{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:51:07Z","timestamp":1791589867216,"version":"4.3.4"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T00:00:00Z","timestamp":1642982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science, Research and Innovation Fund (NSRF) and Prince of Songkla University","award":["SCI6405072S"],"award-info":[{"award-number":["SCI6405072S"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Constipation is a symptom that is widely found in the world\u2019s population. Various dietary supplementations are used to relieve and prevent constipation. Seaweed is widely used for its health benefits. In this study, we aimed to investigate the effects of Sargassum plagiophyllum extract (SPE) on functions of the gastrointestinal tract and gut microbiota. The results show that SPE pretreatment increased the frequency of gut contraction, leading to reduce gut transit time. SPE pretreatment also significantly increased the secretion of Cl\u2212 and reduced Na+ absorption, increasing fecal water content in constipated mice (p &lt; 0.05). In addition, the Bifidobacteria population in cecal contents was significantly higher in constipated mice pretreated with 500 mg\/kg SPE for 14 days than in untreated constipated mice (p &lt; 0.05). Our findings suggest that SPE can prevent constipation in loperamide-induced mice. This study may be useful for the development of human food supplements from S. plagiophyllum, which prevent constipation.<\/jats:p>","DOI":"10.3390\/nu14030496","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T21:07:11Z","timestamp":1643144831000},"page":"496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Sargassum plagiophyllum Extract Enhances Colonic Functions and Modulates Gut Microbiota in Constipated Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Pissared","family":"Khuituan","sequence":"first","affiliation":[{"name":"Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawiya","family":"Huipao","sequence":"additional","affiliation":[{"name":"Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilobon","family":"Jeanmard","sequence":"additional","affiliation":[{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitthiwach","family":"Thantongsakul","sequence":"additional","affiliation":[{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warittha","family":"Promjun","sequence":"additional","affiliation":[{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwarat","family":"Chuthong","sequence":"additional","affiliation":[{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chittipong","family":"Tipbunjong","sequence":"additional","affiliation":[{"name":"Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1233-9488","authenticated-orcid":false,"given":"Saranya","family":"Peerakietkhajorn","sequence":"additional","affiliation":[{"name":"Gut Biology and Microbiota Research Unit, Prince of Songkla University, Songkhla 90110, Thailand"},{"name":"Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"677","DOI":"10.5056\/jnm15187","article-title":"Prevalence and self-recognition of chronic constipation: Results of an internet survey","volume":"22","author":"Tamura","year":"2016","journal-title":"J. Neurogastroenterol. Motil."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"117418","DOI":"10.1016\/j.carbpol.2020.117418","article-title":"Prevention of loperamide induced constipation in mice by KGM and the mechanisms of different gastrointestinal tract microbiota regulation","volume":"256","author":"Zhang","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.mpmed.2019.10.005","article-title":"Constipation and malignant bowel obstruction in palliative care","volume":"48","author":"Boland","year":"2020","journal-title":"Medicine"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Milosavljevic, T., Popovic, D.D., Mijac, D.D., Milovanovic, T., Krstic, S., and Krstic, M.N. (2021). Chronic constipation, gastroenterohepatologist\u2019s approach. Dig. Dis.","DOI":"10.1159\/000516976"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e10631","DOI":"10.1097\/MD.0000000000010631","article-title":"Chronic constipation","volume":"97","author":"Forootan","year":"2018","journal-title":"Medicine"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1039\/C9FO00132H","article-title":"Protective effect of mulberry (Morus atropurpurea) fruit against diphenoxylate-induced constipation in mice through the modulation of gut microbiota","volume":"10","author":"Hu","year":"2019","journal-title":"Food Funct."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/1475-2891-10-19","article-title":"Is Bifidobacterium breve effective in the treatment of childhood constipation? Results from a pilot study","volume":"10","author":"Tabbers","year":"2011","journal-title":"Nutr. J."},{"key":"ref_8","first-page":"107","article-title":"Effect of a cell preparation of Enterococcus faecalis strain EC-12 on digesta flow and recovery from constipation in a pig model and human subjects","volume":"17","author":"Tsukahara","year":"2005","journal-title":"Microb. Ecol. Health Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1186\/s40064-016-2821-1","article-title":"Intestinal microbiota and chronic constipation","volume":"5","author":"Zhao","year":"2016","journal-title":"SpringerPlus"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1111\/j.1365-2036.2012.05007.x","article-title":"Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome","volume":"35","author":"Chassard","year":"2012","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1365-2982.2011.01803.x","article-title":"Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome","volume":"24","author":"Parkes","year":"2012","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1111\/j.1758-2229.2012.00327.x","article-title":"Structural alterations of faecal and mucosa-associated bacterial communities in irritable bowel syndrome","volume":"4","author":"Durban","year":"2012","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1055\/s-0032-1301754","article-title":"Medical Management of Constipation","volume":"25","author":"Portalatin","year":"2012","journal-title":"Clin. Colon