{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:13:48Z","timestamp":1781756028006,"version":"3.54.5"},"reference-count":87,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:00:00Z","timestamp":1644278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Ferrous sulphate (FS) is widely used as an iron supplement to treat iron deficiency (ID), but is known to induce inflammation causing gastric side-effects resulting in poor adherence to supplement regimens. Curcumin, a potent antioxidant, has been reported to suppress inflammation via down regulation of NF-\u03baB. The aim of the present double blind, placebo-controlled randomised trial was to assess whether co-administration of FS with a formulated, bioavailable form of curcumin (HydroCurc\u2122) could reduce systemic inflammation and\/or gastrointestinal side-effects. This study recruited 155 healthy participants (79 males; 26.42 years \u00b1 0.55 and 76 females; 25.82 years \u00b1 0.54), randomly allocated to one of five different treatment groups: iron and curcumin placebo (FS0_Plac), low dose (18 mg) iron and curcumin placebo (FS18_Plac), low dose iron and curcumin (FS18_Curc), high dose (65 mg) iron and curcumin placebo (FS65_Plac), and high dose iron and curcumin (FS65_Curc). Completed questionnaires and blood samples were collected from all participants at baseline (day 1), mid-point (day 21), and at end-point (day 42). Results showed a significant reduction in IL-6 in the FS65_Curc group (0.06 pg\/mL \u00b1 0.02, p = 0.0073) between the mid-point and end-point. There was also a significant reduction in mean plasma TNF levels in the FS65_Curc (0.65 pg\/mL \u00b1 0.17, p = 0.0018), FS65_Plac (0.39 pg\/mL \u00b1 0.15, p = 0.0363), and FS18_Curc (0.35 pg\/mL \u00b1 0.13, p = 0.0288) groups from mid-point to end-point. A significant increase was observed in mean plasma TBARS levels (0.10 \u00b5M \u00b1 0.04, p = 0.0283) in the F18_Plac group from baseline to end-point. There was a significant association with darker stools between FS0_Plac vs. FS65_Plac (p = 0.002, Fisher\u2019s exact test) suggesting that high iron dose in the absence of curcumin leads to darker stools. A reduction in inflammation-related markers in response to co-administering supplemental iron alongside formulated curcumin suggests a reduction in systemic inflammation. This supplementation approach may therefore be a more cost effective and convenient alternative to current oral iron-related treatments, with further research to be conducted.<\/jats:p>","DOI":"10.3390\/nu14030712","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T23:42:20Z","timestamp":1644363740000},"page":"712","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Co-Administration of Iron and Bioavailable Curcumin Reduces Levels of Systemic Markers of Inflammation and Oxidative Stress in a Placebo-Controlled Randomised Study"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9754-0737","authenticated-orcid":false,"given":"Helena","family":"Tiekou Lorinczova","sequence":"first","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6878-9314","authenticated-orcid":false,"given":"Gulshanara","family":"Begum","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Temouri","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Renshaw","sequence":"additional","affiliation":[{"name":"Centre for Sport, Exercise and Life Sciences, Institute for Health and Wellbeing, Coventry University, Priory Street, Coventry CV1 5FB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Gulrez","family":"Zariwala","sequence":"additional","affiliation":[{"name":"Centre for Nutraceuticals, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/S1357-2725(01)00063-2","article-title":"Chemistry and biology of eukaryotic iron metabolism","volume":"33","author":"Aisen","year":"2001","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0098-2997(00)00006-6","article-title":"The roles of iron in health and disease","volume":"22","author":"Lieu","year":"2001","journal-title":"Mol. Asp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/S0140-6736(15)60865-0","article-title":"Iron Deficiency Anaemia","volume":"387","author":"Lopez","year":"2016","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/S0140-6736(17)32366-8","article-title":"Global, regional, and national comparative risk assessment of 84 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990-2016: A systematic analysis for the Global Burden of Disease Study 2016","volume":"390","author":"Gakidou","year":"2017","journal-title":"Lancet"},{"key":"ref_5","unstructured":"World Health Organization (2017). Nutritional Anaemias: Tools for Effective Prevention and Control, WHO."