{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T23:50:40Z","timestamp":1783381840668,"version":"3.54.6"},"reference-count":72,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T00:00:00Z","timestamp":1632441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016308","name":"Bpifrance","doi-asserted-by":"publisher","award":["not available"],"award-info":[{"award-number":["not available"]}],"id":[{"id":"10.13039\/100016308","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background. Carotenoids and docosahexaenoic acid (DHA) were identified as essential components for eye health and are both naturally present in eggs. Objective. We aimed to evaluate the effect of the daily consumption of two eggs enriched with lutein\/zeaxanthin and DHA on macular pigment optical density (MPOD) and on circulating xanthophyll and fatty acid concentrations in healthy participants. Methods. Ninety-nine healthy volunteers consumed either two standard eggs or two enriched eggs per day for 4 months. MPOD was measured at baseline (V0) and at follow-up (V4) using a modified confocal scanning laser ophthalmoscope (primary outcome). Blood samples were collected to determine total plasma and lipoprotein fatty acids and lutein\/zeaxanthin compositions at V0 and V4 (secondary outcomes). Results. A slight but significant increase in MPOD was observed for all study participants consuming two eggs per day for 4 months at all eccentricities (0.5\u00b0, 1\u00b0, 2\u00b0, and 4\u00b0). Plasma and lipoprotein lutein, zeaxanthin, and DHA concentrations significantly increased in both groups but were greater in the enriched group (for the enriched group (V0 vs. V4): lutein, 167 vs. 369 ng\/mL; zeaxanthin, 17.7 vs. 29.2 ng\/mL; DHA, 1.89 vs. 2.56% of total fatty acids). Interestingly, lutein from high-density lipoprotein (HDL) was strongly correlated with MPOD at 0.5 and 1\u00b0 eccentricities (rho = 0.385, p = 0.008, and rho = 0.461, p = 0.001, respectively). Conclusions. MPOD was slightly increased in both groups. Lutein, zeaxanthin, and DHA plasma concentrations were strongly enhanced in the enriched group compared with the standard group. A significant correlation was found between MPOD level and lutein concentration in HDL.<\/jats:p>","DOI":"10.3390\/nu13103347","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T21:40:30Z","timestamp":1632778830000},"page":"3347","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The ALGOVUE Clinical Trial: Effects of the Daily Consumption of Eggs Enriched with Lutein and Docosahexaenoic Acid on Plasma Composition and Macular Pigment Optical Density"],"prefix":"10.3390","volume":"13","author":[{"given":"Coralie","family":"Schnebelen-Berthier","sequence":"first","affiliation":[{"name":"Service Nutrition & Activit\u00e9 Physique, Institut Pasteur de Lille, F-59019 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niyazi","family":"Acar","sequence":"additional","affiliation":[{"name":"Centre des Sciences du Go\u00fbt et de l\u2019Alimentation, Agrosup Dijon, CNRS, INRA, Universit\u00e9 Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Simon","sequence":"additional","affiliation":[{"name":"Service Nutrition & Activit\u00e9 Physique, Institut Pasteur de Lille, F-59019 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mentine","family":"Thabuis","sequence":"additional","affiliation":[{"name":"ROQUETTE, F-62080 Lestrem, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bourdillon","sequence":"additional","affiliation":[{"name":"MIXSCIENCE, Centre d\u2019Affaires Odyss\u00e9e, ZAC Cic\u00e9 Blossac, F-35172 Bruz, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Mathiaud","sequence":"additional","affiliation":[{"name":"MIXSCIENCE, Centre d\u2019Affaires Odyss\u00e9e, ZAC Cic\u00e9 Blossac, F-35172 Bruz, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2398-0596","authenticated-orcid":false,"given":"Luc","family":"Dauchet","sequence":"additional","affiliation":[{"name":"CNRS, INSERM, CHU de Lille, Institut Pasteur de Lille, UMR 1283-EGID, \u201cIntegrative Genomics and Modelling of Metabolic Diseases\u201d, Universit\u00e9 de Lille, F-59045 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2099-0481","authenticated-orcid":false,"given":"C\u00e9cile","family":"Delcourt","sequence":"additional","affiliation":[{"name":"\u201cBordeaux Population Health Research Center\u201d (BPH), INSERM, UMR1219, \u201cLifelong Exposures, Health and Ageing\u201d (LEHA) Group, Universit\u00e9 de Bordeaux, F-33076 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Benlian","sequence":"additional","affiliation":[{"name":"CNRS, INSERM, CHU de Lille, Institut Pasteur de Lille, UMR 1283-EGID, \u201cIntegrative Genomics and Modelling of Metabolic Diseases\u201d, Universit\u00e9 de Lille, F-59045 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Crochet","sequence":"additional","affiliation":[{"name":"CHU Lille, Service d\u2019Exploration de la Vision et Neuro-Ophtalmologie, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Defoort","sequence":"additional","affiliation":[{"name":"CHU Lille, Service d\u2019Exploration de la Vision et Neuro-Ophtalmologie, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tailleux","sequence":"additional","affiliation":[{"name":"CNRS, INSERM, CHU