},{"name":"Behavioral Research Platform, Normandie University, 14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy","family":"Largilliere","sequence":"additional","affiliation":[{"name":"UNICAEN, INSERM, COMETE, Cyceron, CHU Caen, Normandie University, 14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Nee","sequence":"additional","affiliation":[{"name":"UNICAEN, INSERM, COMETE, Cyceron, CHU Caen, Normandie University, 14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Coolzaet","sequence":"additional","affiliation":[{"name":"UNICAEN, INSERM, COMETE, Cyceron, CHU Caen, Normandie University, 14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Corvaisier","sequence":"additional","affiliation":[{"name":"UNICAEN, INSERM, COMETE, Cyceron, CHU Caen, Normandie University, 14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Boulouard","sequence":"additional","affiliation":[{"name":"UNICAEN, INSERM, COMETE, Cyceron, CHU Caen, Normandie University, 14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1517\/14728214.2015.1049996","article-title":"Emerging drugs for the treatment of anxiety","volume":"20","author":"Murrough","year":"2015","journal-title":"Expert Opin. 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