{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T10:36:06Z","timestamp":1782383766257,"version":"3.54.5"},"reference-count":35,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T00:00:00Z","timestamp":1634083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001648","name":"European Foundation for the Study of Diabetes","doi-asserted-by":"publisher","award":["Lilly Programme, 2015"],"award-info":[{"award-number":["Lilly Programme, 2015"]}],"id":[{"id":"10.13039\/501100001648","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades, Spain","award":["SAF2017-84542-R"],"award-info":[{"award-number":["SAF2017-84542-R"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI14\/00035; CPII16\/00046"],"award-info":[{"award-number":["PI14\/00035; CPII16\/00046"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["N\/A; postdoctoral fellowships"],"award-info":[{"award-number":["N\/A; postdoctoral fellowships"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Background: Time restricted feeding (TRF) refers to dietary interventions in which food access is limited during a specific timeframe of the day. TRFs have proven useful in improving metabolic health in adult subjects with obesity. Their beneficial effects are mediated, in part, through modulating the circadian rhythm. Nevertheless, the translation of these dietary interventions onto obese\/overweight children and adolescents remains uncharacterized. The objective of this study is to explore the feasibility of temporal dietary interventions for improving metabolic health in the context of childhood obesity. Methods: We have previously developed a mouse model of early adiposity (i.e., childhood obesity) through litter size reduction. Mice raised in small litters (SL) became obese as early as by two weeks of age, and as adults, they developed several obesity-related co-morbidities, including insulin resistance, glucose intolerance and hepatic steatosis. Here, we explored whether two independent short-term chrono-nutritional interventions might improve metabolic health in 1-month-old pre-pubertal SL mice. Both TRFs comprised 8 h feeding\/14 h fasting. In the first one (TRF1) Control and SL mice had access to the diet for 8 h during the dark phase. In the second intervention (TRF2) food was available during the light:dark transitions. Results: TRF1 did not alter food intake nor ameliorate adiposity in SL-TRF1. In contrast, SL-TRF2 mice showed unintentional reduction of caloric intake, which was accompanied by reduced total body weight and adiposity. Strikingly, hepatic triglyceride content was completely normalized in SL-TRF1 and SL-TRF2 mice, when compared to the ad lib-fed SL mice. These effects were partially mediated by (i) clock-dependent signals, which might modulate the expression of Pparg or Cpt1a, and (ii) clock-independent signals, such as fasting itself, which could influence Fasn expression. Conclusions: Time-restricted feeding is an effective and feasible nutritional intervention to improve metabolic health, namely hepatic steatosis, in a model of childhood obesity. These data open new avenues for future safe and efficient chrono-nutritional interventions aimed to improve metabolic health in children with overweight\/obesity.<\/jats:p>","DOI":"10.3390\/nu13103579","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T21:48:39Z","timestamp":1634161719000},"page":"3579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Time-Restricted Feeding during Puberty Ameliorates Adiposity and Prevents Hepatic Steatosis in a Mouse Model of Childhood Obesity"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7672-4775","authenticated-orcid":false,"given":"Francesc","family":"Ribas-Aulinas","sequence":"first","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Esplugues, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0433-6081","authenticated-orcid":false,"given":"Marcela","family":"Parra-Vargas","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Esplugues, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Ramon-Krauel","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Esplugues, 08950 Barcelona, Spain"},{"name":"School of Medicine, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Diaz","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Esplugues, 08950 Barcelona, Spain"},{"name":"School of Medicine, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carles","family":"Lerin","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Esplugues, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9009-4690","authenticated-orcid":false,"given":"Trinitat","family":"Cambras","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Physiology, School of Pharmacy, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep C.","family":"Jimenez-Chillaron","sequence":"additional","affiliation":[{"name":"Institut de Recerca Sant Joan de D\u00e9u, Hospital Sant Joan de D\u00e9u, Esplugues, 08950 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2042","DOI":"10.1001\/jama.2010.1635","article-title":"Association of adolescent obesity with risk of severe obesity in adulthood","volume":"304","author":"The","year":"2010","journal-title":"JAMA J. Am. Med. Assoc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ijo.2011.186","article-title":"Childhood obesity and risk of the adult metabolic syndrome: A systematic review","volume":"36","author":"Lloyd","year":"2012","journal-title":"Int. J. Obes."