{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T22:24:18Z","timestamp":1789597458699,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009553","name":"Czech Health Research Council","doi-asserted-by":"publisher","award":["NU21-09-00362"],"award-info":[{"award-number":["NU21-09-00362"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PROGRES","award":["Q36"],"award-info":[{"award-number":["Q36"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Vegetarian (VG) and vegan (VN) diets in childhood are of growing interest due to their perceived health and environmental benefits. Concerns remain due to the possible disruption of healthy growth and development of children because of the scarcity of evidence-based studies. Among the nutrients of special concern is vitamin B12. Therefore, the Czech Vegan Children Study (CAROTS) decided to examine the relationship between B12 metabolism parameters and B12 intake through diet and supplementation. We analyzed laboratory parameters within n = 79 VG, n = 69 VN, and n = 52 omnivores (OM) children (0\u201318 years old). There were no significant differences in levels of holotranscobalamin (aB12), folate, homocysteine (hcys), or mean corpuscular volume. However, there was a significant difference in levels of cyanocobalamin (B12) (p = 0.018), even though we identified only n = 1 VG and n = 2 VN children as B12 deficient. On the other hand, we identified n = 35 VG, n = 28 VN, and n = 9 OM children with vitamin B12 hypervitaminosis (p = 0.004). This finding was related to a high prevalence of over-supplementation in the group (mean dose for VG 178.19 \u00b1 238.5 \u00b5g per day; VN 278.35 \u00b1 394.63 \u00b5g per day). Additionally, we found a significant (p &lt; 0.05) difference between B12, aB12, and hcys levels of supplemented vs. non-supplemented VG\/VN children. This can show that the intake of vitamin B12 via diet in the VG group might not be sufficient. Secondly, we analyzed a relation between supplement use in pregnancy and breastfeeding and its impact on vitamin B12 levels of children aged 0\u20133 years. Out of n = 46 mothers, only n = 3 (e.g., 6.5%) were not supplemented at all. We have not identified any clinical manifestation of B12 deficiency and only n = 1 child with low serum cobalamin, a child who did not receive vitamin B12 supplementation and whose mother took only low doses of vitamin B12 (25\/\u00b5g\/day).To conclude, we did not observe any life-threatening or severe consequences of laboratory-stated vitamin B12 deficiency; thus, our group was well supplemented. On the other hand, we have identified many subjects with vitamin B12 hypervitaminosis of unknown impact on their health. Further research and new guidelines for B12 supplementation among VG and VN children are needed.<\/jats:p>","DOI":"10.3390\/nu14030535","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T04:49:51Z","timestamp":1643258991000},"page":"535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Cross-Sectional Study of the Prevalence of Cobalamin Deficiency and Vitamin B12 Supplementation Habits among Vegetarian and Vegan Children in the Czech Republic"],"prefix":"10.3390","volume":"14","author":[{"given":"Martin","family":"Sv\u011btni\u010dka","sequence":"first","affiliation":[{"name":"Centre for Research on Diabetes Metabolism, Nutrition of Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Internal Medicine, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Pediatrics, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6655-7683","authenticated-orcid":false,"given":"Anat","family":"Sigal","sequence":"additional","affiliation":[{"name":"Centre for Research on Diabetes Metabolism, Nutrition of Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Pediatrics, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli\u0161ka","family":"Selinger","sequence":"additional","affiliation":[{"name":"Centre for Research on Diabetes Metabolism, Nutrition of Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Internal Medicine, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"},{"name":"Centre for Public Health Promotion, The National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3253-0853","authenticated-orcid":false,"given":"Marina","family":"Henikov\u00e1","sequence":"additional","affiliation":[{"name":"Centre for Research on Diabetes Metabolism, Nutrition of Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Internal Medicine, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"El-Lababidi","sequence":"additional","affiliation":[{"name":"Centre for Research on Diabetes Metabolism, Nutrition of Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Pediatrics, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7995-5947","authenticated-orcid":false,"given":"Jan","family":"Gojda","sequence":"additional","affiliation":[{"name":"Centre for Research on Diabetes Metabolism, Nutrition of Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic"},{"name":"Department of Internal Medicine, Third Faculty of Medicine, University Hospital Kr\u00e1lovsk\u00e9 Vinohrady, Charles University, 10000 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jpeds.2017.04.053","article-title":"Caring for Infants and Children Following Alternative Dietary Patterns","volume":"187","author":"Ferrara","year":"2017","journal-title":"J. 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