},{"key":"ref_6","first-page":"CD009747","article-title":"Daily iron supplementation for improving iron status and health among menstruating women (Protocol)","volume":"4","author":"Pasricha","year":"2012","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1136\/gut.2003.035758","article-title":"Iron, anaemia, and inflammatory bowel diseases","volume":"53","author":"Gasche","year":"2004","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1002\/ibd.20285","article-title":"Guidelines on the diagnosis and management of iron deficiency and anemia in inflammatory bowel diseases","volume":"13","author":"Gasche","year":"2007","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"676S","DOI":"10.1093\/jn\/131.2.676S","article-title":"Iron deficiency and reduced work capacity: A critical review of the research to determine a causal relationship","volume":"131","author":"Haas","year":"2001","journal-title":"J. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1093\/ajcn\/75.4.734","article-title":"Marginal iron deficiency without anemia impairs aerobic adaptation among previously untrained women","volume":"75","author":"Brownlie","year":"2002","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_11","first-page":"2087","article-title":"Iron deficiency and cognitive functions","volume":"10","year":"2014","journal-title":"Neuropsychiatr. Dis. Treat."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1023\/A:1008978114650","article-title":"Iron deficiency, general health and fatigue: Results from the australian longitudinal study on women\u2019s health","volume":"9","author":"Patterson","year":"2000","journal-title":"Qual. Life Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.ijcard.2014.03.169","article-title":"Iron deficiency and health-related quality of life in chronic heart failure: Results from a multicenter European study","volume":"174","author":"Enjuanes","year":"2014","journal-title":"Int. J. Cardiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1185\/03007995.2012.761599","article-title":"Tolerability of different oral iron supplements: A systematic review","volume":"29","author":"Palacios","year":"2013","journal-title":"Curr. Med. Res. Opin."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Tolkien, Z., Stecher, L., Mander, A.P., Pereira, D.I.A., and Powell, J.J. (2015). Ferrous sulfate supplementation causes significant gastrointestinal side-effects in adults: A systematic review and meta-analysis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0117383"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1093\/jn\/131.10.3146","article-title":"Iron supplementation increases disease activity and vitamin e ameliorates the effect in rats with dextran sulfate sodium-induced colitis","volume":"132","author":"Carrier","year":"2002","journal-title":"J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/ajcn\/69.2.250","article-title":"Oral ferrous sulfate supplements increase the free radical\u2013generating capacity of feces from healthy volunteers","volume":"69","author":"Lund","year":"1999","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.3945\/jn.109.111104","article-title":"Antioxidant-rich oral supplements attenuate the effects of oral iron on in situ oxidation susceptibility of human feces","volume":"140","author":"Orozco","year":"2010","journal-title":"J. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"271","DOI":"10.3945\/jn.111.148643","article-title":"Iron depletion and repletion with ferrous sulfate or electrolytic iron modifies the composition and metabolic activity of the gut microbiota in rats","volume":"142","author":"Dostal","year":"2012","journal-title":"J. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kortman, G.A.M., Boleij, A., Swinkels, D.W., and Tjalsma, H. (2012). Iron availability increases the pathogenic potential of salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0029968"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1136\/gut.2010.216929","article-title":"Depletion of luminal iron alters the gut microbiota and prevents Crohn\u2019s disease-like ileitis","volume":"60","author":"Werner","year":"2011","journal-title":"Gut"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.3945\/ajcn.110.004564","article-title":"The effects of iron fortification on the gut microbiota in African children: A randomized controlled trial in C\u00f4te d\u2019Ivoire","volume":"92","author":"Zimmermann","year":"2010","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Asperti, M., Gryzik, M., Brilli, E., Castagna, A., Corbella, M., Gottardo, R., Girelli, D., Tarantino, G., Arosio, P., and Poli, M. (2018). Sucrosomial\u00ae iron supplementation in mice: Effects on blood parameters, hepcidin, and inflammation. Nutrients, 10.","DOI":"10.3390\/nu10101349"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.2147\/DDDT.S81863","article-title":"Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats","volume":"9","author":"Toblli","year":"2015","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s10534-010-9335-z","article-title":"Lactoferrin efficacy versus ferrous sulfate in curing iron deficiency and iron deficiency anemia in pregnant women","volume":"23","author":"Paesano","year":"2010","journal-title":"BioMetals"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"386","DOI":"10.2215\/CJN.02840608","article-title":"Ferumoxytol as an intravenous iron replacement therapy in hemodialysis patients","volume":"4","author":"Provenzano","year":"2009","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1172\/JCI118205","article-title":"Iron supplementation generates hydroxyl radical in vivo. An ESR spin-trapping investigation","volume":"96","author":"Kadiiska","year":"1995","journal-title":"J. Clin. Investig."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1046\/j.1365-2362.2000.00650.x","article-title":"Iron supplementation affects the production of pro-inflammatory cytokines in IL-10 deficient mice","volume":"30","author":"Oldenburg","year":"2000","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_29","first-page":"394","article-title":"Iron supplementation may aggravate inflammatory status of colitis in a rat model","volume":"45","author":"Reifen","year":"2000","journal-title":"Am. J. Dig. Dis."},{"key":"ref_30","first-page":"606","article-title":"Tolerability of iron: A comparison of bis-glycino iron II and ferrous sulfate","volume":"13","author":"Coplin","year":"1991","journal-title":"Clin. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.freeradbiomed.2010.01.003","article-title":"Green tea averts age-dependent decline of hippocampal signaling systems related to antioxidant defenses and survival","volume":"48","author":"Carvalho","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/978-3-540-88201-5_31","article-title":"Structural and Spectral Properties of Curcumin and Metal-Curcumin Complex Derived from Turmeric (Curcuma Longa)","volume":"Volume 127","author":"Bich","year":"2009","journal-title":"Physics and Engineering of New Materials"},{"key":"ref_33","first-page":"117","article-title":"Studies on spice principles as antioxidants in the inhibition of lipid peroxidation of rat liver microsomes","volume":"111","author":"Reddy","year":"1992","journal-title":"Mol. Cell. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/0378-5173(93)90194-K","article-title":"Studies on curcumin and curcuminoids. XXIII: Effects of curcumin on liposomal lipid peroxidation","volume":"90","author":"Smistad","year":"1993","journal-title":"Int. J. Pharm."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0020-1693(01)00687-9","article-title":"Curcuminoids as potential new iron-chelating agents: Spectroscopic, polarographic and potentiometric study on their Fe(III) complexing ability","volume":"328","author":"Borsari","year":"2002","journal-title":"Inorg. Chim. Acta"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/S1386-1425(03)00344-5","article-title":"Spectrophotometric and Electrochemical Determination of the Formation Constants of the Complexes Curcumin-Fe(III)-Water and Curcumin-Fe(II)-Water","volume":"60","year":"2004","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jinorgbio.2014.06.002","article-title":"Curcumin derivatives as metal-chelating agents with potential multifunctional activity for pharmaceutical applications","volume":"139","author":"Ferrari","year":"2014","journal-title":"J. Inorg. Biochem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"469","DOI":"10.2174\/157340607781745447","article-title":"Curcumin contributes to in vitro removal of non-transferrin bound iron by deferiprone and desferrioxamine in thalassemic plasma","volume":"3","author":"Srichairatanakool","year":"2007","journal-title":"Med. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.redox.2014.01.018","article-title":"Curcumin may impair iron status when fed to mice for six months","volume":"2","author":"Chin","year":"2014","journal-title":"Redox Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3389\/fnut.2018.00087","article-title":"The role of polyphenols in human health and food systems: A mini-review","volume":"5","author":"Cory","year":"2018","journal-title":"Front. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Samba-Mondonga, M., Constante, M., Fragoso, G., Calv\u00e9, A., and Santos, M.M. (2019). Curcumin induces mild anemia in a DSS-induced colitis mouse model maintained on an iron-sufficient diet. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0208677"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/09637480802084844","article-title":"Inhibitory effects of spices and herbs on iron availability","volume":"60","author":"Tuntipopipat","year":"2009","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Lorinczova, H., Begum, G., Renshaw, D., and Zariwala, M. (2021). Acute Administration of Bioavailable Curcumin Alongside Ferrous Sulphate Supplements Does Not Impair Iron Absorption in Healthy Adults in a Randomised Trial. Nutrients, 13.","DOI":"10.3390\/nu13072300"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1007\/s00394-018-1766-2","article-title":"Increased bioavailability of curcumin using a novel dispersion technology system (LipiSperse\u00ae)","volume":"58","author":"Briskey","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"28556","DOI":"10.1074\/jbc.M111.256180","article-title":"Curcumin Modulates Nuclear Factor KB (Nf-KB)-Mediated Inflammation in Human Tenocytes in Vitro: Role of the Phosphatidylinositol 3-Kinase\/Akt Pathway","volume":"286","author":"Buhrmann","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_46","first-page":"4880","article-title":"Anacardic Acid (6-Nonadecyl Salicylic Acid), an Inhibitor of Histone Acetyltransferase, Suppresses Expression of Nuclear Factor-\u03baB-Regulated Gene Products Involved in Cell Survival, Proliferation, Invasion, and Inflammation through Inhibition of the Inhibitory Subunit of Nuclear Factor-\u03baB\u03b1 Kinase, Leading to Potentiation of Apoptosis","volume":"111","author":"Sung","year":"2008","journal-title":"Blood J. Am. Soc. Hematol."},{"key":"ref_47","first-page":"1894","article-title":"Pharmacodynamic and pharmacokinetic study of oral Curcuma extract in patients with colorectal cancer","volume":"7","author":"Sharma","year":"2001","journal-title":"Clin. Cancer Res."},{"key":"ref_48","unstructured":"(2021, May 12). Study Randomizer App. Studyrandomizer.Com. Available online: https:\/\/app.studyrandomizer.com."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3758\/BF03193146","article-title":"G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences","volume":"39","author":"Faul","year":"2007","journal-title":"Behav. Res. Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.3758\/BRM.41.4.1149","article-title":"Statistical power analyses using G*Power 3.1: Tests for correlation and regression analyses","volume":"41","author":"Faul","year":"2009","journal-title":"Behav. Res. Methods"},{"key":"ref_51","unstructured":"Dooley, J., and Worwood, M. (2000). Guidelines on Diagnosis and Therapy: Genetic Haemochromatosis, British Committee for Standards in Haematology."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/bjh.15164","article-title":"Diagnosis and therapy of genetic haemochromatosis (review and 2017 update)","volume":"181","author":"Fitzsimons","year":"2018","journal-title":"Br. J. Haematol."},{"key":"ref_53","unstructured":"(2021, May 12). Blood Pressure UK. Available online: http:\/\/www.bloodpressureuk.org\/your-blood-pressure\/understanding-your-bloodpressure\/what-do-the-numbers-mean\/."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1093\/eurheartj\/ehy339","article-title":"2018 ESC\/ESH Guidelines for the management of arterial hypertension. The Task Force for the management of arterial hypertension of the European Society of Cardiology (ESC) and the European Society of Hypertension (ESH)","volume":"39","author":"Williams","year":"2018","journal-title":"Eur. Heart J."},{"key":"ref_55","first-page":"129","article-title":"Symptom frequency and development of a generic functional disorder symptom scale suitable for use in studies of patients with irritable bowel syndrome, fibromyalgia syndrome or chronic fatigue syndrome","volume":"5","author":"Hyland","year":"2019","journal-title":"Chronic Dis. Transl. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Lorinczova, H.T., Fitzsimons, O., Mursaleen, L., Renshaw, D., Begum, G., and Zariwala, M.G. (2020). Co-Administration of Iron and a Bioavailable Curcumin Supplement Increases Serum BDNF Levels in Healthy Adults. Antioxidants, 9.","DOI":"10.3390\/antiox9080645"},{"key":"ref_57","first-page":"782","article-title":"Evaluation of the ABX Pentra 400: A newly available clinical chemistry analyser","volume":"43","author":"Marson","year":"2005","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/clinchem\/40.4.625","article-title":"Automated Latex Agglutination Immunoassay of Serum Ferritin with a Centrifugal Analyzer","volume":"40","author":"Joven","year":"1994","journal-title":"Clin. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/bs.acc.2016.03.002","article-title":"Transferrin Saturation: A Body Iron Biomarker","volume":"Volume 75","author":"Elsayed","year":"2016","journal-title":"Advances in Clinical Chemistry"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/ijlh.12522","article-title":"Performance evaluation of the Sysmex\u00aeXP-300 in an oncology setting: Evaluation and comparison of hematological parameters with the Sysmex\u00aeXN-3000","volume":"38","author":"Louagie","year":"2016","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1001\/archneur.1989.00520460115022","article-title":"The Fatigue Severity Scale: Application to Patients With Multiple Sclerosis and Systemic Lupus Erythematosus","volume":"46","author":"Krupp","year":"1989","journal-title":"Arch. Neurol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1080\/14034940410028406","article-title":"Fatigue in the general population: A translation and test of the psychometric properties of the Norwegian version of the fatigue severity scale","volume":"33","author":"Lerdal","year":"2005","journal-title":"Scand. J. Public Health"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1093\/sleep\/31.11.1601","article-title":"Validation of the Fatigue Severity Scale in a Swiss Cohort","volume":"31","author":"Valko","year":"2008","journal-title":"Sleep"},{"key":"ref_64","first-page":"412964","article-title":"Reliability, Responsiveness, and Validity of the Visual Analog Fatigue Scale to Measure Exertion Fatigue in People with Chronic Stroke: A Preliminary Study","volume":"2010","author":"Tseng","year":"2010","journal-title":"Stroke Res. Treat."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"320","DOI":"10.4082\/kjfm.2012.33.5.320","article-title":"Improved Chronic Fatigue Symptoms after Removal of Mercury in Patient with Increased Mercury Concentration in Hair Toxic Mineral Assay: A Case","volume":"33","author":"Shin","year":"2012","journal-title":"Korean J. Fam. Med."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Pereira, D.I., Irving, S.S.C., Lomer, M.C., and Powell, J.J. (2014). A rapid, simple questionnaire to assess gastrointestinal symptoms after oral ferrous sulphate supplementation. BMC Gastroenterol., 14.","DOI":"10.1186\/1471-230X-14-103"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3102\/00346543042003237","article-title":"Consequences of failure to meet assumptions underlying the fixed effects analyses of variance and covariance","volume":"42","author":"Glass","year":"1972","journal-title":"Rev. Educ. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"315","DOI":"10.3102\/10769986017004315","article-title":"Summarizing Monte Carlo results in methodological research: The one-and two-factor fixed effects ANOVA cases","volume":"17","author":"Harwell","year":"1992","journal-title":"J. Educ. Stat."},{"key":"ref_69","first-page":"579","article-title":"Consequences of assumption violations revisited: A quantitative review of alternatives to the one-way analysis of variance F test","volume":"66","author":"Lix","year":"1996","journal-title":"Rev. Educ. Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/clinchem\/44.1.45","article-title":"Clinical utility of the soluble transferrin receptor and comparison with serum ferritin in several populations","volume":"44","author":"Mast","year":"1998","journal-title":"Clin. Chem."},{"key":"ref_71","first-page":"w14434","article-title":"The treatment of iron deficiency without anaemia (in otherwise healthy persons)","volume":"147","year":"2017","journal-title":"Swiss Med. Wkly."