{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T16:13:26Z","timestamp":1760890406984,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sone Farm Co., Ltd.","award":["No number"],"award-info":[{"award-number":["No number"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>We have recently demonstrated that the exopolysaccharides (EPSs) produced by a plant-derived lactic acid bacterium, Lactobacillus paracasei IJH-SONE68, prevent and ameliorate allergic reaction on contact in dermatitis model mice. In the present study, we conducted a clinical trial using a capsule containing spray-dried powder from pineapple juice broth fermented with the LAB strain as an experimental diet. The clinical trial was conducted as a double-blind and placebo-controlled randomized comparative study from May 2019 to July 2021. Males and females between the ages of 21 and 70 who experience chronic allergies participated in the study. Sixty subjects were instructed to orally take a capsule containing the IJH-SONE68 powder or placebo, every day for 12 weeks. After the clinical trial was over, the scores based on subjects\u2019 self-assessment of allergic status were significantly improved in the intervention group, as compared with the placebo group. Some serum biochemicals associated with inflammation response were also significantly improved by intake of the experimental diet. In conclusion, the IJH-SONE68-derived EPS improves chronic allergy status in humans and is expected to decrease their inconvenience.<\/jats:p>","DOI":"10.3390\/nu13114022","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T23:04:46Z","timestamp":1636671886000},"page":"4022","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Plant-Derived Lactobacillus paracasei IJH-SONE68 Improves Chronic Allergy Status: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial"],"prefix":"10.3390","volume":"13","author":[{"given":"Masafumi","family":"Noda","sequence":"first","affiliation":[{"name":"Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi 1\u20132\u20133, Minami-ku, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keishi","family":"Kanno","sequence":"additional","affiliation":[{"name":"Department of General Internal Medicine, Hiroshima University Hospital, Kasumi 1\u20132\u20133, Minami-Ku, Hiroshima 734-8551, Japan"},{"name":"Department of Clinical Pharmaceutical and Therapeutics, Hiroshima University, Kasumi 1\u20132\u20133, Minami-ku, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9524-3667","authenticated-orcid":false,"given":"Narandalai","family":"Danshiitsoodol","sequence":"additional","affiliation":[{"name":"Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi 1\u20132\u20133, Minami-ku, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiko","family":"Higashikawa","sequence":"additional","affiliation":[{"name":"Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi 1\u20132\u20133, Minami-ku, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi 1\u20132\u20133, Minami-ku, Hiroshima 734-8551, Japan"},{"name":"Department of Clinical Pharmaceutical and Therapeutics, Hiroshima University, Kasumi 1\u20132\u20133, Minami-ku, Hiroshima 734-8551, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Liva, G.A., Karatzanis, A.D., and Prokopakis, E.P. (2021). Review of rhinitis: Classification, types, pathophysiology. J. Clin. Med., 10.","key":"ref_1","DOI":"10.3390\/jcm10143183"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1600-065X.2011.01020.x","article-title":"Pathophysiology of allergic inflammation","volume":"242","author":"Barnes","year":"2011","journal-title":"Immunol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1039\/D0TB02590A","article-title":"Nanosystems as curative platforms for allergic disorder management","volume":"9","author":"Fan","year":"2021","journal-title":"J. Mater. Chem. B."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1016\/j.intimp.2011.07.005","article-title":"Allergic rhinitis: An update on disease, present treatments and future prospects","volume":"11","author":"Mandhane","year":"2011","journal-title":"Int. Immunopharmacol."},{"key":"ref_5","first-page":"23","article-title":"Epidemiology of rhinitis: Allergic and nonallergic","volume":"19","author":"Settipane","year":"2007","journal-title":"Clin. Allergy Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.alit.2020.04.001","article-title":"Japanese guidelines for allergic rhinitis 2020","volume":"69","author":"Okubo","year":"2020","journal-title":"Allergol. Int."