de Lille, Institut Pasteur de Lille, UMR 1283-EGID, \u201cIntegrative Genomics and Modelling of Metabolic Diseases\u201d, Universit\u00e9 de Lille, F-59045 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3784-1503","authenticated-orcid":false,"given":"Bart","family":"Staels","sequence":"additional","affiliation":[{"name":"CNRS, INSERM, CHU de Lille, Institut Pasteur de Lille, UMR 1283-EGID, \u201cIntegrative Genomics and Modelling of Metabolic Diseases\u201d, Universit\u00e9 de Lille, F-59045 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6957-100X","authenticated-orcid":false,"given":"Lionel","family":"Bretillon","sequence":"additional","affiliation":[{"name":"Centre des Sciences du Go\u00fbt et de l\u2019Alimentation, Agrosup Dijon, CNRS, INRA, Universit\u00e9 Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Lecerf","sequence":"additional","affiliation":[{"name":"Service Nutrition & Activit\u00e9 Physique, Institut Pasteur de Lille, F-59019 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1016\/S0140-6736(12)60282-7","article-title":"Age-related macular degeneration","volume":"379","author":"Lim","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.ophtha.2007.03.083","article-title":"Dietary antioxidants and the long-term incidence of age-related macular degeneration","volume":"115","author":"Tan","year":"2008","journal-title":"Ophthalmology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1167\/iovs.05-1235","article-title":"Plasma lutein and zeaxanthin and other carotenoids as modifiable risk factors for age-related maculopathy and cataract: The POLA study","volume":"47","author":"Delcourt","year":"2006","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1038\/sj.ejcn.1602685","article-title":"Dietary fat and the risk of age-related maculopathy: The POLANUT study","volume":"61","author":"Delcourt","year":"2007","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1001\/archopht.124.8.1151","article-title":"Associations between intermediate age-related macular degeneration and lutein and zeaxanthin in the carotenoids in age-related eye disease study (CAREDS): Ancillary study of the women\u2019s health Initiative","volume":"124","author":"Moeller","year":"2006","journal-title":"Arch. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1093\/ajcn\/88.2.398","article-title":"Oily fish consumption, dietary docosahexaenoic acid and eicosapentaenoic acid intakes, and associations with neovascular age-related macular degeneration","volume":"88","author":"Augood","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.3945\/ajcn.2009.27594","article-title":"Omega-3 long-chain polyunsaturated fatty acid intake and 12-y incidence of neovascular age-related macular degeneration and central geographic atrophy: AREDS report 30, a prospective cohort study from the age-related eye disease study","volume":"90","author":"SanGiovanni","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1001\/archopht.124.7.1038","article-title":"Macular pigment: A review of current knowledge","volume":"124","author":"Whitehead","year":"2006","journal-title":"Arch. Ophthalmol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3934","DOI":"10.1167\/iovs.10-5898","article-title":"Nutritional manipulation of primate retinas, V: Effects of Lutein, Zeaxanthin, and n-3 fatty acids on retinal sensitivity to blue-light-induced damage","volume":"52","author":"Barker","year":"2011","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1006\/abbi.2001.2411","article-title":"Macular pigments lutein and zeaxanthin as blue light filters studied in liposomes","volume":"391","author":"Junghans","year":"2001","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.preteyeres.2004.05.004","article-title":"Macular pigments: Their characteristics and putative role","volume":"23","author":"Davies","year":"2004","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0163-7827(83)90004-8","article-title":"Chemistry and metabolism of lipids in the vertebrate retina","volume":"22","author":"Fliesler","year":"1983","journal-title":"Prog. Lipid Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"S193","DOI":"10.1007\/BF02637075","article-title":"A role for phospholipid polyunsaturation in modulating membrane protein function","volume":"31","author":"Litman","year":"1996","journal-title":"Lipids"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4866","DOI":"10.1167\/iovs.07-0918","article-title":"Homeostatic regulation of photoreceptor cell integrity: Significance of the potent mediator neuroprotectin D1 biosynthesized from docosahexaenoic acid the proctor lecture","volume":"48","author":"Bazan","year":"2007","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.1093\/jn\/119.12.1880","article-title":"The effects of dietary alpha-linolenic acid on the composition of nerve membranes, enzymatic activity, amplitude of electrophysiological parameters, resistance to poisons and performance of learning tasks in rats","volume":"119","author":"Bourre","year":"1989","journal-title":"J. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BF02522413","article-title":"The effect of docosahexaenoic acid on the electroretinogram of the guinea pig","volume":"31","author":"Weisinger","year":"1996","journal-title":"Lipids"},{"key":"ref_17","first-page":"2243","article-title":"Retinal light damage in rats with altered levels of rod outer segment docosahexaenoate","volume":"37","author":"Organisciak","year":"1996","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1093\/ajcn\/85.3.762","article-title":"Effect of dietary lutein and zeaxanthin on plasma carotenoids and their transport in lipoproteins in age-related macular degeneration","volume":"85","author":"Wang","year":"2007","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.preteyeres.2012.03.002","article-title":"Lutein: More than just a filter for blue light","volume":"31","author":"Kijlstra","year":"2012","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"232s","DOI":"10.1093\/ajcn\/71.1.232S","article-title":"Effect of long-chain n-3 polyunsaturated fatty acids on fasting and postprandial triacylglycerol metabolism","volume":"71","author":"Roche","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.plefa.2009.05.016","article-title":"Docosahexaenoic acid (DHA) and cardiovascular disease risk factors","volume":"81","author":"Holub","year":"2009","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1093\/ajcn\/87.5.1521","article-title":"The influence of supplemental lutein and docosahexaenoic acid on serum, lipoproteins, and macular pigmentation","volume":"87","author":"Johnson","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"543","DOI":"10.3390\/nu5020543","article-title":"Effects of lutein and docosahexaenoic acid supplementation on macular pigment optical density in a randomized controlled trial","volume":"5","author":"Recalde","year":"2013","journal-title":"Nutrients"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1007\/s00417-013-2376-6","article-title":"Long term effects of lutein, zeaxanthin and omega-3-LCPUFAs supplementation on optical density of macular pigment in AMD patients: The LUTEGA study","volume":"251","author":"Dawczynski","year":"2013","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plefa.2013.03.010","article-title":"Bioavailability of long-chain omega-3 fatty acids","volume":"89","author":"Schuchardt","year":"2013","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.cnd.2010.04.004","article-title":"Egg fatty acids composition. Nutritional interest and health value","volume":"45","author":"Pieroni","year":"2010","journal-title":"Cah. Nutr. Di\u00e9t\u00e9tique"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1051\/mnut\/2005413116","article-title":"L\u2019\u0153uf naturel multi-enrichi: Des apports \u00e9lev\u00e9s en nutriments, notamment acides gras om\u00e9ga-3, en vitamines, min\u00e9raux et carot\u00e9no\u00efdes","volume":"41","author":"Bourre","year":"2005","journal-title":"Med. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1172\/JCI103182","article-title":"The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum","volume":"34","author":"Havel","year":"1955","journal-title":"J. Clin. Investig."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0009-8981(81)90235-7","article-title":"The effect of storage on the fatty acid composition of human serum","volume":"114","author":"Moilanen","year":"1981","journal-title":"Clin. Chim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/S0022-2275(20)40190-7","article-title":"Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride\u2014Methanol","volume":"5","author":"Morrison","year":"1964","journal-title":"J. Lipid Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0378-4347(00)00488-6","article-title":"Simple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method","volume":"751","author":"Lyan","year":"2001","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3976","DOI":"10.1167\/iovs.07-0208","article-title":"Modified saponification and HPLC methods for analyzing carotenoids from the retina of quail: Implications for its use as a nonprimate model species","volume":"48","author":"Toomey","year":"2007","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/s00417-003-0730-9","article-title":"Macular pigment density in healthy subjects quantified with a modified confocal scanning laser ophthalmoscope","volume":"241","author":"Moessner","year":"2003","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1007\/s00417-002-0515-6","article-title":"A new instrument for the quantification of macular pigment density: First results in patients with AMD and healthy subjects","volume":"240","author":"Jahn","year":"2002","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.3945\/ajcn.2009.28013","article-title":"Consumption of 2 and 4 egg yolks\/d for 5 wk increases macular pigment concentrations in older adults with low macular pigment taking cholesterol-lowering statins","volume":"90","author":"Vishwanathan","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1364\/JOSAA.14.001187","article-title":"Individual variations in the spatial profile of human macular pigment","volume":"14","author":"Hammond","year":"1997","journal-title":"J. Opt. Soc. Am. A"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1167\/iovs.05-0663","article-title":"Macular pigment shows ringlike structures","volume":"47","author":"Berendschot","year":"2006","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1007\/s00394-012-0447-9","article-title":"Effects of formulation on the bioavailability of lutein and zeaxanthin: A randomized, double-blind, cross-over, comparative, single-dose study in healthy subjects","volume":"52","author":"Evans","year":"2013","journal-title":"Eur. J. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1017\/S0954422407842235","article-title":"Macular zeaxanthins and lutein\u2014A review of dietary sources and bioavailability and some relationships with macular pigment optical density and age-related macular disease","volume":"20","author":"Thurnham","year":"2007","journal-title":"Nutr. Res. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1016\/j.ophtha.2013.01.005","article-title":"Oral docosahexaenoic acid in the prevention of exudative age-related macular degeneration: The nutritional AMDtreatment 2 study","volume":"120","author":"Souied","year":"2013","journal-title":"Ophthalmology"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Age-Related Eye Disease Study 2 Research Group (2013). Lutein + Zeaxanthin and Omega-3 fatty acids for age-related macular degeneration. JAMA, 309, 2005\u20132015.","DOI":"10.1001\/jama.2013.4997"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1039\/C2FO30154G","article-title":"Egg intake improves carotenoid status by increasing plasma HDL cholesterol in adults with metabolic syndrome","volume":"4","author":"Blesso","year":"2013","journal-title":"Food Funct."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1194\/jlr.M070193","article-title":"Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins","volume":"57","author":"Thomas","year":"2016","journal-title":"J. Lipid Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1017\/S0007114516003895","article-title":"Serum and macular response to carotenoid-enriched egg supplementation in human subjects: The egg xanthophyll intervention clinical trial (EXIT)","volume":"117","author":"Kelly","year":"2017","journal-title":"Br. J. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Obana, A., Tanito, M., Gohto, Y., Okazaki, S., Gellermann, W., and Bernstein, P.S. (2015). Changes in macular pigment optical density and serum lutein concentration in Japanese subjects taking two different lutein supplements. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0139257"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Bone, R.A., Davey, P.G., Roman, B.O., and Evans, D.W. (2020). Efficacy of commercially available nutritional supplements: Analysis of serum uptake, macular pigment optical density and visual functional response. Nutrients, 12.","DOI":"10.3390\/nu12051321"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1093\/jn\/134.8.1887","article-title":"Human nutrition and metabolism lutein bioavailability is higher from lutein-enriched eggs than from supplements and spinach","volume":"134","author":"Chung","year":"2004","journal-title":"J. Nutr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s12937-015-0089-x","article-title":"A pilot comparison of phospolipidated lutein to conventional lutein for effects on plasma lutein concentrations in adult people","volume":"14","author":"DiSilvestro","year":"2015","journal-title":"Nutr. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3945\/ajcn.115.111062","article-title":"Effects of egg consumption on carotenoid absorption from co-consumed, raw vegetables","volume":"102","author":"Kim","year":"2015","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_50","first-page":"61","article-title":"Pr\u00e9vention de la d\u00e9g\u00e9n\u00e9rescence maculaire li\u00e9e \u00e0 l\u2019\u00e2ge","volume":"29","author":"Couasnon","year":"2010","journal-title":"J. Pharm. Clin."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s11745-004-1259-6","article-title":"Dietary intakes and food sources of n-6 and n-3 PUFA in French adult men and women","volume":"39","author":"Astorg","year":"2004","journal-title":"Lipids"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1001\/jamaophthalmol.2017.3398","article-title":"Effect of dietary supplementation with Lutein, Zeaxanthin, and \u03c9-3 on macular pigment","volume":"135","author":"Korobelnik","year":"2017","journal-title":"JAMA Ophthalmol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1001\/jamaophthalmol.2013.7376","article-title":"Secondary analyses of the effects of lutein\/zeaxanthin on age-related macular degeneration progression: AREDS2 report no. 3","volume":"132","author":"Chew","year":"2014","journal-title":"JAMA Ophthalmol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.numecd.2013.06.009","article-title":"Lower dairy products and calcium intake is associated with adverse retinal vascular changes in older adults","volume":"24","author":"Gopinath","year":"2014","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.2147\/OPTH.S119251","article-title":"Preliminary analysis of the relationship between serum lutein and zeaxanthin levels and macular pigment optical density","volume":"10","author":"Fujimura","year":"2016","journal-title":"Clin. Ophthalmol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/1475-2891-13-52","article-title":"Markers of lutein and zeaxanthin status in two age groups of men and women: Dietary intake, serum concentrations, lipid profile and macular pigment optical density","volume":"13","year":"2014","journal-title":"Nutr. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.abb.2010.06.019","article-title":"Dose-dependent response of serum lutein and macular pigment optical density to supplementation with lutein esters","volume":"504","author":"Bone","year":"2010","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1167\/iovs.09-3396","article-title":"The relationship of macular pigment optical density to serum lutein in retinitis pigmentosa","volume":"51","author":"Sandberg","year":"2010","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"6178","DOI":"10.1167\/iovs.12-10275","article-title":"Macular pigment imaging in AREDS2 participants: An ancillary study of AREDS2 subjects enrolled at the moran eye center","volume":"53","author":"Bernstein","year":"2012","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_60","first-page":"1225","article-title":"Serum lipid fractions and risk of coronary heart disease. The Framingham study","volume":"52","author":"Kannel","year":"1969","journal-title":"Minn. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1017\/BJN20061937","article-title":"Egg consumption, serum total cholesterol concentrations and coronary heart disease incidence: Japan Public Health Center-based prospective study","volume":"96","author":"Nakamura","year":"2006","journal-title":"Br. J. Nutr."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.nut.2013.03.020","article-title":"The influence of consuming an egg or an egg-yolk buttermilk drink for 12 wk on serum lipids, inflammation, and liver function markers in human volunteers","volume":"29","author":"Baumgartner","year":"2013","journal-title":"Nutrition"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"315","DOI":"10.3109\/2000-1967-235","article-title":"Biochemical effects of consumption of eggs containing omega-3 polyunsaturated fatty acids","volume":"113","author":"Ohman","year":"2008","journal-title":"Upsala J. Med. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1080\/07315724.2016.1152928","article-title":"Meta-analysis of egg consumption and risk of coronary heart disease and stroke","volume":"35","author":"Alexander","year":"2016","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"966","DOI":"10.3945\/ajcn.111.029116","article-title":"Protein supplementation lowers blood pressure in overweight adults: Effect of dietary proteins on blood pressure (PROPRES), a randomized trial","volume":"95","author":"Dopheide","year":"2012","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.amjcard.2005.12.026","article-title":"Antiatherosclerotic and antithrombotic effects of omega-3 fatty acids","volume":"98","author":"Robinson","year":"2006","journal-title":"Am. J. Cardiol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Ma, L., Liu, R., Du, J., Liu, T., Wu, S., and Liu, X. (2016). Lutein, Zeaxanthin and meso-zeaxanthin supplementation associated with macular pigment optical density. Nutrients, 8.","DOI":"10.3390\/nu8070426"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1364\/JOSAA.18.001212","article-title":"Macular pigment density measured by autofluorescence spectrometry: Comparison with reflectometry and heterochromatic flicker photometry","volume":"18","author":"Delori","year":"2001","journal-title":"J. Opt. Soc. Am. A"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e763","DOI":"10.1111\/aos.13408","article-title":"Plasma long-chain omega-3 polyunsaturated fatty acids and macular pigment in subjects with family history of age-related macular degeneration: The Limpia study","volume":"95","author":"Merle","year":"2017","journal-title":"Acta Ophthalmol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.exer.2013.08.014","article-title":"Concordance of macular pigment measurements obtained using customized heterochromatic flicker photometry, dual-wavelength autofluorescence, and single-wavelength reflectance","volume":"116","author":"Dennison","year":"2013","journal-title":"Exp. Eye Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1167\/iovs.13-13568","article-title":"Comparison of two methods to measure macular pigment optical density in healthy subjects","volume":"55","author":"Koehrer","year":"2014","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.preteyeres.2015.10.003","article-title":"Lutein, zeaxanthin, and meso-zeaxanthin: The basic and clinical science underlying carotenoid-based nutritional interventions against ocular disease","volume":"50","author":"Bernstein","year":"2015","journal-title":"Prog. Retin. Eye Res."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/10\/3347\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:04:45Z","timestamp":1760166285000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/10\/3347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,24]]},"references-count":72,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["nu13103347"],"URL":"https:\/\/doi.org\/10.3390\/nu13103347","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,24]]}}}