{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:28:36Z","timestamp":1775644116936,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008114","name":"University of Nebraska-Lincoln","doi-asserted-by":"publisher","award":["Frank E. Mussehl and Inez L. Mussehl Poultry Research Fund (Agricultural Research Division)"],"award-info":[{"award-number":["Frank E. Mussehl and Inez L. Mussehl Poultry Research Fund (Agricultural Research Division)"]}],"id":[{"id":"10.13039\/100008114","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Hen breed, diet enrichment, cooking methods, and gastrointestinal (GI) digestion modulates the bioaccessibility of the bioactive compounds in eggs, but their synergistic role in modulating bioactivity is still unclear. The present study evaluates the effect of hen breed, diet enrichment, and GI digestion on the cooked whole egg-derived peptides in-vitro antioxidant and antihypertensive activities. Standard and enriched whole eggs from White Leghorn (WLH) and Rhode Island Red (RIR) hens were boiled or fried and subjected to GI digestion. Antioxidant activity was measured through oxygen radical absorbance capacity (ORAC) and gastrointestinal epithelial cell-based assays, and the antihypertensive capacity by in-vitro Angiotensin-I Converting Enzyme (ACE) inhibition assay. WLH fried standard egg hydrolysate showed a high ORAC antioxidant activity but failed to show any significant antioxidant effect in the cell-based assay. No significant differences were observed in the antihypertensive activity, although enriched samples tended to have a higher ACE-inhibitory capacity. The peptide profile explained the antioxidant capacities based on antioxidant structural requirements from different peptide fractions, while previously reported antihypertensive peptides were found in all samples. The study validates the importance of physiologically relevant models and requires future studies to confirm mechanisms that yield bioactive compounds in whole egg hydrolysates.<\/jats:p>","DOI":"10.3390\/nu13124232","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T23:22:56Z","timestamp":1638314576000},"page":"4232","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bioactivity of Cooked Standard and Enriched Whole Eggs from White Leghorn and Rhode Island Red in Exhibiting In-Vitro Antioxidant and ACE-Inhibitory Effects"],"prefix":"10.3390","volume":"13","author":[{"given":"Emerson","family":"Nolasco","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE 68588-6205, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mike","family":"Naldrett","sequence":"additional","affiliation":[{"name":"Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588-0665, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588-0665, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip E.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE 68588-6205, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2094-7835","authenticated-orcid":false,"given":"Kaustav","family":"Majumder","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE 68588-6205, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102178","DOI":"10.1016\/j.plefa.2020.102178","article-title":"Lipidomic characterization of omega-3 polyunsaturated fatty acids in phosphatidylcholine and phosphatidylethanolamine species of egg yolk lipid derived from hens fed flaxseed oil and marine algal biomass","volume":"161","author":"Neijat","year":"2020","journal-title":"Prostagland. Leukot. Essent. Fat. Acids"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1080\/1745039X.2019.1586500","article-title":"Flaxseed and dried tomato waste used together in laying hens diet","volume":"73","author":"Panaite","year":"2019","journal-title":"Arch. Anim. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1365-2621.2004.tb10727.x","article-title":"Functional Foods: Their Role in Health Promotion and Disease Prevention","volume":"69","author":"Shahidi","year":"2004","journal-title":"J. Food Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s10198-017-0934-2","article-title":"The effectiveness and cost-effectiveness of plant sterol or stanol-enriched functional foods as a primary prevention strategy for people with cardiovascular disease risk in England: A modeling study","volume":"19","author":"Yang","year":"2018","journal-title":"Eur. J. Health Econ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.psj.2020.10.001","article-title":"Dietary green tea powder supplementation enriched egg nutrients and physicochemical property in an indigenous chicken breed","volume":"100","author":"Chen","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1021\/jf8028557","article-title":"Angiotensin I Converting Enzyme Inhibitory Peptides from Simulated in vitro Gastrointestinal Digestion of Cooked Eggs","volume":"57","author":"Majumder","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.foodres.2015.10.015","article-title":"Innovative applications of high-intensity ultrasound in the development of functional food ingredients: Production of protein hydrolysates and bioactive peptides","volume":"77","author":"Ozuna","year":"2015","journal-title":"Food Res. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"777","DOI":"10.3168\/jds.S0022-0302(95)76689-9","article-title":"Purification and Characterization of Angiotensin I-Converting Enzyme Inhibitors from Sour Milk","volume":"78","author":"Nakamura","year":"1995","journal-title":"J. Dairy Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.4315\/0362-028X-67.9.1914","article-title":"Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis","volume":"67","author":"Miguel","year":"2004","journal-title":"J. Food Prot."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"128623","DOI":"10.1016\/j.foodchem.2020.128623","article-title":"Evaluating the Effect of Cooking and Gastrointestinal Digestion in Modulating the Bio-Accessibility of Different Bioactive Compounds of Eggs","volume":"344","author":"Nolasco","year":"2021","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jff.2016.12.005","article-title":"Angiotensin converting enzyme (ACE) inhibitory peptides derived from the simulated in vitro gastrointestinal digestion of cooked chicken breast","volume":"29","author":"Sangsawad","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.foodchem.2011.04.058","article-title":"Free aromatic amino acids in egg yolk show antioxidant properties","volume":"129","author":"Nimalaratne","year":"2011","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Jahandideh, F., Majumder, K., Chakrabarti, S., Morton, J.S., Panahi, S., Kaufman, S., Davidge, S.T., and Wu, J. (2014). Beneficial effects of simulated gastro-intestinal digests of fried egg and its fractions on blood pressure, plasma lipids and oxidative stress in spontaneously hypertensive rats. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115006"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1039\/C4FO00204K","article-title":"Antioxidant activity in cooked and simulated digested eggs","volume":"5","author":"Remanan","year":"2014","journal-title":"Food Funct."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1021\/jf505615w","article-title":"Bioaccessibility and Digestive Stability of Carotenoids in Cooked Eggs Studied Using a Dynamic in Vitro Gastrointestinal Model","volume":"63","author":"Nimalaratne","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.foodchem.2015.07.116","article-title":"Effects of storage and cooking on the antioxidant capacity of laying hen eggs","volume":"194","author":"Nimalaratne","year":"2016","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1006\/abbi.2000.2149","article-title":"Antioxidant and prooxidant properties of carotenoids","volume":"385","author":"Young","year":"2001","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jff.2012.10.004","article-title":"Fried egg digest decreases blood pressure in spontaneous hypertensive rats","volume":"5","author":"Majumder","year":"2013","journal-title":"J. Funct. Foods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jnutbio.2018.09.019","article-title":"Isolation and identification of peptides from simulated gastrointestinal digestion of preserved egg white and their anti-inflammatory activity in TNF-\u03b1-induced Caco-2 cells","volume":"63","author":"Zhang","year":"2019","journal-title":"J. Nutr. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1021\/jf051263l","article-title":"Structural requirements of angiotensin I-converting enzyme inhibitory peptides: Quantitative structure-activity relationship study of di- and tripeptides","volume":"54","author":"Wu","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Deng, B., Long, H., Tang, T., Ni, X., Chen, J., Yang, G., Zhang, F., Cao, R., Cao, D., and Zeng, M. (2019). Quantitative structure-activity relationship study of antioxidant tripeptides based on model population analysis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20040995"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.jff.2015.12.003","article-title":"Structure-activity relationship of antioxidant dipeptides: Dominant role of Tyr, Trp, Cys and Met residues","volume":"21","author":"Zheng","year":"2016","journal-title":"J. Funct. Foods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"12373","DOI":"10.1038\/s41598-017-12019-w","article-title":"Lecithin derived from \u03c9-3 PUFA fortified eggs decreases blood pressure in spontaneously hypertensive