},{"doi-asserted-by":"crossref","unstructured":"Zhang, H., and Cai, Y. (2014). Biodiversity of lactic acid bacteria. Bacteria Fundamentals and Practice, Springer.","key":"ref_7","DOI":"10.1007\/978-94-017-8841-0"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/nrmicro2937","article-title":"Bacteriocins\u2014a viable alternative to antibiotics?","volume":"11","author":"Cotter","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/ajcn\/80.2.245","article-title":"Yogurt and gut function","volume":"80","author":"Adolfsson","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"S58","DOI":"10.1086\/523341","article-title":"Probiotics: Definition, source, selection, and uses","volume":"46","author":"Sanders","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.nut.2009.05.008","article-title":"Improvement of constipation and liver function by plant-derived lactic acid bacteria: A double-blind, randomized trial","volume":"26","author":"Higashikawa","year":"2010","journal-title":"Nutrition"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/ejcn.2016.17","article-title":"Antiobesity effect of Pediococcus pentosaceus LP28 on overweight subjects: A randomized, double-blind, placebo-controlled clinical trial","volume":"70","author":"Higashikawa","year":"2016","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"605","DOI":"10.26502\/acbr.50170129","article-title":"Lactobacillus plantarum SN13T cells improve hepatic dysfunction and fecal microbiota: A randomized pilot study","volume":"4","author":"Higashikawa","year":"2020","journal-title":"Arch. Clin. Biomed. Res."},{"key":"ref_14","first-page":"271","article-title":"Characterization of the LP28 strain-specific exopolysaccharide biosynthetic gene cluster found in the whole circular genome of Pediococcus pentosaceus","volume":"5","author":"Yasutake","year":"2016","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1248\/bpb.b16-00856","article-title":"Characterization of exopolysaccharides produced by thermophilic lactic acid bacteria isolated from tropical fruits of Thailand","volume":"40","author":"Panthavee","year":"2017","journal-title":"Biol. Pharm. Bull."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1248\/bpb.b17-00840","article-title":"Characterization of the SN35N strain-specific exopolysaccharide encoded in the whole circular genome of a plant-derived Lactobacillus plantarum","volume":"41","author":"Noda","year":"2018","journal-title":"Biol. Pharm. Bull."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/jb\/mvy048","article-title":"A novel structure of exopolysaccharide produced by a plant-derived lactic acid bacterium Lactobacillus paracasei IJH-SONE68","volume":"164","author":"Noda","year":"2018","journal-title":"J. Biochem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1002\/cm.20544","article-title":"Interaction of low molecular weight hyaluronan with CD44 and toll-like receptors promotes the actin filament-associated protein 110-actin binding and MyD88-NF\u03baB signaling leading to proinflammatory cytokine\/chemokine production and breast tumor invasion","volume":"68","author":"Bourguignon","year":"2011","journal-title":"Cytoskeleton"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.cellbio.23.090506.123337","article-title":"Hyaluronan in tissue injury and repair","volume":"23","author":"Jiang","year":"2007","journal-title":"Annu. Rev. Cell. Dev. Biol."},{"key":"ref_20","first-page":"78","article-title":"Hyaluronic acid in inflammation and tissue regeneration","volume":"28","author":"Litwiniuk","year":"2010","journal-title":"Wounds"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4589","DOI":"10.1074\/jbc.R100038200","article-title":"Signaling properties of hyaluronan receptors","volume":"277","author":"Turley","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0021-9258(19)41982-0","article-title":"Purification and properties of hyaluronidase from bull sperm","volume":"250","author":"Yang","year":"1975","journal-title":"J. Biol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1248\/cpb.33.642","article-title":"Activation of hyaluronidase by metallic salts and compound 48\/80, and inhibitory effect of anti-allergic agents on hyaluronidase","volume":"33","author":"Kakegawa","year":"1985","journal-title":"Chem. Pharm. Bull."