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/clpt.2011.88","article-title":"Childhood obesity: A ticking time bomb for cardiovascular disease","volume":"90","author":"Logue","year":"2011","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_4","first-page":"174","article-title":"Long-term impact of overweight and obesity in childhood and adolescence on morbidity and premature mortality in adulthood: Systematic review","volume":"90","author":"Reilly","year":"2011","journal-title":"Int. J. Obes."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1210\/jc.2016-2573","article-title":"Pediatric obesity\u2014Assessment, treatment, and prevention: An endocrine society clinical practice guideline","volume":"102","author":"Styne","year":"2017","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.mayocp.2016.09.017","article-title":"Review of Childhood Obesity: From Epidemiology, Etiology, and Comorbidities to Clinical Assessment and Treatment","volume":"92","author":"Kumar","year":"2017","journal-title":"Mayo Clin. Proc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1136\/postgradmedj-2014-133033","article-title":"Severe childhood obesity: An under-recognised and growing health problem","volume":"91","author":"Bass","year":"2015","journal-title":"Postgrad. Med. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.earlhumdev.2006.07.004","article-title":"Long-term consequences of early nutrition","volume":"82","author":"Demmelmair","year":"2006","journal-title":"Early Hum. Dev."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Koletzko, B., Decsi, T., Moln\u00e1r, D., and de la Hunty, A. (2009). Early nutrition and later obesity: Animal models provide insights into mechanisms. Early Nutrition Programming and Health Outcomes in Later Life. Advances in Experimental Medicine and Biology, Srpinger.","DOI":"10.1007\/978-1-4020-9173-5"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8090","DOI":"10.3390\/nu7095384","article-title":"Early life nutrition and energy balance disorders in offspring in later life","volume":"7","author":"Reynolds","year":"2015","journal-title":"Nutrients"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Adair, L.S. (2014, January 27). Long-term consequences of nutrition and growth in early childhood and possible preventive interventions. Proceedings of the Nestle Nutrition Institute Workshop Series, Basel, Switzerland.","DOI":"10.1159\/000354949"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.cmet.2020.07.014","article-title":"Size Does Matter: Litter Size Strongly Determines Adult Metabolism in Rodents","volume":"32","author":"Lerin","year":"2020","journal-title":"Cell Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5617","DOI":"10.1210\/en.2010-0684","article-title":"Transgenerational inheritance of glucose intolerance in a mouse model of neonatal overnutrition","volume":"151","author":"Pentinat","year":"2010","journal-title":"Endocrinology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6025","DOI":"10.1096\/fj.201700717RR","article-title":"Epigenetic programming at the Mogat1 locus may link neonatal overnutrition with long-term hepatic steatosis and insulin resistance","volume":"32","author":"Pentinat","year":"2018","journal-title":"FASEB J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"101162","DOI":"10.1016\/j.molmet.2021.101162","article-title":"Neonatal overfeeding during lactation rapidly and permanently misaligns the hepatic circadian rhythm and programmes adult NAFLD","volume":"45","author":"Ribo","year":"2021","journal-title":"Mol. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1007\/s00125-020-05238-w","article-title":"Chrono-nutrition for the prevention and treatment of obesity and type 2 diabetes: From mice to men","volume":"63","author":"Hawley","year":"2020","journal-title":"Diabetologia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1016\/j.cmet.2014.11.001","article-title":"Time-restricted feeding is a preventative and therapeutic intervention against diverse nutritional challenges","volume":"20","author":"Chaix","year":"2014","journal-title":"Cell Metab."