{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:02:23Z","timestamp":1791385343091,"version":"4.3.3"},"reference-count":45,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T00:00:00Z","timestamp":1640563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004946","name":"National Chung Hsing University","doi-asserted-by":"publisher","award":["(TCVGH-NCHU1107607"],"award-info":[{"award-number":["(TCVGH-NCHU1107607"]}],"id":[{"id":"10.13039\/501100004946","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Magnesium lithospermate B (MLB) is a primary hydrophilic component of Danshen, the dried root of Salvia miltiorrhiza used in traditional medicine, and its beneficial effects on obesity-associated metabolic abnormalities were reported in our previous study. The present study investigated the anti-muscle atrophy potential of MLB in mice with high-fat diet (HFD)-induced obesity. In addition to metabolic abnormalities, the HFD mice had a net loss of skeletal muscle weight and muscle fibers and high levels of muscle-specific ubiquitin E3 ligases, namely the muscle atrophy F-box (MAFbx) and muscle RING finger protein 1 (MuRF-1). MLB supplementation alleviated those health concerns. Parallel changes were revealed in high circulating tumor necrosis factor-\u03b1 (TNF-\u03b1) and interleukin-6 (IL-6), skeletal TNF receptor I (TNFRI), nuclear factor-kappa light chain enhancer of activated B cells (NF-\u03baB), p65 phosphorylation, and Forkhead box protein O1 (FoxO1) as well as low skeletal phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) phosphorylation. The study revealed that MLB prevented obesity-associated skeletal muscle atrophy, likely through the inhibition of MAFbx\/MuRF-1-mediated muscular degradation. The activation of the PI3K-Akt-FoxO1 pathway and inhibition of the TNF-\u03b1\/TNFRI\/NF-\u03baB pathway were assumed to be beneficial effects of MLB.<\/jats:p>","DOI":"10.3390\/nu14010104","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T01:20:43Z","timestamp":1640654443000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Magnesium Lithospermate B Attenuates High-Fat Diet-Induced Muscle Atrophy in C57BL\/6J Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Tsun-Li","family":"Cheng","sequence":"first","affiliation":[{"name":"Veterinary Medical Teaching Hospital, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi-Yun","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng-Ying","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Chi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1123-0015","authenticated-orcid":false,"given":"Cian-Fen","family":"Jhuo","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin-Ho","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"},{"name":"Department of Pediatrics, Tungs\u2019 Taichung Metro Harbor Hospital, Taichung 435, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, Taichung 407, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8991-6394","authenticated-orcid":false,"given":"Yu-Hsiang","family":"Kuan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5105-5285","authenticated-orcid":false,"given":"Wen-Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Veterinary Medical Teaching Hospital, National Chung Hsing University, Taichung 402, Taiwan"},{"name":"Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/2046-2395-3-9","article-title":"It is not just muscle mass: A review of muscle quality, composition and metabolism during ageing as determinants of muscle function and mobility in later life","volume":"3","author":"McGregor","year":"2014","journal-title":"Longev. 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