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1023\/A:1012592620347","article-title":"Inhibitory effects of microalgae on the activation of hyaluronidase","volume":"13","author":"Fujitani","year":"2001","journal-title":"J. Appl. Phycol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3358\/shokueishi.31.233","article-title":"Inhibitory effect of tea extracts on hyaluronidase","volume":"31","author":"Maeda","year":"1990","journal-title":"Shokuhin Eiseigaku Zashi"},{"doi-asserted-by":"crossref","unstructured":"Noda, M., Sultana, N., Hayashi, I., Fukamachi, M., and Sugiyama, M. (2019). Exopolysaccharide produced by Lactobacillus paracasei IJH-SONE68 prevents and improves the picryl chloride-induced contact dermatitis. Molecules, 24.","key":"ref_26","DOI":"10.3390\/molecules24162970"},{"doi-asserted-by":"crossref","unstructured":"Noda, M., Danshiitsoodol, N., Kanno, K., Uchida, T., and Sugiyama, M. (2021). The exopolysaccharide produced by Lactobacillus paracasei IJH-SONE68 prevents and ameliorates inflammatory responses in DSS\u2013induced ulcerative colitis. Microorganisms, 9.","key":"ref_27","DOI":"10.3390\/microorganisms9112243"},{"key":"ref_28","first-page":"21","article-title":"Standard questionnaire for QOL of Japanese patients with allergic rhinitis","volume":"52","author":"Okuda","year":"2003","journal-title":"Allergy"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1038\/s41440-019-0284-9","article-title":"The Japanese Society of Hypertension Guidelines for the Management of Hypertension (JSH 2019)","volume":"42","author":"Umehara","year":"2019","journal-title":"Hypertens. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1001\/jama.2015.15281","article-title":"Multiple imputation: A flexible tool for handling missing data","volume":"314","author":"Li","year":"2015","journal-title":"JAMA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1136\/bmj.39304.678194.AE","article-title":"Perennial rhinitis","volume":"335","author":"Saleh","year":"2007","journal-title":"BMJ"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.2165\/00151829-200403010-00006","article-title":"Etiopathogenesis and management of perennial allergic rhinitis: A state-of-the-art review","volume":"3","author":"Bush","year":"2004","journal-title":"Treat. Respir. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1254\/jjp.59.201","article-title":"Active cutaneous anaphylaxis (ACA) in the mouse ear","volume":"59","author":"Inagaki","year":"1992","journal-title":"Jpn. J. Pharmacol."},{"unstructured":"Noda, M., and Sugiyama, M. (2021). Studies aiming for the development of medicines to treat inflammatory disorders. Seibutsu-kogaku Kaishi, 99, in press.","key":"ref_34"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6735","DOI":"10.1158\/1078-0432.CCR-07-4843","article-title":"The interleukin-8 pathway in cancer","volume":"14","author":"Waugh","year":"2008","journal-title":"Clin. Cancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.cell.2008.03.038","article-title":"Chemokine signaling via the CXCR2 receptor reinforces senescence","volume":"133","author":"Acosta","year":"2008","journal-title":"Cell"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1159\/000514885","article-title":"Interleukin 8 elicits rapid physiological changes in neutrophils that are altered by inflammatory conditions","volume":"13","author":"Bernhard","year":"2021","journal-title":"J. Innate Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1503\/cmaj.1040752","article-title":"Liver enzyme alteration: A guide for clinicians","volume":"172","author":"Giannini","year":"2005","journal-title":"CMAJ"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s12291-013-0408-y","article-title":"Alkaline phosphatase: An overview","volume":"29","author":"Sharma","year":"2014","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3892\/br.2013.60","article-title":"Assessment of the value of serum cholinesterase as a liver function test for cirrhotic patients","volume":"1","author":"Meng","year":"2013","journal-title":"Biomed. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00592-011-0309-6","article-title":"Metabolic syndrome: Its history, mechanisms, and limitations","volume":"49","author":"Oda","year":"2012","journal-title":"Acta Diabetol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.atherosclerosis.2015.04.804","article-title":"Associations between serum cholinesterase and incident hyper-LDL cholesterolemia, hypertriglyceridemia and hypo-HDL cholesterolemia as well as changes in lipid levels in a health screening population","volume":"241","author":"Oda","year":"2015","journal-title":"Atherosclerosis"}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/11\/4022\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:28:35Z","timestamp":1760167715000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/11\/4022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,11]]},"references-count":42,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["nu13114022"],"URL":"https:\/\/doi.org\/10.3390\/nu13114022","relation":{},"ISSN":["2072-6643"],"issn-type":[{"type":"electronic","value":"2072-6643"}],"subject":[],"published":{"date-parts":[[2021,11,11]]}}}