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101161","DOI":"10.1016\/j.isci.2020.101161","article-title":"Time-Restricted Eating: Benefits, Mechanisms, and Challenges in Translation","volume":"23","author":"Regmi","year":"2020","journal-title":"iScience"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.cmet.2016.06.001","article-title":"Fasting, Circadian Rhythms, and Time-Restricted Feeding in Healthy Lifespan","volume":"23","author":"Longo","year":"2016","journal-title":"Cell Metab."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1016\/j.cell.2013.11.034","article-title":"Reprogramming of the circadian clock by nutritional challenge","volume":"155","author":"Patel","year":"2013","journal-title":"Cell"},{"key":"ref_21","first-page":"236","article-title":"Time-Limited Eating in Pediatric Patients with Obesity-A Case Series","volume":"2","author":"Vidmar","year":"2020","journal-title":"J. Food Sci. Nutr. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"106082","DOI":"10.1016\/j.cct.2020.106082","article-title":"Time limited eating in adolescents with obesity (time LEAd): Study protocol","volume":"95","author":"Vidmar","year":"2020","journal-title":"Contemp. Clin. Trials"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"28536","DOI":"10.3402\/fnr.v60.28536","article-title":"Effect of long-term high-fat diet intake on peripheral insulin sensibility, blood pressure, and renal function in female rats","volume":"60","author":"Roza","year":"2016","journal-title":"Food Nutr. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/BF02905347","article-title":"Chronobiometry with pocket calculators and computer systems","volume":"10","author":"Halberg","year":"1980","journal-title":"Ric. Clin. Lab."},{"key":"ref_25","first-page":"397","article-title":"Inferential statistical methods for estimating and comparing cosinor parameters","volume":"9","author":"Bingham","year":"1982","journal-title":"Chronobiologia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3389\/fphys.2019.00423","article-title":"Circadian clock genes in the metabolism of non-alcoholic fatty liver disease","volume":"10","author":"Shi","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.cmet.2020.06.018","article-title":"Effects of 4- and 6-h Time-Restricted Feeding on Weight and Cardiometabolic Health: A Randomized Controlled Trial in Adults with Obesity","volume":"32","author":"Cienfuegos","year":"2020","journal-title":"Cell Metab."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"164","DOI":"10.4239\/wjd.v2.i10.164","article-title":"Epigenetic mechanisms involved in developmental nutritional programming","volume":"2","author":"Gabory","year":"2011","journal-title":"World J. Diabetes"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4643","DOI":"10.1038\/s41467-020-18412-w","article-title":"Time-restricted feeding alters lipid and amino acid metabolite rhythmicity without perturbing clock gene expression","volume":"11","author":"Lundell","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e1488","DOI":"10.1210\/clinem\/dgaa691","article-title":"New Horizons: Circadian Control of Metabolism Offers Novel Insight Into the Cause and Treatment of Metabolic Diseases","volume":"106","author":"Deota","year":"2021","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1111\/j.1582-4934.2010.01160.x","article-title":"Long-term restricted feeding alters circadian expression and reduces the level of inflammatory and disease markers","volume":"15","author":"Sherman","year":"2011","journal-title":"J. Cell. Mol. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1101\/gad.186858.112","article-title":"Rev-erb\u03b1 and Rev-erb\u03b2 coordinately protect the circadian clock and normal metabolic function","volume":"26","author":"Bugge","year":"2012","journal-title":"Genes Dev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1016\/j.celrep.2018.11.077","article-title":"Fasting Imparts a Switch to Alternative Daily Pathways in Liver and Muscle","volume":"25","author":"Kinouchi","year":"2018","journal-title":"Cell Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"556","DOI":"10.21037\/atm.2019.09.64","article-title":"Time-restricted feeding during childhood has persistent effects on mice commensal microbiota","volume":"7","author":"Hu","year":"2019","journal-title":"Ann. Transl. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1038\/s41390-018-0156-z","article-title":"Time-restricted feeding causes irreversible metabolic disorders and gut microbiota shift in pediatric mice","volume":"85","author":"Hu","year":"2019","journal-title":"Pediatr. Res."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/10\/3579\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:12:34Z","timestamp":1760166754000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/10\/3579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,13]]},"references-count":35,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["nu13103579"],"URL":"https:\/\/doi.org\/10.3390\/nu13103579","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,13]]}}}