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1000456","DOI":"10.15761\/CCRR.1000456","article-title":"Iron Deficiency Without Anemia\u2014Common, Important, Neglected","volume":"5","author":"Soppi","year":"2019","journal-title":"Clin. Case Rep. Rev."},{"key":"ref_73","unstructured":"World Health Organisation (WHO) (2020, July 09). Serum Ferritin Concentrations for the Assessment of Iron Status and Iron Deficiency in Populations. Vitamin and Mineral Nutrition Information System (WHO\/NMH\/NHD\/MNM\/11.2). Available online: https:\/\/www.who.int\/vmnis\/indicators\/serum_ferritin.pdf."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.ejim.2007.09.024","article-title":"A ferritin level >50 \u03bcg\/L is frequently consistent with iron deficiency","volume":"20","author":"Koulaouzidis","year":"2009","journal-title":"Eur. J. Intern. Med."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1016\/j.ijnurstu.2011.02.019","article-title":"Psychometric properties of the Fatigue Severity Scale\u2014Rasch analyses of individual responses in a Norwegian stroke cohort","volume":"48","author":"Lerdal","year":"2011","journal-title":"Int. J. Nurs. Stud."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"256879","DOI":"10.1155\/2014\/256879","article-title":"Association between Oxidative Stress and Outcome in Different Subtypes of Acute Ischemic Stroke","volume":"2014","author":"Tsai","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"5701","DOI":"10.4049\/jimmunol.178.9.5701","article-title":"TNF Trafficking to Human Mast Cell Granules: Mature Chain-Dependent Endocytosis","volume":"178","author":"Olszewski","year":"2007","journal-title":"J. Immunol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1126\/science.7678183","article-title":"Adipose Expression of Tumor Necrosis Factor-\u03b1: Direct Role in Obesity-Linked Insulin Resistance","volume":"259","author":"Hotamisligil","year":"1993","journal-title":"Science"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2147\/tcrm.2007.3.1.99","article-title":"Etanercept in the treatment of rheumatoid arthritis","volume":"3","author":"Haraoui","year":"2007","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"520258","DOI":"10.1155\/2010\/520258","article-title":"The role of exercise-induced myokines in muscle homeostasis and the defense against chronic diseases","volume":"2010","author":"Brandt","year":"2010","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1210\/en.2003-1319","article-title":"Reduced Exercise Endurance in Interleukin-6-Deficient Mice","volume":"145","author":"Wernstedt","year":"2004","journal-title":"Endocrinology"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1038\/s41591-020-1051-9","article-title":"An inflammatory cytokine signature predicts COVID-19 severity and survival","volume":"26","author":"Huang","year":"2020","journal-title":"Nat. Med."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1172\/JCI200318921","article-title":"C-reactive protein: A critical update","volume":"111","author":"Pepys","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1096\/fj.12-213728","article-title":"Attenuation of plasma annexin A1 in human obesity","volume":"27","author":"Kosicka","year":"2013","journal-title":"FASEB J."},{"key":"ref_85","unstructured":"Janeway, C.A., Travers, P., Walport, M., and Shlomchik, M. (2005). Chapter 14: Manipulation of the immune response. Immunobiology: The Immune System in Health & Disease, Garland Science Publishing. [6th ed.]."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1111\/j.1600-0609.1978.tb00374.x","article-title":"Serum Ferritin during Infection. A longitudinal study","volume":"21","author":"Killander","year":"1978","journal-title":"Scand. J. Haematol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1080\/00365520902839667","article-title":"Intravenous iron sucrose is superior to oral iron sulphate for correcting anaemia and restoring iron stores in IBD patients: A randomized, controlled, evaluator-blind, multicentre study","volume":"44","author":"Lindgren","year":"2009","journal-title":"Scand. J. Gastroenterol."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/3\/712\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:16:12Z","timestamp":1760134572000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/14\/3\/712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,8]]},"references-count":87,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["nu14030712"],"URL":"https:\/\/doi.org\/10.3390\/nu14030712","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,8]]}}}