{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T20:18:26Z","timestamp":1785356306617,"version":"3.55.0"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Cyclophosphamide (CP)\u2014which is used to treat autoimmune diseases and cancer\u2014is related to gonadotoxicity attributed to oxidative stress. As phycobiliproteins (PBPs) are strong antioxidants that are unexplored as protective agents against male gonadotoxicity, our work aimed to investigate the effects of PBP crude extract on testicular damage and sperm parameter alterations caused by CP in mice. Three doses of PBP (50, 100, and 200 mg\/kg) were tested in the experimental groups (n = 8 per group), administered concomitantly with 100 mg\/kg CP. After 42 days receiving PBP daily and CP weekly, body and relative testicular weights, serum testosterone levels, testicular lipoperoxidation and antioxidant enzyme activity levels, and testicular histology and sperm parameter alterations were assessed. The results showed that PBP crude extract at 200 mg\/kg prevented testosterone serum reduction, body weight loss, lipoperoxidation and enzyme activity increments, and sperm parameter alterations and partially ameliorated relative testicular weight reductions and histological damage in CP-treated mice. In conclusion, we showed that PBP crude extract (200 mg\/kg) mitigated oxidative damage in the testes and ameliorated alterations in sperm parameters in mice treated with CP (100 mg\/kg); therefore, PBP extract could be considered as a potential protective agent against CP toxicity.<\/jats:p>","DOI":"10.3390\/nu13082616","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T21:21:21Z","timestamp":1627593681000},"page":"2616","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide"],"prefix":"10.3390","volume":"13","author":[{"given":"Jorge","family":"Brise\u00f1o-Bugar\u00edn","sequence":"first","affiliation":[{"name":"Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Unidad Zacatenco, Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Hern\u00e1ndez-Ochoa","sequence":"additional","affiliation":[{"name":"Departamento de Toxicolog\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional (Cinvestav-IPN), Unidad Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1410-7207","authenticated-orcid":false,"given":"Xelha","family":"Araujo-Padilla","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Unidad Zacatenco, Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1881-5374","authenticated-orcid":false,"given":"Mar\u00eda Ang\u00e9lica","family":"Mojica-Villegas","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Unidad Zacatenco, Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7729-4092","authenticated-orcid":false,"given":"Ricardo Iv\u00e1n","family":"Monta\u00f1o-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Unidad Zacatenco, Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3651-0865","authenticated-orcid":false,"given":"Gabriela","family":"Guti\u00e9rrez-Salme\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud, Universidad An\u00e1huac M\u00e9xico, Mexico City 52786, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8935-9831","authenticated-orcid":false,"given":"Germ\u00e1n","family":"Chamorro-Cevallos","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Unidad Zacatenco, Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3724\/SP.J.1009.2012.00247","article-title":"Achillea millefolium inflorescence aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: Stereological evidences","volume":"10","author":"Jalali","year":"2012","journal-title":"Chin. 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