rats","volume":"7","author":"Nowacki","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion method suitable for food-an international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.foodres.2015.12.002","article-title":"In vitro digestion of foods using pH-stat and the INFOGEST protocol: Impact of matrix structure on digestion kinetics of macronutrients, proteins and lipids","volume":"88","author":"Mat","year":"2016","journal-title":"Food Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jff.2018.12.013","article-title":"Antioxidant and anti-inflammatory polyphenols and peptides of common bean (Phaseolus vulga L.) milk and yogurt in Caco-2 and HT-29 cell models","volume":"53","author":"Chen","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8896","DOI":"10.1021\/jf0715166","article-title":"Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements","volume":"55","author":"Wolfe","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.jff.2016.01.004","article-title":"Anthocyanin-rich phenolic extracts of purple root vegetables inhibit pro-inflammatory cytokines induced by H2O2 and enhance antioxidant enzyme activities in Caco-2 cells","volume":"22","author":"Zhang","year":"2016","journal-title":"J. Funct. Foods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"195305","DOI":"10.1155\/2014\/195305","article-title":"Wild mushrooms in Nepal: Some potential candidates as antioxidant and ACE-inhibition sources","volume":"2014","author":"Suhara","year":"2014","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.2116\/analsci.24.1057","article-title":"Assay of angiotensin I-converting enzyme-inhibiting activity based on the detection of 3-hydroxybutyrate with water-soluble tetrazolium salt","volume":"24","author":"Lam","year":"2008","journal-title":"Anal. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2508","DOI":"10.1080\/10408398.2017.1329704","article-title":"Bioactive peptides derived from egg proteins: A review","volume":"58","author":"Liu","year":"2018","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8452","DOI":"10.1021\/jf402256k","article-title":"Synergistic inhibition of lipid oxidation by pea protein hydrolysate coupled with licorice extract in a liposomal model system","volume":"61","author":"Zhang","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Shahidi, F. (2015). Amino acids, peptides, and proteins as antioxidants for food preservation. Handbook of Antioxidants for Food Preservation, Elsevier Ltd.","DOI":"10.1016\/B978-1-78242-089-7.00001-4"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1002\/qsar.200630005","article-title":"Structural requirements of angiotensin I-converting enzyme inhibitory peptides: Quantitative structure-activity relationship modeling of peptides containing 4\u201310 amino acid residues","volume":"25","author":"Wu","year":"2006","journal-title":"QSAR Comb. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Rao, P.S., Nolasco, E., Handa, A., Naldrett, M.J., Alvarez, S., and Majumder, K. (2020). Effect of pH and heat treatment on the antioxidant activity of egg white protein-derived peptides after simulated in-vitro gastrointestinal digestion. Antioxidants, 9.","DOI":"10.3390\/antiox9111114"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1021\/acs.jafc.5b04230","article-title":"Antioxidant Peptides Identified from Ovotransferrin by the ORAC Method Did Not Show Anti-Inflammatory and Antioxidant Activities in Endothelial Cells","volume":"64","author":"Jahandideh","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.lwt.2017.08.026","article-title":"Protein breakdown and release of antioxidant peptides during simulated gastrointestinal digestion and the absorption by everted intestinal sac of rapeseed proteins","volume":"86","author":"Dai","year":"2017","journal-title":"LWT"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Guha, S., Alvarez, S., and Majumder, K. (2021). Transport of Dietary Anti-Inflammatory Peptide, \u03b3-Glutamyl Valine (\u03b3-EV), across the Intestinal Caco-2 Monolayer. Nutrients, 13.","DOI":"10.3390\/nu13051448"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jfca.2017.01.011","article-title":"Transepithelial transport of lunasin and derived peptides: Inhibitory effects on the gastrointestinal cancer cells viability","volume":"68","author":"Recio","year":"2018","journal-title":"J. Food Compos. Anal."