Rectal Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1177\/2050640616663439","article-title":"Management of chronic constipation in adults","volume":"5","author":"Krogh","year":"2017","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1016\/j.cell.2018.05.008","article-title":"Transient osmotic perturbation causes long-term alteration to the gut microbiota","volume":"173","author":"Tropini","year":"2018","journal-title":"Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41575-019-0222-y","article-title":"Management of functional constipation in children and adults","volume":"17","author":"Vriesman","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ngatu, R.N., Ikeda, M., Watanabe, H., Tanaka, M., and Inoue, M. (2017). Laxative effects of dietary supplementation with sujiaonori algal biomaterial in Japanese adult women with functional constipation: A case study. J. Funct. Biomater., 8.","DOI":"10.3390\/jfb8020015"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Pe\u00f1alver, R., Lorenzo, J.M., Ros, G., Amarowicz, R., Pateiro, M., and Nieto, G. (2020). Seaweeds as a functional ingredient for a healthy diet. Mar. Drugs, 18.","DOI":"10.3390\/md18060301"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"012040","DOI":"10.1088\/1742-6596\/1542\/1\/012040","article-title":"Nutritional profile of Sargassum sp. from Pane Island, Tapanuli Tengah as a component of functional food","volume":"1542","author":"Dewinta","year":"2020","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"104021","DOI":"10.1016\/j.jff.2020.104021","article-title":"Oligosaccharides from Gracilaria fisheri ameliorate gastrointestinal dysmotility and gut dysbiosis in colitis mice","volume":"71","author":"Peerakietkhajorn","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4103\/2221-1691.326098","article-title":"Gracilaria fisheri oligosaccharides ameliorate inflammation and colonic epithelial barrier dysfunction in mice with acetic acid-induced colitis","volume":"11","author":"Siringoringo","year":"2021","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"012043","DOI":"10.1088\/1755-1315\/139\/1\/012043","article-title":"Prebiotic index evaluation of crude laminaran of Sargassum sp. using feces of wistar rats","volume":"139","author":"Chamidah","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anaerobe.2011.08.003","article-title":"In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds","volume":"18","author":"Ramnani","year":"2012","journal-title":"Anaerobe"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3224","DOI":"10.1039\/C6FO00309E","article-title":"Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae","volume":"7","author":"Shang","year":"2016","journal-title":"Food Funct."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1007\/s10811-016-0859-7","article-title":"An aqueous extract from Sargassum sp. enhances the immune response and resistance against Streptococcus iniae in the Asian sea bass (Lates calcarifer Bloch)","volume":"28","author":"Yangthong","year":"2016","journal-title":"J. Appl. Phycol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.ijbiomac.2020.02.168","article-title":"Current trends in marine algae polysaccharides: The digestive tract, microbial catabolism, and prebiotic potential","volume":"151","author":"Zheng","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.jep.2012.05.046","article-title":"Towards a better understanding of medicinal uses of the brown seaweed Sargassum in traditional Chinese medicine: A phytochemical and pharmacological review","volume":"142","author":"Liu","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10626","DOI":"10.1021\/acs.jafc.0c02555","article-title":"Sargassum fusiforme fucoidan alleviates high-fat diet-induced obesity and insulin resistance associated with the improvement of hepatic oxidative stress and gut microbiota profile","volume":"68","author":"Zhang","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","first-page":"58","article-title":"Komponen bioactive dan aktivitas antioksidan ekstrak kasar Sargassum plagyophyllum","volume":"20","author":"Edison","year":"2020","journal-title":"J. Pengolah. Has. Perikan. Indones."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.1007\/s10811-021-02491-3","article-title":"Potential health benefits of fucoidan from the brown seaweeds Sargassum plagiophyllum and Sargassum polycystum","volume":"33","author":"Saeton","year":"2021","journal-title":"J. Appl. Phycol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.procbio.2012.12.014","article-title":"Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity","volume":"48","author":"Suresh","year":"2013","journal-title":"Process Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kalasariya, H.S., Yadav, V.K., Yadav, K.K., Tirth, V., Algahtani, A., Islam, S., Gupta, N., and Jeon, B.-H. (2021). Seaweed-based molecules and their potential biological activities: An eco-sustainable cosmetics. Molecules, 26.","DOI":"10.3390\/molecules26175313"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kumar, Y., Tarafdar, A., and Badgujar, P.C. (2021). Seaweed as a source of natural antioxidants: Therapeutic activity and food applications. Hindawi, 5753391.","DOI":"10.1155\/2021\/5753391"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.4314\/tjpr.v20i11.16","article-title":"Effects of Sargassum plagiophyllum extract pretreatment on tissue histology of constipated mice","volume":"20","author":"Sengkhim","year":"2021","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.5897\/AJB2007.000-2438","article-title":"Antioxidant activity of extract from a brown alga, Sargassum boveanum","volume":"6","author":"Zahra","year":"2007","journal-title":"Afr. J. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"110715","DOI":"10.1016\/j.nut.2019.110715","article-title":"Amelioration of gut dysbiosis and gastrointestinal motility by konjac oligo-glucomannan on loperamide-induced constipation in mice","volume":"73","author":"Hayeeawaema","year":"2020","journal-title":"Nutrition"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"108821","DOI":"10.1016\/j.biopha.2019.108821","article-title":"Prebiotic oligosaccharides from dragon fruits alter gut motility in mice","volume":"114","author":"Khuituan","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jcf.2004.05.026","article-title":"Transepithelial electrical measurements with the Ussing chamber","volume":"3","author":"Li","year":"2004","journal-title":"J. Cyst. Fibros"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"G1151","DOI":"10.1152\/ajpgi.90649.2008","article-title":"A guide to Ussing chamber studies of mouse intestine","volume":"296","author":"Clarke","year":"2009","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"603","DOI":"10.17576\/jsm-2020-4903-15","article-title":"Effects of plant oligosaccharides derived from dragon fruit on gut microbiota in proximal and distal colon of mice","volume":"49","author":"Peerakietkhajorn","year":"2020","journal-title":"Sains Malays."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1128\/aem.63.4.1268-1273.1997","article-title":"Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCR","volume":"63","author":"Kaufmann","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1128\/AEM.70.1.167-173.2004","article-title":"Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria","volume":"70","author":"Matsuki","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/j.1472-765X.2006.01893.x","article-title":"Comparison of agar plate and real-time PCR on enumeration of Lactobacillus, Clostridium perfringens and total anaerobic bacteria in dog faeces","volume":"42","author":"Fu","year":"2006","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1128\/AEM.68.2.673-690.2002","article-title":"Culture-independent analysis of gut bacteria: The pig gastrointestinal tract microbiota revisited","volume":"68","author":"Leser","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1128\/AEM.66.5.2263-2266.2000","article-title":"Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization","volume":"66","author":"Sghir","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1016\/j.biopha.2017.11.119","article-title":"Enteromorpha and polysaccharides from enteromorpha ameliorate loperamide-induced constipation in mice","volume":"96","author":"Ren","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cherry, P., Yadav, S., Strain, C.R., Allsopp, P.J., Mcsorley, E.M., Ross, R.P., and Stanton, C. (2019). Prebiotics from seaweeds: An ocean of opportunity?. Mar. Drugs, 17.","DOI":"10.3390\/md17060327"},{"key":"ref_48","unstructured":"Gibson, G.R. (2013). Role of the GI tract microbiota in health and disease. Probiotics, Prebiotics and the Gut Microbiota, International Life Science Institute Europe."},{"key":"ref_49","first-page":"979","article-title":"Bifidobacteria and butyrate-producing colon bacteria: Importance and strategies for their stimulation in the human gut","volume":"7","author":"Selak","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1111\/nmo.12425","article-title":"The Short chain fatty acids, butyrate and propionate, have differential effects on the motility of the guinea pig colon","volume":"26","author":"Hurst","year":"2014","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S1095-6433(00)00194-X","article-title":"Decreased colonic mucus in rats with loperamide-induced constipation","volume":"126","author":"Shimotoyodome","year":"2000","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1136\/gut.46.2.218","article-title":"Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon","volume":"46","author":"Barcelo","year":"2000","journal-title":"Gut"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Liu, J., Kandasamy, S., Zhang, J., Kirby, C.W., Karakach, T., Hafting, J., Critchley, A.T., Evans, F., and Prithiviraj, B. (2015). Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites. BMC Complement. Altern. Med., 15.","DOI":"10.1186\/s12906-015-0802-5"},{"key":"ref_54","first-page":"121","article-title":"In vivo study on laxative effect of Prunus amygdalus oil","volume":"8","author":"Jabeen","year":"2019","journal-title":"Int. J. Med. Res. Health Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"549","DOI":"10.3389\/fphys.2016.00549","article-title":"Role of quercetin in modulating chloride transport in the intestine","volume":"7","author":"Yu","year":"2016","journal-title":"Front. Physiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5273","DOI":"10.1113\/jphysiol.2013.261909","article-title":"The secret life of CFTR as a calcium-activated chloride channel","volume":"591","author":"Billet","year":"2013","journal-title":"J. Physiol."},{"key":"ref_57","first-page":"2233","article-title":"Pharmacognostical and phytochemical analysis of Sargassum cinereum (Turner) C. Agardh","volume":"7","author":"Ranjani","year":"2018","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"39310","DOI":"10.1074\/jbc.M406787200","article-title":"Cellular mechanism for potentiation of Ca2+-mediated Cl\u2212 secretion by the flavonoid baicalein in intestinal epithelia","volume":"279","author":"Yue","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3727","DOI":"10.18632\/oncotarget.23197","article-title":"Molecular mechanisms of calcium signaling in the modulation of small intestinal ion transports and bicarbonate secretion","volume":"9","author":"Yang","year":"2018","journal-title":"Oncotarget"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1152\/ajprenal.00631.2014","article-title":"Calmodulin and CaMKII modulate ENaC activity by regulating the association of MARCKS and the cytoskeleton with the apical membrane","volume":"309","author":"Alli","year":"2015","journal-title":"Am. J. Physiol.-Ren. Physiol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/3\/496\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:06:28Z","timestamp":1760133988000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/3\/496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,24]]},"references-count":60,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["nu14030496"],"URL":"https:\/\/doi.org\/10.3390\/nu14030496","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,24]]}}}