{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:09:33Z","timestamp":1791504573903,"version":"4.3.4"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T00:00:00Z","timestamp":1627430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["402343\/2012-3; 401605\/2013\u20102; 422441\/2016-3, 306525\/2019-4, 310578\/2020-5"],"award-info":[{"award-number":["402343\/2012-3; 401605\/2013\u20102; 422441\/2016-3, 306525\/2019-4, 310578\/2020-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E26\/201.190\/2015"],"award-info":[{"award-number":["E26\/201.190\/2015"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Assisted reproductive technologies (ART) may increase risk for abnormal placental development, preterm delivery and low birthweight. We investigated placental morphology, transporter expression and paired maternal\/umbilical fasting blood nutrient levels in human term pregnancies conceived naturally (n = 10) or by intracytoplasmic sperm injection (ICSI; n = 11). Maternal and umbilical vein blood from singleton term (&gt;37 weeks) C-section pregnancies were assessed for levels of free amino acids, glucose, free fatty acids (FFA), cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low-density lipoprotein (VLDL) and triglycerides. We quantified placental expression of GLUT1 (glucose), SNAT2 (amino acids), P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) (drug) transporters, and placental morphology and pathology. Following ICSI, placental SNAT2 protein expression was downregulated and umbilical cord blood levels of citrulline were increased, while FFA levels were decreased at term (p &lt; 0.05). Placental proliferation and apoptotic rates were increased in ICSI placentae (p &lt; 0.05). No changes in maternal blood nutrient levels, placental GLUT1, P-gp and BCRP expression, or placental histopathology were observed. In term pregnancies, ICSI impairs placental SNAT2 transporter expression and cell turnover, and alters umbilical vein levels of specific nutrients without changing placental morphology. These may represent mechanisms through which ICSI impacts pregnancy outcomes and programs disease risk trajectories in offspring across the life course.<\/jats:p>","DOI":"10.3390\/nu13082587","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T05:23:48Z","timestamp":1627449828000},"page":"2587","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Altered Umbilical Cord Blood Nutrient Levels, Placental Cell Turnover and Transporter Expression in Human Term Pregnancies Conceived by Intracytoplasmic Sperm Injection (ICSI)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0275-8686","authenticated-orcid":false,"given":"Enrrico","family":"Bloise","sequence":"first","affiliation":[{"name":"Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-910, Brazil"},{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jair R. S.","family":"Braga","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"},{"name":"Maternidade Escola, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 22240-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4320-4252","authenticated-orcid":false,"given":"Cherley B. V.","family":"Andrade","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guinever E.","family":"Imperio","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"},{"name":"Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5G 1X5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian M.","family":"Martinelli","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-910, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6544-054X","authenticated-orcid":false,"given":"Roberto A.","family":"Antunes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"},{"name":"Maternidade Escola, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 22240-000, Brazil"},{"name":"Fertipraxis-Centro de Reprodu\u00e7\u00e3o Humana, Rio de Janeiro, RJ 22640-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7394-2494","authenticated-orcid":false,"given":"Karina R.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Molecular, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5266-6870","authenticated-orcid":false,"given":"Cristiana B.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Departamento de Anatomia Patol\u00f3gica e Medicina Legal, Universidade Federal de Minas Gerais, Belo Horizonte, MG 30130-100, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Cobellis","sequence":"additional","affiliation":[{"name":"Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d, 80138 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5207-2896","authenticated-orcid":false,"given":"Flavia F.","family":"Bloise","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen G.","family":"Matthews","sequence":"additional","affiliation":[{"name":"Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5G 1X5, Canada"},{"name":"Department of Obstetrics and Gynaecology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 3H2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin L.","family":"Connor","sequence":"additional","affiliation":[{"name":"Health Sciences, Carleton University, Ottawa, ON K1S 5B6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0225-1132","authenticated-orcid":false,"given":"Tania M.","family":"Ortiga-Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Endocrinologia Translacional, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1093\/humrep\/dew082","article-title":"International Committee for Monitoring Assisted Reproductive Technologies world report: Assisted Reproductive Technology 2008, 2009 and 2010","volume":"31","author":"Dyer","year":"2016","journal-title":"Hum. 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