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.foodchem.2007.04.067","article-title":"Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH)","volume":"106","author":"Li","year":"2008","journal-title":"Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"681","DOI":"10.3109\/00365521.2014.898326","article-title":"Surface area of the digestive tract-revisited","volume":"49","author":"Helander","year":"2014","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.foodchem.2015.05.014","article-title":"Purification and characterization of antioxidant peptides from enzymatically hydrolyzed chicken egg white","volume":"188","author":"Nimalaratne","year":"2015","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/s41538-018-0015-7","article-title":"Purification and characterization of antioxidant peptides from cooked eggs using a dynamic in vitro gastrointestinal model in vascular smooth muscle A7r5 cells","volume":"2","author":"Wang","year":"2018","journal-title":"npj Sci. Food"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.foodchem.2014.12.063","article-title":"Influence of peptides-phenolics interaction on the antioxidant profile of protein hydrolysates from Brassica napus","volume":"178","author":"Vioque","year":"2015","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.foodchem.2010.04.083","article-title":"Oxygen Radical Absorbance Capacity of Peptides from Egg White Protein Ovotransferrin and their Interaction with Phytochemicals","volume":"123","author":"Huang","year":"2010","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/s41596-018-0119-1","article-title":"INFOGEST static in vitro simulation of gastrointestinal food digestion","volume":"14","author":"Brodkorb","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41538-018-0033-5","article-title":"Rapeseed protein-derived peptides, LY, RALP, and GHS, modulates key enzymes and intermediate products of renin\u2013angiotensin system pathway in spontaneously hypertensive rat","volume":"3","author":"He","year":"2019","journal-title":"npj Sci. Food"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.foodchem.2013.02.111","article-title":"Interactions of dietary fibre and omega-3-rich oil with protein in surimi gels developed with salt substitute","volume":"141","author":"Debusca","year":"2013","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/0305-0491(91)90342-B","article-title":"Studies on the carotenoids in the muscle of salmon-V. Combination of astaxanthin and canthaxanthin with bovine serum albumin and egg albumin","volume":"99","author":"Henmi","year":"1991","journal-title":"Comp. Biochem. Physiol. Part B Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"8950","DOI":"10.1021\/jf071687a","article-title":"Impact of fatty acyl composition and quantity of triglycerides on bioaccessibility of dietary carotenoids","volume":"55","author":"Huo","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_51","first-page":"1988","article-title":"Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships","volume":"90","author":"Roque","year":"2010","journal-title":"J. Sci. Food Agric."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/S0955-2863(98)00036-9","article-title":"Isolation and characterization of angiotensin I-converting enzyme inhibitor dipeptides derived from Allium sativum L (garlic)","volume":"9","author":"Suetsuna","year":"1998","journal-title":"J. Nutr. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"620609","DOI":"10.1155\/2012\/620609","article-title":"Modeling the QSAR of ACE-inhibitory peptides with ANN and its applied illustration","volume":"2012","author":"He","year":"2012","journal-title":"Int. J. Pept."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3831","DOI":"10.2174\/138161212802083707","article-title":"Molecular Link Mechanisms between Inflammation and Cancer","volume":"18","author":"Carvalho","year":"2012","journal-title":"Curr. Pharm. Des."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.foodres.2010.04.027","article-title":"A New Approach for Identification of Novel Antihypertensive Peptides from Egg Proteins by QSAR and Bioinformatics","volume":"43","author":"Majumder","year":"2010","journal-title":"Food Res. Int."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3847","DOI":"10.1039\/C7FO00839B","article-title":"Egg-derived bioactive peptides with ACE-inhibitory properties: A literature update","volume":"8","author":"Grootaert","year":"2017","journal-title":"Food Funct."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.regpep.2006.11.029","article-title":"Vasodilator effects of peptides derived from egg white proteins","volume":"140","author":"Miguel","year":"2007","journal-title":"Regul. Pept."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/12\/4232\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:35:48Z","timestamp":1760168148000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/12\/4232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":57,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["nu13124232"],"URL":"https:\/\/doi.org\/10.3390\/nu13124232","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]}}}