{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:51:23Z","timestamp":1790445083845,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T00:00:00Z","timestamp":1637366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Program of Scienti\ufb01c Research \u201cChemical Technologies and Materials\u201d, the subprogram \u201cPharmacology and Pharmacy\u201d (the Republic of Belarus)","award":["grant No 5.33"],"award-info":[{"award-number":["grant No 5.33"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The aim of this study was to evaluate the therapeutic effects of chlorogenic acid (CGA) in rats with advanced alcoholic steatohepatitis. The rats were fed on a high-fat diet and gavaged with ethanol (4 g\/kg) for 8 weeks. The livers of ethanol-treated rats showed steatosis; necrosis and mononuclear infiltration; and significant upregulation of the mRNA expression of the prooxidant (Cyp2e1, iNos), lipogenic (Srebp1, Acc), proinflammatory (Tlr4, Nf-\u03bab, TnfA, Il-1B, and Il-6), and profibrogenic (TgfB, Col1, VegfA) genes. Simultaneously, a downregulation of level of Sod and Nrf2 was observed, which was accompanied by increased serum transaminase, TnfA, and serum and liver triglycerides levels. CGA administration (40 and 80 mg\/kg, 8 weeks) to ethanol-fed group reduced the liver expression levels of Cyp2e1 and iNos, whereas it markedly enhanced the expression of Sod, Nrf2, and Ho-1. CGA at both doses downregulated the expressions of lipogenic, proinflammatory, and profibrogenic genes, while the expression of Tlr4 was lowered only after the higher dose of CGA. The higher dose of CGA efficiently prevented the progression of alcohol-induced steatosis and reduced inflammation through regulation of the expression of genes encoding the proteins involved in the Tlr4\/Nf-\u03baB signaling pathway and fibrosis. The study revealed hepatoprotective and anti-inflammatory effects of CGA through the regulation of expression of genes encoding Cyp2e1\/Nrf2 involved in oxidative stress modulation. These results demonstrate CGA as a therapeutic candidate for the prevention and treatment of alcoholic steatohepatitis.<\/jats:p>","DOI":"10.3390\/nu13114155","type":"journal-article","created":{"date-parts":[[2021,11,21]],"date-time":"2021-11-21T21:00:50Z","timestamp":1637528450000},"page":"4155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Chlorogenic Acid Protects against Advanced Alcoholic Steatohepatitis in Rats via Modulation of Redox Homeostasis, Inflammation, and Lipogenesis"],"prefix":"10.3390","volume":"13","author":[{"given":"Vyacheslav","family":"Buko","sequence":"first","affiliation":[{"name":"Division of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, Belarus"},{"name":"Department of Biotechnology, University of Medical Sciences, Krakowska 9, 15-875 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6130-787X","authenticated-orcid":false,"given":"Ilya","family":"Zavodnik","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Yanka Kupala State University of Grodno, Ozheshko 22, 230023 Grodno, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8050-3702","authenticated-orcid":false,"given":"Gra\u017cyna","family":"Budryn","sequence":"additional","affiliation":[{"name":"Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 2\/22, 90-537 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8341-1654","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Zak\u0142os-Szyda","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2\/22, 90-537 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Belonovskaya","sequence":"additional","affiliation":[{"name":"Division of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siarhei","family":"Kirko","sequence":"additional","affiliation":[{"name":"Division of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0989-0671","authenticated-orcid":false,"given":"Dorota","family":"\u017by\u017celewicz","sequence":"additional","affiliation":[{"name":"Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 2\/22, 90-537 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Zakrzeska","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, University of Medical Sciences, Krakowska 9, 15-875 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8717-1918","authenticated-orcid":false,"given":"Aliaksei","family":"Bakunovich","sequence":"additional","affiliation":[{"name":"Division of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"Rusin","sequence":"additional","affiliation":[{"name":"The 2nd Department of Surgical Diseases, Grodno State Medical University, Gorkogo 80, 230009 Grodno, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Moroz","sequence":"additional","affiliation":[{"name":"Division of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3748\/wjg.v22.i1.8","article-title":"Optimal management for alcoholic liver disease: Conventional medications, natural therapy or combination?","volume":"22","author":"Kim","year":"2016","journal-title":"World J. 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